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Team:Groningen/Template/MODULE/Notebook/secretion/week3
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| - | + | Obtaining all the BioBricks needed for the secretion system to destroy <i>P. aeruginosa</i> and <i>S. aureus</i> | |
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| + | <div class="item">Performing three transformation to <i>E. coli</i>, one with promoter P2 (<a href="http://parts.igem.org/Part: BBa_I746104">BBa_I746104</a>), one with <i>lasI</i> (<a href="http://parts.igem.org/Part:BBa_K081009">BBa_K081009</a>), and one with <i>aiiA</i> (<a href="http://parts.igem.org/Part:BBa_C0060">BBa_C0060</a>)</div> | ||
| + | <div class="item">The tranformated <i>E. coli</i> was divided in several concentrations over LB agar plates with Chloramphenicol</div> | ||
| + | <div class="item">Three glycerol stocks of <i>E. coli</i> DH5α containing the RBS (<a href="http://parts.igem.org/Part:BBa_ B0034">BBa_ B0034</a>), the double terminator (<a href="http://parts.igem.org/Part:BBa_ B0015">BBa_ B0015</a>) and the promoter LasR (<a href="http://parts.igem.org/Part:BBa_ R0079">BBa_ R0079</a>), were inoculated in LB medium, and grown overnight</div> | ||
| + | <div class="item">The plasmids were isolated out of the overnight cultures, and checked on gel</div> | ||
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Latest revision as of 22:06, 17 October 2014
August 6 - August 8
Obtaining all the BioBricks needed for the secretion system to destroy P. aeruginosa and S. aureus
Performing three transformation to E. coli, one with promoter P2 (BBa_I746104), one with lasI (BBa_K081009), and one with aiiA (BBa_C0060)
The tranformated E. coli was divided in several concentrations over LB agar plates with Chloramphenicol
Three glycerol stocks of E. coli DH5α containing the RBS (BBa_ B0034), the double terminator (BBa_ B0015) and the promoter LasR (BBa_ R0079), were inoculated in LB medium, and grown overnight
The plasmids were isolated out of the overnight cultures, and checked on gel
