Guide/Materials/Strains

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Marchantia
 
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The Haseloff lab uses the CAM1 and CAM2 strain. Other researched strains are the TAK1 strain.
 
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To create your own stock of marchantia, you can also use a local strain. Marchantia can be found widely in damp areas, or in plant nurseries as a weed. Cultivate these in soil - they require very little attention - and follow [[sexual reproduction]] to gain your own spores.
 
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Agrobacterium
 
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GV2260 works, and other strains probably do also.
 
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You can either purchase these directly, or get a stab sent to you by any plant lab.
 
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You will need to [[prepare electrocompetent agro]] in order to use them for plant transformation.
 
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E-Coli
 
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Use any strain you have access to! They just need to be able to grow microgram quantities of plasmid DNA!
 

Latest revision as of 21:16, 16 October 2014