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Team:Heidelberg/Notebook/Materials
From 2014.igem.org
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Latest revision as of 20:49, 16 October 2014
MATERIALS
– and what else you might need ...
See below all the Materials we used. For the individual experiments visit our Notebook, the MidnightDoc, for a collection of our Methods please see Methods.
Kits
| Kit | Supplier | Catalog Number |
|---|---|---|
| MinElute® PCR Purification Kit (250) | QIAGEN | 28006 |
| Plasmid Plus Maxi Kit (25) | QIAGEN | 12963 |
| Plasmid Plus Midi Kit (25) | QIAGEN | 12943 |
| QIAEX II® Gel Extraction Kit (500) | QIAGEN | 20051 |
| QIAGEN® Plasmid Plus Midi Kit (100) | QIAGEN | 12945 |
| QIAquick® Gel Extraction Kit (250) | QIAGEN | 28706 |
| QIAquick® PCR Purification Kit (250) | QIAGEN | 28106 |
| QIAprep® Spin Miniprep Kit (250) | QIAGEN | 27106 |
| QIAprep® Spin Miniprep Columns | QIAGEN | 27115 |
| Protein Thermal Shift Starter Kit | 4462263 | Life Technologies |
| EnzChek® Ultra Xylanase Assay Kit | Life Technologies | E33650 |
Marker
| Marker | Supplier | Catalog Number |
|---|---|---|
| Quick-Load® 2-Log DNA Ladder (0.1-10.0 kb) | New England BioLabs | N3200S |
| Quick-Load® 1 kb DNA Ladder | New England BioLabs | N0468S |
| 50 bp DNA Ladder | New England BioLabs | N3236S |
| Gel loading solution | Sigma-Aldrich Chemie GmbH | G2526-5ML |
| MagicMark XP Western Protein Standard | Life Technologies | LC5602 |
| Novex® Sharp Pre-stained Protein Standard | Invitrogen | LC5800 |
| SERVA Triple Color Protein Standard | SERVA Electrophoresis | 39258.01 |
Enzymes
| Enzyme | Supplier | Catalog Number |
|---|---|---|
| DreamTaq Green PCR Master Mix (2X) | Thermo Fisher Scientific Biosciences GmbH | K1081 |
| DreamTaq PCR MM | Fermentas Life Sciences | K1071 |
| Gibson Assembly® Master Mix | New England Biolabs | E2611 S |
| Lysozyme from Chicken Egg White | Sigma-Aldrich Chemie GmbH | L4919-500MG |
| Phusion® Flash High-Fidelity PCR Master Mix | Biozym Scientific GmbH | F-548L |
| Phusion® High-Fidelity PCR Master Mix | New England Biolabs | M0531 L |
| T4 DNA Ligase | New England Biolabs GmbH | M0202 S |
| 2x PCR Master mix Solution (iTaq) | HISS DIAGNOSTICS GmbH | 25028 |
| Sortase A (Staphylococcus aureus) | roboklon | E4400-01 |
| TEV Protease | Th. Geyer GmbH | SA/T4455/000001 |
| Xylanase, recombinant | Sigma-Aldrich Chemie GmbH | X2753-10G |
| Human DNA (cytosine-5) Methyltransferase | NEB | M0230 S |
Antibodies
| Antibody | Supplier | Catalog Number |
|---|---|---|
| Penta·His Antibody, BSA-free | QIAGEN | 34660 |
| Anti-GFP | Roche | 11814460001 |
| RFP antibody [5F8] | Chromotec | 5F8 |
| Goat anti Mouse IgG | HRPO Dianova GmbH | 115-035-003 |
| Rat IgG, HRP-linked whole antibody (from goat) | GE Healthcare | NA935 |
Restriction Enzymes
| Enzyme | Supplier | Catalog Number |
|---|---|---|
| BamI | New England Biolabs | R3136 S |
| BgIII | New England Biolabs | R0144 S |
| BsaI-HF® | NEB | R3535 L |
| BsmBI | NEB | R0580 L |
| DpnI | New England Biolabs | R0176 S |
| EcoRI | New England BioLabs | R0101S |
| EcoRI-HF | New England Biolabs | R3101 |
| HindIII-HF | New England Biolabs | R3104 S |
| HpaII | NEB | R0171 S |
| KpnI-HF | New England Biolabs | R3142 S |
| MfeI-HF | New England Biolabs | R3589 S |
| NheI-HF | New England BioLabs | R3131 S |
| NotI-HF | New England BioLabs | R3189 S |
| PacI | New England Biolabs | R0547 S |
| PstI-HF | New England Biolabs | R3140 S |
| PvuI-HF | New England BioLabs | R3150S |
| SalI-HF | New England Biolabs | R3138 S |
| Sau3AI | NEB | R0169 S |
| SpeI-HF | New England BioLabs | R3133 S |
| XbaI | New England BioLabs | R0145 S |
Bacterial Strains
| Strain | Supplier | Catalog Number |
|---|---|---|
| E. coli DH10ß | New England Biolabs | C3019 |
| E. coli Top10 | Invitrogen | C404010 |
| E. coli Turbos | ||
| E. coli MG1655 | ||
| E. coli OneShot | ||
| E. coli Bl21 (DE3) | ||
| Bacillus subtilis | ||
| Micrococcus lysodeikticus |
Antibiotics and Media Supplements
| Antibiotic | Supplier | Catalog Number | Concentration stock solution | Dilution | Solvent |
|---|---|---|---|---|---|
| Ampicillin Anhydrous Crystalline | Sigma-Aldrich Chemie GmbH | A9393-5G | 100 mg/ml | 1:1000 | H2O |
| Ampicillin Sodium Crystalline | Sigma-Aldrich Chemie GmbH | A9518-5G | 100 mg/ml | 1:1000 | H2O |
| Chloramphenicol Crystalline | Sigma-Aldrich Chemie GmbH | C0378-5G | 30 mg/ml | 1:3000 | Ethanol |
| Kanamycinsulfat Mixture of Componenta | Sigma-Aldrich Chemie GmbH | 60615-5G | 50 mg/ml | 1:1000 | H2O |
| Tetracycline | Sigma-Aldrich Chemie GmbH | T7660 | 10 mg/ml | 1:1000 | Ethanol |
| Propionic Acid Sodium Insect Cell*Culture | Sigma-Aldrich Chemie GmbH | P5436-100G | 100mM | 10mM | H2O |
| Bacitracin | Sigma-Aldrich Chemie GmbH | B0125-50KU | - | - |
Media
| Medium | Supplier | Catalog Number |
|---|---|---|
| SOC Outgrowth Medium | New England Biolabs GmbH | B9020 S |
| LB BROTH BASE | Th. Geyer GmbH & Co KG | SA/L3022/001000 |
| LB Broth Powder | Sigma-Aldrich Chemie GmbH | L3022-1KG |
| M9 Minimal Salts | SERVA | 48505.01 |
Buffers
| Buffer | Supplier | Catalog Number |
|---|---|---|
| NEBuffer Pack #4 (green) | New England Biolabs GmbH | B7004 S |
| NEBuffer Pack #1 (yellow) | New England Biolabs GmbH | B7001 S |
| NEBuffer Pack for T4 DNA Ligase | New England Biolabs GmbH | B0202 S |
| NEBuffer Pack #2 (blue) | New England Biolabs GmbH | B7002 S |
| NEBuffer Pack #3 (red) | New England Biolabs GmbH | B7003 S |
| TAE - Buffer (50X) for Molecular Biology | VWR International GmbH | A4686.1000 |
| Gel Loading Buffer | Sigma-Aldrich | G2526-5ML |
| Tris Acetate-EDTA Buffer | Sigma-Aldrich | T9650-1L |
Other Chemicals
| Chemical | Supplier | Catalog Number |
|---|---|---|
| Isopropyl B-D-Thiogalactopyranoside 1 piece | Sigma-Aldrich Chemie GmbH | I5502-1G |
| Dimethyl Sulfoxide PCR Reagent | Sigma-Aldrich Chemie GmbH | D9170-1VL |
| Glycerol Sigma Grade | Sigma-Aldrich Chemie GmbH | G9012-100ML |
| 5-Bromo-4-Chloro-3-Indolyl B-D-*Galactop | Sigma-Aldrich Chemie GmbH | B4252-100MG |
| Bacteriological Agar | Sigma-Aldrich Chemie GmbH | A5306-250G |
| L-Plus-Arabinose Crystalline | Sigma-Aldrich Chemie GmbH | A3256-25G |
| Calciumchlorid Dihydrat | Th. Geyer GmbH & Co KG | SA/00223506/000500 |
| Malt Extract from Starch Digestion | Sigma-Aldrich Chemie GmbH | M0383-100G |
| D(+)-Saccharose, ACS, for Micro-Biology | Sigma-Aldrich Chemie GmbH | 84100-1KG |
| Dimethyl Sulfoxide Plant Cell Culture*TE | D4540-100ML | |
| Sodium Hydroxide Anhydrous Pellets | Th. Geyer GmbH & Co.KG | SA/S5881/000500 |
| TRIZMA(R) Hydrochloride PH 3.5-5.0 | Sigma-Aldrich Chemie GmbH | T6666-50G |
| L-Glutamine 200 MM Sterile | Sigma-Aldrich Chemie GmbH | G7513-20ML |
| Ethanol 96% Denatured | Carl Roth GmbH & Co.KG | T171.3 |
| Propanol-2 | Sigma-Aldrich Chemie GmbH | 59309-1L |
| Natriumdodecylsulfat,SDS,99%, Ultra Pure | 13904 | |
| Gold(III)-Chloride | Carl Roth GmbH & Co.KG | 5624.1 |
| Pro-Leu | Sigma-Aldrich Chemie GmbH | P1130-1G |
| Nitric Acid 65% p.a. Iso | Carl Roth GmbH & Co.KG | X943.1 |
| Mops, Sodium | Sigma-Aldrich Chemie GmbH | M9024-25G |
| L-Glutamine | Sigma-Aldrich Chemie GmbH | G7513-100ML |
| Chelating Resin | Sigma-Aldrich Chemie GmbH | C7901-50G |
| Potassium Hydroxide in Platellets | 6751.3 | |
| Hydrochloric Acid 37% | 4625.1 | |
| Pyruvic Acid | Sigma-Aldrich Chemie GmbH | 107360-25G |
| Fmoc-Orn(BOC)-OH 96.0 % | 47560-5G-F | |
| Glycerol >99.5% | Sigma-Aldrich Chemie GmbH | G9012-1L |
| Water Molecular Biology Reagent | Sigma-Aldrich Chemie GmbH | W4502-1L |
| Acetonitrile | Sigma-Aldrich Chemie GmbH | 34967-1L |
| Ascorbic Acid 99% | Sigma-Aldrich Chemie GmbH | A92902-100G |
| Imidazol 99% | Carl Roth GmbH | 3899.4 |
| 1,4-Dithiothreit p.a. 25g | Carl Roth GmbH | 6908.2 |
| Phenylmethylsulfonylfluorid | Carl Roth GmbH | 6367.1 |
| S-Adenosyl-L-Methionine Chloride (SAM) | Sigma-Aldrich Chemie GmbH | A7007-5MG |
| Boric Acid | Carl Roth GmbH & Co.KG | 6943.1 |
| Fluorecine Isothiocyanate Isomer | Sigma-Aldrich Chemie Gmb | F7250-50MG |
| Hydroxylammonium chloride | Sigma-Aldrich Chemie GmbH | 159417-100G |
| Rotiphorese® Gel 40 (19:1) 250 ml | Carl Roth GmbH | 3030.2 |
| Rotiphorese® Gel 30 (37,5:1) 1 l | Carl Roth GmbH | 3029.1 |
| Albumin Bovine Fraction V Powder | Sigma-Aldrich Chemie GmbH | A9647-50G |
| Peptidoglycan from Micrococcusluteus | Sigma-Aldrich Chemie GmbH | 53243-10MG-F |
| Adenosine 5´-Triphosphate (ATP) | NEB | P0756 S |
Electrophoresis
| Reagent | Supplier | Catalog Number | Concentration | Solvent |
|---|---|---|---|---|
| Agarose Molecular Biology Reagent | Th. Geyer GmbH & Co KG | SA/A9539/000050 | 0.5% | H2O |
| Agarose for Routine Use | Sigma-Aldrich Chemie GmbH | A9539-100G | - | - |
| Gel Loading Dye Purple | NEB | B7024 S |
Primers and Oligos
| Name | Sequence |
|---|---|
| VF2 | tgccacctgacgtctaagaa |
| VR | attaccgcctttgagtgagc |
| pSB1C3_F_SspDnaE-N-taataat | GACGCAGGTACAATTAAATAATAATACTAGTAGCGGCCGCTGCAG |
| pSB1C3_R_Flag-SspDnaE_C | CTTTAACCTTGTCATCATCGTCTTTGTAGTCCATCTAGTACTTTCCTGTGTGACTCTAGAAGCG |
| SspDnaE-C_F_pSB1C3-Flag | TACTAGATGGACTACAAAGACGATGATGACAAGGTTAAAGTTATCGGTCGTCGATCCC |
| SspDnaE-C_R_BsaI-ccdB | CTCCCTTATACACAGCCAGTGGTCTCGAATTGTTTGTTAAAACAGTTGGCGGCGATAG |
| ccdB_F_SspDnaE-C-BsaI | TTAACAAACAATTCGAGACCACTGGCTGTGTATAAGGGAGCCTGAC |
| ccdB_R_BsaI-SspDnaE-N | TTAAAATTTCGGTACCAAAACTGAGGCAGCTAGTGAGACCCGCGTGGATCCGGCTTAC |
| SspDnaE-N_F_ccdB-BsaI | GGTCTCACTAGCTGCCTCAGTTTTGGTACCG |
| SspDnaE-N_R_taataat-pSB1C3 | GCGGCCGCTACTAGTATTATTATTTAATTGTACCTGCGTCAAGTAATG |
| SspDnaE-C_R_6His-BsaI-ccdB | GCCAGTGGTCTCGAATTGATGGTGATGGTGATGGTGTTTGTTAAAACAGTTGGCGGCGATAG |
| ccdB_F_SspDnaE-C-6His-BsaI | CAAACACCATCACCATCACCATCAATTCGAGACCACTGGCTGTGTATAAGGGAGCCTGAC |
| pSB1C3_F_NpuDnaE-N | CGGGTTGATAATTTGCCGAATTAATAATACTAGTAGCGGCCGCTG |
| pSB1C3_R_FLAG-NpuDnaE-C | CTTGTCGTCATCGTCTTTGTAGTCCATCTAGTACTTTCCTGTGTGACTCTAGAAGC |
| NpuDnaE-C_F_pSB1C3-FLAG | AGGAAAGTACTAGATGGACTACAAAGACGATGACGACAAGCATATGATCAAAATAGCCACACG |
| NpuDnaE-C_R_BsaI-ccdB | GCTCCCTTATACACAGCCAGTGGTCTCGAATTGATTGAAACAATTAGAAGCTATGAAGCC |
| ccdB_F_NpuDnaE-C-BsaI | TTTCAATCAATTCGAGACCACTGGCTGTGTATAAGGGAGCC |
| ccdB_R_BasI-NpuDnaE-N | CTTAAACAGCTAGTGAGACCCGCGTGGATCCGGCTTAC |
| NpuDnaE-N_F_BsaI-ccdB | TAGTAAGCCGGATCCACGCGGGTCTCACTAGCTGTTTAAGCTATGAAACGGAAATATTG |
| NpuDnaE-N_R_pSB1C3 | CCGCTACTAGTATTATTAATTCGGCAAATTATCAACCC |
| NpuDnaE-C_R_6His-BsaI-ccdB | TGGTCTCGAATTGATGATGGTGATGGTGATGATTGAAACAATTAGAAGCTATGAAGCC |
| ccdB_F_NpuDnaE-C-6His-BsaI | TGTTTCAATCATCACCATCACCATCATCAATTCGAGACCACTGGCTGTGTATAAGGGAGCC |
| BBa_E0040_F_EcoR1 | GAAAGGAATTCATGCGTAAAGGAGAAGAACTTTTCACTGGAG |
| BBa_E0040_R_Spe1 | TAGTAACTAGTTTTGTATAGTTCATCCATGCCATGTGTAATCC |
| DNMT1_P1_R_deg.seq.731-1602 | GCATCCAGRTTGCTSCC |
| DNMT1_P2_R_deg.seq.731-1602 | CCACCACACAGCATYTC |
| DNMT1_P3_F_both_seq.731-1602 | ATGTGGGACCCGGCAGC |
| DNMT1_P4_F_deg.seq.731-1602 | CAGTAYACCTTTCATGATGTG |
| LOV-I539E_mut_F | TTTTGGTCTCAGAGGCCGCCGCCAAGCGGGTGAAGCTGGAC |
| LOV-I539E_mut_R | ttttggtctcacctcattttctgcagttttcttaatcagcatg |
| LOV-C450A_mut_F | ttttggtctcagccaggtttctacaaggtcctgaaactgatc |
| LOV-C450A_mut_R | aaaaggtctcttggcgtttcttcccaaaatttcttcac |
| T7RBS BioBrick_F | AAACTACGCTTTAGTAGCTTAATAACTCTGATAGTGCTAGTGTAGATCTC |
| T7RBS BioBrick_R | PLACEHOLDER |
| T7RBS-IGEMHD_F | PLACEHOLDER |
| T7RBS-IGEMHD_R | PLACEHOLDER |
| pSBX-T7fwd | AATTTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCGAATTCGCGGCCGCTT |
| pSBX-T7rev | CTAGAAGCGGCCGCGAATTCGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTA |
| T7RBS-IGEMHD_F_v2 | PLACEHOLDER |
| T7RBS-IGEMHD_R_v2 | PLACEHOLDER |
| T7RBS BioBrick_F_v2 | AATTCGCGGCCGCTTCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATA |
| T7RBS BioBrick_R_v2 | CTAGTATCTCCTTCTTAAAGTTAAACAAAATTATTCTCTAGAAGCGGCCGCG |
| Bba_E0040_F_BsaI=EcoR1 | GAAAGGGTCTCGAATTCATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTC |
| Bba_E0040_R_His6_BsaI=Spe1 | TAGTAGGTCTCACTAGTGTGGTGATGGTGATGATGTTTGTATAGTTCATCCATGCCATGTG |
| BsaI=EcoRI_null_BsaI=Spe1 | GCAGCAGGTCTCAAATTATGCGACTAGAGAGACCGCTGCT |
| BsaI=SpeI_null_BsaI=EcoRI | AGCAGCGGTCTCTCTAGTCGCATAATTTGAGACCTGCTGC |
| 1F-smt3_pSB1A3 | AATTCGCGGCCGCTTCTAGATGTCGGACTCAGAAGTCAATCAAGAAGC |
| 2R-smt3_Tev_BsaI_ccdb | TCTCTACCCTGGAAGTACAGGTTTTCGGATCCACCAATCTGTTCTCTGTGAGC |
| 2F-ccdb_F_smt3_Tev_BsaI | AGATTGGTGGATCCGAAAACCTGTACTTCCAGGGTAGAGACCACTGGCTGTGTATAAGGGAGCC |
| 3R-ccdb_R-sortase-his6 | GCCGCTACTAGTATTATTAGTGATGGTGATGGTGATGACCACCGGTTTCCGGAAGTGAGACCCGCGTGGATCCGGCTTACTAAAAGCC |
| 3F-pSB1A3_F_ccdb-sortase-his6 | GGTCATCACCATCACCATCACTAATAATACTAGTAGCGGCCGCTGCAGTCCG |
| 1R-pSB1A3_smt3 | TTCTTGATTGACTTCTGAGTCCGACATCTAGAAGCGGCCGCGAATTCC |
| ccdB_F_BsmBI | AGAGACGACTGGCTGTGTATAAGGGAGCCTGAC |
| ccdB_R_BsmBI | TGAGACGCGCGTGGATCCGGCTTACTAAAAG |
| mRFP_F_BsaI | AGAGACCCAATACGCAAACCGCCTCTC |
| mRFP_R_BsaI | TGAGACCTATAAACGCAGAAAGGCCCACC |
| T7RBS-IGEMHDv3_F | AATTCGCGGCCGCTTCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATACTAGATGATCGGTGAAATGCACGACTGATAGTA |
| T7RBS-IGEMHDv3_R | CTAGTACTATCAGTCGTGCATTTCACCGATCATCTAGTATCTCCTTCTTAAAGTTAAACAAAATTATTCTCTAGAAGCGGCCGCG |
| NpuDnaEN_F | GTGGGCCTTTCTGCGTTTATAGGTCTCATGCTTAAGCTATGAAACGGAAATATTGACAG |
| NpuDnaEN_R | GCTCCCTTATACACAGCCAGTCGTCTCTACCACCATTCGGCAAATTATCAACCCGCATC |
| NpuDnaEC_F | GCCGGATCCACGCGCGTCTCAGGTGGAATCAAAATAGCCACACGTAAATATTTAGGCAAAC |
| NpuDnaEC_R | GAGAGGCGGTTTGCGTATTGGGTCTCTGTTGGAAGCTATGAAGCCATTTTTGAGTGC |
| SspDnaEN_F | PLACEHOLDER |
| SspDnaEN_R | PLACEHOLDER |
| SspDnaEC_F | PLACEHOLDER |
| SspDnaEC_R | PLACEHOLDER |
| AssemBIyIntN-linker_F | TGGTTAGTAGGGTGGC |
| AssemBIyIntN-linker_R | ATTAGCCACCCTACTA |
| AssemBIyIntC-linker_F | GATGGGTGGCGGTGGC |
| AssemBIyIntC-linker_R | CACCGCCACCGCCACC |
| SB-prep-3P-1 | gccgctgcagtccggcaaaaaa |
| SB-prep-2Ea | atgaattccagaaatcatccttagcg |
| pSBX_F_ccdB-BsmBI | GCCGGATCCACGCGCGTCTCATAATAAATCGGTGAAATGCACGACTGATAG |
| pSBX_R_BsmBI-ccdB | GCTCCCTTATACACAGCCAGTCGTCTCTCATCTAGTATCTCCTTCTTAAAGTTAAACAAAATTATTCTC |
| pSBX_F_mRFP-BsaI | GTGGGCCTTTCTGCGTTTATAGGTCTCATAATAAATCGGTGAAATGCACGACTGATAG |
| pSBX_R_BsaI-mRFP | GAGAGGCGGTTTGCGTATTGGGTCTCTCATCTAGTATCTCCTTCTTAAAGTTAAACAAAATTATTCTC |
| pSBX_F_taataa | TAATAAATCGGTGAAATGCACGACTGATAG |
| pSBX_R_atg | CATCTAGTATCTCCTTCTTAAAGTTAAACAAAATTATTCTC |
| GFP_F_pSBX | TCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATACTAGATGATGCGTAAAGGAGAAGAACTTTTC |
| GFP_R_pSBX | CTAGTACTATCAGTCGTGCATTTCACCGATTTATTATTTGTATAGTTCATCCATGCCATG |
| Lambda-Lys_F_pSBX | TCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATACTAGATGGTAGAAATCAATAATCAACGTAAGGC |
| Lambda-Lys_R_pSBX | CTAGTACTATCAGTCGTGCATTTCACCGATTTATTATACATCAATCTCTCTGACCGTTCCG |
| 1F-ccdb_F_pSB1A3-bsaI | ATTTCTGGAATTCGCGGCCGCTTCTAGATGTGAGACCACTGGCTGTGTATAAGGGAGCC |
| 1R-pSB1A3_R_bsaI-sortase-his-pSB1A3 | TTATACACAGCCAGTGGTCTCACATCTAGAAGCGGCCGCGAATTCC |
| SspDnaX-S11C_F | TTAGTAAGCCGGATCCACGCGCGTCTCAGGTGGTGGCCTGCTGGTACACAACAGAGACCCAATACGCAAACCGCCTCTCCCC |
| SspDnaX-S11C_R | GGGGAGAGGCGGTTTGCGTATTGGGTCTCTGTTGTGTACCAGCAGGCCACCACCTGAGACGCGCGTGGATCCGGCTTACTAA |
| SspGyrB-S11C_F | TTAGTAAGCCGGATCCACGCGCGTCTCAGGTGGTGGTGTTTTCGTTCACAACAGAGACCCAATACGCAAACCGCCTCTCCCC |
| SspGyrB-S11C_R | GGGGAGAGGCGGTTTGCGTATTGGGTCTCTGTTGTGAACGAAAACACCACCACCTGAGACGCGCGTGGATCCGGCTTACTAA |
| TerDnaE3-S11C_F | TTAGTAAGCCGGATCCACGCGCGTCTCAGGTGGTGGTCTGATCGCGAGCAACAGAGACCCAATACGCAAACCGCCTCTCCCC |
| TerDnaE3-S11C_R | GGGGAGAGGCGGTTTGCGTATTGGGTCTCTGTTGCTCGCGATCAGACCACCACCTGAGACGCGCGTGGATCCGGCTTACTAA |
| NpuDnaEN_R_taataa-pSBX | CTAGTACTATCAGTCGTGCATTTCACCGATTTATTAATTCGGCAAATTATCAACCCGCATC |
| NpuDnaEC_F_pSB-T7RBS | TCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATACTAGATGATCAAAATAGCCACACGTAAATATTTAGGC |
| AssemBIyIntN-linker+H6_F | TGGTGGTCATCATCACCATCACCATTAG |
| AssemBIyIntN-linker+H6_R | ATTACTAATGGTGATGGTGATGATGACC |
| AssemBIyIntC-linker+H6_F | GATGCATCATCACCATCACCACGGT |
| AssemBIyIntC-linker+H6_R | CACCACCGTGGTGATGGTGATGATG |
| GFPn_1-157_R_KGG_TGCT_BsaI | CGTAGGTCTCGAGCACTTACCTCCTTTGTCTGCCATGATGTATACATTG |
| GFPn_1-64_R_KGG_TGCT_BsaI | CGTAGGTCTCAAGCACTTACCTCCGAAAGTAGTGACAAGTGTTGGCC |
| GFPn_1-64_1-157_F_H6_GATG_BsaI | GCATGGTCTCAGATGCATCATCACCATCACCACCGTAAAGGAGAAGAACTTTTC |
| GFPc_158-238_F_NWC_CAAC_BsaI | GCATGGTCTCACAACTGCTGGAACCAAAAGAATGGAATCAAAGTTAAC |
| GFPc_65-238_F_NWC_CAAC_BsaI | GCATGGTCTCACAACTGCTGGAACGGTTATGGTGTTCAATGCTTTGC |
| GFPc_65-238_158-238_R_H6_TAAT_BsaI | CGTAGGTCTCTATTAGTGGTGATGGTGATGATGTTTGTATAGTTCATCCATGCCATGTG |
| mCherryN_1-168_R_KGG_TGCT_BsaI | CGTAGGTCTCAAGCACTTACCTCCCTGCTTGATCTCGCCCTTC |
| mCherryN_1-168_F_H6_GATG_BsaI | GCATGGTCTCAGATGCATCATCACCATCACCACGTGAGCAAGGGCG |
| mCherryC_169-236_R_H6_TAAT_BsaI | CGTAGGTCTCTATTAGTGGTGATGGTGATGATGCTTGTACAGCTCGTCCATGC |
| mCherryC_169-236_F_NWC_CAAC_BsaI | GCATGGTCTCACAACTGCTGGAACAGGCTGAAGCTGAAGGACG |
| mRFPn_1-168_R_KGG_TGCT_BsaI | CGTAGGTCTCAAGCACTTACCTCCTTTCAGTTTCAGACGCATTTTGATTTC |
| mRFPn_1-154_R_KGG_TGCT_BsaI | CGTAGGTCTCAAGCACTTACCTCCGTCTTCCGGGTACATACGTTCG |
| mRFPn_1-154_1-168_F_H6_GATG_BsaI | GCATGGTCTCAGATGCATCATCACCATCACCACGCTTCCTCCGAAGACG |
| mRFPc_169-225_F_NWC_CAAC_BsaI | GCATGGTCTCACAACTGCTGGAACGACGGTGGTCACTACGACG |
| mRFPc_155-225_F_NWC_CAAC_BsaI | GCATGGTCTCACAACTGCTGGAACGGTGCTCTGAAAGGTGAAATC |
| mRFPc_155-225_169-225_R_H6_TAAT_BsaI | CGTAGGTCTCTATTAGTGGTGATGGTGATGATGAGCACCGGTGGAGTG |
| RenLucN_1-229_R_KGG_TGCT_BsaI | CGTAGGTCTCAAGCACTTACCTCCCTTAACGAGAGG |
| RenLucN_1-229_F_H6_GATG_BsaI | GCATGGTCTCAGATGCATCATCACCATCACCACGCTTCCAAGGTGTACG |
| RenLucC_230-311_R_H6_TAAT_BsaI | CGTAGGTCTCTATTAGTGGTGATGGTGATGATGTTACTGCTCGTTCTTCAGCACG |
| RenLucC_230-311_F_NWC_CAAC_BsaI | GCATGGTCTCACAACTGCTGGAACAAGCCCGACGTCGTCC |
| FireLucN_1-437_R_KGG_TGCT_BsaI | CGTAGGTCTCAAGCACTTACCTCCGCGGTCAACGATGAAG |
| FireLucN_1-437_F_H6_GATG_BsaI | GCATGGTCTCAGATGCATCATCACCATCACCACGAAGACGCCAAAAAC |
| FireLucC_438-550_R_H6_TAAT_BsaI | CGTAGGTCTCTATTAGTGGTGATGGTGATGATGCACGGCGATCTTTCC |
| FireLucC_438-550_F_F_NWC_CAAC_BsaI | GCATGGTCTCACAACTGCTGGAACCTGAAGTCTCTGATTAAGTACAAAGGC |
| PCA(t7rbs-npuc-mrfp-npun-igemhd)_F_prefix | CCCCGAATTCGCGGCCGCTTCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATACTAGATG |
| PCA(t7rbs-npuc-mrfp-npun-igemhd)_R_SpeI | CGCTACTAGTACTATCAGTCGTGCATTTCACCG |
| LambdaLys_F_BsaI_c-ext | CTGGTCTCACAACTGCTGGGAAGTAGAAATCAATAATCAACGTAAGGCGTTCC |
| LambdaLys_R_HISTAG_n-ext_BsaI | CTGGTCTCTAGCATTTACCACGGCCGCTGTGATGATGATGATGATGGCTGCCTACATCAATCTCTCTGACCGTTCCG |
| LambdaLys_R_RIGIDLINKER_n-ext_BsaI | CTGGTCTCTAGCATTTACCACGCGGTGCTGCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGCGCCACCTACATCAATCTCTCTGACCGTTCCG |
| LambdyLys_R_FLEXLINKER_n-ext_BsaI | CTGGTCTCTAGCATTTACCACGTCCAGAACCACCACCAGAACCACCTACATCAATCTCTCTGACCGTTCCG |
| Xyla_BB_F_RBS34_X | GCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCTAGCACAGACTACTGGCAAAATTG |
| Xyla_BB_R_SP | GGAAGCCTGCAGCGGCCGCTACTAGTATTATTACCACACTGTTACGTTAGAACTTCCAC |
| Xyla_F_BsaI_C-ext | CTGGTCTCACAACTGCTGGGAAGCTAGCACAGACTACTGGCAAAATTG |
| Xyla_F_BsaI_Destroyed-C-ext_His | CTGGTCTCACAACGGCTGGGAAGCTAGCACAGACTACTGGCAAAATTG |
| Xyla_R_BsaI_N-ext | CTGGTCTCTAGCATTTACCACGCCACACTGTTACGTTAGAACTTCCAC |
| Xyla_R_BsaI_N-ext_His | CTGGTCTCTAGCATTTACCACGATGATGGTGATGGTGATGCCACACTGTTACGTTAGAACTTCCAC |
| 2GateNinsert_F_X-T7RBS | CCGCTTCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATACTAGATGAGAGACCCAATACGCAAACC |
| 2GateNinsert_R_SP | CCGGACTGCAGCGGCCGCTACTAGTATTTATTATGAGACGCGCGTGGATC |
| 2GateCinsert_F_X-T7RBS | CGCTTCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATACTAGATGAGAGACGACTGGCTGTGTATAAGG |
| 2GateCinsert_R_SP | CCGGACTGCAGCGGCCGCTACTAGTATTATTATGAGACCTATAAACGCAGAAAGG |
| GFP^C_F_ | TATGGTGTTCAATGCTTTGCGAG |
| GFP^C_R_H6 | GTGGTGATGGTGATGATGTTTGTATAGTTCATCCATGCCATGTG |
| NpuDnaE^C_F_T7RBS-H6 | GGAGATACTAGATGCATCATCACCATCACCACATCAAAATAGCCACACGTAAATATTTAGGC |
| NpuDnaE^C_R_GFP^C | CTCGCAAAGCATTGAACACCATAACAATTAGAAGCTATGAAGCCATTTTTGAGTG |
| pSB_F_GFP^C-H6 | AACTATACAAACATCATCACCATCACCACTAATAATACTAGTAGCGGCCGCTGC |
| pSB-T7RBS_R_H6 | GATGGTGATGATGCATCTAGTATCTCCTTCTTAAAGTTAAACAAAATTATTC |
| GFP^N_F_T7RBS-H6 | GGAGATACTAGATGCATCATCACCATCACCACCGTAAAGGAGAAGAACTTTTCACTGGA |
| GFP^N_R_NpuDnaE^N | CAAAGTAGTGACAAGTGTTGGCCATG |
| NpuDnaE^N_F_GFP^N | TGGCCAACACTTGTCACTACTTTGTGTTTAAGCTATGAAACGGAAATATTGAC |
| NpuDnaE^N_R_H6 | GGTGATGATGATTCGGCAAATTATCAACCCGCATC |
| pSB_F_NpuDnaE^N-H6 | GATAATTTGCCGAATCATCATCACCATCACCACTAATAATACTAGTAGCGGCCGCTGC |
| NpuDnaE^C_R_SN->AG-sfGFP^C | GCAAAGCACTGAACACCATAGCAACCAGCAGCTATGAAGCCATTTTTGAGTGC |
| NpuDnaE^C_F_H6 | CATCATCATATCAAAATAGCCACACGTAAATATTTAGGC |
| NpuDnaE^C_R_sfGFP | GCAAAGCACTGAACACCATAGCAATTAGAAGCTATGAAGCCATTTTTGAGTG |
| sfGFP^C_F_NpuDnaE^C | GCTATGGTGTTCAGTGCTTTGCTC |
| sfGFP^C_R_H6 | GTGGTGGTGGTGGTGTTTGTACAGTTCATCCATACCATGC |
| pSB_F_H6-sfGFP^C | GTACAAACACCACCACCACCACCACTAATAATACTAGTAGCGGCCGCTGC |
| pSB-T7RBS_R_NpuDnaE^C-H6 | CGTGTGGCTATTTTGATATGATGATGATGATGATGCATCTAGTATCTCCTTCTTAAAGTTAAACAAAATTATTC |
| NpuDnaE^N_F_sfGFP^N | GACGCTGTGTTTAAGCTATGAAACGGAAATATTGACAGTAG |
| NpuDnaE^N_R_H6 | GATGATTCGGCAAATTATCAACCCGCATC |
| sfGFP^N_F_T7RBS-H6 | GAGATACTAGATGCACCACCACCACCACCACCGTAAAGGCGAAGAGCTGTTC |
| sfGFP^N_R_NpuDnaE^N | CGTTTCATAGCTTAAACACAGCGTCGTTACCAGAGTCG |
| pSB_F_NpuDnaE^N-H6 | GGGTTGATAATTTGCCGAATCATCATCATCATCATCATTAATAATACTAGTAGCGGCCGCTGCAG |
| pSB-T7RBS_R_H6 | GTGGTGGTGGTGCATCTAGTATCTCCTTCTTAAAGTTAAACAAAATTATTC |
| sfGFP^N_R_C->G-NpuDnaE^N | CATAGCTTAAACCCAGCGTCGTTACCAGAGTCG |
| NpuDnaE^N_F_sfGFP-C>-G | CGACGCTGGGTTTAAGCTATGAAACGGAAATATTGACAGTAG |
| pSBX1C3-Ter_F_KpnI | CTAGGGTCTACGTGATCGAGGTACCTACTAGAGCCAGGCATCAAATAAAA |
| pSBX1C3-GFP_R_Ext_IntN_BamHI | GATCGAGGATCCCGCCAGAGAGATCAGAGAGTCACCAGAGATGCAACCAGATTCACGCAGTTTGTATAGTTCATCCATGCCATG |
| BamHI-MTS_bs_minD-KpnI-**_F | GATCCCCTTTACAGGTGCTTGAAGAGCAAAACAAAGGAATGATGGCTAAGATTAAGTCATTTTTCGGAGTAAGATCTTAATAAGGTAC |
| BamHI-MTS_bs_minD-KpnI-**_R | CTTATTAAGATCTTACTCCGAAAAATGACTTAATCTTAGCCATCATTCCTTTGTTTTGCTCTTCAAGCACCTGTAAAGGG |
| SspDnaBC_F_M+H6 | GCCGCTTCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATACTAGATGCATCATCACCATCACCACAGCACAGGAAAAAGAGTTTCTATTAAAGATT |
| SspDnaBC_R_N->A+SGG | GCCGGACTGCAGCGGCCGCTACTAGTATTATTACCCGCCTGAGGCATGGACAATGATGTCATTGGC |
| SspDnaBC_F_M-noH6 | GCCGCTTCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATACTAGATGAGCACAGGAAAAAGAGTTTCTATTAAAGATT |
| CPEC-GFP_F_MTS | GCAAAACAAAGGAATGATGGCTAAGATTAAGTCATTTTTCGGAGTAAGATCTAATACTAGAGCCAGGCATCAAATAAAACGAAAG |
| CPEC-GFP_R_Next_Nint | GCCAGAGAGATCAGAGAGTCACCAGAGATGCAACCAGATTCACGCAGTTATTATTTGTATAGTTCATCCATGCCATGTGTAATC |
| CPEC_GFP_Insert_oligo_F | CTCTGGTGACTCTCTGATCTCTCTGGCGCCTTTACAGGTGCTTGAAGAGCAAAACAAAGGAATGATGGCTAAGATTAAGT |
| dnmt1insert_F_BsaI | ATGTTCGGTCTCAGGGTGGCGGTGGCAAAGATCGCATTAGCTGGCTGGGTCAG |
| dnmt1insert_G_BsaI | ATGTTCGGTCTCATGCGCTGCTCAGCAGACACAGTTTAATTTC |
| mRFP_F_BsmBIBsaI | ACTTACGGTCTCACGCAAGAGACGCAATACGCAAACCGCCTCTC |
| mRFP_R_BsmBIBsaI | GCTTACGGTCTCAGAAGAGAGACGTATAAACGCAGAAAGGCCCACC |
| LambdaLys_R_BsaI | CTGGTCTCTTACATCAATCTCTCTGACCGTTCCG |
| ccdB_F_BsmBI-BsaI | CTCGTCTCATGTAAGAGACCACTGGCTGTGTATAAGGGAGCCTGAC |
| ccdB_R_BsaI-BsmBI | CTCGTCTCAAGCAAGAGACCCGCGTGGATCCGGCTTACTAAAAG |
| CPEC_GFP_Insert_oligo_R | ACTTAATCTTAGCCATCATTCCTTTGTTTTGCTCTTCAAGCACCTGTAAAGGCGCCAGAGAGATCAGAGAGTCACCAGAG |
| Smt3_F_p2Zv2Npu_flag | GATGGACTACAAAGACGATGACGACAAGTCGGACTCAGAAGTCAATCAAGAAGC |
| Smt3_R_p2Zv2Npu | GGCTATTTTGATGGATCCACCAATCTGTTCTCTGTGAG |
| p2Zv2Npu_F_Smt3 | GGTGGATCCATCAAAATAGCCACACGTAAATATTTAGGC |
| p2Zv2Npu_R_flag_Smt3 | CTTGTCGTCATCGTCTTTGTAGTCCATCTAGTATCTCCTTCTTAAAGTTAAACAAAATTATTCTC |
| h6-linker_F | TGTAGGCAGCCATCATCATCATCATCACAGCGGCCGTGGTAAA |
| h6-linker_R | AGCATTTACCACGGCCGCTGTGATGATGATGATGATGGCTGCC |
| flex-linker_F | TGTAGGTGGTTCTGGTGGTGGTTCTGGACGTGGTAAA |
| flex-linker_R | AGCATTTACCACGTCCAGAACCACCACCAGAACCACC |
| rigid-linker_F | TGTAGGTGGCGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGCAGCACCGCGTGGTAAA |
| rigid-linker_R | AGCATTTACCACGCGGTGCTGCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGCGCCACC |
| ccdB-pSBX4C5-mRFP(N)_F | AGAGACGACTGGCTGTGTATAAGGG |
| ccdB-pSBX4C5-mRFP-(N)_R | TGAGACCTATAAACGCAGAAAGGC |
| mRFP-pSBX4C5-ccdB-(C)_F | AGAGACCCAATACGCAAACCG |
| mRFP-pSBX4C5-ccdB-(C)_R | TGAGACGCGCGTGGATC |
| NpuDnaEN_F_C->G-BsaI-mRFP | GGCCTTTCTGCGTTTATAGGTCTCAGGCTTAAGCTATGAAACGGAAATATTGACA |
| NpuDnaEN_R BsmBI_ccdB | CCCTTATACACAGCCAGTCGTCTCTACCACCATTCGGCAAATTATCAACCCG |
| Npu DnaEC_F_BsmBI_ccdB | GATCCACGCGCGTCTCAGGTGGAATCAAAATAGCCACACGTAAATATTTAGG |
| NpuDnaEC_R SN->AG-BsaI-mRFP | CGGTTTGCGTATTGGGTCTCTGCCTGCAGCTATGAAGCCATTTTTGAGTG |
| Universal-S11N_F_BsaI_mRFP | GGCCTTTCTGCGTTTATAGGTCTC |
| Universal-S11N_R BsmBI_ccdB | CCCTTATACACAGCCAGTCGTC |
| Ssp DnaX-S11N_F_C->G_BsaI_mRFP | GGCCTTTCTGCGTTTATAGGTCTCAGGCTTAACCGGCGACAGCC |
| Ssp DnaX-S11C_F_BsmBI_ccdB | GATCCACGCGCGTCTCAGGTGGTGGCCTGCTGGTA |
| Ssp DnaX-S11C_R_BsaI_mRFP | CGGTTTGCGTATTGGGTCTCTGTTGTGTACCAGCAGGCCACCACC |
| Ssp DnaX-S11C_R | CGGTTTGCGTATTGGGTCTCTGCCTGCTACCAGCAGGCCACCACC |
| Ssp GyrB-S11N_F_C->G_BsaI_mRFP | GGCCTTTCTGCGTTTATAGGTCTCAGGCTTCAGCGGTGACACCCTG |
| Ssp GyrB-S11C_F_BsmBI_ccdB | GATCCACGCGCGTCTCAGGTGGTGGTGTTTTCGTT |
| Ssp GyrB-S11C_R_BsaI_mRFP | CGGTTTGCGTATTGGGTCTCTGTTGTGAACGAAAACACCACCACCTGAG |
| Ssp GyrB-S11C_R_BsaI_mRFP | CGGTTTGCGTATTGGGTCTCTGCCTGCAACGAAAACACCACCACCTGAG |
| Ter DnaE3-S11N_F_C->G_BsaI_mRFP | GGCCTTTCTGCGTTTATAGGTCTCAGGCTTAACTTACGAAACTGAGATCATGACC |
| Ter DnaE3-S11C_F_BsmBI_ccdB | GATCCACGCGCGTCTCAGGTGGTGGTCTGATCGCG |
| Ter DnaE3-S11C_R_BsaI_mRFP | CGGTTTGCGTATTGGGTCTCTGTTGCTCGCGATCAGACCACCACC |
| Ter DnaE3-S11C_R | CGGTTTGCGTATTGGGTCTCTGCCTGCCGCGATCAGACCACCACC |
| dnmt1insert_R_Flag_BsaI | GGTCTCAGAAGCTTGTCGTCATCGTCTTTGTAGTCTGCGCTGCTCAGCAGACACAG |
| DNMT1_Substrate_R_1stMedC | GAAGCTGGGACTTCCGGGAGCTTC[5MedC]GATCTGAGAGTGCAA |
| DNMT1_Substrate_F_1stMedC | TTGCACTCTCAGAT[5MedC]GGAAGCTCCCGGGAAGTCCCAGCTTC |
| DNMT1_Substrate_F_2MedC | TTGCACTCTCAGAT[5MedC]GGAAGCTCC[5MedC]GGGAAGTCCCAGCTTC |
| DNMT1_Substrate_F | TTGCACTCTCAGATCGGAAGCTCCCGGGAAGTCCCAGCTTC |
| DNMT1_Substrate_R | GAAGCTGGGACTTCCGGGAGCTTCCGATCTGAGAGTGCAA |
| DNMT1_Substrate_R_2MedC | GAAGCTGGGACTTC[5MedC]GGGAGCTTC[5MedC]GATCTGAGAGTGCAA |
| VF2-C7T | TGCCACTTGACGTCTAAGAA |
| sfGFP_T65C_F | CGACGCTGTGTTATGGTGTTCAGTGCTTTGCTC |
| sfGFP_T65C_R | GAACACCATAACACAGCGTCGTTACCAGAGTCG |
| rfp_F_BsaI | ACTTAACGCAAGAGACCCAATACGCAAACCGCCTCTCC |
| rfp_R_BsaI | ACTTTCGAAGTGAGACCTATAAACGCAGAAAGGCCCACCC |
| LiLaLy_F_BsaI | CTGGTCTCATAATAAATCGGTGAAATGCACGACTG |
| LiLaLy_R_IasB | CTGGTCTCTTACATCAATCTCTCTGACCGTTCCG |
| H6+exteins-insert_F | TGTAGGCAGCCATCATCATCATCATCACAGCGGCCGTGGTAAATGCTGGGAA |
| H6+exteins-insert_R | ATTATTCCCAGCATTTACCACGGCCGCTGTGATGATGATGATGATGGCTGCC |
| XylaLin_F_BsmBI | CTCGTCTCATAATAATACTAGTAGCGGCCGCTG |
| XylaLin_R_IBmsB | CTCGTCTCTCCACACTGTTACGTTAGAACTTCCAC |
| H6insert(Xyla)_F | GTGGGGCAGCCATCACCATCACCATCATAGTGGT |
| H6insert(Xyla)_R | ATTAACCACTATGATGGTGATGGTGATGGCTGCC |
| H6insert+exteins(Xyla)_F | GTGGCATCACCATCACCATCATCGTGGTAAATGCTGGGAA |
| H6insert+exteins(Xyla)_R | ATTATTCCCAGCATTTACCACGATGATGGTGATGGTGATG |
| H6Smt3_F_BBprefix | CGCTTCTAGATGGGCAGCAGCCATCATC |
| mRFPexp_F_H6-TEVc | CACAGCAGCGGCGAAAACCTGTACTTCCAGGGTAGAGACCAATACGCAAACCGCCTCTC |
| mRFPexp_F_Smt3-TEVc-BsaI | GTGGATCCGAAAACCTGTACTTCCAGGGTAGAGACCAATACGCAAACCGCCTCTC |
| mRFPexp_R_BBsuffix-mockSortAc | GCTACTAGTATTATTAGGTTTCCGGAAGTGAGACCTATAAACGCAGAAAGGCCCACC |
| pSB**3_F_mockSortAc | GGTCTCACTTCCGGAAACCTAATAATACTAGTAGCGGCCGCTGCAGTCC |
| pSB**3_R_Smt3 | GCTGCTGCCCATCTAGAAGCGGCCGCGAATTC |
| pSB**3_R_TEVc-H6 | GGTTTTCGCCGCTGCTGTGATGATGATGATGATGACTACTGCCCATCTAGAAGCGGCCGCGAATTC |
| Smt3_R_TEVc-BsaI | CTGGAAGTACAGGTTTTCGGATCCACCAATCTGTTCTCTGTGAG |
| mRFPexp_F_TEVc-BsaI | CAGTAGTGAGAATCTTTACTTCCAGGGTTGAGACCAATACGCAAACCGCCTCTCC |
| mRFPexp_R_BBsuffix-H6-SortAc | CTAGTATTATTAGTGATGGTGATGGTGATGACCACCAGTTTCAGGAAGTGAGACCTATAAACGCAGAAAGGCCCACC |
| pSB**3_R_TEVc-BsaI | CAACCCTGGAAGTAAAGATTCTCACTACTGCCCATCTAGAAGCGGCCGCGAATTCC |
| eGFP_F_BsaI-G5RM | CAGAACGGTCTCAGGGTGGAGGAGGAGGAAGAATGGTGAGCAAGGGCGAGGAGC |
| eGFP_R_BsaI | CAGAACGGTCTCAGAAGCTTGTACAGCTCGTCCATGCC |
| ccdB_F_BsaI-BsmBI | CAGAACGGTCTCACGCATGAGACGACTGGCTGTGTATAAGGGAGCCTGAC |
| ccdB_R_BsaI-BsmBI | CAGAACGGTCTCAGAAGTGAGACGCGCGTGGATCCGGCTTAC |
| DNMT1lnk_0901/1f-FLAG_F | CGCAGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTGATTATAAAGATGATGATGATAAA |
| DNMT1lnk_0901/1f-FLAG_R | CCAGTTTATCATCATCATCTTTATAATCACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACC |
| DNMT1lnk_0825/1r-FLAG-p1F | CGCAGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGAAGCAGCAGCAA |
| DNMT1lnk_0825/1r-FLAG-p1R | TTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGC |
| DNMT1lnk_0825/1r-FLAG-p2F | AAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGCAGCAGCAAGCGGAGCAGCAGCAGAAGCAGCAGCAAAAGATTATAAAGATGATGATGATAAA |
| DNMT1lnk_0825/1r-FLAG-p2R | CCAGTTTATCATCATCATCTTTATAATCTTTTGCTGCTGCTTCTGCTGCTGCTCCGCTTGCTGCTGCTTTTGCTGCTGCTTCTTTTGCTGCTGC |
| EX-BsaI-A-NN-IasB-S_F | AATTCGCGGCCGCTTCTAGAGGTCTCCGATGCCTGCCTGCTAGAGACCTGTCTA |
| EX-BsaI-A-NN-IasB-S_R | CTAGTAGACAGGTCTCTAGCAGGCAGGCATCGGAGACCTCTAGAAGCGGCCGCG |
| EX-BsaI-CC-O-IasB-S_F | AATTCGCGGCCGCTTCTAGAGGTCTCCCAACCCTGCCTAATAGAGACCTGTCTA |
| EX-BsaI-CC-O-IasB-S_R | CTAGTAGACAGGTCTCTATTAGGCAGGGTTGGGAGACCTCTAGAAGCGGCCGCG |
| SsrA-Ctag-BsaI-Cext_BsaI_F | CAACTGCTGGGAAGGAGGAGGTGCTGCTAACGACGAAAACTACGCTCTGGCTGCT |
| SsrA-Ctag-BsaI-Cext_BsaI_R | ATTAAGCAGCCAGAGCGTAGTTTTCGTCGTTAGCAGCACCTCCTCCTTCCCAGCA |
| MTS_bs_minD_C_term_F | CAACTGCTGGGAAGGAGGAGGTCCTTTACAGGTGCTTGAAGAGCAAAACAAAGGAATGATGGCTAAGATTAAGTCATTTTTCGGAGTAAGATCT |
| MTS_bs_minD_C_term_R | ATTAAGATCTTACTCCGAAAAATGACTTAATCTTAGCCATCATTCCTTTGTTTTGCTCTTCAAGCACCTGTAAAGGACCTCCTCCTTCCCAGCA |
| myristoilation-Ntag_F | GATGGGCATCGGCAAGTCCAAGGGCGGAGGCAGGGGCAAG |
| myristoilation-Ntag_R | AGCACTTGCCCCTGCCTCCGCCCTTGGACTTGCCGATGCC |
| NLS-Ntag_F | GATGCCCAAGAAAAAGCGCAAGGTAGGCGGAGGCAGGGGCAAG |
| NLS-Ntag_R | AGCACTTGCCCCTGCCTCCGCCTACCTTGCGCTTTTTCTTGGG |
| OmpA_linker-BsaI_F | GGCCTTGGTCTCAGATGCATATGAAAGCTACTAAACTGGTACTGG |
| OmpA_linker-BsaI_R | GGCCTTGGTCTCGAGCACTTGCCCCTAGAGCTCCCTCCTCCAGAAC |
| pel_B-Ntag_F | GATGAAATACCTGCTGCCGACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCCGGCGGAGGCAGGGGCAAG |
| pel_B-Ntag_R | AGCACTTGCCCCTGCCTCCGCCGGCCATCGCCGGCTGGGCAGCGAGGAGCAGCAGACCAGCAGCAGCGGTCGGCAGCAGGTATTT |
| PKI_NES-Ntag_F | GATGTTGGCTTTGAAATTGGCTGGTTTGGATATTGGCGGAGGCAGGGGCAAG |
| PKI_NES-Ntag_R | AGCACTTGCCCCTGCCTCCGCCAATATCCAAACCAGCCAATTTCAAAGCCAA |
| DNMT1_F_BsaI=CAAC-CWE | ATGTTCGGTCTCACAACTGCTGGGAAAAAGATCGCATTAGCTGGCTGGGTCAG |
| ccdB_R_BsmBI=ATCA-H5SGRGK-TGCT=BsaI | CAGAACGGTCTCAAGCATTTACCACGGCCGCTGTGATGATGATGATGATTGAGACGCGCGTGGATCCGGCTTAC |
| DMMT1_R_H6SGRGT-TGCT=BsaI | CAGAACGGTCTCAAGCAGGTACCACGGCCGCTGTGATGATGATGATGATGTGCGCTGCTCAGCAGACACAG |
| SSpDnaBC_F_ccdB-CN | ATCCACGCGCGTCTCAGGTGGTAGCACAGGAAAAAGAGTTTCTATTAAAG |
| SSpDnaBC_R_CC-mRFP | CGGTTTGCGTATTGGGTCTCTGTTGTGGACAATGATGTCATTGGC |
| SSpDnaBC_R_CC*-mRFP | CGGTTTGCGTATTGGGTCTCTGCCTGCGACAATGATGTCATTGGC |
| SspDnaBN_F_mRFP-NN | GCCTTTCTGCGTTTATAGGTCTCATGCTTATCTGGTGACTCTCTGATCTCTCTGG |
| SspDnaBN_F_mRFP-NN* | GCCTTTCTGCGTTTATAGGTCTCAGGCTTATCTGGTGACTCTCTGATCTCTCTGG |
| SspDnaBN_R_NC-ccdB | CCTTATACACAGCCAGTCGTCTCTACCACCACGGGACGCCAGAGAGATCAGAGAGTCACCAG |
| CBD_His_BsaI_f | ATGTATGGTCTCAGATGCATCATCACCATCACCACGGTGGAGGTACCACAAATCCTGGTGTATC |
| CBD_Nextein_BsaI_r | AAGTTCGGTCTCGAGCCACCAGATTCACGCAGTTGAAGCTGCCACAAGGC |
| DNMT1_F_BsaI=GGGT-G | ATGTTCGGTCTCAGGGTGGTAAAGATCGCATTAGCTGGCTGGGTCAG |
| AsLOV2-mutagenesis_middle_PstI_F | GAAACTGTAGGTTTCTACAAGGTCCTGAAAC |
| AsLOV2_mutagenesis_middle_PstI_R | AGGACCTTGTAGAAACCTACAGTTTCTTCC |
| ccdB_R_BsmBI=ATCA-H5SGRGT-TGCT=BsaI | CAGAACGGTCTCAAGCAGGTACCACGGCCGCTGTGATGATGATGATGATTGAGACGCGCGTGGATCCGGCTTAC |
| DNMT1lnk_0901/1f-FLAG_R_v2 | GAAGTTTATCATCATCATCTTTATAATCACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACC |
| DNMT1lnk_0915/4r-FLAG_p2F | AAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGCAGCAAAAACCGCAGCAGCAGAAGCAGCAGCAAAAGATTATAAAGATGATGATGATAAA |
| DNMT1lnk_0915/3f_p1F | CGCAGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGT |
| DNMT1lnk_0915/3f_p1R | GCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACC |
| DNMT1lnk_0915/3f_p2F | GGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCC |
| DNMT1lnk_0915/3f_p2R | TGATGGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACC |
| rigid+exteins-insert_F | TGTAGGTGGCGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGCAGCACCGCGTGGTAAATGCTGGGAA |
| rigid+exteins-insert_R | ATTATTCCCAGCATTTACCACGCGGTGCTGCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGCGCCACC |
| DNMT1lnk_0915/4r-FLAG_p2R | GAAGTTTATCATCATCATCTTTATAATCTTTTGCTGCTGCTTCTGCTGCTGCGGTTTTTGCTGCTTTTGCTGCTGCTTCTTTTGCTGCTGC |
| DNMT1lnk_0915/2r_p1F | CGCAGCGGAAGCGGCGGCGAAAAACGTGCTGGCGGAAGCGGCGGCGAAAGAAGCGGCGGCGAAAGAAGCGGCGGCGA |
| DNMT1lnk_0915/2r_p1R | TTCTTTCGCCGCCGCTTCTTTCGCCGCCGCTTCTTTCGCCGCCGCTTCCGCCAGCACGTTTTTCGCCGCCGCTTCCGC |
| DNMT1lnk_0915/2r_p2F | AAGAAGCGGCGGCGAAAGAAGCGGCGGCGAAAGCGAACGTGCTGGCGGAAGCGGCGGCGAAAGAAGCGGCGGCGAAAC |
| DNMT1lnk_0915/2r_p2R | TGATGTTTCGCCGCCGCTTCTTTCGCCGCCGCTTCCGCCAGCACGTTCGCTTTCGCCGCCGCTTCTTTCGCCGCCGC |
| DNMT1lnk_0915/6f_p1F | CGCAGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGG |
| DNMT1lnk_0915/6f_p1R | GCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACC |
| DNMT1lnk_0915/6f_p2F | TAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGATTATAAAGATGATGATGATAAA |
| DNMT1lnk_0915/6f_p2R | GAAGTTTATCATCATCATCTTTATAATCGCTACCACCACCGCTACCACCACCGCTACCACCACC |
| DNMT1lnk_0915/1r_p1F | CGCAGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGCAAAC |
| DNMT1lnk_0915/1r_p1R | GCACGTTTGCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGC |
| DNMT1lnk_0915/1r_p2F | GTGCTGGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGAAGCAGCAGCAA |
| DNMT1lnk_0915/1r_p2R | TTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGCCA |
| DNMT1lnk_0915/1r_p3F | AAGAAGCAGCAGCAAAAGCTAACGTGCTGGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAA |
| DNMT1lnk_0915/1r_p3R | GAAGTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGCCAGCACGTTAGCTTTTGCTGCTGC |
| Backbone-H6_R | GATCCGTGGTGGTGGTGGTGG |
| H6-Backbone_F | GGTGGTCATCATCATCATCATCATTAATAATACTAGTAG |
| LOV-6_R_SspDnaXC | GCAGTTGTGTACCAGCAGGCCTTCTTTCGCCGCTTCATCAATATTTTGTGCAGTTTTCTTAATCAGCATGAC |
| LOV-12_R_BsaIIasB | CGAGACCACCTTATTGGTCTCCAATATTTTGTGCAGTTTTCTTAATCAGCATGAC |
| LOV-12_R_SspDnaXC | GCAGTTGTGTACCAGCAGGCCAATATTTTGTGCAGTTTTCTTAATCAGCATGAC |
| sfGFPC_F_BsaIIasB | GGAGACCAATAAGGTGGTCTCGTGCTATGGTGTTCAGTGCTTTGC |
| sfGFPC_F_SspDnaXC | GCCTGCTGGTACACAACTGCTATGGTGTTCAGTGCTTTGC |
| sfGFPC_R_H6 | ATGATGATGATGATGATGACCACCTTTGTACAGTTCATCCATACCATGCG |
| sfGFPN_F_H6 | CACCACCACCACGGATCACGTAAAGGCGAAGAGCTGTTC |
| sfGFPN_R_SspDnaXN | CTGGCTGTCGCCGGTTAAGCACAGCGTCGTTACCAGAG |
| sfGFPN_R_SspDnaXN* | CTGGCTGTCGCCGGTTAAACCCAGCGTCGTTACCAG |
| SspDnaXN_F | TTAACCGGCGACAGCC |
| SspDnaXC*-sfGFP_F_H6 | CACCACCACCACGGATCAGGCCTGCTGGTAGCTGGTTGCTATGGTGTTCAGTGCTTTGC |
| SspDnaXC-sfGFP_F_H6 | CACCACCACCACGGATCAGGCCTGCTGGTACACAACTGCTATGGTGTTCAGTGCTTTGC |
| SspDnaXN_R_H6 | ATGATGATGATGATGATGACCACCGTTTGCAACGAAG |
| mRFP2-168_F_H6 | CACCACCACCACGGATCAGCTTCCTCCGAAGACGTTATC |
| mRFP2-168_R_SspDnaXN | CTGGCTGTCGCCGGTTAAGCATTCGTCGATACCAGATCCTTTCAGTTTCAGACGCATTTTG |
| mRFP2-168_R_SspDnaXN* | CTGGCTGTCGCCGGTTAAACCTTCGTCGATACCAGATCCTTTCAGTTTCAGACGCATTTTG |
| mRFP169-225_F_-6-SspDnaXC-Ext | GCCTGCTGGTACACAACTGCCACACCGACGGTGGTCACTACGACGC |
| mRFP169-225_F_-12-SspDnaXC-Ext-Jalpha | GCCTGCTGGTACACAACTGCCACACCGCGAACCTGGACGGTGGTCACTACGACGC |
| mRFP169-225_F_BsaIIasB | GGAGACCAATAAGGTGGTCTCGGGTGGTCACTACGACGCTGAAG |
| mRFP169-225_F_H6-SspDnaXC | CACCACCACCACGGATCAGGCCTGCTGGTACACAACTGCCACACCGACGGTGGTCACTACGACGC |
| mRFP169-255_F_H6-SspDnaXC* | CACCACCACCACGGATCAGGCCTGCTGGTAGCTGGTTGCCACACCGACGGTGGTCACTACGACGC |
| mRFP169-255_R_H6 | ATGATGATGATGATGATGACCACCAGCACCGGTGGAGTGACG |
| LOV_F_H6 | CACCACCACCACGGATCATTGGCTACTACACTTGAACGTATTG |
| GFPc*158-238_F_NWC_CAAC_BsaI | GCATGGTCTCAAGGCTGCTGGAACCAAAAGAATGGAATCAAAGTTAACTTC |
| GFPc*_R_H6_TAAT=IasB | CGTAGGTCTCTATTAGTGGTGATGGTGATGATGTTTGTATAGTTCATCCATGCCATG |
| GFPn*1-157_R_KGG_GGCT=IasB | CGTAGGTCTCGAGCCCTTACCTCCTTTGTCTGCCATGATGTATACATTG |
| GFPn*_F_H6_GATG_BsaI | GCATGGTCTCAGATGCATCATCACCATCACCACCGTAAAGGAGAAGAACTTTTCACTG |
| mCherryC*169-236_F_BsaI=AGGC-NWC | GCATGGTCTCAAGGCTGCTGGAACAGGCTGAAGCTGAAGGACG |
| mCherryC*_R_H6-TAAT=IasB | CGTAGGTCTCTATTAGTGGTGATGGTGATGATGCTTGTACAGCTCGTCCATGC |
| mCherryN*1-168_R_KGG-GGCT=IasB | CGTAGGTCTCAAGCCCTTACCTCCCTGCTTGATCTCGCCCTT |
| mCherryN*_F_BsaI=GATG-H6 | GCATGGTCTCAGATGCATCATCACCATCACCACGTGAGCAAGGGCGAGG |
| mRFPc*155-225_F_BsaI=AGGC-NWC | GCATGGTCTCAAGGCTGCTGGAACGGTGCTCTGAAAGGTGAAATC |
| mRFPc*169-225_F_BsaI=AGGC-NWC | GCATGGTCTCAAGGCTGCTGGAACGACGGTGGTCACTACGACG |
| mRFPc*_R_H6_TAAT=IasB | CGTAGGTCTCTATTAGTGGTGATGGTGATGATGAGCACCGGTGGAGTG |
| mRFPn*1-154_R_KGG-GGCT=IasB | CGTAGGTCTCAAGCCCTTACCTCCGTCTTCCGGGTACATACGTTC |
| mRFPn*1-168_R_KGG-GGCT=IasB | CGTAGGTCTCAAGCCCTTACCTCCTTTCAGTTTCAGACGCATTTTG |
| mRFPn*_F_H6_BsaI=GATG | GCATGGTCTCAGATGCATCATCACCATCACCACGCTTCCTCCGAAGACGTTATC |
| mRFP_F_BsmBI=GATG=IasB | AATTATCGTCTCAGATGAGAGACCCAATACGCA |
| mRFP_R_BsaI=GGTG=IBmsB | TAATATCGTCTCACACCTGAGACCTATAAACGCAG |
| mRFP_F_BsmBI=TGGT=IasB | AATTATCGTCTCATGGTAGAGACCCAATACGCAA |
| mRFP_R_BsaI=TAAT=IBmsB | TAATATCGTCTCAATTATGAGACCTATAAACGCAGAAAGG |
| bad1_F1 | TGTAGGTGGTGCAGAAGCAGCTGCTAAAGAAGCAGCAGCTAAAGTTAACCTGACCGCTGCTGAAGCT |
| bad1_R1 | TGCTGCAGCTTCAGCAGCGGTCAGGTTAACTTTAGCTGCTGCTTCTTTAGCAGCTGCTTCTGCACCACC |
| bad1_F2 | GCAGCAAAAGAAGCTGCAGCTAAAGTGAACCTGACCGCAGCAGAAGCTGCTGCAAAACGCGGTACC |
| bad1_R2 | AGCAGGTACCGCGTTTTGCAGCAGCTTCTGCTGCGGTCAGGTTCACTTTAGCTGCAGCTTCTTT |
| may1_F1 | TGTAGGTGGTGCAGAAGCTGCTGCTAAAGAAGCTGCTGCAAAAGCAGCAGCTGCACATCCGGAAGCTGCAGAAGCTGC |
| may1_R1 | TTTGCTGCAGCTTCTGCAGCTTCCGGATGTGCAGCTGCTGCTTTTGCAGCAGCTTCTTTAGCAGCAGCTTCTGCACCACC |
| may1_F2 | AGCAAAAGAAGCTGCAGCTAAAGCTAAAACCGCTGCTGAAGCTGCAGCAAAAGAAGCTGCAGCGAAAGCACGCGGTACC |
| may1_R2 | AGCAGGTACCGCGTGCTTTCGCTGCAGCTTCTTTTGCTGCAGCTTCAGCAGCGGTTTTAGCTTTAGCTGCAGCTTCT |
| sgt1_F | TGTAGGTGGTGCAGAAGCAGCTGCTAAAGAAGCTGCTGCAAAACGCGGTACC |
| sgt1_R | AGCAGGTACCGCGTTTTGCAGCAGCTTCTTTAGCAGCTGCTTCTGCACCACC |
| sgt2_F | TGTAGGTGGTGCTGAAGCTGCAGCAAAAGCTGCAGCACATCCGGAAGCTGCAGAAGCTGCAGCTAAACGCGGTACC |
| sgt2_R | AGCAGGTACCGCGTTTAGCTGCAGCTTCTGCAGCTTCCGGATGTGCTGCAGCTTTTGCTGCAGCTTCAGCACCACC |
| sho1_F | TGTAGGTGGTCGCGGTACC |
| sho1_R | AGCAGGTACCGCGACCACC |
| sho2_F | TGTAGGTGGTGCAGAAGCAGCTGCTAAACGCGGTACC |
| sho2_R | AGCAGGTACCGCGTTTAGCAGCTGCTTCTGCACCACC |
| LacI_F_VR | CAGCTCACTCAAAGGCGGTAATACTTTACGGCTAGCTCAGTCCTAGG |
| LacI_R_pSB*C3 | GTTATCCCCTGATTCTGTGGATAACCTTATTACTGCCCGCTTTCCAGTC |
| pSB*C3_F | GGTTATCCACAGAATCAGGGG |
| pSB*C3_R | GTATTACCGCCTTTGAGTGAGC |
| mRFP169-225_F_GSGIDECHT | GATCTGGTATCGACGAATGCCACACCGACGGTGGTCACTACGACGC |
| mRFP169-225_F_GSGIDECHT-Jalpha | GATCTGGTATCGACGAATGCCACACCGCGAACCTGGACGGTGGTCACTACGACGC |
| mRFP2-168_R_GSGIDECHT | GGTGTGGCATTCGTCGATACCAGATCCTTTCAGTTTCAGACGCATTTTG |
| DNMT1lnk_0917/1r_p1F | CGCAGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGCAGTAAATC |
| DNMT1lnk_0917/1r_p1R | GTAAGATTTACTGCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGC |
| DNMT1lnk_0917/1r_p2F | TTACAGCAGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGAAGCAGCAGCAA |
| DNMT1lnk_0917/1r_p2R | TTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGCTGCT |
| DNMT1lnk_0917/1r_p3F | AAGAAGCAGCAGCAAAAGCTGTAAATCTTACAGCAGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAA |
| DNMT1lnk_0917/1r_p3R | GAAGTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGCTGCTGTAAGATTTACAGCTTTTGCTGCTGC |
| DNMT1lnk_0917/2r_p1F | CGCAGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGTAAATCTTACAGCAGCAGAAG |
| DNMT1lnk_0917/2r_p1R | GCTGCTTCTGCTGCTGTAAGATTTACTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGC |
| DNMT1lnk_0917/2r_p2F | CAGCAGCAAAAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGAAGCAGCAGCAAA |
| DNMT1lnk_0917/2r_p2R | TTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTTTTGCT |
| DNMT1lnk_0917/2r_p3F | AGAAGCAGCAGCAAAAGCAGTAAATCTTACAGCAGCAGAAGCAGCAGCAAAAGCAC |
| DNMT1lnk_0917/2r_p3R | TGATGTGCTTTTGCTGCTGCTTCTGCTGCTGTAAGATTTACTGCTTTTGCTGCTGC |
| mRFPexp_R_BsmBI=ATCA-H5SGRGT-TGCT=BsaI | CAGAACGGTCTCAAGCAGGTACCACGGCCGCTGTGATGATGATGATGATGGAGACGTATAAACGCAGAAAGGCCCACC |
| mut-noPstIT-3A-GateNpuDnaE(C)SN->AG_F | GGCTTCATAGCAGCAGGCAGAGACCCAATAC |
| mut-noPstIT-3A-GateNpuDnaE(C)SN->AG_R | CTGCCTGCTGCTATGAAGCCATTTTTGAGTG |
| SspDnaE(N)_F_mRFP-NN | GCCTTTCTGCGTTTATAGGTCTCATGCTTAAGTTTTGGTACCGAAATTTTAACC |
| SspDnaE(N)_R_NC-ccdB | CCTTATACACAGCCAGTCGTCTCTACCACCACGGGATTTAATTGTACCTGCGTCAAGTAATG |
| SSpDnaE(C)_F_ccdB-CN | ATCCACGCGCGTCTCAGGTGGTGTTAAAGTTATCGGTCGTCGATC |
| SSpDnaE(C)_R_CC-mRFP | CGGTTTGCGTATTGGGTCTCTGTTGGCGGCGATAGC |
| univ#1long_F | TGTAGGTGGTGCAGAAGCAGCAGCTAAAGAAGCT |
| univ#1long_R | AGCTGCAGCTTCTTTAGCTGCTGCTTCTGCACCACC |
| univ#1short_F | TGTAGGTGGTGCAGAAGCTGCT |
| univ#1short_R | CTTTGCAGCAGCTTCTGCACCACC |
| univ#3_F | GCTGAAGCAGCTGCTAAAGAAGCAGCAGCTAAAGAAGCTGCTGCTAAAGAAGCTGCAGCTAAAGAAGCAGCTGCT |
| univ#3_R | TGCCTTAGCAGCTGCTTCTTTAGCTGCAGCTTCTTTAGCAGCAGCTTCTTTAGCTGCTGCTTCTTTAGCAGCTGC |
| bad2#2_F | GCAAAGGTGAACCTGACAGCT |
| bad2#2_R | TTCAGCAGCTGTCAGGTTCAC |
| bad2#4_F | AAGGCAAAAACCGCAGCTGAAGCTGCTGCAAAAGAAGCTGCAGCTAAAGAAGCTGCTGCAAAAGCTCGTGGTACC |
| bad2#4_R | AGCAGGTACCACGAGCTTTTGCAGCAGCTTCTTTAGCTGCAGCTTCTTTTGCAGCAGCTTCAGCTGCGGTTTT |
| bad3#2_F | GCAAAGGCATCTCTGCCGGCAGCT |
| bad3#2_R | TTCAGCAGCTGCCGGCAGAGATGC |
| bad3#4_F | AAGGCAAACGTGCTGGCAGAAGCAGCTGCTAAAGCACGTGGTACC |
| bad3#4_R | AGCAGGTACCACGTGCTTTAGCAGCTGCTTCTGCCAGCACGTT |
| bad4#2_F | GCAGCTAAAGAAGCTGCTGCTAAAGAAGCAGCTGCTAAAGAAGCAGCAGCTAAAGCTGTGAACCTGACCGCA |
| bad4#2_R | TTCAGCTGCGGTCAGGTTCACAGCTTTAGCTGCTGCTTCTTTAGCAGCTGCTTCTTTAGCAGCAGCTTCTTT |
| bad4#4_F | AAGGCAGTGAACCTGACCGCAGCTGAAGCTGCAGCTAAAGAAGCAGCTGCTAA |
| bad4#4_R | GCTTCTTTAGCAGCTGCTTCTTTAGCTGCAGCTTCAGCTGCGGTCAGGTTCAC |
| bad4#5_F | AGAAGCAGCTGCTAAAGAAGCAGCTGCTAAAGAAGCAGCTGCTAAAGCACGTGGTACC |
| bad4#5_R | AGCAGGTACCACGTGCTTTAGCAGCTGCTTCTTTAGCAGCTGCTTCTTTAGCAGCT |
| may2#2_F | GCAAAGGCTAAAACCGCTGCTGAAGCAGCAGCTAAAGAAGCAGCTGCGAAACGTGGTACC |
| may2#2_R | AGCAGGTACCACGTTTCGCAGCTGCTTCTTTAGCTGCTGCTTCAGCAGCGGTTTTAGC |
| may3#2_F | GCAGCTAAAGCTACCGGTGATCTGGCTGCAGAAGCAGCTGCAAAAGAAGCTGCTGCAAAAGCTCGCGGTACC |
| may3#2_R | AGCAGGTACCGCGAGCTTTTGCAGCAGCTTCTTTTGCAGCTGCTTCTGCAGCCAGATCACCGGTAGCTTT |
| ord1#2_F | GCAGCTAAAGCTACCGGTGATCTGGCTGCTGAAGCAGCAGCTAAAGCTGCACGTGGTACC |
| ord1#2_R | AGCAGGTACCACGTGCAGCTTTAGCTGCTGCTTCAGCAGCCAGATCACCGGTAGCTTT |
| ord2#2_F | GCAAAGGCTGCTGCAGCTGATGGTACCCTGGCAGAAGCTGCTGCTAAAGCAGCTCGTGGTACC |
| ord2#2_R | AGCAGGTACCACGAGCTGCTTTAGCAGCAGCTTCTGCCAGGGTACCATCAGCTGCAGCAGC |
| ord3#2_F | GCAGCTAAAGCTAGCCTGCCGGCTGCTGCAGAAGCTGCTGCAAAAGAAGCTGCAGCTAAACGTGGTACC |
| ord3#2_R | AGCAGGTACCACGTTTAGCTGCAGCTTCTTTTGCAGCAGCTTCTGCAGCAGCCGGCAGGCTAGCTTT |
| ord4#2_F | GCAGCTAAAGCTACCGGTGATCTGGCAGCAGAAGCTGCAGCTAAACGTGGTACC |
| ord4#2_R | AGCAGGTACCACGTTTAGCTGCAGCTTCTGCTGCCAGATCACCGGTAGCTTT |
| ord5#2_F | GCAAAGGCTGCTACCGGTGATCTGGCAGCAGAAGCAGCTGCTAAAGCACGTGGTACC |
| ord5#2_R | AGCAGGTACCACGTGCTTTAGCAGCTGCTTCTGCTGCCAGATCACCGGTAGCAGC |
| ord6#2_F | GCAAAGGCTAGCCTGCCGGCTGCAGCTGAAGCTGCAGCGAAAGAAGCAGCAGCTAAAGCTCGCGGTACC |
| ord6#2_R | AGCAGGTACCGCGAGCTTTAGCTGCTGCTTCTTTCGCTGCAGCTTCAGCTGCAGCCGGCAGGCTAGC |
| sgt3#2_F | GCAAAGGTGAACCTGACCGCTGCTGAAGCTGCAGCTAAACGTGGTACC |
| sgt3#2_R | AGCAGGTACCACGTTTAGCTGCAGCTTCAGCAGCGGTCAGGTTCAC |
| sgt4#2_F | GCAAAGGCTGCAGCACATCCGGAAGCTGCTGAAGCAGCTGCAAAAGCACGTGGTACC |
| sgt4#2_R | AGCAGGTACCACGTGCTTTTGCAGCTGCTTCAGCAGCTTCCGGATGTGCTGCAGC |
| sho3#2_F | GCAAAGGCTACCGGTGATCTGGCAGAAGCAGCTGCTAAACGTGGTACC |
| sho3#2_R | AGCAGGTACCACGTTTAGCAGCTGCTTCTGCCAGATCACCGGTAGC |
| rigid_RGT_F | TGTAGGTGGCGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGCAGCACCGCGTGGTACC |
| rigid_RGT_R | AGCAGGTACCACGCGGTGCTGCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGCGCCACC |
| ccdB_R_BsmBI=ATCA-H5SGRGT-TGCT=BsaI | CAGAACGGTCTCAAGCAGGTACCACGGCCGCTGTGATGATGATGATGATTGAGACGCGCGTGGATCCGGCTTAC |
| ccdB_R_BsmBI=ATCA-H5SGRGT-iGEMHD | CGTGCATTTCACCGATTTATTAGGTACCACGGCCGCTGTGATGATGATGATGATTGAGACGCGCGTGGATCCGGCTTAC |
| Smt3_F_T7RBS | TTTGTTTAACTTTAAGAAGGAGATACTAGATGTCGGACTCAGAAGTCAATCAAGAAG |
| DNMT1_F_Smt3-CWE | GTGGATGCTGGGAAAAAGATCGCATTAGCTGGCTGG |
| Smt3_R_CWE-DNMT1 | TCTTTTTCCCAGCATCCACCAATCTGTTCTCTGTGAGC |
| DNMT1_R_H6SGRGT-iGEMHD | CGTGCATTTCACCGATTTATTAGGTACCACGGCCGCTGTGATGATGATGATGATGTGCGCTGCTCAGCAGACAC |
| DNMT1_R_RFC10suffix | TGCAGCGGCCGCTACTAGTATTATTATGCGCTGCTCAGCAGAC |
| DNMT1/BB1_R_DNMT1/BB2 | CATACTGACCTGCTTGCAGAACACCAAAGG |
| DNMT1/BB2_F_DNMT1/BB1 | GGTGTTCTGCAAGCAGGTCAGTATGGTGTTGC |
| DNMT1/BB2_R_DNMT1/BB3 | CATCATGAAAGGTATATTGCAGTTTATGTGCAATAAC |
| DNMT1/BB3_F_DNMT1/BB2 | GCACATAAACTGCAATATACCTTTCATGATGTG |
| pSB**3_F_TAATAA | TAATAATACTAGTAGCGGCCGCTGCAGTCC |
| Cas9CtrlSplit1_Fw | TGCTTCAACGGCGATAGCCTGCACGAG |
| Cas9CtrlSplit1_Re | GTTGAAGCAGGACACCTGGGCTTTCTGG |
| Cas9CtrlSplit2_Fw | GCCAGAGAGTACTGCTTCAACCAGACCACCCAGAAGG |
| Cas9CtrlSplit2_Re | CTGGTTGAAGCAGTACTCTCTGGCCATTTCGATC |
| Cas9Split1C_F_CFN_NpuDnaEC | CAACTGCTTCAACGGCGATAGTCTGCACGAGCAC |
| Cas9Split1N_R_NpuDnaEN | CATAGCTCAAGCAGGACACCTGGGCTTTCTGGATG |
| Cas9Split2C_F_CF_NpuDnaEC | CCAACTGCTTCAACCAGACCACCCAGAAGGG |
| Cas9Split2N_R_Y_NpuDnaEN | CATAGCTCAAGCAGTACTCTCTGGCCATTTCGATC |
| mCherry_Fw_BamHI | ATCGCGGATCCGGCACCATGGTGAGCAAGGGCGAGGAG |
| mCherry_Rv_HindIII | GCGATAAGCTTCTACTTGTACAGCTCGTCCATGCCGC |
| NpuDnaECSplit1&2C_F_NLS_Flag | AGACGATGACGATAAGCCAAAGAAGAAGCGGAAGGTCGGTGGTATCAAAATAGCCACACGTAAATATTTAGGC |
| NpuDnaECSplit1C_R_CFN_Cas9Split1C | CTATCGCCGTTGAAGCAGTTGGAAGCTATGAAGCCATTTTTG |
| NpuDnaECSplit2C_R_CF_Cas9Split2C | GTGGTCTGGTTGAAGCAGTTGGAAGCTATGAAGCCATTTTTG |
| NpuDnaENSplit1&2N_R_NLS | CTCCGTGGATACCGACCTTCCGCTTCTTCTTTGGGGCACCACCATTCGGCAAATTATCAACCC |
| NpuDnaENSplit1N_F_Cas9Split1N | CCAGGTGTCCTGCTTGAGCTATGAAACGG |
| NpuDnaENSplit2N_Fw_Y_Cas9Split2N | GCCAGAGAGTACTGCTTGAGCTATGAAACGG |
| PCDna3.1Split1&2C_R_Flag_NLS | CTTCTTTGGCTTATCGTCATCGTCTTTGTAATCCATGGTGGCGGATCCACTAG |
| PCDna3.1Split1N_F_NLS | GAAGGTCGGTATCCACGGAGTCCCAGCAGCCTGAAAGCTTAAGTTTAAACCGCTGATCAGC |
| PCDna3.1Split2N_F_NLS | GAAGGTCGGTATCCACGGAGTCCCAGCAGCCTGAAAGCTTAAGTTTAAACCGCTG |
| FS_47_Cas9#49535_BamHI_fw | ATCGCGGATCCGCCACCATGGATTAC |
| FS_48_Cas9#49535_HindIII_rv | GCGATAAGCTTTTTCTTTTTCTTAGCTTGACCAGC |
| LOV-muta-PstI-middle2_F | GTGAAGAAATTTTGGGAAGAAACTGTAGGTTTCTACAAGGTCCTG |
| LOV-muta-PstI-middle2_R | CAGTTTCAGGACCTTGTAGAAACCTACAGTTTCTTCCCAAAATTTC |
Plasmids
| Name | Date | Brief Description | Genotype | Plasmid Map | GenBank-File |
|---|---|---|---|---|---|
| pJJDuet30 | https://www.addgene.org/11962/ | ||||
| pSFBAD09 | https://www.addgene.org/11963/ | ||||
| pHYRSF1 | https://www.addgene.org/34549/ | ||||
| pHYRSF1-02 | https://www.addgene.org/24765/ | ||||
| pGBMCS-SortA | https://www.addgene.org/21931/ |
Instruments
| Instrument | Type | Manufacturer |
|---|---|---|
| Table Centrifuge | Microfuge® 18 Centrifuge | Beckman CoulterTM |
| Table Centrifuge | Microfuge® 22R Centrifuge | Beckman CoulterTM |
| Centrifuge | Allegra X-12R | Beckman CoulterTM |
| Shaker | VORTEXGENIE 2 | Scientific Industries, SITM |
| Heating plate (magnet stirrer) | MR Hei-Standard | Heidolph |
| Heatblock | QBD4 | Grant |
| Heatblock (shakeing function) | Thermomixer comfort | Eppendorf |
| PCR-Machine | MyCyclerTM thermo cycler | BioRad |
| PCR-Machine | T100 Therma Cycler | BioRad |
| UV-Chamber | Transluminator | Vilber Lourmat |
| Scale (fine) | PioneerTM PA114C | OHAUS |
| Scale | PioneerTM PA4101C | OHAUS |
| FACS | Cytomics FC 500 MPL | Beckman Coulter |
| Fridge | KTP 1750 Premium | Liebherr |
| Freezer | GP 1366 Premium | Liebherr |
| Hood | Tischabzug | Wesemann® Laboreinrichtung |
| Draw-Off Pump | Vacuhand control | Vacubrand |
| Incubator | HT Multitron Version 2 | INFORS |
| Plate Reader | Infinite M200 | Tecan |
| Computer | Sun Microsystems | |
| UV/VIS Spectrometer | Ultrospec 3300 pro | Amersham Biosciences |
| Photometer | NanoDrop® ND-1000 Spectrophotometer | peQLab Biotechnologie GmbH |
| Photometer | NanoVue | General Electric |
| Ice Machine | MF22 | SCOTSMAN® |
| Gel Electrophoresis Chamber | Mupid®-One | Advance |
| Mini-PROTEAN Tetra Cell | 165-8001 | BioRad |
| Cell Density Meter | Ultrospec10 | Amersham Biosciences |
| PH-Meter | PH-Meter 765 Calimatic | Knick |
| Incubator | Heraeus | Thermo Scientific |
| Lyophilizer | Gefriertrocknungsanlage ALPHA 1-2 LD Plus Best. Nr. 101521 | Christ |
| Ultra Sonification Stick | Sonoplus Gm 2070 (2002) | Bandelin |
| Vacuum Manifold | Qiavac 24 Plus | QIAGEN |
| Gas Cartridge Ventil CV470 | Burner | 13883 |
| NuPAGE(R) Novex 3-8% Tris-Acetate Gel 1 | SDS-Gel | Life Technologies GmbH |
Lab Materials
| Material | Name | Manufacturer |
|---|---|---|
| Bottle 50 ml, 100 ml, 200 ml, 500 ml, 1000 ml | ||
| Beaker 50 ml, 100 ml, 200 ml, 500 ml 1000 ml | ||
| Conical Flask 500 ml, 1000 ml, 100 ml | ||
| Conical Flask 300 ml | ||
| Single Channel Pipette | Pipetman® P2, P20, P200, P1000 | Gilson |
| Multichannel Pipette | ||
| Multistep Pipette | ||
| Pipette | ||
| Scalpels | Bayha | 13569 |
| HISTRAP HP 5 X 1 ML, 5 EA | Th. Geyer GmbH | AM/17524701/000001 |
| neoScrew-Microtubes 1,5 ml | NEOLAB GMBH | 290174576 |
| CM SEPHADEX | Sigma-Aldrich Chemie GmbH | C50120-10G |
| Dialysis Tubing | 1784.1 | Carl Roth GmbH |
| Amicon Ultra 15ml 30K 8pk | UFC903008 | Merck Chemicals GmbH |
| 0.2 ml PCR Tube, Flat Cap, Natural | I1402-8200 | Starlab GmbH |
| KLEENEX KIMWIPES | Kimberly-Clark | 4254 |
| Microcentrifuge Tubes | ||
| Centrifugetubes | ||
| PCR 8-Strips | ||
| Gloves Nitril Gr. L | Starlab powderfree | Starlab |
| Gloves Nitril Gr. M | Starlab powderfree | Starlab |
| Gloves Nitril Gr. S | Starlab powderfree | Starlab |
| Filtertips | ||
| Inoculating Loop | ||
| Disposal Bags | ||
| Flasks | ||
| Plate | ||
| Petri Dishes | P5731-500EA | Sigma-Aldrich Chemie GmbH |
| Petri Dishes | N221.2 | Carl Roth GmbH & Co.KG |
| Petri Dishes | 10558071 | Fischer Scientific |
| TipOne® Pipette Tip | S1110-1700 | Starlab GmbH |
| TipOne® Pipette Tip, 1000µl, Graduated | S1111-2721 | Starlab GmbH |
| NeoBox-81 6er Set, je 1 x Transparent, g | 22916 | |
| NeoLab-Marker for Reaction-Flaks | 19079 | |
| Gene Pulser/MicroPulser Cuvettes, 0.1 cm | 165-2089 | |
| NeoLab-Paper Scissors, 23 cm Long, Even | 23272 | |
| TipOne® Pipette Tip, 200µl | S1110-1700 | Starlab GmbH |
| TipOne® Pipette Tip, 10µl, Graduated, Re | S1111-3700 | Starlab GmbH |
| Pipette, 5 ml | 606180 | Greiner bio-one GmbH |
| Micro Plates, 96 well | 655101 | Greiner bio-one GmbH |
| Ring out of Plumbum with Vinyl Coating, 57 mm In | 310161013 | NEOLAB GmbH |
| Reaction Tube,S.L.1.5 ml,Colorless. | 12682 | Eppendorf, Fisher Scientific GmbH |
| Reaction Tube,S.L.,2 ml, Colorless | 12776 | Eppendorf |
| NeoTape-Writing Tape, 13 mm, Gray | 280126114 | NEOLAB GMBH |
| NeoTape-Writing Tape, 25 mm, Salmon-Colored | 280126229 | NEOLAB GMBH |
| 10 ml Serological Pipette, Filter, Sterile | E4860-1011 | Starlab GmbH |
| Gloves Latex + Alovera L | 14089 | |
| Gloves Latex + Alovera M | 14088 | |
| Gloves Latex + Alovera S | 14087 | |
| CYTOONE 96W F-BTM TC PLT | CC7682-7596 | Starlab Gmbh |
| PH-Stripes | WHAT10362000 | PANPEHA PLUS |
| PCR Plates 96 well | GK96HIGH | Kisker Biotec |
| 100 Run24Barcode | 20110099-100 | GATC Biotech AG |
| Adhesive PCR Film | 82-0558-A | Peqlab GmbH |
| Tube Conical, Polypropylen, 50 ml | 352070 | NEOLAB GMBH |
| Gene Pulser/MicroPulser Cuvettes, 0.1 cm | 165-2089 | Bio-Rad Laboratories GmbH |
| 0.2 ml 8-Tube Strips Without Caps, Nature | TBS-0201 | Bio-Rad Laboratories GmbH |
| Round-Bottom Tubule, Polypropylen, 14 ml | 352059 | NEOLAB GMBH |
| Optical Flat 8-Cap Strips | TCS-0803 | Bio-Rad Laboratories GmbH |
| Inoculation Loop 10 µl, Blue, Sterile | 2900254437 | |
| Corning Serological Pipette 50 ml | 14303 | |
| Weighing Dish 500 ST | 1884.1 | Carl Roth GmbH & Co.KG |
| Filter Paper | Z274836-1PAK | Sigma-Aldrich Chemie GmbH |
| Inoculation Loop 10 µl | 2900254437 | NEOLAB GMBH |
| Immobilon-PSQ Membran, PVDF | ISEQ00010 | Merck Milipore |
Further Recipies and Stocks
Ampicillin Stock Solution
| Stock | Ampicillin 100 mg / ml |
| Amount | 50 ml |
| Storage | -24°C freezer |
| Notes | Use in 1:1000 dilution |
Chloramphenicol Stock Solution
| Stock | Chloramphenicol 30 mg / ml |
| Amount | 10 ml |
| Storage | -24°C freezer |
| Notes | Solved in 100% ethanol. Use in 1:3000 dilution |
E.Coli BAP 1 Glycerol Stock
| Stock | E. coli BAP1 glycerol stock |
| Amount | 2 x 1.3 ml |
| Storage | -80°C freezer |
E.Coli BAP1, Competent
| Stock | E. coli BAP1 |
| Amount | 18 x 100 µl |
| Storage | -80°C freezer |
| Notes | Grows extremely fast. Be careful with miniPreps, at least in cultures with ampicillin it tends to degrade all available ampicillin and then lose the respective plasmid. |
E. coli BAP1-pKD46 Glycerol Stock
| What | E. coli BAP1-pKD46 glycerol stock (Ampr) |
| Amount | 2 x 1.3 ml |
| Storage | -80°C freezer |
| Notes | Grow at 30°C only! Growth at 37°C will lead to loss of pKD46 plasmid. |
E.Coli BAP1-pLF03 Glycerol Stock
| Stock | E. coli BAP1-pLF03 glycerol stock (Ampr) |
| Amount | 1 x 1.3 ml |
| Storage | -80°C freezer |
| Notes | Might have low amount of plasmid-carrying bacteria due to long culturing time (all Amp in medium cleaved) |
E. Coli DH5a-pCP20 Glycerol Stock
| Stock | E. coli DH5a-pCP20 glycerol stock (Ampr, Cmr) |
| Amount | 2 x 1.3 ml |
| Storage | -80°C freezer |
| Notes | Grow at 30°C only! Growth at 37°C will lead to loss of pCP20 plasmid. |
Heat-Shock Competent E. Coli TOP10
| Stock | E. coli TOP10, Heat-shock competent |
| Amount | 200 x 100 µl |
| Storage | -80°C freezer |
| Notes | Verified on 2013-06-07. |
E. Coli TOP10-BBa I746200/pSB1AK3 Glycerol Stock
| Stock | E. coli TOP10-(BBa I746200 in pSB1AK3) (Ampr, Kanr) |
| Amount | 1 x 1.3 ml |
| Storage | -80°C freezer |
E. Coli TOP10-BBa J04450/pSB3C5 Glycerol Stock
| Stock | E. coli TOP10-(BBa_J04450 in pSB3C5) glycerol stock (Cmr) |
| Amount | 1 x 1.3 ml |
| Storage | -80°C freezer |
E. Coli TOP10-pIK1 Glycerol Stock
| Stock | E. coli TOP10-pIK1 glycerol stock (Cmr) |
| Amount | 1 x 1.3 ml |
| Storage | -80°C freezer |
E. Coli TOP10-pKD46 Glycerol Stock
| Stock | E. coli TOP10-pKD46 glycerol stock (Ampr) |
| Amount | 2 x 1.3 ml |
| Storage | -80°C freezer |
| Notes | Grow at 30°C only! Growth at 37°C will lead to loss of pKD46 plasmid. |
E. Coli TOP10-pMM65 Glycerol Stock
| Stock | E. coli TOP10-pMM65 glycerol stock (Kanr) |
| Amount | 1 x 1.3 ml |
| Storage | -80°C freezer |
IPTG Stock Solution
| Stock | IPTG 23.8 mg / ml |
| Amount | 10 ml |
| Storage | -20°C freezer |
| Notes | Use 0.1 to 1 mM |
Kanamycin Stock Solution
| Stock | Kanamycin 50 mg / ml |
| Amount | 10 ml |
| Storage | -24°C freezer |
| Notes | Use in 1:1000 dilution |
M9 Medium
| Name | M9 Minimal Salts 5x, Powder |
| Amount | 1 l |
| Storage | room temperature |
| Notes | close tightly, hygroscopic |
- Add 200 ml of sterile M9 salt solution to 750 ml sterile, distilled H2O (45-50°C)
- Add sterile 20 ml 20% Glucose-solution, 2 ml 1 M MgSO4 and (optionally) 1 M CaCal
Reactivation Medium
for 1L
- 5.0 g Peptone
- 3.0 g Meat extract
- 1000.0 ml Distilled water
