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Team:Evry/Interlab Study/09-18-2014
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Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C. | Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C. | ||
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<li> PCR colony of 5 colonies for each construction following protocol table 1 and 2. | <li> PCR colony of 5 colonies for each construction following protocol table 1 and 2. | ||
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| - | <center>GEL | + | <center>GEL 1: 1% agarose gel. PCR product of 5 colonies of the PSB1C3+E0240+J23114 construction. </center> |
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| - | + | A pre-culture was made with one colony to make a glycerol stock.<br> | |
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Latest revision as of 18:59, 17 October 2014
Construction n°1: PSB1C3 with I20260
Construction n°2: PSB1C3 with J23101-E1010
Construction n°3: PSB1C3 with K823012-E1010
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (00h-07h).
Other constructions of the Anderson library of constitutive promoters
PSB1C3+E0240+J23101:
PSB1C3+E0240+J23102:
PSB1C3+E0240+J23104:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23105:
glycerol stock preparation from LB culture of the 17th September.
PSB1C3+E0240+J23107:
glycerol stock preparation from LB culture of the 17th September.
PSB1C3+E0240+J23110:
Pre-culture does'nt grow. The TECAN measure is reported.
PSB1C3+E0240+J23115:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23116:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23117:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23114:
- PCR colony of 5 colonies for each construction following protocol table 1 and 2.
GEL 1: 1% agarose gel. PCR product of 5 colonies of the PSB1C3+E0240+J23114 construction.
A pre-culture was made with one colony to make a glycerol stock.
Sep 18
