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| | <head></head> | | <head></head> |
| | <body> | | <body> |
| - | <h2>miRNA</h2> | + | <div style="padding:20px"> |
| - | 6.22<br>
| + | <table width="90%" align="center"> |
| - | Update the parts list on the wiki<br>
| + | |
| - | Create an experiments page on the wiki<br>
| + | <tr><td><h3 align="center" style="font-size:42px; color:teal"><b> NOTEBOOKS</b></h3><br></td></tr> |
| - | Prepare to meet with Jake Beal<br>
| + | <tr><td><p style="font-size:12px" align=center><i>Attributions: James Anderson (Treatment), Gary Burnett (miRNA), <br> Shinjini Saha (Native Receptor), Alex Smith (B-Cell Receptor)</i></p></td></tr> |
| - | Re-transform MAV1212<br>
| + | <tr><td align=center> <img src="https://static.igem.org/mediawiki/2014/7/76/MIT_2014_Notebook_icon.png"> </td></tr> |
| - | Re-transform MAV1212-Hef1a-eBFP2<br>
| + | </table> |
| - | Mini prep MAV1212-Hef1a-eBFP2<br>
| + | <br><br><br> |
| - | 6.23<br>
| + | <center><b> |
| - | Go over workflow of plasmid construction as a group<br>
| + | <h2><a href="https://2014.igem.org/Wiki/2014.igem.org/Team:MIT/native_receptor_notebook" style="color:black">NATIVE RECEPTOR LAB NOTEBOOK</h2></a> |
| - | Finish a unified daily log<br>
| + | <h2><a href="https://2014.igem.org/Wiki/2014.igem.org/Team:MIT/BCR_notebook" style="color:black">B-CELL RECEPTOR LAB NOTEBOOK</h2></a> |
| - | Digest meeting with Jake Beal<br>
| + | < h2><a href="https:// 2014. igem. org/ Wiki/2014. igem. org/Team: MIT/ miRNA_notebook" style="color: black"> miRNA DETECTION LAB NOTEBOOK</ h2></a> |
| - | Go through plates<br>
| + | < h2><a href="https: //2014. igem. org/Wiki/2014. igem. org/ Team: MIT/ Treatment_notebook" style="color: black"> TREATMENT LAB NOTEBOOK</ h2></ a> |
| - | Re plate MAV1212<br>
| + | </b></center> |
| - | Pick colonies for MAV1212<br>
| + | <br><br><br> |
| - | Plan LacZ PCR product<br>
| + | |
| - | Primers ( ? )<br>
| + | |
| - | Figure out what's going on<br>
| + | |
| - | <br>
| + | |
| - | 6.24<br>
| + | |
| - | Present about cell models<br>
| + | |
| - | COMPLETE PARTS LIST ON WIKI<br>
| + | |
| - | Prep experiments<br>
| + | |
| - | Contact Jeremy about multiple miRNA target sites chained together<br>
| + | </div> |
| - | Pick colonies for MAV1212<br>
| + | |
| - | Miniprep MAV1212<br>
| + | |
| - | Keep MAV1212-Hef1a-eBFP2 (for use WITHOUT target sites)<br>
| + | |
| - | Gateway MAV1212-Hef1a-L7ae (for high sensor)<br>
| + | |
| - | Gateway MAV1212_Hef1a-eYFP (for use WITH target sites)<br>
| + | |
| - | Transform Hef1a, MAV1212 ( ? )<br>
| + | |
| - | NOTES:<br>
| + | |
| - | No MAV1212 colonies grew on the plates or in the medium<br>
| + | |
| - | Jeremy gave us more, enough for one more transformation<br>
| + | |
| - | 6.25<br>
| + | |
| - | Create a clear workflow<br>
| + | |
| - | Update "status" of plasmids on parts list<br>
| + | |
| - | How do we string multiple low sensors together?<br>
| + | |
| - | Determine which pairs of target sites we want<br>
| + | |
| - | Find sequences (hopefully under 200 bp)<br>
| + | |
| - | Send ultramer order to Brian<br>
| + | |
| - | Tranform LRs <br>
| + | |
| - | MAV1212-Hef1a-mKate2<br>
| + | |
| - | MAV1212-Hef1a-L7ae<br>
| + | |
| - | Hef1a on Kan<br>
| + | |
| - | Pick colonies from transformations<br>
| + | |
| - | MAV1212-Hef1a-eBFP2<br>
| + | |
| - | Golden Gate<br>
| + | |
| - | Gather miRNA target sites from Jeremy<br>
| + | |
| - | Make all 6 single low sensors<br>
| + | |
| - | NOTES<br>
| + | |
| - | MAV1212 didn't grow (ccdB is over expressed)<br>
| + | |
| - | Hef1a was plated on Amp plates...but it has Kan resistance<br>
| + | |
| - | Verify MAV1212-Hef1a-eBFP2 via sequencing AmpR/eBFP2 region<br>
| + | |
| - | <br>
| + | |
| - | 6.26<br>
| + | |
| - | AD blood cells from PrecisionMed? Talk to Brian?<br>
| + | |
| - | Meet with Kyle about designing siRNA (email sent)<br>
| + | |
| - | Make siRNA sequences in geneious<br>
| + | |
| - | Send order to Brian<br>
| + | |
| - | Picture of how low sensors come together<br>
| + | |
| - | Determine which pairs of target sites we want<br>
| + | |
| - | Find sequences (hopefully under 200 bp)<br>
| + | |
| - | Send ultramer order to Brian<br>
| + | |
| - | Plan new MAV vector<br>
| + | |
| - | Plan high sensor construction (meet with Brian)<br>
| + | |
| - | Golden Gate<br>
| + | |
| - | Gather miRNA target sites from Jeremy<br>
| + | |
| - | Nanodrop for concentrations ( ? )<br>
| + | |
| - | Make all 6 single low sensors (in conjunction with antibody group)<br>
| + | |
| - | Miniprep eBFP2<br>
| + | |
| - | NOTES<br>
| + | |
| - | Both LR's failed (mKate2 and L7ae)<br>
| + | |
| - | Meeting w/ Jeremy tomorrow to talk about new MAV vector and high sensors<br>
| + | |
| - | We need to Gibson in eBFP2 before we can finish the sensors<br>
| + | |
| - | <br>
| + | |
| - | siRNA MEETING WITH KYLE<br>
| + | |
| - | mature (5' phospho) and sense (reverse complement)<br>
| + | |
| - | copy format from 2013<br>
| + | |
| - | send brian both sequences as a duplex (IDT has a duplex option), with all the modification<br>
| + | |
| - | We NEED to Gibson in the eBFP2 control<br>
| + | |
| - | each high sensor on its own transcript (not necessarily own plasmid)<br>
| + | |
| - | QUESTIONS<br>
| + | |
| - | what do the "r" and "m" stand for?<br>
| + | |
| - | -rna and modification<br>
| + | |
| - | where do the overhangs come from? why are they there?<br>
| + | |
| - | -mature one has 2 u's (constant)<br>
| + | |
| - | 6.27<br>
| + | |
| - | Write up low sensor experiment plans<br>
| + | |
| - | Keep updating the parts list<br>
| + | |
| - | Meet with Jeremy<br>
| + | |
| - | Discuss high sensor plans<br>
| + | |
| - | New MAV assembly plan<br>
| + | |
| - | Questions for Brian<br>
| + | |
| - | eBFP2 Gibson for Flow Cytometry ( ? )<br>
| + | |
| - | Send siRNA sequences<br>
| + | |
| - | Miniprep Hef1a<br>
| + | |
| - | Golden Gate all the low sensors<br>
| + | |
| - | NOTES<br>
| + | |
| - | Reasons to not use phages for circuit delivery<br>
| + | |
| - | 6.30<br>
| + | |
| - | Keep updating the parts list<br>
| + | |
| - | Strengthen experiments page<br>
| + | |
| - | Prepare protocol for building new MAV<br>
| + | |
| - | Prepare protocol for building high sensors<br>
| + | |
| - | Transform low sensor Golden Gates<br>
| + | |
| - | Start building new MAV<br>
| + | |
| - | <br>
| + | |
| - | <br>
| + | |
| - | NOTES<br>
| + | |
| - | Meet w/ Brian tomorrow to talk about double input ultramer<br>
| + | |
| - | Co-transfect 2x Kturn & MAV w/ target sites & eBFP2<br>
| + | |
| - | PCR for building new MAV <br>
| + | |
| - | <br>
| + | |
| - | 7.1<br>
| + | |
| - | Keep updating the parts list<br>
| + | |
| - | Meet w/ Brian about double input low sensor<br>
| + | |
| - | Fill out PCR protocol to be ready for tomorrow<br>
| + | |
| - | Create high sensor construction map (unified to look like the other groups)<br>
| + | |
| - | Design primers / find restriction enzymes to sequence low sensors<br>
| + | |
| - | Pick multiple low sensor colonies<br>
| + | |
| - | Re-transform low sensors<br>
| + | |
| - | NOTES<br>
| + | |
| - | Double input low sensor will be a gBlock<br>
| + | |
| - | Lots of blue colonies, not many white colonies (none from GH)<br>
| + | |
| - | <br>
| + | |
| - | 7.2<br>
| + | |
| - | Keep updating the parts tree<br>
| + | |
| - | Propose experiments to test L7ae and Tau14/21<br>
| + | |
| - | Find restriction enzymes to digest original EF<br>
| + | |
| - | Miniprep original EF<br>
| + | |
| - | PCR mRFP out of pL1F_1_S6_S7<br>
| + | |
| - | PCR Purify mRFP<br>
| + | |
| - | Run the gel for mRFP<br>
| + | |
| - | Re Golden Gate EF and GH<br>
| + | |
| - | Digest mRFP cassette<br>
| + | |
| - | Re ligate mRFP cassette<br>
| + | |
| - | Transform mRFP cassette<br>
| + | |
| - | Retransform EF and GH<br>
| + | |
| - | NOTES<br>
| + | |
| - | There were no colonies to pick from EF and GH --> re transform<br>
| + | |
| - | <br>
| + | |
| - | 7.7<br>
| + | |
| - | Keep updating the parts tree (include experiments)<br>
| + | |
| - | Update experiments page<br>
| + | |
| - | Obtain plasmid maps<br>
| + | |
| - | Email Jin about TAL14 and miRNA target sites<br>
| + | |
| - | Talk to Lyla/Alexa about transfecting later this week<br>
| + | |
| - | Look for plasmids<br>
| + | |
| - | pEXPR: UAS-Gal4-TAL14-eYFP<br>
| + | |
| - | pEXPR: Gal4VP16<br>
| + | |
| - | pEXPR: Hef1a-TAL14<br>
| + | |
| - | pEXPR: Hef1a-2x Kturn-eYFP<br>
| + | |
| - | Transformations<br>
| + | |
| - | Low Sensor Golden Gates<br>
| + | |
| - | pEXPR: Hef1a-eBFP2 (186 ng/uL)<br>
| + | |
| - | pEXPR: Hef1a-L7ae (96 ng/uL)<br>
| + | |
| - | Pick red colonies and grow in medium!<br>
| + | |
| - | Find concentrations of eBFP2 and L7ae<br>
| + | |
| - | NOTES<br>
| + | |
| - | Nelson doesn't know where it is, but he needs it as well, so he's on the hunt<br>
| + | |
| - | If we're ready, we can transfect very early next week<br>
| + | |
| - | <br>
| + | |
| - | 7.8<br>
| + | |
| - | Keep updating the parts tree (include experiments)<br>
| + | |
| - | Figure out where the Golden Gates went<br>
| + | |
| - | Create protocol for siRNA transfection<br>
| + | |
| - | Meet with Jin at 3<br>
| + | |
| - | Transformations <br>
| + | |
| - | EF<br>
| + | |
| - | GH<br>
| + | |
| - | Mutant Hef1a-2xKturn-eGFP<br>
| + | |
| - | Wild Type Hef1a-2xKturn-eGFP<br>
| + | |
| - | Pick colonies from L7ae and eBFP2<br>
| + | |
| - | Miniprep MAV1212<br>
| + | |
| - | Digest MAV1212<br>
| + | |
| - | Run gel for MAV1212<br>
| + | |
| - | Image gel for MAV1212<br>
| + | |
| - | NOTES<br>
| + | |
| - | Jin gave us ideas to optimize our high sensors and experiments<br>
| + | |
| - | <br>
| + | |
| - | 7.9<br>
| + | |
| - | Keep updating the parts tree (include experiments)<br>
| + | |
| - | Find new miRNA target site vector. Email:<br>
| + | |
| - | Kyle<br>
| + | |
| - | Lila<br>
| + | |
| - | Samira<br>
| + | |
| - | Obtain plasmid maps<br>
| + | |
| - | Hef1a-2xKturn (mutant and w.t.)<br>
| + | |
| - | GAL4 (from Brian)<br>
| + | |
| - | Pick colonies & Grow in medium<br>
| + | |
| - | EF<br>
| + | |
| - | GH<br>
| + | |
| - | pENTR: L7ae<br>
| + | |
| - | pEXPR: Hef1a-eBFP2<br>
| + | |
| - | Mutant Hef1a-2xKturn-eGFP<br>
| + | |
| - | Wild Type Hef1a-2xKturn-eGFP<br>
| + | |
| - | MAV12112-Hef1a-eBFP2 (control)<br>
| + | |
| - | Create pENTR: TAL14<br>
| + | |
| - | BP Reaction from TAL14<br>
| + | |
| - | Transform TAL14<br>
| + | |
| - | NOTES<br>
| + | |
| - | GH and pENTR: L7ae didn't grow properly (their plates had almost no agar)<br>
| + | |
| - | L7ae was re transformed<br>
| + | |
| - | <br>
| + | |
| - | 7.10<br>
| + | |
| - | Keep updating the parts tree (include experiments)<br>
| + | |
| - | More descriptive names<br>
| + | |
| - | Include other plasmid<br>
| + | |
| - | Alternative backbones? Check iGEM 2013<br>
| + | |
| - | Plan experiment to test Kturn (Just Kturn and L7ae)<br>
| + | |
| - | Re-make double input low sensor as two ultramers (use the Q3 site)<br>
| + | |
| - | Pick colonies & grow in medium (pick 5)<br>
| + | |
| - | Midi prep<br>
| + | |
| - | EF<br>
| + | |
| - | GH<br>
| + | |
| - | pEXPR: Hef1a-eBFP2<br>
| + | |
| - | Mutant Hef1a-2xKturn-eGFP<br>
| + | |
| - | Wild Type Hef1a-2xKturn-eGFP<br>
| + | |
| - | Mini prep<br>
| + | |
| - | pENTR: L7ae<br>
| + | |
| - | GH<br>
| + | |
| - | Mini prep<br>
| + | |
| - | EF<br>
| + | |
| - | pEXPR: Hef1a-eBFP2<br>
| + | |
| - | Mutant Hef1a-2xKturn-eGFP<br>
| + | |
| - | Wild Type Hef1a-2xKturn-eGFP<br>
| + | |
| - | LR<br>
| + | |
| - | MAV1212-Hef1a-L7ae<br>
| + | |
| - | MAV1212-Hef1a-TAL14<br>
| + | |
| - | BP<br>
| + | |
| - | P4-donor-P1R on Brian's bench<br>
| + | |
| - | TAL14-mKate pEST in Brian's fridge<br>
| + | |
| - | Golden Gate<br>
| + | |
| - | GH<br>
| + | |
| - | Transformation<br>
| + | |
| - | TAL14 Promoter (from BP rxn)<br>
| + | |
| - | TAL14-mKate-pest<br>
| + | |
| - | pENTR: L1_TAL14_L2<br>
| + | |
| - | MAV1212-mRFP<br>
| + | |
| - | NOTES<br>
| + | |
| - | We should sequence the low sensors when they're done<br>
| + | |
| - | Golden Gate Calculations<br>
| + | |
| - | <br>
| + | |
| - | 7.11<br>
| + | |
| - | Keep updating the parts tree (include experiments)<br>
| + | |
| - | Alternative backbones? Check iGEM 2013<br>
| + | |
| - | Actual list of experiments<br>
| + | |
| - | TAL14 & GAL4 (to test promoter)<br>
| + | |
| - | MAV1212-hEF1a-eBFP2<br>
| + | |
| - | Make/Find primers<br>
| + | |
| - | To sequence both low sensors<br>
| + | |
| - | Sequence/digest plasmids to verify - may be unnecessary. Emailed Brian.<br>
| + | |
| - | EF<br>
| + | |
| - | GH - don't have this<br>
| + | |
| - | pEXPR: MAV1212-Hef11a-eBFP2<br>
| + | |
| - | Mutant Hef1a-2xKturn-eGFP<br>
| + | |
| - | Wild Type Hef1a-2xKturn-eGFP<br>
| + | |
| - | Pick colonies and grow up<br>
| + | |
| - | MAV1212-mRFP<br>
| + | |
| - | L1_TALER14_L2<br>
| + | |
| - | pTAL14-mKate-pEST<br>
| + | |
| - | MAV1212-Hef1a-eBFP2 for midiprep (pending Brian's approval)<br>
| + | |
| - | EF low sensor for midiprep (pending Brian's approval)<br>
| + | |
| - | Mutant Hef1a-2xKturn-eGFP for midiprep (pending Brian's approval)<br>
| + | |
| - | WT Hef1a-2xKturn-eGFP for midiprep (pending Brian's approval)<br>
| + | |
| - | Miniprep<br>
| + | |
| - | GH - don't have this<br>
| + | |
| - | Transformations<br>
| + | |
| - | GH (Golden Gate)<br>
| + | |
| - | Hef1a-L7ae (LR)<br>
| + | |
| - | Hef1a-TAL14 (LR)<br>
| + | |
| - | BP<br>
| + | |
| - | P4-donor-P1R with TAL14-mKate pEST<br>
| + | |
| - | NOTES<br>
| + | |
| - | BP rxn didn't yield colonies --> re-doing it and letting it grow overnight this time<br>
| + | |
| - | GH plate has no colonies :/<br>
| + | |
| - | <br>
| + | |
| - | 7.14<br> | + | |
| - | Keep updating the parts tree (include experiments)<br>
| + | |
| - | Obtain pEXPR: hEF1a-GAL4VP16<br>
| + | |
| - | Fill out the Genewiz Order Form to sequence Low Sensor miR-144<br>
| + | |
| - | Prepare transfection well plate maps<br>
| + | |
| - | So where are the siRNA though...<br>
| + | |
| - | Why didn't the GH golden gate work on try #3?<br>
| + | |
| - | Change name of high sensors in tree<br>
| + | |
| - | Pick colonies<br>
| + | |
| - | pEXPR: TAL14-mKate<br>
| + | |
| - | Midiprep<br>
| + | |
| - | pEXPR: MAV1212-Hef11a-eBFP2<br>
| + | |
| - | Mutant Hef1a-2xKturn-eGFP<br>
| + | |
| - | Wild Type Hef1a-2xKturn-eGFP<br>
| + | |
| - | Low Sensor miR-144<br>
| + | |
| - | Hef1a-L7ae (LR)<br>
| + | |
| - | Miniprep<br>
| + | |
| - | MAV1212-mRFP<br>
| + | |
| - | L1_TALER14_L2<br>
| + | |
| - | pTAL14-mKate-pEST<br>
| + | |
| - | LR<br>
| + | |
| - | pEXPR: MAV1212-TRE-L7ae<br>
| + | |
| - | NOTES<br>
| + | |
| - | so...the siRNA are nowhere to be found<br>
| + | |
| - | hEF1a-TAL14 (LR) has no colonies<br>
| + | |
| - | TRE is nowhere to be found<br>
| + | |
| - | <br>
| + | |
| - | 7.15<br>
| + | |
| - | Keep updating the parts tree (include experiments)<br>
| + | |
| - | Obtain Plasmids<br>
| + | |
| - | pEXPR: hEF1a-GAL4VP16<br>
| + | |
| - | pENTR: TRE (promoter)<br>
| + | |
| - | pEXPR: TAL14-mKate<br>
| + | |
| - | Troubleshoot<br>
| + | |
| - | So where are the siRNA though... (keep looking around, they were ordered)<br>
| + | |
| - | Why didn't the GH golden gate work on try #3?<br>
| + | |
| - | Retransform products at higher concentration<br>
| + | |
| - | Fill out the Genewiz Order Form to sequence Low Sensor miR-144<br>
| + | |
| - | Design primers to sequence high sensors<br>
| + | |
| - | Ask Jeremy about sequencing primers<br>
| + | |
| - | Look into siRNA transfection<br>
| + | |
| - | TOP PRIORITY: SEQUENCE LOW SENSORS (can't be done till primers arrive)<br>
| + | |
| - | Pick colonies<br>
| + | |
| - | pEXPR: TAL14-mKate<br>
| + | |
| - | LR<br>
| + | |
| - | pEXPR: MAV1212-TRE-L7ae<br>
| + | |
| - | Transfection<br>
| + | |
| - | L7ae experiment<br>
| + | |
| - | Golden Gate<br>
| + | |
| - | High Sensor miR-30d (I13 & J13)<br>
| + | |
| - | High Sensor miR-146a (I5 & J5)<br>
| + | |
| - | High Sensor let-7f (1.13 & 1.14)<br>
| + | |
| - | High Sensor miR-125b (A3 & B3)<br>
| + | |
| - | Combined Low Sensor miR-144-miR-181c<br>
| + | |
| - | Get ultramers from Brian<br>
| + | |
| - | Anneal target sites<br>
| + | |
| - | All high sensors<br>
| + | |
| - | Transformation<br>
| + | |
| - | Re-do Low Sensor miR-181c<br>
| + | |
| - | Midiprep<br>
| + | |
| - | pEXPR: TAL14-mKate<br>
| + | |
| - | NOTES<br>
| + | |
| - | The transfections are a little bit messed up<br>
| + | |
| - | We need to verify the MAV1212-hEF1a-L7ae<br>
| + | |
| - | <br>
| + | |
| - | 7.16<br>
| + | |
| - | How To Please Ron/Brain<br>
| + | |
| - | Keep updating the parts tree (include experiments)<br>
| + | |
| - | miRNA Sensors (Theory)<br>
| + | |
| - | Anaylze Low Sensor miR-144 sequencing results<br>
| + | |
| - | miRNA Sensors (Lab Work)<br>
| + | |
| - | Restriction Digest<br>
| + | |
| - | MAV1212-hEF1a-L7ae / that other shit in the fridge<br>
| + | |
| - | Golden Gate<br>
| + | |
| - | High Sensor miR-30d (I13 & J13)<br>
| + | |
| - | High Sensor miR-146a (I5 & J5)<br>
| + | |
| - | High Sensor let-7f (1.13 & 1.14)<br>
| + | |
| - | High Sensor miR-125b (A3 & B3)<br>
| + | |
| - | Low Sensor miR-144 (E5 & F5)<br>
| + | |
| - | Low Sensor miR-181c (G7 & H7)<br>
| + | |
| - | Low Sensor miR-144-miR-181c<br>
| + | |
| - | Transform<br>
| + | |
| - | pEXPR: MAV1212-TRE-L7ae (LR)<br>
| + | |
| - | pEXPR: MAV1212-hEF1a-GAL4VP16 (LR)<br>
| + | |
| - | pEXPR: MAV1212-TRE-GAL4VP16 (LR)<br>
| + | |
| - | Re-suspend Low Sensor miR-144-181c oligos<br>
| + | |
| - | Anneal Low Sensor miR-44-181c oligos<br>
| + | |
| - | NOTES<br>
| + | |
| - | Low Sensor miR-144 did not pass quality control for sequencing<br>
| + | |
| - | After annealing the double input low sensors, the didn't nanodrop well<br>
| + | |
| - | Golden Gate Calculations<br>
| + | |
| - | Restriction Digest Calculations<br>
| + | |
| - | <br>
| + | |
| - | 7.17<br>
| + | |
| - | �<br>
| + | |
| - | Keep updating the parts tree (include experiments)<br>
| + | |
| - | Troubleshoot<br>
| + | |
| - | Why didn't the oligos nanodrop??<br>
| + | |
| - | miRNA Sensors (Lab Work)<br>
| + | |
| - | Golden Gate<br>
| + | |
| - | Low Sensor miR-144-miR-181c<br>
| + | |
| - | Transform<br>
| + | |
| - | High Sensor miR-30d<br>
| + | |
| - | High Sensor miR-146a<br>
| + | |
| - | High Sensor let-7f<br>
| + | |
| - | High Sensor miR-125b<br>
| + | |
| - | Low Sensor miR-144<br>
| + | |
| - | Low Sensor miR-181c<br>
| + | |
| - | Pick colonies to miniprep and midiprep<br>
| + | |
| - | pEXPR: MAV1212-TRE-L7ae (LR)<br>
| + | |
| - | pEXPR: MAV1212-hEF1a-GAL4VP16 (LR)<br>
| + | |
| - | pEXPR: MAV1212-TRE-GAL4VP16 (LR)<br>
| + | |
| - | Prepare and run a gel (Hyperladder 2 and 4% agaros gel w/ annealed target sites)<br>
| + | |
| - | High Sensor miR-30d TS<br>
| + | |
| - | High Sensor miR-146a TS<br>
| + | |
| - | High Sensor let-7f TS<br>
| + | |
| - | High Sensor miR-125b TS<br>
| + | |
| - | Low Sensor miR-144 TS<br>
| + | |
| - | Low Sensor miR-181c TS<br>
| + | |
| - | FACS cell prep<br>
| + | |
| - | Transfect L7ae Experiment<br>
| + | |
| - | Analyze FACS data<br>
| + | |
| - | NOTES<br>
| + | |
| - | We have a new primer to sequence with (again, from Jeremy)<br>
| + | |
| - | We now have FACS DATA<br>
| + | |
| - | 7.18<br>
| + | |
| - | Keep updating the parts tree (include experiments)<br>
| + | |
| - | Troubleshoot<br>
| + | |
| - | Why didn't the oligos nanodrop??<br>
| + | |
| - | Meet w/ Kyle about FlowJo<br>
| + | |
| - | Look for the siRNA<br>
| + | |
| - | Golden Gate<br>
| + | |
| - | Low Sensor miR-144-miR-181c<br>
| + | |
| - | Pick colonies<br>
| + | |
| - | High Sensor miR-30d<br>
| + | |
| - | High Sensor miR-146a<br>
| + | |
| - | High Sensor let-7f<br>
| + | |
| - | High Sensor miR-125b<br>
| + | |
| - | Low Sensor miR-144<br>
| + | |
| - | Low Sensor miR-181c<br>
| + | |
| - | Miniprep<br>
| + | |
| - | pEXPR: MAV1212-TRE-L7ae (LR)<br>
| + | |
| - | pEXPR: MAV1212-hEF1a-GAL4VP16 (LR)<br>
| + | |
| - | pEXPR: MAV1212-TRE-GAL4VP16 (LR)<br>
| + | |
| - | Restriction Digest & run gel<br>
| + | |
| - | pEXPR: MAV1212-TRE-L7ae (LR)<br>
| + | |
| - | pEXPR: MAV1212-hEF1a-GAL4VP16 (LR)<br>
| + | |
| - | pEXPR: MAV1212-TRE-GAL4VP16 (LR)<br>
| + | |
| - | Midiprep?<br>
| + | |
| - | pEXPR: MAV1212-TRE-L7ae (LR)<br>
| + | |
| - | pEXPR: MAV1212-hEF1a-GAL4VP16 (LR)<br>
| + | |
| - | pEXPR: MAV1212-TRE-GAL4VP16 (LR)<br>
| + | |
| - | Gateway<br>
| + | |
| - | pEXPR: MAV1212-hEF1a-TAL14<br>
| + | |
| - | Transform<br>
| + | |
| - | pENTR: TAL14<br>
| + | |
| - | pENTR: TAL21<br>
| + | |
| - | pENTR: Something for yeast for Brian<br>
| + | |
| - | NOTES<br>
| + | |
| - | The gel didn't work very well, so we're re-doing it tomorrow<br>
| + | |
| - | 7.20<br>
| + | |
| - | Keep updating the parts tree (include experiments)<br>
| + | |
| - | Meet w/ Kyle about FlowJo<br>
| + | |
| - | Troubleshoot<br>
| + | |
| - | What's going on with the double input low sensor<br>
| + | |
| - | Move siRNA into our cryobox<br>
| + | |
| - | Pick colonies<br>
| + | |
| - | High Sensor miR-30d<br>
| + | |
| - | High Sensor miR-146a<br>
| + | |
| - | High Sensor let-7f<br>
| + | |
| - | High Sensor miR-125b<br>
| + | |
| - | Low Sensor miR-144<br>
| + | |
| - | Low Sensor miR-181c<br>
| + | |
| - | pENTR: TAL14<br>
| + | |
| - | pENTR: TAL21<br>
| + | |
| - | pENTR: Something for yeast for Brian<br>
| + | |
| - | Restriction Digest & run gel<br>
| + | |
| - | pEXPR: MAV1212-TRE-L7ae (LR)<br>
| + | |
| - | pEXPR: MAV1212-hEF1a-GAL4VP16 (LR)<br>
| + | |
| - | pEXPR: MAV1212-TRE-GAL4VP16 (LR)<br>
| + | |
| - | Inoculate midiprep culture?<br>
| + | |
| - | If the LR's have the right sequence<br>
| + | |
| - | Transform<br>
| + | |
| - | Re transform MAV1212-hEF1a-TAL14 LR<br>
| + | |
| - | FACS Prep<br>
| + | |
| - | NOTES<br>
| + | |
| - | The digest keeps failing<br>
| + | |
| - | <br>
| + | |
| - | 7.21<br>
| + | |
| - | Keep updating the parts tree (include experiments)<br>
| + | |
| - | Meet w/ Kyle<br>
| + | |
| - | FlowJo<br>
| + | |
| - | Bright Field Images<br>
| + | |
| - | miRNA Sensors (Theory)<br>
| + | |
| - | Troubleshoot<br>
| + | |
| - | What's going on with the double input low sensor<br>
| + | |
| - | Is there a different GG thermal cycler program for Bbs1?<br>
| + | |
| - | Run new transfection plate/protocol by Brian<br>
| + | |
| - | Move siRNA into our cryobox<br>
| + | |
| - | Grab sequencing primer from Jeremy's bench<br>
| + | |
| - | Pick colonies<br>
| + | |
| - | pEXPR: MAV1212-hEF1a-TAL14 (Mini)<br>
| + | |
| - | Give plates to Brian<br>
| + | |
| - | pENTR: TAL14p<br>
| + | |
| - | pENTR: TAL21p<br>
| + | |
| - | pENTR: Something for yeast for Brian<br>
| + | |
| - | Restriction Digest & run gel<br>
| + | |
| - | pEXPR: MAV1212-TRE-L7ae (LR)<br>
| + | |
| - | pEXPR: MAV1212-TRE-GAL4VP16 (LR)<br>
| + | |
| - | Transform<br>
| + | |
| - | pEXPR: MAV1212-hEF1a-GAL4VP16<br>
| + | |
| - | Golden Gate <br>
| + | |
| - | All the sensors<br>
| + | |
| - | Split and Seed 4 plates<br>
| + | |
| - | NOTES<br>
| + | |
| - | We transfected way too much DNA --> new protocol with 1 ug total<br>
| + | |
| - | <br>
| + | |
| - | 7.22<br>
| + | |
| - | Keep updating the parts tree (include experiments)<br>
| + | |
| - | Meet w/ Kyle<br>
| + | |
| - | FlowJo<br>
| + | |
| - | Bright Field Images<br>
| + | |
| - | miRNA Sensors (Theory)<br>
| + | |
| - | Troubleshoot<br>
| + | |
| - | What's going on with the double input low sensor<br>
| + | |
| - | Run new transfection plate/protocol by Brian<br>
| + | |
| - | Move siRNA into our cryobox<br>
| + | |
| - | Grab sequencing primer from Jeremy's bench<br>
| + | |
| - | Miniprep<br>
| + | |
| - | pEXPR: MAV1212-hEF1a-TAL14<br>
| + | |
| - | Restriction Digest & run gel (4 samples)<br>
| + | |
| - | pEXPR: MAV1212-TRE-L7ae (LR)<br>
| + | |
| - | Transform<br>
| + | |
| - | pEXPR: MAV1212-TRE-GAL4VP16 (LR)<br>
| + | |
| - | All the low sensors<br>
| + | |
| - | Transfect for L7ae Experiment 1.3<br>
| + | |
| - | <br>
| + | |
| - | <br>
| + | |
| - | NOTES<br>
| + | |
| - | We ran out of Lipofectamine and couldn't transfect <br>
| + | |
| - | <br>
| + | |
| - | 7.24<br>
| + | |
| - | How To Please Ron/Brain<br>
| + | |
| - | Keep updating the parts tree (include experiments)<br>
| + | |
| - | miRNA Sensors (Theory)<br>
| + | |
| - | miRNA Sensors (Lab Work)<br>
| + | |
| - | Midi prep<br>
| + | |
| - | pEXPR: MAV1212-hEF1a-TAL14<br>
| + | |
| - | Transform<br>
| + | |
| - | pEXPR: MAV1212-TRE-L7ae (LR from last night)<br>
| + | |
| - | Proteinase K<br>
| + | |
| - | MAV1212-hEF1a-TAL14<br>
| + | |
| - | Restriction Digest and run gel<br>
| + | |
| - | MAV1212-hEF1a-TAL14<br>
| + | |
| - | LR<br>
| + | |
| - | MAV1212-hEF1a-TAL21<br>
| + | |
| - | Mini prep<br>
| + | |
| - | Low Sensor miR-144<br>
| + | |
| - | Low Sensor miR-181c<br>
| + | |
| - | High Sensor miR-30d<br>
| + | |
| - | High Sensor miR-125b<br>
| + | |
| - | High Sensor miR-146a<br>
| + | |
| - | High Sensor let-7f<br>
| + | |
| - | Send for sequencing<br>
| + | |
| - | Low Sensor miR-144<br>
| + | |
| - | Low Sensor miR-181c<br>
| + | |
| - | High Sensor miR-30d<br>
| + | |
| - | High Sensor miR-125b<br>
| + | |
| - | High Sensor miR-146a<br>
| + | |
| - | High Sensor let-7f<br>
| + | |
| - | Pick colonies<br>
| + | |
| - | Low Sensor miR-144<br>
| + | |
| - | Low Sensor miR-181c<br>
| + | |
| - | High Sensor miR-30d<br>
| + | |
| - | High Sensor miR-125b<br>
| + | |
| - | High Sensor miR-146a<br>
| + | |
| - | High Sensor let-7f<br>
| + | |
| - | pEXPR: MAV1212-hEF1a-GAL4VP16<br>
| + | |
| - | pEXPR: MAV1212-TRE-GAL4VP16<br>
| + | |
| - | Seed plates<br>
| + | |
| - | L7ae experiment (Take 3)<br>
| + | |
| - | NOTES<br>
| + | |
| - | pEXPR: MAV1212-hEF1a-GAL4VP16 and pEXPR: MAV1212-TRE-GAL4VP16 only had blue colonies, which doesn't make sense because they were both verified...<br>
| + | |
| - | Brian is in the middle of something on his bench, so we won't midi today<br>
| + | |
| - | <br>
| + | |
| - | 7.25<br>
| + | |
| - | Keep updating the parts tree (include experiments)<br>
| + | |
| - | Troubleshoot<br>
| + | |
| - | What's the deal with the GAL4VP16?!<br>
| + | |
| - | Pick colonies<br>
| + | |
| - | pEXPR: MAV1212-TRE-L7ae (mini)<br>
| + | |
| - | MAV1212-hEF1a-GAL4VP16 (midi)<br>
| + | |
| - | MAV1212-TRE-GAL4VP16 (midi)<br>
| + | |
| - | Proteinase K<br>
| + | |
| - | MAV1212-hEF1a-TAL21<br>
| + | |
| - | MAV1212-hEF1a-TAL14<br>
| + | |
| - | Transform<br>
| + | |
| - | MAV1212-hEF1a-TAL21 (LR)<br>
| + | |
| - | MAV1212-hEF1a-TAL14 (LR)<br>
| + | |
| - | Plasmids from BCR group<br>
| + | |
| - | Plasmids from protein receptor group<br>
| + | |
| - | Plasmids from Brian<br>
| + | |
| - | Miniprep<br>
| + | |
| - | All the sensors (round 2)<br>
| + | |
| - | Send for sequencing<br>
| + | |
| - | All the sensors (round 2)<br>
| + | |
| - | Analyze sequencing results<br>
| + | |
| - | All the sensors (round 1)<br>
| + | |
| - | Transfect<br>
| + | |
| - | L7ae Experiment (Take 3)<br>
| + | |
| - | Seed<br>
| + | |
| - | TAL14 Experiment (Take 1)<br>
| + | |
| - | Re-anneal<br>
| + | |
| - | miR-144-181c double input target site<br>
| + | |
| - | Golden Gate<br>
| + | |
| - | Low Sensor miR-144-181c<br>
| + | |
| - | Inoculate midi cultures<br>
| + | |
| - | Each of the single input sensors<br>
| + | |
| - | NOTES<br>
| + | |
| - | All of the single input sensors were verified!<br>
| + | |
| - | 1st full day with no hiccups? <br>
| + | |
| - | Gave constructs and plasmids maps (Kturns, L7ae, and MAV RFP) to Jeremy<br>
| + | |
| - | 7.26<br>
| + | |
| - | Keep updating the parts tree (include experiments)<br>
| + | |
| - | Mini prep<br>
| + | |
| - | pEXPR: MAV1212-TRE-L7ae<br>
| + | |
| - | Digest and run gel<br>
| + | |
| - | pEXPR: MAV1212-TRE-L7ae<br>
| + | |
| - | Inoculate midi (if digest is okay)<br>
| + | |
| - | pEXPR: MAV1212-TRE-L7ae<br>
| + | |
| - | Midi prep<br>
| + | |
| - | MAV1212-hEF1a-GAL4VP16<br>
| + | |
| - | MAV1212-TRE-GAL4VP16<br>
| + | |
| - | All the single input sensors<br>
| + | |
| - | Pick colonies<br>
| + | |
| - | MAV1212-hEF1a-TAL21<br>
| + | |
| - | MAV1212-hEF1a-TAL14<br>
| + | |
| - | Plasmids from BCR group<br>
| + | |
| - | Plasmids from protein receptor group<br>
| + | |
| - | Plasmids from Brian<br>
| + | |
| - | Analyze sequencing results<br>
| + | |
| - | All the sensors (round 2)<br>
| + | |
| - | Transform<br>
| + | |
| - | Low Sensor miR-144-181c<br>
| + | |
| - | Transfect<br>
| + | |
| - | TAL14 Experiment (Take 1)<br>
| + | |
| - | Seed<br>
| + | |
| - | Experiment #3 (Take 1)<br>
| + | |
| - | Experiment #4 (Take 2)<br>
| + | |
| - | NOTES<br>
| + | |
| - | <br>
| + | |
| - | 7.28<br>
| + | |
| - | Keep updating the parts tree (include experiments)<br>
| + | |
| - | Include TAL14 and TAL21 high sensors<br>
| + | |
| - | Analyze sequencing results<br>
| + | |
| - | Plan DOX induction experiment for L7ae repression<br>
| + | |
| - | Plan to build TAL14/TAL21 high sensors<br>
| + | |
| - | Do gating and analysis of Exp 1 Attempt 3<br>
| + | |
| - | Mini prep<br>
| + | |
| - | pEXPR: MAV1212-TRE-L7ae<br>
| + | |
| - | Digest and run gel<br>
| + | |
| - | pEXPR: MAV1212-TRE-L7ae<br>
| + | |
| - | Inoculate midi (if digest is okay)<br>
| + | |
| - | pEXPR: MAV1212-TRE-L7ae<br>
| + | |
| - | Pick colonies<br>
| + | |
| - | MAV1212-hEF1a-TAL21<br>
| + | |
| - | MAV1212-hEF1a-TAL14<br>
| + | |
| - | Low Sensor miR-144-181c<br>
| + | |
| - | Golden Gate<br>
| + | |
| - | All the target sites & TAL14-mKate with Bbs1<br>
| + | |
| - | NOTES<br>
| + | |
| - | we're starting construction of the TAL14/21 high sensors<br>
| + | |
| - | <br> | + | |
| - | 7.29<br>
| + | |
| - | How To Please Ron/Brain<br>
| + | |
| - | Keep updating the parts tree (include experiments)<br>
| + | |
| - | Get TAL21 promoter from Brian<br>
| + | |
| - | Plan experiments<br>
| + | |
| - | DOX induction for L7ae repression (look here)<br>
| + | |
| - | Tuning TAL14 repression<br>
| + | |
| - | <br>
| + | |
| - | Learn about gating and FACS analysis<br>
| + | |
| - | Midi prep<br>
| + | |
| - | pEXPR: MAV1212-TRE-L7ae<br>
| + | |
| - | Mini prep<br>
| + | |
| - | MAV1212-hEF1a-TAL21<br>
| + | |
| - | MAV1212-hEF1a-TAL14<br>
| + | |
| - | Low Sensor miR-144-181c<br>
| + | |
| - | Sequence verify<br>
| + | |
| - | Low Sensor miR-144-181c<br>
| + | |
| - | Digest and run gel<br>
| + | |
| - | MAV1212-hEF1a-TAL21<br>
| + | |
| - | MAV1212-hEF1a-TAL14<br>
| + | |
| - | Golden Gate (if digest works)<br>
| + | |
| - | All the TAL21 high sensors<br>
| + | |
| - | Transform<br>
| + | |
| - | All the TAL14 high sensors<br>
| + | |
| - | And a plasmid from BCR<br>
| + | |
| - | NOTES<br>
| + | |
| - | The minipreps failed? re do tomorrow<br>
| + | |
| - | <br>
| + | |
| - | 7.30<br>
| + | |
| - | Keep updating the parts tree (include experiments)<br>
| + | |
| - | Run tuning experiments by Brian<br>
| + | |
| - | Get transformation code from Brian (hopefully)<br>
| + | |
| - | Midi prep<br>
| + | |
| - | pEXPR: TAL21-mKate<br>
| + | |
| - | Mini prep<br>
| + | |
| - | MAV1212-hEF1a-TAL21<br>
| + | |
| - | MAV1212-hEF1a-TAL14<br>
| + | |
| - | Low Sensor miR-144-181c<br>
| + | |
| - | Sequence verify<br>
| + | |
| - | Low Sensor miR-144-181c<br>
| + | |
| - | Digest and run gel<br>
| + | |
| - | MAV1212-hEF1a-TAL21<br>
| + | |
| - | MAV1212-hEF1a-TAL14<br>
| + | |
| - | Golden Gate (if digest works)<br>
| + | |
| - | All the TAL21 high sensors<br>
| + | |
| - | Pick colonies<br>
| + | |
| - | miR-30d (TAL14)<br>
| + | |
| - | miR-125b (TAL14)<br>
| + | |
| - | let-7f (TAL14)<br>
| + | |
| - | TAL14 mKate<br>
| + | |
| - | Inoculate midi culture (in case the digest works)<br>
| + | |
| - | TAL21<br>
| + | |
| - | NOTES<br>
| + | |
| - | The midi prep for TAL21-mKate didn't work --> pick a colony and try again tomorrow<br>
| + | |
| - | High sensor miR-146a didn't grow colonies --> retransform + replate transformation<br>
| + | |
| - | <br>
| + | |
| - | 7.31<br>
| + | |
| - | Keep updating the parts tree (include experiments)<br>
| + | |
| - | What about the double input low sensor? <br>
| + | |
| - | Midi prep<br>
| + | |
| - | pEXPR: TAL21-mKate<br>
| + | |
| - | Run gel<br>
| + | |
| - | MAV1212-hEF1a-TAL21<br>
| + | |
| - | MAV1212-hEF1a-TAL14<br>
| + | |
| - | Golden Gate (if digest works)<br>
| + | |
| - | All the TAL21 high sensors<br>
| + | |
| - | Gateway (LR)<br>
| + | |
| - | pEXPR: hEF1a-TAL21<br>
| + | |
| - | Pick colonies<br>
| + | |
| - | miR-30d (TAL14)<br>
| + | |
| - | miR-125b (TAL14)<br>
| + | |
| - | let-7f (TAL14)<br>
| + | |
| - | pEXPR: hEF1a-TAL21<br>
| + | |
| - | DOX Induction<br>
| + | |
| - | L7ae tuning experiment<br>
| + | |
| - | Transfect<br>
| + | |
| - | TAL14 tuning experiment<br>
| + | |
| - | NOTES<br>
| + | |
| - | hEF1a-TAL21 had a red pellet during midi prep spin down --> re-LR & re pick colonies<br>
| + | |
| - | <br>
| + | |
| - | 8.1<br>
| + | |
| - | Keep updating the parts tree (include experiments)<br>
| + | |
| - | Double input low sensor??<br>
| + | |
| - | Where are the siRNA?<br>
| + | |
| - | Mini prep<br>
| + | |
| - | miR-30d (TAL14)<br>
| + | |
| - | miR-16a (TAL14)<br>
| + | |
| - | let-7f (TAL14)<br>
| + | |
| - | pEXPR: hEF1a-TAL21<br>
| + | |
| - | Send for sequencing<br>
| + | |
| - | miR-30d (TAL14)<br>
| + | |
| - | miR-16a (TAL14)<br>
| + | |
| - | let-7f (TAL14)<br>
| + | |
| - | Digest and Run gel<br>
| + | |
| - | MAV1212-hEF1a-TAL21<br>
| + | |
| - | Flow Cytometry<br>
| + | |
| - | L7ae tuning experiment<br>
| + | |
| - | Transform <br>
| + | |
| - | miR-125b (TAL14)<br>
| + | |
| - | NOTES<br>
| + | |
| - | minipreps took a really long time --> TAL21 wasn't digested<br>
| + | |
| - | siRNA should be coming "soon"<br>
| + | |
| - | since HS_125b didn't grow colonies --> re transformed<br>
| + | |
| - | <br>
| + | |
| - | 8.4<br>
| + | |
| - | Keep updating the parts tree (include experiments)<br>
| + | |
| - | miRNA Sensors (Theory)<br>
| + | |
| - | Meet with Kyle about quantifying repression<br>
| + | |
| - | Troubleshoot gel results<br>
| + | |
| - | Digest and run gel<br>
| + | |
| - | MAV1212-hEF1a-TAL14<br>
| + | |
| - | MAV1212-hEF1a-TAL21<br>
| + | |
| - | LR (if digest fails)<br>
| + | |
| - | MAV1212-hEF1a-TAL21 - find pENTR TAL21<br>
| + | |
| - | Pick colonies (if digest failes)<br>
| + | |
| - | pENTR TAL14<br>
| + | |
| - | double input low sensor<br>
| + | |
| - | <br>
| + | |
| - | 8.7<br>
| + | |
| - | Keep updating the parts tree (include experiments)<br>
| + | |
| - | Meet with Kyle about quantifying repression<br>
| + | |
| - | Miniprep<br>
| + | |
| - | pENTR TAL14<br>
| + | |
| - | Double-Input Low Sensor<br>
| + | |
| - | Find<br>
| + | |
| - | pEXPR hEF1A:TAL14<br>
| + | |
| - | pENTR TAL21<br>
| + | |
| - | pEXPR hEF1A:TAL21<br>
| + | |
| - | LR <br>
| + | |
| - | MAV1212-hEF1a-TAL14<br>
| + | |
| - | MAV1212-hEF1a-TAL21<br>
| + | |
| - | Sequence<br>
| + | |
| - | Double-input low sensor<br>
| + | |
| - | Digest & run gel<br>
| + | |
| - | pENTR: TAL14<br>
| + | |
| - | Transfect<br>
| + | |
| - | L7ae quantification experiment<br>
| + | |
| - | NOTES<br>
| + | |
| - | <br>
| + | |
| - | 8.8<br>
| + | |
| - | Keep updating the parts tree (include experiments)<br>
| + | |
| - | Figure out MATLAB quantification<br>
| + | |
| - | Make plate maps for siRNA transfection<br>
| + | |
| - | Analyze Digest<br>
| + | |
| - | pENTR TAL14<br>
| + | |
| - | Analyze Sequencing Results<br>
| + | |
| - | Double-Input Low Sensor<br>
| + | |
| - | Add DOX<br>
| + | |
| - | L7ae quantification experiment<br>
| + | |
| - | LR <br>
| + | |
| - | hEF1a-TAL14 (if digest works)<br>
| + | |
| - | hEF1a-TAL21 (find entry vector / plate)<br>
| + | |
| - | NOTES<br>
| + | |
| - | <br>
| + | |
| - | 8.11<br>
| + | |
| - | Keep updating the parts tree (include experiments)<br>
| + | |
| - | FIND pENTR: TAL21 <br>
| + | |
| - | TASBE tools with Brian<br>
| + | |
| - | Anaylze sequencing results<br>
| + | |
| - | Pick up sensors from Jeremy<br>
| + | |
| - | Clean up experiments page<br>
| + | |
| - | Transform<br>
| + | |
| - | hEF1a-TAL14 (LR)<br>
| + | |
| - | Sensors from Jeremy? <br>
| + | |
| - | Inoculate midi culture<br>
| + | |
| - | Double input low sensor<br>
| + | |
| - | Tissue Culture<br>
| + | |
| - | Seed plate for miRNA sensors w/o siRNA<br>
| + | |
| - | Check dilutions in TC room<br>
| + | |
| - | NOTES<br>
| + | |
| - | <br>
| + | |
| - | 8.12<br>
| + | |
| - | Keep updating the parts tree (include experiments)<br>
| + | |
| - | FIND pENTR: TAL21 <br>
| + | |
| - | TASBE tools with Brian<br>
| + | |
| - | Pick up sensors from Jeremy<br>
| + | |
| - | Tissue Culture<br>
| + | |
| - | Seed plate for miRNA sensors w/ siRNA??<br>
| + | |
| - | Check dilutions in TC room<br>
| + | |
| - | NOTES<br>
| + | |
| - | <br>
| + | |
| - | 8.13<br>
| + | |
| - | Keep updating the parts tree (include experiments)<br>
| + | |
| - | Emal Jake Beal about TASBE tools<br>
| + | |
| - | Pick up sensors from Jeremy<br>
| + | |
| - | Tissue Culture<br>
| + | |
| - | Transfect plate for miRNA sensors w/ siRNA<br>
| + | |
| - | Miniprep<br>
| + | |
| - | hEF1a-TAL14<br>
| + | |
| - | (Restriction Digest and Run Gel)<br>
| + | |
| - | hEF1a-TAL14<br>
| + | |
| - | (Golden Gate)<br>
| + | |
| - | TAL14 High Sensors<br>
| + | |
| - | NOTES<br>
| + | |
| - | <br>
| + | |
| - | 8.14<br>
| + | |
| - | Keep updating the parts tree (include experiments)<br>
| + | |
| - | Emal Jake Beal about TASBE tools<br>
| + | |
| - | Pick up sensors from Jeremy<br>
| + | |
| - | And figure out what/when to do with them...<br>
| + | |
| - | New map for siRNA experiments<br>
| + | |
| - | Re-name siRNAs as "siRNA-[insert number here]"<br>
| + | |
| - | Figure out scatterplot in MATLAB<br>
| + | |
| - | Tissue Culture<br>
| + | |
| - | Cytometry for miRNA sensors w/o siRNA<br>
| + | |
| - | Golden Gate<br>
| + | |
| - | TAL14 High Sensors<br>
| + | |
| - | Grow in midi culture<br>
| + | |
| - | Double input low sensor<br>
| + | |
| - | GG Donor for TC<br>
| + | |
| - | hEF1a-TAL21<br>
| + | |
| - | <br>
| + | |
| - | 8.15<br>
| + | |
| - | Keep updating the parts tree (include experiments)<br>
| + | |
| - | Meet with Kyle about scatterplots in MATLAB<br>
| + | |
| - | Tissue Culture<br>
| + | |
| - | Cytometry for miRNA sensors with siRNA<br>
| + | |
| - | Digest verify<br>
| + | |
| - | MAV1212 hEF1a:Tal14<br>
| + | |
| - | Golden Gate<br>
| + | |
| - | TAL14 High Sensors<br>
| + | |
| - | Midi Prep<br>
| + | |
| - | Double input low sensor<br>
| + | |
| - | GG Donor for TC<br>
| + | |
| - | NOTES<br>
| + | |
| - | hEF1a-TAL14 is still giving us troubles (the gel doesn't quite make sense)
| + | |
| | </body> | | </body> |
| | </html> | | </html> |
"
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