Team:UCL/Tests

From 2014.igem.org

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   <img src="https://static.igem.org/mediawiki/2014/2/21/OExperiments_Bannero.jpg" width="100%" height="100%" alt="Experiments" />
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   <img src="https://static.igem.org/mediawiki/2014/0/07/OAchievements_Bannero.jpg" width="100%" height="100%" alt="Achievements" />
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<ul class="tabs">
<ul class="tabs">
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     <li><a href="#view1">Stage 1</a></li>
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     <li><a href="#view1">Bronze</a></li>
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     <li><a href="#view2">Stage 2</a></li>
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     <li><a href="#view2">Silver</a></li>
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     <li><a href="#view3">Stage 3</a></li>
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     <li><a href="#view3">Gold</a></li>
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     <li><a href="#view4">Stage 4</a></li>
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     <li><a href="#view4">Special Prizes</a></li>
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    <li><a href="#view5">Stage 5</a></li>
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    <li><a href="#view6">Stage 6</a></li>
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    <li><a href="#view7">Stage 7</a></li>
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    <li><a href="#view8">Stage 8</a></li>
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</ul>
</ul>
<div class="tabcontents">
<div class="tabcontents">
<!--- This is the overview section --->
<!--- This is the overview section --->
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<div id="view1"><div class="textTitle"><h4>Stage 01: Extraction of useful BioBrick plasmids from iGEM 2014 Distribution Kit</h4></div><br>
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<div id="view1"><div class="textTitle"><h4>Bronze Achieved!</h4></div><br>
<!-- <a data-tip="true" class="top large" data-tip-content="Here's Tanel doing some pipetting in our lab!" href="javascript:void(0)" style="width: 25%;float: right;margin-left:2%"><img src="https://static.igem.org/mediawiki/2014/c/c9/UCLTANELPIPETTING.JPG" style="max-width: 100%;"></a> -->
<!-- <a data-tip="true" class="top large" data-tip-content="Here's Tanel doing some pipetting in our lab!" href="javascript:void(0)" style="width: 25%;float: right;margin-left:2%"><img src="https://static.igem.org/mediawiki/2014/c/c9/UCLTANELPIPETTING.JPG" style="max-width: 100%;"></a> -->
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<div><strong>Protocols&nbsp;&nbsp;</strong>
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<p>EDO WRITE THINGS HERE</p><br>
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    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">competent cells</span></a>
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    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">transformation</span></a>
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    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">miniprep</span></a>
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    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">digest</span></a>
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    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">gel</span></a></div>
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<p>We began our project by identifying a range of BioBrick parts present in the iGEM 2014 distribution kit which we required as part of our cloning strategy. These parts primarily consisted of both constituitive and inducible promoter systems with ribosome binding sites which we could then use in conjunction with our azo-reductase BioBricks to assemble a functional azo dye degrading gene. We also decided that we would use the Red Florescent Protein expressing BioBrick as a control for any further transformation experiments. As the level of DNA present within each plate of the distribution kit is insufficient to perform digest and ligation reactions on it was necessary to transform each of these plasmids into our NEB5alpha competent cells. After growing our transformed cells overnight we then mini-prepped each of them to obtain BioBrick plasmids at suitable concentrations for future experiments.</p><br>
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     <table border="1px" width="100%" height="auto">
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     <table width="100%" height="auto">
         <thead>
         <thead>
             <tr>
             <tr>
                 <th>  </th>
                 <th>  </th>
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                 <th> Registry ID </th>
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                 <th> Task </th>
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                 <th> Name / Function </th>
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                 <th> Where am I on wiki? </th>
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                <th> Antibiotic Resistance </th>
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                 <th> Where am I on iGEM? </th>
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                <th> Source </th>
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                 <th> Size </th>
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             </tr>
             </tr>
         </thead>
         </thead>
         <tbody>
         <tbody>
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            <!--Lisbon plasmids-->
             <tr>
             <tr>
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                 <td> <center>U</center> </td>
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                 <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e0/UCL_Bronze-metal-star.jpg" width="25px"></center> </td>
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                <td> &nbsp;<a href="http://parts.igem.org/Part:BBa_K314103">BBa_K314103</a> </td>
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                 <td> &nbsp;Team registration </td>
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                 <td> &nbsp;IPTG-inducible LacI Expression Cassette </td>
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                 <td> &nbsp;<a href="/Team:UCL/Humans/Team">Meet our Team!</a> </td>
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                 <td> &nbsp;Chloramphenicol</td>
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                 <td> &nbsp;<a href="https://igem.org/Team.cgi?id=1336">Team Roster!</a> </td>
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                <td> &nbsp;Spring 2014 BioBrick Distribution. Plate 1, Well 4D.</td>
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                 <td> &nbsp;<a href="/Team:UCL/Science/Sequences#BBa_K314103">1638 bp</a> </td>
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             </tr>
             </tr>
             <tr>
             <tr>
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                 <td> <center>T</center> </td>
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                 <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e0/UCL_Bronze-metal-star.jpg" width="25px"></center> </td>
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                <td> &nbsp;<a href="http://parts.igem.org/Part:BBa_J04450">BBa_J04450</a> </td>
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                 <td> &nbsp;Complete Judging form </td>
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                 <td> &nbsp;RFP Coding Device </td>
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                 <td> &nbsp;<a href="/Team:UCL/Project/Achievements">Our Achievements!</a> </td>
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                 <td> &nbsp;Chloramphenicol</td>
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                 <td> &nbsp;<a href="https://igem.org/2014_Judging_Form?id=1336">iGEM Judging form!</a> </td>
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                <td> &nbsp;Spring 2014 BioBrick Distribution. Plate 4, Well 4B. </td>
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                 <td> &nbsp;<a href="/Team:UCL/Science/Sequences#BBa_J04450">1069 bp</a> </td>
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             </tr>
             </tr>
             <tr>
             <tr>
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                 <td> <center>T</center> </td>
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                 <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e0/UCL_Bronze-metal-star.jpg" width="25px"></center> </td>
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                <td> &nbsp;<a href="http://parts.igem.org/Part:BBa_R0010">BBa_R0010</a> </td>
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                 <td> &nbsp;Team Wiki </td>
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                 <td> &nbsp;IPTG-inducible LacI Promoter</td>
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                 <td> &nbsp;<a href="/Team:UCL">Team wiki!</a> </td>
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                 <td> &nbsp;Chloramphenicol</td>
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                 <td> &nbsp;<a href="www.goodbyeazodye.org.uk">See us here also!</a> </td>
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                <td> &nbsp;Spring 2014 BioBrick Distribution. Plate 3, Well 4G.</td>
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                 <td> &nbsp;<a href="/Team:UCL/Science/Sequences#BBa_R0010">200 bp</a> </td>
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             </tr>
             </tr>
             <tr>
             <tr>
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                 <td> <center>T</center> </td>
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                 <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e0/UCL_Bronze-metal-star.jpg" width="25px"></center> </td>
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                <td> &nbsp;<a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a> </td>
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                 <td> &nbsp;We will present our project poster and talk at the iGEM Giant Jamboree, 30 October - 3 November 2014 </td>
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                 <td> &nbsp;Ribosomal Binding Site (RBS)</td>
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                 <td> &nbsp;<a href="/Team:UCL">...</a> </td>
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                 <td> &nbsp;Chloramphenicol</td>
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                 <td> &nbsp;<a href=" ">...</a> </td>
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                <td> &nbsp;Spring 2014 BioBrick Distribution. Plate 4, Well 1N.</td>
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                 <td> &nbsp;<a href="/Team:UCL/Science/Sequences#BBa_B0034">12 bp</a> </td>
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             </tr>
             </tr>
             <tr>
             <tr>
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                 <td> <center>T</center> </td>
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                 <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e0/UCL_Bronze-metal-star.jpg" width="25px"></center> </td>
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                <td> &nbsp;<a href="http://parts.igem.org/Part:BBa_K518012">BBa_K518012</a> </td>
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                 <td> &nbsp;Use of new/improved application of previously existing standard BioBrick Part or Device, and submitted to the iGEM Registry </td>
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                <td> &nbsp;RBS + RFP + double Terminator</td>
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                 <td> &nbsp;<a href="/Team:UCL/Project/Biobricks">...</a> </td>
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                <td> &nbsp;Chloramphenicol</td>
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                 <td> &nbsp;<a href=" ">...</a> </td>
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                <td> &nbsp;Spring 2014 BioBrick Distribution. Plate 1, Well 18C.</td>
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                <td> &nbsp;<a href="/Team:UCL/Science/Sequences#BBa_K518012">828 bp</a> </td>
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            </tr>
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            <tr>
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                <td> <center>N</center> </td>
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                 <td> &nbsp;<a href="http://parts.igem.org/Part:BBa_K206000">BBa_K206000</a> </td>
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                <td> &nbsp;pBAD Strong Promoter</td>
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                <td> &nbsp;Chloramphenicol</td>
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                <td> &nbsp;Spring 2014 BioBrick Distribution. Plate 3, Well 14A.</td>
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                 <td> &nbsp;<a href="/Team:UCL/Science/Sequences#BBa_K206000">130 bp</a> </td>
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            </tr>
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            <tr>
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                <td> <center>! N</center> </td>
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                <td> &nbsp;<a href="http://parts.igem.org/Part:BBa_R0011">BBa_R0011</a> </td>
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                <td> &nbsp;LacI-Regulated, Lambda pL Hybrid Promoter</td>
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                <td> &nbsp;Chloramphenicol</td>
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                <td> &nbsp;Spring 2014 BioBrick Distribution. Plate 2, Well 6D.</td>
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                <td> &nbsp;<a href="/Team:UCL/Science/Sequences#BBa_R0011">55 bp</a> </td>
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            </tr>
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            <tr>
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                <td> <center>! N</center> </td>
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                 <td> &nbsp;<a href="http://parts.igem.org/Part:BBa_B0012">BBa_B0012</a> </td>
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                <td> &nbsp;Transcription Terminator for E. coli RNA Polymerase</td>
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                <td> &nbsp;Chloramphenicol</td>
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                <td> &nbsp;Spring 2014 BioBrick Distribution. Plate 2, Well 2B.</td>
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                <td> &nbsp;<a href="/Team:UCL/Science/Sequences#BBa_B0012">41 bp</a> </td>
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             </tr>
             </tr>
         </tbody>
         </tbody>
     </table>
     </table>
     </font>
     </font>
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    <div><font size="1">Note: U = Used in experiments; T = Used for testing purposes but not for making BioBrick Devices; N = Transformed from Distribution Kits, but not used in experiments; ! = Problematic parts (see Parts Registry), were not used.</font></div>
 
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</div>
</div>
<!--- This is the first biobrick --->
<!--- This is the first biobrick --->
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<div id="view2"><div class="textTitle"><h4>Stage 02: Identification of useful genes for making new BioBricks</h4></div><br>
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<div id="view2"><div class="textTitle"><h4>Silver Achieved!</h4></div><br>
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<strong>Extraction of Bacillus subtilis genomic DNA</strong>
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<p>EDO WRITE THINGS HERE</p><br>
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<div><strong>Protocols&nbsp;&nbsp;</strong>
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    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">DNA extraction</span></a></div>
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<p>Our literature search identified a number of bacterial species that have been proven to degrade azo dye compounds including <i>B. subtilis</i> and <i>P. aeruginosa</i>.  We were able to obtain a <i>B. subtilis</i> strain for use in our project from ?.  We extracted the genomic DNA from this strain using a Promega Wizard Genomic DNA extraction kit so that we could subsequently amplify the azo-reducatase gene (AzoR1) and create our first azo-reductase BioBrick.  After completing the genomic DNA extracton we ran a gel to show that we had successfully extracted the <i>B. subtilis</i> genomic DNA.</p><br>
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    <div>   
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        <div class="accordion">
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            <h4><div class="byline"><i class="icon-user"></i><strong>Extraction of Bacillus subtilis genomic DNA</strong></div></h4>
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                <div>
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                    <div><strong>Protocols&nbsp;&nbsp;</strong>
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                    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">DNA extraction</span></a></div>
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                    <p>Our literature search identified a number of bacterial species that have been proven to degrade azo dye compounds including <i>B. subtilis</i> and <i>P. aeruginosa</i>.  We were able to obtain a <i>B. subtilis</i> strain for use in our project from ?.  We extracted the genomic DNA from this strain using a Promega Wizard Genomic DNA extraction kit so that we could subsequently amplify the azo-reducatase gene (AzoR1) and create our first azo-reductase BioBrick.  After completing the genomic DNA extracton we ran a gel to show that we had successfully extracted the <i>B. subtilis</i> genomic DNA.</p>
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                </div>
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            <h4><div class="byline"><i class="icon-user"></i> Adam Denyer, Tanel Ozdemir &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="June 13, 2014">June 13, 2014</abbr></div></h4>
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                <div>
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                    <p>...</p>
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                </div>
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        </div>
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        <br>
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    </div>
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<!--- This is the second biobrick --->
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<div id="view3"><div class="textTitle"><h4>Stage 03: Transforming E. coli with azo-reductase plasmids</h4></div><br>
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<div><strong>Protocols&nbsp;&nbsp;</strong>
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    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">competent cells</span></a>
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    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">transformation</span></a>
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    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">miniprep</span></a></div>
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    <p>We were gratefully provided with a set of five plasmids from the <a href="http://www.itqb.unl.pt/martins">Microbial & Enzyme Technology Lab</a> led by Dr Lígia O. Martins at the Universidade Nova de Lisboa. They are currently researching how azo-dye degrading enzymes function and are keen to collaborate with us. These plasmids contained a number of genes encoding azo-dye degrading enzymes from both <i>B. subtilis</i> and <i>P. putida</i>  including mutated forms found to exhibit enhanced degradation activity.  As the DNA concentration of the plasmids we were sent was insufficient to perform PCR amplification on we transformed each of these plasmids into our <i>E. coli</i> NEB5alpha derivative competent cells.  After growing the cells overnight we then mini-prepped each of them to obtain plasmids at sufficient concentrations for future experimental work.</p><br>
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     <font size="2">
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     <table border="1px" width="100%" height="auto">
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     <table width="100%" height="auto">
         <thead>
         <thead>
             <tr>
             <tr>
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                 <th> Gene ID</th>
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                 <th> </th>
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                 <th> Name / Function </th>
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                 <th> Task </th>
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                <th> Source </th>
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                 <th> Where am I on wiki? </th>
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                 <th> Size </th>
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                 <th> Where am I on iGEM? </th>
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                 <th> Plasmid </th>
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             </tr>
             </tr>
         </thead>
         </thead>
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             <!--Lisbon plasmids-->
             <!--Lisbon plasmids-->
             <tr>
             <tr>
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                 <td> &nbsp;pAzoR </td>
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                 <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e8/UCL_Silver-metal-star.jpg" width="25px"></center> </td>
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                <td> &nbsp;FMN-dependent NADH-azoreductase 1 </td>
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                 <td> &nbsp;Experimental validation that at least one of our new Biobrick Part(s) or Device(s) works as expected. </td>
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                <td> &nbsp;<em>Pseudomonas putida</em> </td>
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                 <td> &nbsp;<a href="/Team:UCL/Humans/Team">Meet our Team!</a> </td>
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                <td> &nbsp;<a href="http://www.ncbi.nlm.nih.gov/pubmed/21655981">612 bp</a> </td>
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                 <td> &nbsp;<a href="https://igem.org/Team.cgi?id=1336">Team Roster!</a> </td>
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                <td> &nbsp;In expression vector: pET-21a (+) (ampicillin resistant) <a href="http://www.addgene.org/browse/sequence_vdb/2549/ ">[2] <a href=" http://biochem.web.utah.edu/hill/links/pET21.pdf">[3] </a>, <br>initially cloned between <em>NdeI</em> and <em>BamHI</em> restriction sites. </td>
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            </tr>
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            <tr>
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                 <td> &nbsp;p1B6 </td>
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                <td> &nbsp;AzoR heat-stable mutant</td>
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                <td> &nbsp;<em>Pseudomonas putida</em> </td>
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                 <td> &nbsp;<a href="http://www.ncbi.nlm.nih.gov/pubmed/24475252">612 bp</a> </td>
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                 <td> &nbsp;In expression vector: pET-21a (+) (ampicillin resistant) <a href="http://www.addgene.org/browse/sequence_vdb/2549/ ">[2] <a href=" http://biochem.web.utah.edu/hill/links/pET21.pdf">[3] </a>, <br>initially cloned between <em>NdeI</em> and <em>BamHI</em> restriction sites. </td>
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             </tr>
             </tr>
             <tr>
             <tr>
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                 <td> &nbsp;pCotA </td>
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                 <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e8/UCL_Silver-metal-star.jpg" width="25px"></center> </td>
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                <td> &nbsp;Spore Coat Protein Laccase</td>
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                 <td> &nbsp;Characterisation of working Biobrick(s) documented in the "Main Page" section of its Part's/Device's Registry Entry. </td>
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                 <td> &nbsp;<em>Bacillus subtilis</em> </td>
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                 <td> &nbsp;<a href="/Team:UCL/Project/Achievements">Our Achievements!</a> </td>
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                 <td> &nbsp;<a href="http://www.itqb.unl.pt/martins/index_files/JBC2002.pdf">1542 bp</a> </td>
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                 <td> &nbsp;<a href="https://igem.org/2014_Judging_Form?id=1336">iGEM Judging form!</a> </td>
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                 <td> &nbsp;In expression vector: pET-21a (+) (ampicillin resistant (ampR)) <a href="http://www.addgene.org/browse/sequence_vdb/2549/ ">[2] <a href=" http://biochem.web.utah.edu/hill/links/pET21.pdf">[3] </a>, <br>initially cloned between <strong><em>NheI</em></strong> and <em>BamHI</em> restriction sites. </td>
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             </tr>
             </tr>
             <tr>
             <tr>
-
                 <td> &nbsp;pBsDyP </td>
+
                 <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e8/UCL_Silver-metal-star.jpg" width="25px"></center> </td>
-
                <td> &nbsp;Dye Decolourising Peroxidase BSU38260</td>
+
                 <td> &nbsp;New part submitted to iGEM Parts Registry (adhering to the iGEM Registry guidelines). </td>
-
                 <td> &nbsp;<em>Bacillus subtilis</em> </td>
+
                 <td> &nbsp;<a href="/Team:UCL">Team wiki!</a> </td>
-
                 <td> &nbsp;<a href="http://www.ncbi.nlm.nih.gov/pubmed/23820555">1251 bp</a> </td>
+
                 <td> &nbsp;<a href="www.goodbyeazodye.org.uk">See us here also!</a> </td>
-
                 <td> &nbsp;In expression vector: pET-21a (+) (ampicillin resistant) <a href="http://www.addgene.org/browse/sequence_vdb/2549/ ">[2] <a href=" http://biochem.web.utah.edu/hill/links/pET21.pdf">[3] </a>, <br>initially cloned between <em>NdeI</em> and <em>BamHI</em> restriction sites. </td>
+
             </tr>
             </tr>
             <tr>
             <tr>
-
                 <td> &nbsp;pPpDyP </td>
+
                 <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e8/UCL_Silver-metal-star.jpg" width="25px"></center> </td>
-
                <td> &nbsp;Dye Decolourising Peroxidase PP_3248 </td>
+
                 <td> &nbsp;Implications of our project to the environment, security, safety and ethics, and/or ownership and sharing. Also, description of one or more ways these, or broader, implications have been taken into consideration in the design and execution of our project. </td>
-
                 <td> &nbsp;<em>Pseudomonas putida</em> </td>
+
                 <td> &nbsp;<a href="/Team:UCL">...</a> </td>
-
                 <td> &nbsp;<a href="http://www.ncbi.nlm.nih.gov/pubmed/23820555">864 bp</a> </td>
+
                 <td> &nbsp;<a href=" ">...</a> </td>
-
                 <td> &nbsp;In expression vector: pET-21a (+) (ampicillin resistant) <a href="http://www.addgene.org/browse/sequence_vdb/2549/ ">[2] <a href=" http://biochem.web.utah.edu/hill/links/pET21.pdf">[3] </a>, <br>initially cloned between <em>NdeI</em> and <em>BamHI</em> restriction sites. </td>
+
             </tr>
             </tr>
         </tbody>
         </tbody>
Line 228: Line 139:
</div>
</div>
-
<!--- This is the third biobrick --->
+
<!--- This is the second biobrick --->
-
<div id="view4"><div class="textTitle"><h4>Stage 04: Diagnostic digest of azo-reductase plasmids</h4></div><br>
+
<div id="view3"><div class="textTitle"><h4>Gold Achieved!</h4></div><br>
<!-- This is the main text. Anything in a <p>TEXT</p> is a paragraph and will be spaced appropriately-->
<!-- This is the main text. Anything in a <p>TEXT</p> is a paragraph and will be spaced appropriately-->
-
<div><strong>Protocols&nbsp;&nbsp;</strong>
+
<p>EDO WRITE THINGS HERE</p><br>
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">digest</span></a>
+
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">gel</span></a></div>
+
-
    <p>After successfully transforming these plasmids into competent <i>E. coli</i> NEB5alpha cells we then performed a diagnostic digest and gel electrophoresis experiment to ascertain that these plasmids contained the gene we expected.  Each plasmid was digested using two restriction enzymes chosen to digest DNA as specific points on the plasmids and create fragments of known length which we could then confirm using gel electrophoresis.</p>
+
-
</div>
+
-
 
+
-
<!--- This is the fourth biobrick --->
+
-
<div id="view5"><div class="textTitle"><h4>Stage 05: Creation of azo-reductase BioBrick parts from plasmids</h4></div><br>
+
-
<!-- This is the main text. Anything in a <p>TEXT</p> is a paragraph and will be spaced appropriately-->
+
-
<p>...</p><br>
+
-
</div>
+
-
 
+
-
<!--- This is the fifth biobrick --->
+
-
<div id="view6"><div class="textTitle"><h4>Stage 06: Diagnostic digest of azo-reductase BioBrick parts</h4></div><br>
+
-
<!-- This is the main text. Anything in a <p>TEXT</p> is a paragraph and will be spaced appropriately-->
+
-
<p>...</p><br>
+
-
</div>
+
-
 
+
-
<!--- This is the fifth biobrick --->
+
-
<div id="view7"><div class="textTitle"><h4>Stage 07: Assembling azo-reductase BioBrick Device(s)</h4></div><br>
+
-
<!-- This is the main text. Anything in a <p>TEXT</p> is a paragraph and will be spaced appropriately-->
+
-
<p>...</p><br>
+
     <font size="2">
     <font size="2">
-
     <table border="1px" width="100%" height="auto">
+
     <table width="100%" height="auto">
         <thead>
         <thead>
             <tr>
             <tr>
                 <th>  </th>
                 <th>  </th>
-
                 <th> Registry ID </th>
+
                 <th> Task </th>
-
                 <th> Gene ID</th>
+
                 <th> Where am I on wiki? </th>
-
                 <th> Name / Function </th>
+
                 <th> Where am I on iGEM? </th>
-
                <th> Source </th>
+
-
                <th> Size </th>
+
-
                <th> Status </th>
+
             </tr>
             </tr>
         </thead>
         </thead>
Line 269: Line 156:
             <!--Lisbon plasmids-->
             <!--Lisbon plasmids-->
             <tr>
             <tr>
-
                 <td> </td>
+
                 <td> <center><img src="https://static.igem.org/mediawiki/2014/3/36/UCL_Gold-metal-star.jpg" width="25px"></center> </td>
-
                <td> &nbsp;<a href="http://parts.igem.org/Part:BBa_K1336000">BBa_K1336000</a> </td>
+
                 <td> &nbsp;Improve the function OR characterization of an existing BioBrick Part or Device, document this in the "Experience" section of its entry in the iGEM Registry, and submit the part to the iGEM Parts Registry. </td>
-
                <td> &nbsp;AzoR </td>
+
                 <td> &nbsp;<a href="/Team:UCL/Humans/Team">Meet our Team!</a> </td>
-
                <td> &nbsp;FMN-dependent NADH-azoreductase 1 </td>
+
                 <td> &nbsp;<a href="https://igem.org/Team.cgi?id=1336">Team Roster!</a> </td>
-
                 <td> &nbsp;<em>Pseudomonas putida</em> </td>
+
-
                 <td> &nbsp;<a href="/Team:UCL/Science/Sequences#BBa_K1336000">612 bp</a> </td>
+
-
                 <td> &nbsp;[<a href="/Team:UCL/Science/Primers">In Progress</a>]: primers designed </td>
+
             </tr>
             </tr>
             <tr>
             <tr>
-
                 <td> </td>
+
                 <td> <center><img src="https://static.igem.org/mediawiki/2014/3/36/UCL_Gold-metal-star.jpg" width="25px"></center> </td>
-
                <td> &nbsp;<a href="http://parts.igem.org/Part:BBa_K1336001">BBa_K1336001</a> </td>
+
                 <td> &nbsp;Help any registered iGEM team from another school or institute, e.g. characterising a part, debugging a construct, or modelling/simulating their system. </td>
-
                <td> &nbsp;1B6 </td>
+
                 <td> &nbsp;<a href="/Team:UCL/Project/Achievements">Our Achievements!</a> </td>
-
                <td> &nbsp;AzoR heat-stable mutant</td>
+
                 <td> &nbsp;<a href="https://igem.org/2014_Judging_Form?id=1336">iGEM Judging form!</a> </td>
-
                 <td> &nbsp;<em>Pseudomonas putida</em> </td>
+
-
                 <td> &nbsp;<a href="/Team:UCL/Science/Sequences#BBa_K1336001">612 bp</a> </td>
+
-
                 <td> &nbsp;[<a href="/Team:UCL/Science/Experiments">In Progress</a>]: to remove 2 illegal PstI sites </td>
+
             </tr>
             </tr>
             <tr>
             <tr>
-
                 <td> </td>
+
                 <td> <center><img src="https://static.igem.org/mediawiki/2014/3/36/UCL_Gold-metal-star.jpg" width="25px"></center> </td>
-
                <td> &nbsp;<a href="http://parts.igem.org/Part:BBa_K1336002">BBa_K1336002</a> </td>
+
                 <td> &nbsp;Describe a novel approach of our team to help us and others consider the implications/aspects of the design and outcomes of synthetic biology efforts; justifying its novelty and how this approach might be adapted and scaled for others to use. </td>
-
                 <td> &nbsp;CotA </td>
+
                 <td> &nbsp;<a href="/Team:UCL">Team wiki!</a> </td>
-
                <td> &nbsp;Spore Coat Protein Laccase</td>
+
                 <td> &nbsp;<a href="www.goodbyeazodye.org.uk">See us here also!</a> </td>
-
                <td> &nbsp;<em>Bacillus subtilis</em> </td>
+
-
                 <td> &nbsp;<a href="/Team:UCL/Science/Sequences#BBa_K1336002">1542 bp</a> </td>
+
-
                 <td> &nbsp;[<a href="/Team:UCL/Science/Primers">In Progress</a>]: primers designed </td>
+
             </tr>
             </tr>
 +
        </tbody>
 +
    </table>
 +
    </font>
 +
</div>
 +
 +
<!--- This is the third biobrick --->
 +
<div id="view4"><div class="textTitle"><h4>Special Awards</h4></div><br>
 +
<!-- This is the main text. Anything in a <p>TEXT</p> is a paragraph and will be spaced appropriately-->
 +
<p>EDO WRITE THINGS HERE</p><br>
 +
    <font size="2">
 +
    <table width="100%" height="auto">
 +
        <thead>
             <tr>
             <tr>
-
                 <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e0/UCL_Bronze-metal-star.jpg" width="25px"></center> </td>
+
                 <th> </th>
-
                 <td> &nbsp;<a href="http://parts.igem.org/Part:BBa_K1336003">BBa_K1336003</a> </td>
+
                 <th> Task </th>
-
                 <td> &nbsp;BsDyP </td>
+
                 <th> Where am I on wiki? </th>
-
                 <td> &nbsp;Dye Decolourising Peroxidase BSU38260</td>
+
                 <th> Where am I on iGEM? </th>
-
                <td> &nbsp;<em>Bacillus subtilis</em> </td>
+
-
                <td> &nbsp;<a href="/Team:UCL/Science/Sequences#BBa_K1336003">1251 bp</a> </td>
+
-
                <td> &nbsp;[<a href="/Team:UCL/Science/Experiments">New BioBrick Part</a>]: submitted </td>
+
             </tr>
             </tr>
 +
        </thead>
 +
        <tbody>
 +
            <!--Lisbon plasmids-->
             <tr>
             <tr>
-
                 <td> </td>
+
                 <td> <center><img src="https://static.igem.org/mediawiki/2014/3/36/UCL_Gold-metal-star.jpg" width="25px"></center> </td>
-
                <td> &nbsp;<a href="http://parts.igem.org/Part:BBa_K1336004">BBa_K1336004</a> </td>
+
                 <td> &nbsp;Best Supporting Art and Design </td>
-
                <td> &nbsp;PpDyP </td>
+
                 <td> &nbsp;<a href="/Team:UCL/Humans/Team">Meet our Team!</a> </td>
-
                <td> &nbsp;Dye Decolourising Peroxidase PP_3248 </td>
+
                 <td> &nbsp;<a href="https://igem.org/Team.cgi?id=1336">Team Roster!</a> </td>
-
                 <td> &nbsp;<em>Pseudomonas putida</em> </td>
+
-
                 <td> &nbsp;<a href="/Team:UCL/Science/Sequences#BBa_K1336004">864 bp</a> </td>
+
-
                 <td> &nbsp;[<a href="/Team:UCL/Science/Primers">In Progress</a>]: primers designed </td>
+
             </tr>
             </tr>
             <tr>
             <tr>
-
                 <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e0/UCL_Bronze-metal-star.jpg" width="25px"></center> </td>
+
                 <td> <center><img src="https://static.igem.org/mediawiki/2014/3/36/UCL_Gold-metal-star.jpg" width="25px"></center> </td>
-
                 <td> &nbsp;<a href="http://parts.igem.org/Part:BBa_K1336005">BBa_K1336005</a> </td>
+
                 <td> &nbsp;Best Policy and Practice Advance </td>
-
                <td> &nbsp;ispB RNAi </td>
+
                 <td> &nbsp;<a href="/Team:UCL/Project/Achievements">Our Achievements!</a> </td>
-
                <td> &nbsp;RNAi of Octaprenyl Diphosphate <br>Synthase fragment </td>
+
                 <td> &nbsp;<a href="https://igem.org/2014_Judging_Form?id=1336">iGEM Judging form!</a> </td>
-
                <td> &nbsp;<em>Escherichia coli, K12 strain</em> </td>
+
-
                 <td> &nbsp;<a href="/Team:UCL/Science/Sequences#BBa_K1336005">562 bp</a> </td>
+
-
                 <td> &nbsp;[<a href="/Team:UCL/Science/Experiment">New BioBrick Part</a>]: submitted </td>
+
-
            </tr>
+
-
            <tr>
+
-
                <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e0/UCL_Bronze-metal-star.jpg" width="25px"></center> </td>
+
-
                <td> &nbsp;<a href="http://parts.igem.org/Part:BBa_K1336006">BBa_K1336006</a> </td>
+
-
                <td> &nbsp;LacIEC+ispB </td>
+
-
                <td> &nbsp;IPTG inducible ispB RNAi </td>
+
-
                <td> &nbsp;<em>Escherichia coli, K12 strain </em> </td>
+
-
                <td> &nbsp;<a href="/Team:UCL/Science/Sequences#BBa_K1336006">2208 bp</a> </td>
+
-
                <td> &nbsp;[<a href="/Team:UCL/Science/Experiment">New BioBrick Device</a>]: submitted </td>
+
-
            </tr>
+
-
            <tr>
+
-
                <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e0/UCL_Bronze-metal-star.jpg" width="25px"></center> </td>
+
-
                <td> &nbsp;<a href="http://parts.igem.org/Part:BBa_K1336007">BBa_K1336007</a> </td>
+
-
                <td> &nbsp;LacIEC+BsDyP </td>
+
-
                <td> &nbsp;IPTG inducible BsDyP </td>
+
-
                <td> &nbsp;<em>Bacillus subtilis</em> </td>
+
-
                <td> &nbsp;<a href="/Team:UCL/Science/Sequences#BBa_K1336007">2895 bp</a> </td>
+
-
                <td> &nbsp;[<a href="/Team:UCL/Science/Experiment">New BioBrick Device</a>]: submitted </td>
+
-
            </tr>
+
-
            <tr>
+
-
                <td>  </td>
+
-
                <td> &nbsp;<a href="http://parts.igem.org/Part:BBa_K729006">BBa_K729006</a> </td>
+
-
                <td> &nbsp;CueO </td>
+
-
                <td> &nbsp;Laccase </td>
+
-
                <td> &nbsp;<em>Escherichia coli </em> </td>
+
-
                <td> &nbsp;<a href="/Team:UCL/Science/Sequences#BBa_K729006">1612 bp</a> </td>
+
-
                <td> &nbsp;[<a href="/Team:UCL/Science/Experiment">In Progress</a>]: ascertaining identity </td>
+
-
            </tr>
+
-
            <tr>
+
-
                <td> <center>(<img src="https://static.igem.org/mediawiki/2014/3/36/UCL_Gold-metal-star.jpg" width="25px">)</center> </td>
+
-
                <td> &nbsp;<a href="http://parts.igem.org/Part:BBa_K500000">BBa_K500000</a> </td>
+
-
                <td> &nbsp;LiP </td>
+
-
                <td> &nbsp;Lignin Peroxidase </td>
+
-
                <td> &nbsp;<em>Phanerochaete chrysosporium</em> </td> <!-- <br>(White-Rot Fungi) -->
+
-
                <td> &nbsp;<a href="/Team:UCL/Science/Sequences#BBa_K500000">1116 bp</a> </td> <!--Check size!-->
+
-
                <td> &nbsp;[<a href="/Team:UCL/Science/Results">Improved Characterisation</a>]: toxicity issues in gene synthesis. <br>&nbsp;[<a href="/Team:UCL/Science/Experiment">In Progress</a>]: to subclone into pSB1C3/pSB3C5. </td>
+
             </tr>
             </tr>
             <tr>
             <tr>
                 <td> <center><img src="https://static.igem.org/mediawiki/2014/3/36/UCL_Gold-metal-star.jpg" width="25px"></center> </td>
                 <td> <center><img src="https://static.igem.org/mediawiki/2014/3/36/UCL_Gold-metal-star.jpg" width="25px"></center> </td>
-
                 <td> &nbsp;<a href="http://parts.igem.org/Part:BBa_K729004">BBa_K729004</a> </td>
+
                 <td> &nbsp;Best Supporting Software </td>
-
                <td> &nbsp;nucB </td>
+
                 <td> &nbsp;<a href="/Team:UCL">Team wiki!</a> </td>
-
                <td> &nbsp;Extracellular nuclease </td>
+
                 <td> &nbsp;<a href="www.goodbyeazodye.org.uk">See us here also!</a> </td>
-
                <td> &nbsp;<em>Staphylococcus aureus</em> </td>
+
-
                 <td> &nbsp;<a href="/Team:UCL/Science/Sequences#BBa_K729004">561 bp</a> </td>
+
-
                 <td> &nbsp;[<a href="/Team:UCL/Science/Results">Improved Function</a>] </td>
+
             </tr>
             </tr>
         </tbody>
         </tbody>
     </table>
     </table>
     </font>
     </font>
-
</div>
+
   
-
 
+
-
<!--- This is the fifth biobrick --->
+
-
<div id="view8"><div class="textTitle"><h4>Stage 08: Characterisation of azo-reductase BioBrick devices</h4></div><br>
+
-
<!-- This is the main text. Anything in a <p>TEXT</p> is a paragraph and will be spaced appropriately-->
+
-
<p>...</p><br>
+
     <div>
     <div>
-
         <h4><a name="Expt">Placeholder. Will be removed.</a></h4>
+
         <div><img src="https://static.igem.org/mediawiki/2014/e/e0/UCL_Bronze-metal-star.jpg" width="50px">
-
         <strong>Protocols&nbsp;&nbsp;</strong>
+
        <ul>
-
        <a href="/Team:UCL/Science/Proto"><span class="label label-warning">PCR</span></a>
+
         <br>Bronze Medal<br><ul>
-
        <a href="/Team:UCL/Science/Proto"><span class="label label-warning">analytical digest</span></a>
+
            <li> We <a href="https://igem.org/Team.cgi?id=1336">registered</a> our team!</li></br>
-
        <a href="/Team:UCL/Science/Proto"><span class="label label-warning">gel</span></a>
+
            <li> We have completed the <a href="https://igem.org/2014_Judging_Form?id=1336">Judging form!</a></li><br>
-
        (<a href="/Team:UCL/Science/Proto"><span class="label label-warning">digest</span></a>
+
            <li> Our team <a href="/Team:UCL">wiki</a> is here!</li><br>
-
        <a href="/Team:UCL/Science/Proto"><span class="label label-warning">ligation</span></a>
+
            <li> We have prepared a Poster and a Talk for the iGEM Giant Jamboree!</li><br>
-
        <a href="/Team:UCL/Science/Proto"><span class="label label-warning">competent cells</span></a>
+
            <li> Our team <a href="/Team:UCL/Humans/Team">attributions</a> can be found here, with our <a href="/Team:UCL/Humans/Collab">external collaborators</a>, and <a href="/Team:UCL/Humans/Sponsor">sponsors</a></li><br>
-
         <a href="/Team:UCL/Science/Proto"><span class="label label-warning">transformation</span></a>
+
            <li> Our new <a href="/Team:UCL/Project/Biobrick">BioBrick Parts and Devices</a> have been assembled, characterised, and documented!</li>
-
        <a href="/Team:UCL/Science/Proto"><span class="label label-warning">miniprep</span></a>)
+
        </ul>
-
        <br>
+
         </div>
-
        <p>[Insert table of Our Genes]</p>
+
        <div><img src="https://static.igem.org/mediawiki/2014/e/e8/UCL_Silver-metal-star.jpg" width="50px">
-
        <br>
+
            <ul>
-
        <div class="accordion">
+
            <br>Silver Medal<br><ul>
-
            <h4><div class="byline"><i class="icon-user"></i> Adam Denyer, Tanel Ozdemir &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="June 13, 2014">June 13, 2014</abbr></div></h4>
+
                <li> Our new BioBrick Parts and Devices have been <a href="/Team:UCL/Science/Results">experimentally validated</a>, and work as expected!</li></br>
-
                <div>
+
                <li> We documented the characterisation of these parts in the iGEM Parts Registry: <a href="http://parts.igem.org/Part:BBa_K1336003">BBa_1366003</a>, <a href="http://parts.igem.org/Part:BBa_K1336005">BBa_1366005</a>, <a href="http://parts.igem.org/Part:BBa_K1336006">BBa_1366006</a>, <a href="http://parts.igem.org/Part:BBa_K1336007">BBa_1366007</a>.</li><br>
-
                    <p>...</p>
+
                <li> We submitted those new parts to the iGEM Parts Registry.</li><br>
-
                </div>
+
                <li> We discussed how our project could have implications for the environment, security, safety, ethics, ownership and data sharing. Find out more about this in our <a href="/Team:UCL/Humans/Soci">Sociological Imaginations</a> page!</li>
-
             <h4><div class="byline"><i class="icon-user"></i> Adam Denyer, Tanel Ozdemir &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="June 13, 2014">June 13, 2014</abbr></div></h4>
+
            </ul>
-
                 <div>
+
        </div>
-
                    <p>...</p>
+
        <div><img src="https://static.igem.org/mediawiki/2014/3/36/UCL_Gold-metal-star.jpg" width="50px">
-
                </div>
+
            <ul>
 +
             <br>Gold Medal<br><ul>
 +
                <li> We <b>improved</b> the function and characterisation of existing <a href="/Team:UCL/Science/Experiment">BioBrick Parts</a>, and entered this new information into the Registry. However, this has not been submitted to the registry.</li></br>
 +
                <li> We <a href="/Team:UCL/Humans/Collab">collaborated</a> with other registered iGEM teams.</li><br>
 +
                 <li> We outlined and detailed a new approach to tack the issues of <a href="/Team:UCL/Project/Xenobiology">biosafety</a> in the field of Synthetic Biology. We also explained our <https://2014.igem.org/Team:UCL/Humans/Story>story</a>...</li><br>
 +
            </ul>
         </div>
         </div>
-
        <br>
 
     </div>
     </div>
 +
</div>
 +
</div>
</div>
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Latest revision as of 21:03, 17 October 2014

Goodbye Azodye UCL iGEM 2014

jQuery UI Accordion - Default functionality
Achievements

Bronze Achieved!


EDO WRITE THINGS HERE


Task Where am I on wiki? Where am I on iGEM?
 Team registration  Meet our Team!  Team Roster!
 Complete Judging form  Our Achievements!  iGEM Judging form!
 Team Wiki  Team wiki!  See us here also!
 We will present our project poster and talk at the iGEM Giant Jamboree, 30 October - 3 November 2014  ...  ...
 Use of new/improved application of previously existing standard BioBrick Part or Device, and submitted to the iGEM Registry  ...  ...

Silver Achieved!


EDO WRITE THINGS HERE


Task Where am I on wiki? Where am I on iGEM?
 Experimental validation that at least one of our new Biobrick Part(s) or Device(s) works as expected.  Meet our Team!  Team Roster!
 Characterisation of working Biobrick(s) documented in the "Main Page" section of its Part's/Device's Registry Entry.  Our Achievements!  iGEM Judging form!
 New part submitted to iGEM Parts Registry (adhering to the iGEM Registry guidelines).  Team wiki!  See us here also!
 Implications of our project to the environment, security, safety and ethics, and/or ownership and sharing. Also, description of one or more ways these, or broader, implications have been taken into consideration in the design and execution of our project.  ...  ...

Gold Achieved!


EDO WRITE THINGS HERE


Task Where am I on wiki? Where am I on iGEM?
 Improve the function OR characterization of an existing BioBrick Part or Device, document this in the "Experience" section of its entry in the iGEM Registry, and submit the part to the iGEM Parts Registry.  Meet our Team!  Team Roster!
 Help any registered iGEM team from another school or institute, e.g. characterising a part, debugging a construct, or modelling/simulating their system.  Our Achievements!  iGEM Judging form!
 Describe a novel approach of our team to help us and others consider the implications/aspects of the design and outcomes of synthetic biology efforts; justifying its novelty and how this approach might be adapted and scaled for others to use.  Team wiki!  See us here also!

Special Awards


EDO WRITE THINGS HERE


Task Where am I on wiki? Where am I on iGEM?
 Best Supporting Art and Design  Meet our Team!  Team Roster!
 Best Policy and Practice Advance  Our Achievements!  iGEM Judging form!
 Best Supporting Software  Team wiki!  See us here also!

    Silver Medal
    • Our new BioBrick Parts and Devices have been experimentally validated, and work as expected!

    • We documented the characterisation of these parts in the iGEM Parts Registry: BBa_1366003, BBa_1366005, BBa_1366006, BBa_1366007.

    • We submitted those new parts to the iGEM Parts Registry.

    • We discussed how our project could have implications for the environment, security, safety, ethics, ownership and data sharing. Find out more about this in our Sociological Imaginations page!

    Gold Medal
    • We improved the function and characterisation of existing BioBrick Parts, and entered this new information into the Registry. However, this has not been submitted to the registry.

    • We collaborated with other registered iGEM teams.

    • We outlined and detailed a new approach to tack the issues of biosafety in the field of Synthetic Biology. We also explained our story...

Contact Us

University College London
Gower Street - London
WC1E 6BT
Biochemical Engineering Department
Phone: +44 (0)20 7679 2000
Email: ucligem2014@gmail.com

Follow Us

Retrieved from "http://2014.igem.org/Team:UCL/Tests"