Team:Gaston Day School/Parts

From 2014.igem.org

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<br>In 2012 our team created a cadmium detector part K824008. We also created several other heavy metal detectors, but since our team was unable to gain access to other heavy metals due to our BS1 status, and cadmium was quickly becoming a local issue, we decided to focus on improving our cadmium detector. This first detector was sensitive to 10 mM of cadmium. This year, with the addition of sensitivity tuners, we improved the sensitivity four-fold. We can now detect 2.5 mM of cadmium, which is a much more realistic and useful concentration to find. At 2.5 mM of cadmium, the presence is not necessarily apparent in water, but is still above safe levels and the federal limit.
<br>In 2012 our team created a cadmium detector part K824008. We also created several other heavy metal detectors, but since our team was unable to gain access to other heavy metals due to our BS1 status, and cadmium was quickly becoming a local issue, we decided to focus on improving our cadmium detector. This first detector was sensitive to 10 mM of cadmium. This year, with the addition of sensitivity tuners, we improved the sensitivity four-fold. We can now detect 2.5 mM of cadmium, which is a much more realistic and useful concentration to find. At 2.5 mM of cadmium, the presence is not necessarily apparent in water, but is still above safe levels and the federal limit.
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<br>2012 Cd Detector Results
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<br><img src="https://static.igem.org/mediawiki/2014/2/21/2012_Cd_graph.jpg" alt="6.11.14iso.png" style="width:100%">
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<br>
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<br>2014 Cd Detector Results
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<br><img src="https://static.igem.org/mediawiki/2014/d/db/Cadmium_graph.jpg" alt="6.11.14iso.png" style="width:100%">
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                         K1320100
                         K1320100
                         </a>
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<br> Gel showing our working GlmY construct. Wells are as follows:
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<br>1.Ladder
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<br>2.Control
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<br>3.GlmY
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<br>4.GlmY
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<br><img src="https://static.igem.org/mediawiki/2014/thumb/3/39/Working_GlmY_Gel.jpg/336px-Working_GlmY_Gel.jpg" alt="6.11.14iso.png" style="width:100%">
                     </div>
                     </div>
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Latest revision as of 21:23, 17 October 2014

2014 GDS iGEM Parts Page

>Parts

K1320000

In 2012 our team created a cadmium detector part K824008. We also created several other heavy metal detectors, but since our team was unable to gain access to other heavy metals due to our BS1 status, and cadmium was quickly becoming a local issue, we decided to focus on improving our cadmium detector. This first detector was sensitive to 10 mM of cadmium. This year, with the addition of sensitivity tuners, we improved the sensitivity four-fold. We can now detect 2.5 mM of cadmium, which is a much more realistic and useful concentration to find. At 2.5 mM of cadmium, the presence is not necessarily apparent in water, but is still above safe levels and the federal limit.

2012 Cd Detector Results
6.11.14iso.png

2014 Cd Detector Results
6.11.14iso.png
K1320100

Gel showing our working GlmY construct. Wells are as follows:
1.Ladder
2.Control
3.GlmY
4.GlmY
6.11.14iso.png

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