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Team:Evry/Interlab Study/09-16-2014
From 2014.igem.org
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| - | <center>GEL | + | <center>GEL 1: 1% agarose gel. PCR product of 5 colonies of the PSB1C3+E0240+J23116 and PSB1C3+E0240+J23115 constructions.</center> |
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| + | A pre-culture was made with one colony for each construction to make a glycerol stock.<br> | ||
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| + | <i>PSB1C3+E0240+J23117</i> - <i>PSB1C3+E0240+J23119</i>: | ||
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| + | <li> PCR colony of 6 colonies for each construction following protocol table 1 and 2. | ||
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<div class="thumbinner" style="width:502px;"> | <div class="thumbinner" style="width:502px;"> | ||
| - | <a href="https://static.igem.org/mediawiki/2014/ | + | <a href="https://static.igem.org/mediawiki/2014/d/d5/117-119.jpg" class="image"> |
| - | <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/ | + | <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/d/d5/117-119.jpg" width="450px;" class="thumbimage"/> |
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| - | <a href="https://static.igem.org/mediawiki/2014/ | + | <a href="https://static.igem.org/mediawiki/2014/d/d5/117-119.jpg" class="internal" title="Enlarge"> |
<img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="Symbol"/> | <img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="Symbol"/> | ||
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| - | <center>GEL 2: 1% agarose gel. PCR product of | + | <center>GEL 2: 1% agarose gel. PCR product of 6 colonies of the PSB1C3+E0240+J23117 and PSB1C3+E0240+J23119 constructions.</center> |
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| + | A pre-culture was made with one colony for each construction to make a glycerol stock.<br> | ||
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Latest revision as of 18:59, 17 October 2014
Construction n°1: PSB1C3 with I20260
Construction n°2: PSB1C3 with J23101-E1010
Construction n°3: PSB1C3 with K823012-E1010
Other constructions of the Anderson library of constitutive promoters
PSB1C3+E0240+J23100:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23101:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23102:
- glycerol stock preparation from LB culture from the 14th September.
- Measure of the cells' growth and fluorecence on TECAN infiniteM200.(18h30-1h30):
PSB1C3+E0240+J23106:
- glycerol stock preparation from LB culture from the 14th September.
- Measure of the cells' growth and fluorecence on TECAN infiniteM200.(2h-9h):
PSB1C3+E0240+J23115 - PSB1C3+E0240+J23115:
- PCR colony of 5 colonies for each construction following protocol table 1 and 2.
GEL 1: 1% agarose gel. PCR product of 5 colonies of the PSB1C3+E0240+J23116 and PSB1C3+E0240+J23115 constructions.
A pre-culture was made with one colony for each construction to make a glycerol stock.
PSB1C3+E0240+J23117 - PSB1C3+E0240+J23119:
- PCR colony of 6 colonies for each construction following protocol table 1 and 2.
GEL 2: 1% agarose gel. PCR product of 6 colonies of the PSB1C3+E0240+J23117 and PSB1C3+E0240+J23119 constructions.
A pre-culture was made with one colony for each construction to make a glycerol stock.
Sep 16
