Team:Cambridge-JIC/Guide/Transformation

From 2014.igem.org

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=Transformation=
=Transformation=
This section contains the information for transforming Marchantia spores. The best established procedures take around 7 days.
This section contains the information for transforming Marchantia spores. The best established procedures take around 7 days.
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See the [[Team:Cambridge-JIC/Guide/Transformation/Outline outline]] for an overview of the process.
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See the [[Team:Cambridge-JIC/Guide/Transformation/Outline| outline]] for an overview of the process.
==Links==
==Links==
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The rough protocol is:
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The steps in transformation are roughly:
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1. Electroporate agrobacterium
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2. Prepare spores
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1. [[Team:Cambridge-JIC/Guide/Transformation/TransformingAgro | Electroporate Agrobacterium]]
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3. Cocultivate or use AgarTrap
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4. Plate transformants
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2. [[Team:Cambridge-JIC/Guide/Care/PreparingSpores | Prepare spores]]
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3. [[Team:Cambridge-JIC/Guide/Transformation/Cocultivation | Cocultivate]] or use [[Team:Cambridge-JIC/Guide/Transformation/AgarTrap| AgarTrap]]
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4. [[Team:Cambridge-JIC/Guide/Transformation/SelectingTransformants| Select transformants]]
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Latest revision as of 15:38, 17 October 2014

Cambridge iGEM 2014


Transformation

This section contains the information for transforming Marchantia spores. The best established procedures take around 7 days.

See the outline for an overview of the process.

Links

The steps in transformation are roughly:

1. Electroporate Agrobacterium

2. Prepare spores

3. Cocultivate or use AgarTrap

4. Select transformants