Team:WashU StLouis/Project/collaboration

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<p>Collaboration with Penn State & anyone else</p>
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<p> We also collaborated with the University of Virginia's
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iGEM Team by helping them distribute their survey! For more
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information, contact them at virginia.igem@gmail.com </p>
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<div style="text-align: center;"><br>
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We also helped to characterize a few parts from the registry:<br>
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<p style="text-align: justify;"><a
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href="http://parts.igem.org/Part:BBa_M30109:Experience#User_Reviews">BBa_M30109</a>
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<br>
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BBa_M30109 does NOT work AT ALL. Digesting the plasmid with the iGEM
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restriction enzyme yields the right band lengths but other than that
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the sequence is junk. We tried on numerous occasions to assemble a
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plasmid to activate our light sensor system. This set us back half of
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the summer because of the faulty part. We found a new part on the
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registry with the same genes that showed slightly more promise.&nbsp;</p>
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<p style="text-align: justify;"><a
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href="http://parts.igem.org/Part:BBa_K1017726:Experience#User_Reviews">BBa_K1017726</a>
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<br>
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BBa_K1017726 digests with the iGEM restriction enzymes well. We were
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able to assemble a plasmid by swapping out ori and resistance (we
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needed a different antibiotic) in order to co-transform with our
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system. There is differentiable results between our light and dark
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system, so the part likely has the genes as reported. However,
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digestion with known restriction enzyme sites based off the sequence
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provided gave gel smears rather than clear bands, so not 100% convinced
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that this part is as advertised. </p>
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Latest revision as of 21:39, 17 October 2014



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