Team:Virtus-Parva Mexico

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<a href="https://2014.igem.org/WikitemplateB_parts" style="text-decoration:none;color:#1C140D">PARTS</a></td>  
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<h3>Read about our projects!</h3>
 
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One E.coli Has One Function
 
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What is our project at 2014?
 
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Cadmium is one of harmful materials for us. 50 years ago, itai-iati disease was going around in center of Japan Gihu. The cause was industrial wastewater. Cadmium contained the water. Our project is to find a way to clean contaminated with Cadmium. we think how to clean the water. We use E.coli.
 
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Concretely, we use two kinds of E.coli. One catches Cadmium. the Other makes to all E.coli to use chemoattractant. Catches E.coli displays metallothionein (metallothionein is a protein that combines a heavy metal. Cadomium is one kind of heavy metal). Other is releasing Asp (Asp is one kind of chemoattractant. All E.coli is close to the Asp E.coli).
 
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To use these E.coli. Finally cadomiun is catched(wastewater is be clean).
 
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・How to check E.coli's chemotaxis?
 
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Using soft agar plate.we let E.coli siwm.
 
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・We made a plasmid
 
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we made one plasmid.And we have to make one more plasmid.
 
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<h1><font color=#FFFFFF size=7> Bio-NEMS Drill!</font></h1>
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<h3><p align="left">Traditionally pathogens have been annihilated using pharmaceuticals, but today nanotechnology offers a new possibility with the use of nanoelectromechanical systems (NEMS). We present a novel device that could make these processes possible: <em>the BioNEMS Drill</em>. This is a magnetic portion of chromatin. To construct the device we synthesized hydrophilic magnetite. We used it due to its bio-compatibility for medical applications and later functionalized them with amino groups, which can establish a peptide bond between nanoparticles and a protein. We used the HU histone-like protein, capable of coiling DNA around the nanoparticles. To get enough protein, we modified E. Coli to overexpress it. Since the core of the device is magnetic it can be controlled by applying an external magnetic field. Furthermore, the versatility of the DNA to be coated with various materials and its functionalization can selectively remove certain chemicals inside the human body such as cholesterol or toxic agents.</h3></p>
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Latest revision as of 03:55, 18 October 2014

Virtus Parva

Bio-NEMS Drill!


Traditionally pathogens have been annihilated using pharmaceuticals, but today nanotechnology offers a new possibility with the use of nanoelectromechanical systems (NEMS). We present a novel device that could make these processes possible: the BioNEMS Drill. This is a magnetic portion of chromatin. To construct the device we synthesized hydrophilic magnetite. We used it due to its bio-compatibility for medical applications and later functionalized them with amino groups, which can establish a peptide bond between nanoparticles and a protein. We used the HU histone-like protein, capable of coiling DNA around the nanoparticles. To get enough protein, we modified E. Coli to overexpress it. Since the core of the device is magnetic it can be controlled by applying an external magnetic field. Furthermore, the versatility of the DNA to be coated with various materials and its functionalization can selectively remove certain chemicals inside the human body such as cholesterol or toxic agents.