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Team:SYSU-China/file/Project/Notebook/Protocol/RNAT.html
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<h1>RNAT</h1> | <h1>RNAT</h1> | ||
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| + | <h2><b>Test Fluorescent</b></h2> | ||
| + | <p>1. Begin culture the strains</p> | ||
| + | <p>3mL three antibiotic LB culture per tube. </p> | ||
| + | <p>Overnight，then add Arabinose to induce</p> | ||
| + | <p>2. Prepare to test</p> | ||
| + | |||
| + | <p>Take 200μL from original culture to test OD</p> | ||
| + | <p>Discard the culture</p> | ||
| + | <p>Resuspend: OD=0.n add n*200 μL Yat-sen spring. (Because LB have very strong signal of green fluorescent)</p> | ||
| + | <p>3. Test fluorescent using the Hybrid reader in the fifth floor</p> | ||
| + | <p>Take 200μL from each tube to test OD and fluorescent. (3 wells repeat) </p> | ||
| + | <p>Test 3 times in the machine.</p> | ||
| + | |||
| + | <p><b>The parameters:</b></p> | ||
| + | <p>OD=600</p> | ||
| + | <p>Fluorescent: 485/528; 480/507; 510/550; 580/607</p> | ||
| + | |||
| + | <p>Repeat the whole experiment 3 times. </p> | ||
| + | |||
| + | |||
| + | |||
| + | <!--SYSUCHINA!--> | ||
Latest revision as of 02:04, 18 October 2014
RNAT
Test Fluorescent
1. Begin culture the strains
3mL three antibiotic LB culture per tube.
Overnight，then add Arabinose to induce
2. Prepare to test
Take 200μL from original culture to test OD
Discard the culture
Resuspend: OD=0.n add n*200 μL Yat-sen spring. (Because LB have very strong signal of green fluorescent)
3. Test fluorescent using the Hybrid reader in the fifth floor
Take 200μL from each tube to test OD and fluorescent. (3 wells repeat)
Test 3 times in the machine.
The parameters:
OD=600
Fluorescent: 485/528; 480/507; 510/550; 580/607
Repeat the whole experiment 3 times.
