Team:UT-Dallas/Notebook/9-11
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<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr"> | <html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr"> | ||
+ | <!--navigation menu --> | ||
+ | <table align="center"> | ||
+ | <td align="center" colspan="12"> | ||
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+ | <table width="100%"> | ||
+ | <tr height="15px"></tr> | ||
+ | <tr height="75px"> | ||
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+ | <td style="display: inline; padding: 15px; text-decoration: none; " align="center" height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0> | ||
+ | <a href="https://2014.igem.org/Team:UT-Dallas"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;" >Home </a> </td> | ||
+ | |||
+ | <td style="display: inline; padding: 15px; text-decoration: none;" align="center" height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0> | ||
+ | <a href="https://2014.igem.org/Team:UT-Dallas/Team"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Team </a> </td> | ||
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+ | <td style="display: inline; padding: 15px; text-decoration: none;" align="center" height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0> | ||
+ | <a href="https://igem.org/Team.cgi?year=2014&team_name=UT-Dallas"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Official Profile </a></td> | ||
+ | |||
+ | <td style="display: inline; padding: 15px; text-decoration: none;" align="center" height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0> | ||
+ | <a href="https://2014.igem.org/Team:UT-Dallas/Project"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Project</a></td> | ||
+ | |||
+ | <td style="display: inline; padding: 15px; text-decoration: none;" align="center" height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0> | ||
+ | <a href="https://2014.igem.org/Team:UT-Dallas/Parts"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Parts</a></td> | ||
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+ | <td style="display: inline; padding: 15px; text-decoration: none;" align="center" height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0> | ||
+ | <a href="https://2014.igem.org/Team:UT-Dallas/Modeling"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Modeling</a></td> | ||
+ | |||
+ | <td style="display: inline; padding: 15px; text-decoration: none;" align="center" height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0> | ||
+ | <a href="https://2014.igem.org/Team:UT-Dallas/Notebook"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Notebook</a></td> | ||
+ | |||
+ | <td style="display: inline; padding: 15px; text-decoration: none;" align="center" height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0> | ||
+ | <a href="https://2014.igem.org/Team:UT-Dallas/Safety"style=" color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Safety </a></td> | ||
+ | |||
+ | <td style="display: inline; padding: 15px; text-decoration: none;" align="center" height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0> | ||
+ | <a href="https://2014.igem.org/Team:UT-Dallas/Attributions"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Attributions </a></td> | ||
+ | |||
+ | <td style="display: inline; padding: 15px; text-decoration: none;" align="center" height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0> | ||
+ | <a href="https://2014.igem.org/Team:UT-Dallas/Human-Practices"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Human Practices</a></td> | ||
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+ | |||
+ | <td style="display: inline; padding: 5px;" align ="center"> <a href="https://2014.igem.org/Team:UT-Dallas"> <img src="https://static.igem.org/mediawiki/2014/e/e1/UTDmodifiedlogo.jpg" height="45px"></a></td> | ||
+ | <td style="display: inline; padding: 5px;" align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td> | ||
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+ | </tr> | ||
+ | </table> | ||
+ | </tr> | ||
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+ | </tr> | ||
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+ | <tr> <td colspan="3" height="15px"> </td></tr> | ||
+ | <tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr> | ||
+ | <tr> <td colspan="3" height="5px"> </td></tr> | ||
+ | </table> | ||
+ | |||
+ | <!------end nav--> | ||
<h1 class="firstHeading" align="center">Thursday, September 11th, 2014</h1> | <h1 class="firstHeading" align="center">Thursday, September 11th, 2014</h1> | ||
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- | + | Made glycerol stock of clones inoculated yesterday. </p> | |
+ | <p> Test digested clones with SpeI and NheI (all clones are correct!). | ||
+ | <p> Start first batch of double transformations - inoculated competent cells with gRNA/Cas9 and corresponding reporter plasmids: acfA, acfB, ctxA, ctxB. | ||
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Latest revision as of 23:55, 17 October 2014
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Thursday, September 11th, 2014
Made glycerol stock of clones inoculated yesterday. Test digested clones with SpeI and NheI (all clones are correct!). Start first batch of double transformations - inoculated competent cells with gRNA/Cas9 and corresponding reporter plasmids: acfA, acfB, ctxA, ctxB. |