Team:Sumbawagen

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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Sumbawagen&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<h1 class="firstHeading">Team:Sumbawagen/Team</h1>
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    <title>iGEM Sumbawagen 2014 &middot; Econey</title>
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            <a class="brand" href="https://2014.igem.org/Team:Sumbawagen">Sumbawagen</a>
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<a href="https://2014.igem.org/Team:Sumbawagen/Modeling"style="color:#000000"> Modeling</a></td>
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                <li class="active"><a href="https://2014.igem.org/Team:Sumbawagen">Home</a></li>
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                      <li><a href="https://2014.igem.org/Team:Sumbawagen/overviews/Econey_Project"> Econey Project </a></li>
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                    </ul>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/project/econey">Econey</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/project/result">Result</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/project/software">Software</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/parts">Parts</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Safety">Safety</a></li>  
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Attributions">Attribution</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/future_direction">Future Direction</a></li>                             
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                    <li class="nav-header">Daily Notes</li>
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                    <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook">Policy & Practices</a></li>
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                    <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook2">Lab</a></li>
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                    <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook/Protocol">Protocol</a></li>
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                    <li><a href="https://2014.igem.org/Team:Sumbawagen/Team">Meet the Team</a></li>
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                    <li><a href="https://2014.igem.org/Team:Sumbawagen/Team/Gallery">Gallery</a></li>
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                    <li><a href="https://2014.igem.org/Team:Sumbawagen/Team/Contact">Contact</a></li>
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                    <li class="nav-header">Social Media</li>
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                  <a href="#" class="dropdown-toggle" data-toggle="dropdown">Policy & Practices<!--<sup>*new</sup>--><b class="caret"></b></a>
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                    <li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/Policy">
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<strong>Policy & Practices</strong>
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<div style=" 
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/survey">Survey</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/Biotech">Biotech Camp</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/meet_the">Meet the farmers</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/meet">Meet US Ambassador</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/econey">@america</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/moyo">Moyo Festival</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/virtual">Virtual Outreach</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/Support">Responses and supports</a></li>
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                </li>
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                <li class="dropdown">
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-
<!--requirements section -->
 
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<p> Please be sure to keep these links, your audience will want to find your: </p>
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<!-- Links to other team pages -->
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              </ul>
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            </div><!--/.nav-collapse -->
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<li><a href="https://2014.igem.org/Team:Sumbawagen">Home</a> </li>
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<li><a href="https://igem.org/Team.cgi?year=2013&team_name=Sumbawagen">Official Team Profile</a> </li>
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+
-
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-
<li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook">Notebook</a> </li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Safety">Safety</a> </li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Attributions">Attributions</a> </li>
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<!-- Begin page content -->
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<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
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        <div class="span6" style="text-align:justify">
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<tr><td colspan="3" > <h3> Tips  </h3></td></tr>
+
        <h2 style="margin-top:-10px;"> Project Description </h2>
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        <p>
-
<td width="45%" valign="top">
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        BBa_J04450 has a lac promoter and mRFP gene, thus E. coli harboring this plasmid will give red color when the bacteria were grown with lactose or IPTG. Oppositely, the expression of mRFP will be decreased when glucose was present in medium through a mechanism called catabolite repression. We use this phenomenon to measure the concentration of glucose in honey by calibrating the color of E. coli medium using android-based mobile phone. Creation of novel circuit which consists of constitutive promoter followed by either Adenylate Cyclase or IIAGlc genes, which placed downward lac-mRFP circuit, may affect the sensitivity of catabolite repression. We expect an engineered E. coli, which shows red color expression in different range of glucose concentration. By changing mRFP gene with amilCP, a blue fluorescent protein, we may obtain E. coli expressing either red or blue color when different honey added to the medium due to different glucose concentration.<br><br></p>
-
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<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
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<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
 
-
<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
 
-
<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
 
-
<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
 
-
<li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
 
-
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-
<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
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-
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+
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-
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+
-->
-
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
+
 
-
<li>Have lots of fun! </li>
+
 
-
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+
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-
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+
      <div class="row">
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 +
 
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 +
              <span class="caption scale-caption" style="text-align:justify">
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                          <p><b style="font-size:16px;">ECONEY Project </b><br><br></p>
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 +
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 +
      </div>
 +
 
 +
<!-- Title -->
 +
      <div class="page-header">
 +
          <center><h2><b><strong>Acknowledgment<strong></b> </h2></center>
 +
<center><img src="https://static.igem.org/mediawiki/2014/7/75/BLKM.png"style="width:145px;height:225px;"/><br></center>
 +
 
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 +
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Latest revision as of 07:50, 19 February 2015

Team:Dundee/Team - 2013.igem.org

 

Team:Sumbawagen/Team

From 2014.igem.org

iGEM Sumbawagen 2014 · Econey

Sumbawagen

Project Description

BBa_J04450 has a lac promoter and mRFP gene, thus E. coli harboring this plasmid will give red color when the bacteria were grown with lactose or IPTG. Oppositely, the expression of mRFP will be decreased when glucose was present in medium through a mechanism called catabolite repression. We use this phenomenon to measure the concentration of glucose in honey by calibrating the color of E. coli medium using android-based mobile phone. Creation of novel circuit which consists of constitutive promoter followed by either Adenylate Cyclase or IIAGlc genes, which placed downward lac-mRFP circuit, may affect the sensitivity of catabolite repression. We expect an engineered E. coli, which shows red color expression in different range of glucose concentration. By changing mRFP gene with amilCP, a blue fluorescent protein, we may obtain E. coli expressing either red or blue color when different honey added to the medium due to different glucose concentration.


Acknowledgment


Sponsors



Retrieved from "https://2013.igem.org/Team:Dundee"

Retrieved from "https://2014.igem.org/Team:Sumbawagen"

Retrieved from "http://2014.igem.org/Team:Sumbawagen"