Team:Groningen/Template/MODULE/projects/parts/detection

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Detection system
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Detection system - LactoAid
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Detection of <i> Pseudonomas aeruginosa</i>(<a href="http://parts.igem.org/Part:BBa_K1365997">BBa_K1365997!</a> , <a href="http://parts.igem.org/Part:BBa_K1365998">BBa_K1365998!</a> &
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We want <i>Lactococcus lactis</i> to start producing growth inhibiting molecules only when a pathogen is present. Therefore, <i>L. lactis</i> must be able to sense <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i>.
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<a href="http://parts.igem.org/Part:BBa_K1365999">BBa_K1365999!</a>)
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Detecting <i>Pseudomonas aeruginosa</i>
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To give our LactoAid the ability to detect<i> Pseudonomas aeruginosa</i> we assembled a new biobrick. For the detection we adopted a widely used principle for detection, namely detection of quorum molecules. P. aeruginosa produces AHLs for communication [Source] and we build an AHL sensor designed for<i> L. lactis</i>.  
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To give <i>Lactococcus lactis</i> the ability to detect <i>P. aeruginosa</i> we assembled a new BioBrick. We adopted a widely used principle for detection, namely detection of quorum molecules. <i>P. aeruginosa</i> produces AHLs for communication<sup>1</sup>, therefore we have built an <a href="http://parts.igem.org/Part:BBa_K1365997">AHL sensor</a>) that can be used by <i>L. lactis</i>.  
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To test the how well our LactoAid detects <i> Pseudonomas aeruginosa</i>we build a construct which has the AHL senor ability coupled with an super fold GFP.
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To test the how well <i>Lactococcus lactis</i> can detect <i>P. aeruginosa</i> we build a construct which has the <a href="http://parts.igem.org/Part:BBa_K1365998">AHL sensor ability coupled with a GFP gene</a>.
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The third part made for the detection is an AHL generator. Many iGEM teams are working with AHL sensing, either to detected <i> Pseudonomas aeruginosa</i>or for an regulatory mechanism. Our AHL generator produces AHLs which enables other teams to produce AHLs and test their sensors without the hassle of working with the pathogenic bacterium itself.   
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A related part that was made is the <a href="http://parts.igem.org/Part:BBa_K1365998">AHL generator</a>. Many iGEM teams are working with AHL sensing, either to detect <i>P. aeruginosa</i> or for an regulatory mechanisms. Our AHL generator produces AHLs, which enables other teams to produce AHLs and test their sensors without the hassle of working with the pathogenic bacterium itself.   
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Latest revision as of 01:50, 18 October 2014

Detection system
 
We want Lactococcus lactis to start producing growth inhibiting molecules only when a pathogen is present. Therefore, L. lactis must be able to sense Pseudomonas aeruginosa and Staphylococcus aureus.
 
Detecting Pseudomonas aeruginosa
To give Lactococcus lactis the ability to detect P. aeruginosa we assembled a new BioBrick. We adopted a widely used principle for detection, namely detection of quorum molecules. P. aeruginosa produces AHLs for communication1, therefore we have built an AHL sensor) that can be used by L. lactis.
 
To test the how well Lactococcus lactis can detect P. aeruginosa we build a construct which has the AHL sensor ability coupled with a GFP gene.
 
A related part that was made is the AHL generator. Many iGEM teams are working with AHL sensing, either to detect P. aeruginosa or for an regulatory mechanisms. Our AHL generator produces AHLs, which enables other teams to produce AHLs and test their sensors without the hassle of working with the pathogenic bacterium itself.
 
 
 
 

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