|
|
| (46 intermediate revisions not shown) |
| Line 1: |
Line 1: |
| - | {{Template:Team:DTU-Denmark/maintemplate}}
| |
| | | | |
| - | <html>
| |
| - |
| |
| - | <body>
| |
| - | <div class="pagescontent" style="top:50px; left:50px; width:600px; background-image:url('https://static.igem.org/mediawiki/2014/d/d5/DTU-Denmark-FrameCalV1.png'); background-size: 50%; background-repeat: no-repeat; z-index:30;">
| |
| - | <maincontent>
| |
| - |
| |
| - |
| |
| - | <form onchange = "
| |
| - | if (fluorcheck.checked) { concentration.value = (parseFloat(fluorescence.value)-parseFloat(intercept.value)) / parseFloat(slope.value) };
| |
| - | if (CFUcheck.checked) { OD600.value = (parseFloat(CFU.value)*10000000000000) };
| |
| - | if (degcheck.checked) { degen.value = Math.log(2)/(parseFloat(deghalf.value)*parseFloat(deghalfunit.value)) };
| |
| - | if (growthcheck.checked) { growth.value = Math.log(2)/(parseFloat(grodouble.value)*parseFloat(grodoubleunit.value)) };
| |
| - | activity.value = (parseFloat(concentration.value)/parseFloat(OD600.value))*(602200/(parseFloat(foldfraction.value)/100))*((parseFloat(degen.value)/parseFloat(degenunit.value))+(parseFloat(growth.value)/parseFloat(growthunit.value)))/parseFloat(copynumber.value);
| |
| - |
| |
| - | activity.value = Math.round(parseFloat(activity.value)*1000)/1000;
| |
| - |
| |
| - | if (activity.value >= 1000) { activity.value = activity.value.toExponential(); }
| |
| - |
| |
| - | ">
| |
| - | <div id="PoPScalculator">
| |
| - | <center>
| |
| - | <table width="600px" border="0" cellspacing="5" cellpadding="0" style="background: transparent;">
| |
| - | <tr colspan="6">
| |
| - | Promoter activity calculator
| |
| - | </tr>
| |
| - |
| |
| - | <tr>
| |
| - |
| |
| - | <td width="165px" colspan="2">
| |
| - | Fluorescence:
| |
| - | </td>
| |
| - | <td style="text-align:right; padding-top: 4px;">
| |
| - | <input name="fluorescence" type="number" step="any" min="0" placeholder="Enter number"style="width:80px" />
| |
| - | </td>
| |
| - | <td></td>
| |
| - |
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td colspan="2">
| |
| - | Standard curve, slope:
| |
| - | </td>
| |
| - | <td style="text-align:right; padding-top: 4px;">
| |
| - | <input name="slope" type="number" step="any" min="0" placeholder="slope" style="width:80px" />
| |
| - | </td>
| |
| - | <td></td>
| |
| - | </tr>
| |
| - |
| |
| - | <tr>
| |
| - | <td colspan="2">
| |
| - | Standard curve, intercept:
| |
| - | </td>
| |
| - | <td style="text-align:right; padding-top: 4px;">
| |
| - | <input name="intercept" type="number" step="any" min="0" placeholder="intercept" style="width:80px" />
| |
| - | </td>
| |
| - | <td></td>
| |
| - | </tr>
| |
| - |
| |
| - | <tr>
| |
| - | <td colspan="2">
| |
| - | Spinach concentration:
| |
| - | </td>
| |
| - | <td style="text-align:right; padding-top: 4px;" width=80px">
| |
| - | <input name="concentration" type="number" step="any" min="0" placeholder="Enter number" value="0.45" style="width:80px" />
| |
| - | <td>µM</td>
| |
| - | </tr>
| |
| - |
| |
| - | <tr>
| |
| - | <td colspan="2">OD600</td>
| |
| - | <td style="text-align:right; padding-top: 4px;">
| |
| - | <input name="OD600" type="number" step="any" min="0" placeholder="Enter number" value="6.3" style="width:80px" />
| |
| - | </td>
| |
| - | <td></td>
| |
| - | <td>CFU count</td>
| |
| - | <td style="text-align:right; padding-top: 4px;">
| |
| - | <input name="CFU" type="number" step="any" min="0" placeholder="Count" style="width:80px" />
| |
| - | </td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td style="padding-top:7px"><input type="checkbox" name="CFUcheck"></td><td>Use CFU count</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td colspan="2">Degradation rate</td>
| |
| - | <td style="text-align:right; padding-top: 4px;">
| |
| - | <input name="degen" type="number" step="any" min="0" placeholder="Enter number" value="0.083" style="width:80px" />
| |
| - | </td>
| |
| - | <td style="padding-top:4px">
| |
| - | <select name="degenunit">
| |
| - | <option value="3600">h-1</option>
| |
| - | <option value="60">m-1</option>
| |
| - | <option value="1">s-1</option>
| |
| - | </select>
| |
| - | </td>
| |
| - | <td>Degradation half time</td>
| |
| - | <td style="text-align:right; padding-top: 4px;">
| |
| - | <input name="deghalf" type="number" step="any" min="0" placeholder="Half time" style="width:80px" />
| |
| - | </td>
| |
| - | <td style="padding-top:4px">
| |
| - | <select name="deghalfunit">
| |
| - | <option value="3600">h</option>
| |
| - | <option value="60">m</option>
| |
| - | <option value="1">s</option>
| |
| - | </select>
| |
| - | </td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td style="padding-top:7px"><input type="checkbox" name="degcheck"></td><td>Use degradation half time</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td colspan="2">Growth rate</td>
| |
| - | <td style="text-align:right; padding-top: 4px;">
| |
| - | <input name="growth" type="number" step="any" min="0" placeholder="Doubling time" value="0.83" style="width:80px" />
| |
| - | <td style="padding-top:4px">
| |
| - | <select name="growthunit">
| |
| - | <option value="3600">h-1</option>
| |
| - | <option value="60">m-1</option>
| |
| - | <option value="1">s-1</option>
| |
| - | </select>
| |
| - | </td>
| |
| - | <td>Growth doubling time</td>
| |
| - | <td style="text-align:right; padding-top: 4px;">
| |
| - | <input name="grodouble" type="number" step="any" min="0" placeholder="Doubling time" style="width:80px" />
| |
| - | <td style="padding-top:4px">
| |
| - | <select name="grodoubleunit">
| |
| - | <option value="3600">h</option>
| |
| - | <option value="60">m</option>
| |
| - | <option value="1">s</option>
| |
| - | </select>
| |
| - | </td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td style="padding-top:7px"><input type="checkbox" name="growthcheck"></td><td>Use growth doubling time<br></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td colspan="2">Fold-fraction</td>
| |
| - | <td style="text-align:right; padding-top: 4px;">
| |
| - | <input name="foldfraction" type="number" step="any" min="0" placeholder="Enter number" value="50" style="width:80px" />
| |
| - | </td>
| |
| - | <td>%</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td colspan="2">Copy number</td>
| |
| - | <td style="text-align:right; padding-top: 4px;">
| |
| - | <input name="copynumber" type="number" step="any" min="0" placeholder="Enter number" value="300" style="width:80px" />
| |
| - | <td></td>
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - |
| |
| - | </center>
| |
| - | </div>
| |
| - |
| |
| - |
| |
| - | </maincontent>
| |
| - | </div>
| |
| - | </body>
| |
| - |
| |
| - |
| |
| - | </html>
| |
| - | {{:Template:Team:DTU-Denmark/footer}}
| |
"
