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Team:Groningen/Template/MODULE/Notebook/toolbox/week2
From 2014.igem.org
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We decided to use the CP promoter collection and test it in | We decided to use the CP promoter collection and test it in | ||
<i>Lactococcus lactis</i>, that was BioBricked by | <i>Lactococcus lactis</i>, that was BioBricked by | ||
| - | the Uppsala iGEM team of 2013. The CP promoters are registered as | + | the Uppsala iGEM team of 2013. The CP promoters are registered as <a href="http://parts.igem.org/Part:BBa_K1033219"> CP1</a>, <a href="http://parts.igem.org/Part:BBa_K1033220">CP8</a>, <a href="http://parts.igem.org/Part:BBa_K1033221">CP11</a>, <a href="http://parts.igem.org/Part:BBa_K1033222">CP29</a>, |
| - | BBa_K1033219 | + | <a href="http://parts.igem.org/Part:BBa_K1033223">CP30</a>, <a href="http://parts.igem.org/Part:BBa_K1033224">CP41</a> and <a href="http://parts.igem.org/Part:BBa_K1033225">CP44</a>. These parts were |
| - | BBa_K1033223 | + | |
ordered from iGEM HQ, except for CP8 as for this promoter the sequencing | ordered from iGEM HQ, except for CP8 as for this promoter the sequencing | ||
was inconsistent. The promoters were sent by iGEM HQ using | was inconsistent. The promoters were sent by iGEM HQ using | ||
Latest revision as of 01:19, 18 October 2014
14 July - 20 July
A collection of constitutive promoters was also desirable for the toolbox.
We decided to use the CP promoter collection and test it in
Lactococcus lactis, that was BioBricked by
the Uppsala iGEM team of 2013. The CP promoters are registered as CP1, CP8, CP11, CP29,
CP30, CP41 and CP44. These parts were
ordered from iGEM HQ, except for CP8 as for this promoter the sequencing
was inconsistent. The promoters were sent by iGEM HQ using
Escherichia coli containing pSB1C3 with the promoter as insert.
E. coli was grown and the plasmids with the promoter were
isolated.
