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Team:Peking/secondtry/Parts
From 2014.igem.org
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<h2>Parts</h2> | <h2>Parts</h2> | ||
<h3 id="parts01">Favorite Parts</h3> | <h3 id="parts01">Favorite Parts</h3> | ||
| - | <p>INPNC-MVN-mRFP</p> | + | <p><b>INPNC-MVN-mRFP</b></p> |
<p><a href="http://partsregistry.org/Part:BBa_K1378005">Main Page-BBa_K1378005</a> | <p><a href="http://partsregistry.org/Part:BBa_K1378005">Main Page-BBa_K1378005</a> | ||
<p>BBa_K1378005 is a composite part coding INPNC-MVN-mRFP fusion protein. We confirmed that fusion protein is truly displayed outwards by using the INPNC displaying system by rTEV protease digestion assay method and thus help characterize INPNC part.</p> | <p>BBa_K1378005 is a composite part coding INPNC-MVN-mRFP fusion protein. We confirmed that fusion protein is truly displayed outwards by using the INPNC displaying system by rTEV protease digestion assay method and thus help characterize INPNC part.</p> | ||
| - | <p>Visit Wiki</ | + | <p><a href="https://2014.igem.org/Team:Peking/secondtry/KillingImprovements">Visit Wiki</a> |
| - | <p>MlrA</p> | + | <p><b>MlrA</b></p> |
| - | <p><a href="http://partsregistry.org/Part: | + | <p><a href="http://partsregistry.org/Part:BBa_K1378001">Main Page-BBa_K1378001</a> |
<p>MlrA is a protease which can cleavage Microcystin(MC) and and thus reduce its toxicity. We tested it in E.coli and showed that MlrA can decrease the concentration of MC and weaken its inhibitory activity to Protein Phosphatase 1.</p> | <p>MlrA is a protease which can cleavage Microcystin(MC) and and thus reduce its toxicity. We tested it in E.coli and showed that MlrA can decrease the concentration of MC and weaken its inhibitory activity to Protein Phosphatase 1.</p> | ||
| - | <p>Visit Wiki</p> | + | <p><a href="https://2014.igem.org/Team:Peking/secondtry/Degradation">Visit Wiki</a> |
| - | + | <p><b>INPNC-MVN & GFP</b></p> | |
| + | <p><a href="http://partsregistry.org/Part:BBa_K1378007">Main Page-BBa_K1378007</a> | ||
| + | <p>BBa_K1378007 is a composite part coding INPNC-MVN fusion protein and GFP. E coli carrying this plasmid will display MVN on the cell surface and bind to M. aeruginosa as well as expression GFP, thus the binding phenomenon can be easily observed.</p> | ||
| + | <p><a href="https://2014.igem.org/Team:Peking/secondtry/KillingImprovements">Visit Wiki</a> | ||
<h3 id="parts02">Part List</h3> | <h3 id="parts02">Part List</h3> | ||
Latest revision as of 04:02, 17 October 2014
Parts
Favorite Parts
INPNC-MVN-mRFP
BBa_K1378005 is a composite part coding INPNC-MVN-mRFP fusion protein. We confirmed that fusion protein is truly displayed outwards by using the INPNC displaying system by rTEV protease digestion assay method and thus help characterize INPNC part.
MlrA
MlrA is a protease which can cleavage Microcystin(MC) and and thus reduce its toxicity. We tested it in E.coli and showed that MlrA can decrease the concentration of MC and weaken its inhibitory activity to Protein Phosphatase 1.
INPNC-MVN & GFP
BBa_K1378007 is a composite part coding INPNC-MVN fusion protein and GFP. E coli carrying this plasmid will display MVN on the cell surface and bind to M. aeruginosa as well as expression GFP, thus the binding phenomenon can be easily observed.
Part List
Parts for Killing Improvement
BBa_K1378003 is the coding sequence of lectin Microvirin.
BBa_K1378005 is the coding sequence of INPNC-MVN-mRFP fusion protein.
BBa_K1378007 is a composite part of GFP and INPNC-MVN fusion protein.
BBa_K1378009 is a composite part of GFP and INPNC-MVN fusion protein with a linker between INPUC and MVN which can be cleavaged by rTEV.
Parts for Degradation
BBa_K1378001 is protease MlrA which can specifically cleavage Microcystin(MC).
BBa_K1378022 is the coding sequence of MlrA fused with PelB secretion tag.
BBa_K1378023 is the coding sequence of MlrA fused with TorA secretion tag.
BBa_K1378024 is the coding sequence of MlrA with terminator.
BBa_K1378025 is the coding sequence of PelB-MlrA fusion protein with terminator.
BBa_K1378026 is the coding sequence of TorA-MlrA fusion protein with terminator.
Parts for Suicide
BBa_K1378031 is the coding sequence for holin.
BBa_K1378032 is the coding sequence for endolysin.
