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Team:Groningen/Template/MODULE/Notebook/bandage/week3
From 2014.igem.org
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| - | + | July 21- July 25 | |
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| - | + | Growing a new overnight cultures and preparing the chemicals for the first polyacrylamide gels with <i>L. lactis</i> cells | |
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| - | + | Pouring a polyacrylamide hydrogel with <i>Lactococcus lactis</i> NZ9700 in it | |
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| - | + | Four polyacrylamide gels with different concentration were poured, one of 10 %, 15 %, 20 %, 25 %, and 30 % acrylamide, we decided to continue with 20 % for now | |
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| + | Pouring a polyacrylamide gel containing an overnight culture, this gel is divided in four, two gels were freeze dried, and two more were incubated overnight at 30 °C with fresh M17 medium | ||
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| + | These gels were checked by phase contrast microscopy after incubation overnight, a lot of cells were visible, but we could not distinguish the living of dead bacteria, therefore we decided to continue the experiment with an inducable GFP expressing strain | ||
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Latest revision as of 01:24, 18 October 2014
July 21- July 25
Growing a new overnight cultures and preparing the chemicals for the first polyacrylamide gels with L. lactis cells
Pouring a polyacrylamide hydrogel with Lactococcus lactis NZ9700 in it
Four polyacrylamide gels with different concentration were poured, one of 10 %, 15 %, 20 %, 25 %, and 30 % acrylamide, we decided to continue with 20 % for now
Pouring a polyacrylamide gel containing an overnight culture, this gel is divided in four, two gels were freeze dried, and two more were incubated overnight at 30 °C with fresh M17 medium
These gels were checked by phase contrast microscopy after incubation overnight, a lot of cells were visible, but we could not distinguish the living of dead bacteria, therefore we decided to continue the experiment with an inducable GFP expressing strain
