Team:INSA-Lyon/Parts

From 2014.igem.org

(Difference between revisions)
 
(6 intermediate revisions not shown)
Line 12: Line 12:
<div id="icones">
<div id="icones">
<ul>
<ul>
-
<a href="https://2014.igem.org/Team:INSA-Lyon/Biology" class="hu-icon"><li class="iconmulti"  style="line-height:20px;  ">WETLAB SUMMARY</li></a>
+
<a href="https://2014.igem.org/Team:INSA-Lyon/Biology" class="hu-icon"><li class="iconmulti">WETLAB SUMMARY</li></a>
-
<a href="https://2014.igem.org/Team:INSA-Lyon/Results" class="hu-icon"><li class="iconmulti"  style="line-height:40px;  ">RESULTS</li></a>
+
<a href="https://2014.igem.org/Team:INSA-Lyon/Results" class="hu-icon"><li class="icon">RESULTS</li></a>
-
<a href="https://2014.igem.org/Team:INSA-Lyon/Notebook" class="hu-icon"><li class="iconmulti"  style="line-height:40px;  ">NOTEBOOK</li></a>
+
<a href="https://2014.igem.org/Team:INSA-Lyon/Notebook" class="hu-icon"><li class="icon">NOTEBOOK</li></a>
-
<a href="https://2014.igem.org/Team:INSA-Lyon/Parts" class="hu-icon"><li class="iconmulti"  style="line-height:40px;  ">PARTS</li></a>
+
<a href="https://2014.igem.org/Team:INSA-Lyon/Protocol" class="hu-icon"><li class="icon">PROTOCOLS</li></a>
-
<a href="https://2014.igem.org/Team:INSA-Lyon/DataPage" class="hu-icon"><li class="iconmulti"  style="line-height:40px;  ">DATA PAGE</li></a>
+
<a href="https://2014.igem.org/Team:INSA-Lyon/DataPage" class="hu-icon"><li class="icon">DATA PAGE</li></a>
</ul>
</ul>
</div>
</div>
Line 28: Line 28:
<li><div align="justify">
<li><div align="justify">
-
<p>5 biobricks have been constructed, three for Nickel chelation, three for promoter characterization.</p></br>
+
<p>5 biobricks have been constructed, thre for Nickel chelation, two for promoter characterization.</p></br>
<b>Biobricks for nickel chelation</b></br></br>
<b>Biobricks for nickel chelation</b></br></br>
Line 37: Line 37:
<li><div align="justify">
<li><div align="justify">
<p>On a pSB1C3 backbone, two essential part have been assembled: a promoter and a reporter coding for CsgA.The promoter in this case is always the same: P70.However, the reporter is different for each construction.</br>
<p>On a pSB1C3 backbone, two essential part have been assembled: a promoter and a reporter coding for CsgA.The promoter in this case is always the same: P70.However, the reporter is different for each construction.</br>
-
The first CsgA is called CsgA WT, the sequence is exactly the same as we found in a wild type CsgA protein except one mutation in order to eliminate one restriction site. The construction is also called CsgA.</br>
+
The first CsgA is called CsgA WT, the sequence is exactly the same as we found in a wild type CsgA protein except one mutation in order to eliminate one restriction site. The construction is also called CsgA-WT.</br>
-
The second CsgA is based on the same sequence but with a short sequence coding for a his tag(a peptide chain coding six histidines). This construction is called His1.</br>
+
The second CsgA is based on the same sequence but with a short sequence coding for a his tag(a peptide chain coding six histidines). This construction is called CsgA-His1.</br>
-
The last CsgA is almost the same but with 2 his tag. This construction is called His2.</br>
+
The last CsgA is almost the same but with 2 his tag. This construction is called CsgA-His2.</br>
-
5 biobricks have been constructed, three for Nickel chelation, three for promoter characterization.</br>
 
-
<b>Biobrick for promoter characterization</b></br>
 
-
The principal of the construction is shown in the figure below.</br></div>
 
-
<li><div align="center">
 
-
<img src= "https://static.igem.org/mediawiki/parts/f/fb/2.png"></br></div>
 
-
<li><div align="justify">
 
-
<p>On a pKK backbone, two essential parts have been assembled: a promoter and a reporter.</br>
 
-
The reporter in this case is always the same: gfp. However, the promoter is different for each construction.</br>
 
-
The first promoter is P70, sequence found in the Resgistry. This construction is called p70::gfp</br>
 
-
The second promoter is PCurli, obtained from a PCR, sequence coding for the inter-genic regulation for curli production. This construction is called pcurli::gfp.</p></br></div>
 
</div></li>
</div></li>

Latest revision as of 17:07, 17 October 2014

Curly'on - IGEM 2014 INSA-LYON

  • 5 biobricks have been constructed, thre for Nickel chelation, two for promoter characterization.


    Biobricks for nickel chelation

    The principal of the construction is shown in the figure below.





  • On a pSB1C3 backbone, two essential part have been assembled: a promoter and a reporter coding for CsgA.The promoter in this case is always the same: P70.However, the reporter is different for each construction.
    The first CsgA is called CsgA WT, the sequence is exactly the same as we found in a wild type CsgA protein except one mutation in order to eliminate one restriction site. The construction is also called CsgA-WT.
    The second CsgA is based on the same sequence but with a short sequence coding for a his tag(a peptide chain coding six histidines). This construction is called CsgA-His1.
    The last CsgA is almost the same but with 2 his tag. This construction is called CsgA-His2.

    • <groupparts>iGEM014 INSA-Lyon</groupparts>

    Retrieved from "http://2014.igem.org/Team:INSA-Lyon/Parts"