Team:UC-Santa Cruz-BioE/Team

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<a href=""><img class="hover" src="https://static.igem.org/mediawiki/2014/8/82/UcsantacruzbioEproject.png" alt="Project" title="Project" style="border-width:0;width:418px;height:61px;"><span><img src="https://static.igem.org/mediawiki/2014/8/82/UcsantacruzbioEproject.png" id="TextArt1" alt="Our Project" title="Our Project" style="border-width:0;width:418px;height:61px;"></span></a></div>
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<span style="color:#191970;font-family:Arial;font-size:16px;"><h1>Designing a Microbial Fuel Cell</h1><!..A HEADING!..></span></div>
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<h3>Team Leader: Aaron Maloney</h3>
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    A microbial fuel cell (MFC) uses bacteria to break down organic compounds found in waste water and generate an electric current. This is a sustainable way to generate power from waste material, with the potential of achieving over 50% energy efficiency. Our project will focus on genetically engineering the bacteria Shewanella oneidensis in ways that will make the microbial fuel cell more efficient.
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<p>Aaron is the primary coordinator of this project. Much of the work couldn't have been done without him. He'll be our representative at the Igem Jamboree.</p>
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According to the 2013 International Energy Outlook, energy demands will increase 56% by 2040. This rapidly growing demand for energy has sparked a search for sustainable and renewable energy sources. While many technologies are being developed to address this, some of the most intriguing are bioelectrochemical systems like the microbial fuel cell (MFC). The distinctiveness of bioelectrochemical systems come from their ability to simultaneously take on two ecological vices with: sustainable energy production, and waste-water treatment. The basics of how bacteria in an MFC produce electricity are understood, however we feel more research needs to be done to increase efficiency at the microbial level.
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    The most popular research for microbial fuel cells has been poised towards increasing the power density using more state-of-the-art synthetic materials in the structural design. Our project will focus on another aspect: modifying the microbes which are responsible for generating the electricity. Many of the current limiting factors of MFC performance comes from the bacteria themselves. We plan to address these factors with our two project goals.
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<h3>Team Captain: Sai Edara</h3>
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    The first and most apparent limiting factor in an MFC is the surface area of the anode. The bacteria can only generate electricity when it is in direct contact with, or in close proximity to the surface of the anode. To take full advantage of the limited surface area, it is best for the bacteria to grow in a dense film called a biofilm. This leads to our first goal:
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<li>1. Increase biofilm growth rate of <i>Shewanella oneidensis</i> on the MFC anode.</li>
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<p>We will also focus on the bacteria's ability to transfer electrons from the compounds in the waste water to the anode. There are many pathways which the bacteria can use to facilitate this, however studies have shown that Shewanella does not utilize them in the most efficient way. Much of inefficiency is due to the generation an excretion of Acetate. This is our second goal:
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<p>Sai has been pursuing a Bioengineering degree since 2011. His leadership role stems from the desire to create and to influence the world in a positive direction. He also refused to list any of his major accomplishments to add to this wikipage. He's a humble guy.</p>
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<li> 2.Alter the metabolism used by the bacteria to utilize the energy lost by Acetate generation</li>
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<h3>Our Advisor:Professor Yat Li</h3>
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<h3>References </h3>
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<p>Professor Yat Li is an Associate Professor of Chemistry at UC Santa Cruz. He received his Ph.D at the University of Hong Kong and completed a Postdoctoral Fellowship at Harvard University. He's the recipient of many awards for past accomplishments, and has graciously decided to help guide us in the design of a microbial fuel cell.</p>
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<h3>Team Captain:Marshall Porter</h3>
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<p>Marshall obtained both <i>Shewanella</i> and the supplies and reagents needed to make our MFC. He was responsible for setting up the microscope as well.</p>
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<li>Korneel Rabaey, ed. Bioelectrochemical systems: from extracellular electron transfer to biotechnological application. IWA publishing, 2010.</li>
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<li>Franks, Ashley E., and Kelly P. Nevin. "Microbial fuel cells, a current review." Energies 3.5 (2010): 899-919.</li>
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<li>Brutinel ED, Gralnick JA. Anomalies of the anaerobic tricarboxylic acid cycle in <i>Shewanella oneidensis</i> revealed by Tn-seq. Mol Microbiol. 2012 Oct;86(2):273-83. doi: 10.1111/j.1365-2958.2012.08196.x. Epub 2012 Aug 27. PubMed PMID: 22925268. </li>
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<li>Papagianni M. Recent advances in engineering the central carbon metabolism of industrially important bacteria. Microb Cell Fact. 2012 Apr 30;11:50. doi: 10.1186/1475-2859-11-50. Review. PubMed PMID: 22545791; PubMed Central PMCID: PMC3461431</li>
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<li>Rabaey K, Verstraete W. Microbial fuel cells: novel biotechnology for energy generation. Trends Biotechnol. 2005 Jun;23(6):291-8. Review. PubMed PMID: 15922081. </li>
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<li>Beliaev, Alex S., et al. "Gene and protein expression profiles of <i>Shewanella oneidensis</i> during anaerobic growth with different electron acceptors." Omics: a journal of integrative biology 6.1 (2002): 39-60.</li>
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<li>Thormann, Kai M., et al. "Control of formation and cellular detachment from <i>Shewanella oneidensis</i> MR-1 biofilms by cyclic di-GMP." Journal of Bacteriology 188.7 (2006): 2681-2691.</li>
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<h3 style=" background-color:#FFFFFF;">Additional Resources</h3>
 
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<p style=" background-color:#FFFFFF";>(Referred to in <a href="https://igem.org/Team.cgi?year=2014&team_name=UC-Santa_Cruz-BioE">Abstract</a>)</p>
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<h3>Wiki Designer:Ariah Mackie</h3>
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<p>Ariah is responsible for editing and designing web coverage of the UC-Santa Cruz-BioE project progress. A total novice to html and CSS before this project, she's got her work cut out for her.</p>
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<li>8. Ching, Leang, and Nikhil S. Malvankar. "Engineering Geobacter Sulffureducens to Produce a Highly Cohesive Conductive Matrix with Enhanced Capacity for Current Production." Energy and Environmental Science (2013): 1901-908. Web.</li>
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<h3>Christian Pettet</h3>
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<p>Christian is earning his BS in biomolecular bioengineering. He wrote the protocols, cultured <i>Shewanella</i>, prepared the glycerol stock growth media. He also assisted with the transformation of the bacterium.</p>
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<h3>Arjun Sandhu</h3>
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<p>Arjun is a bioengineering major. He contributed in the design of the protocols, creation of glycerol stocks,culturing the cells, and cell transformation.</p>
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Latest revision as of 01:36, 18 October 2014

Team

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Getting to Know the Group...

Team Leader: Aaron Maloney

Aaron is the primary coordinator of this project. Much of the work couldn't have been done without him. He'll be our representative at the Igem Jamboree.

Team Captain: Sai Edara

Sai has been pursuing a Bioengineering degree since 2011. His leadership role stems from the desire to create and to influence the world in a positive direction. He also refused to list any of his major accomplishments to add to this wikipage. He's a humble guy.

Our Advisor:Professor Yat Li

Professor Yat Li is an Associate Professor of Chemistry at UC Santa Cruz. He received his Ph.D at the University of Hong Kong and completed a Postdoctoral Fellowship at Harvard University. He's the recipient of many awards for past accomplishments, and has graciously decided to help guide us in the design of a microbial fuel cell.

Team Captain:Marshall Porter

Marshall obtained both Shewanella and the supplies and reagents needed to make our MFC. He was responsible for setting up the microscope as well.

Wiki Designer:Ariah Mackie

Ariah is responsible for editing and designing web coverage of the UC-Santa Cruz-BioE project progress. A total novice to html and CSS before this project, she's got her work cut out for her.

Christian Pettet

Christian is earning his BS in biomolecular bioengineering. He wrote the protocols, cultured Shewanella, prepared the glycerol stock growth media. He also assisted with the transformation of the bacterium.

Arjun Sandhu

Arjun is a bioengineering major. He contributed in the design of the protocols, creation of glycerol stocks,culturing the cells, and cell transformation.

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