Team:Hong Kong-CUHK/biobricks.html
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<a href="#sclcd">ScLCD - Saccharomyces cerevisiae<br> Liquid Chip Display</a> | <a href="#sclcd">ScLCD - Saccharomyces cerevisiae<br> Liquid Chip Display</a> | ||
</li> | </li> | ||
- | + | ||
</ul> | </ul> | ||
</nav> | </nav> | ||
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<section id="T7expression" class="group"> | <section id="T7expression" class="group"> | ||
<h2>Nitrogen-repressible T7 expression system</h2> | <h2>Nitrogen-repressible T7 expression system</h2> | ||
- | + | <h3>Basic Parts</h3> | |
<div id="subgroup-basic" class="subgroup well"> | <div id="subgroup-basic" class="subgroup well"> | ||
<table class="table table-hover"> | <table class="table table-hover"> | ||
Line 103: | Line 103: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
- | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314000" target="_blank">BBa_K1314000</a></td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314000" target="_blank">BBa_K1314000</a> |
+ | </td> | ||
<td>Ammonia repressible nifH promoter</td> | <td>Ammonia repressible nifH promoter</td> | ||
- | <td><ol> | + | <td> |
- | + | <ol> | |
- | + | <li>in pSB1C3(submitted)</li> | |
- | + | <li>in pSB1A3(stocked)</li> | |
+ | </ol> | ||
+ | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314002" target="_blank">BBa_K1314002</a></td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314002" target="_blank">BBa_K1314002</a> |
+ | </td> | ||
<td>150bp random sequence (sequence for homology)</td> | <td>150bp random sequence (sequence for homology)</td> | ||
- | <td><ol><li>in pSB1C3(submitted)</li></ol></td> | + | <td> |
+ | <ol> | ||
+ | <li>in pSB1C3(submitted)</li> | ||
+ | </ol> | ||
+ | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314003" target="_blank">BBa_K1314003</a></td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314003" target="_blank">BBa_K1314003</a> |
+ | </td> | ||
<td>part of mRFP(sequence for homology)</td> | <td>part of mRFP(sequence for homology)</td> | ||
<td>Not submitted</td> | <td>Not submitted</td> | ||
</tr> | </tr> | ||
- | + | <tr> | |
- | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314012" target="_blank">BBa_K1314012</a></td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314012" target="_blank">BBa_K1314012</a> |
+ | </td> | ||
<td>nif K homologous sequence</td> | <td>nif K homologous sequence</td> | ||
<td>Not submitted</td> | <td>Not submitted</td> | ||
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<tbody> | <tbody> | ||
<tr> | <tr> | ||
- | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314001" target="_blank">BBa_K1314001</a></td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314001" target="_blank">BBa_K1314001</a> |
+ | </td> | ||
<td>nifH promoter with mRFP reporter</td> | <td>nifH promoter with mRFP reporter</td> | ||
- | <td><ol> | + | <td> |
- | + | <ol> | |
- | + | <li>in pSB1C3(submitted)</li> | |
- | + | <li>in pSB1A3(stocked)</li> | |
+ | </ol> | ||
+ | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314004" target="_blank">BBa_K1314004</a></td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314004" target="_blank">BBa_K1314004</a> |
+ | </td> | ||
<td>Ptet mRFP with random sequence</td> | <td>Ptet mRFP with random sequence</td> | ||
- | <td><ol><li>in pSB1C3(submitted)</li></ol></td> | + | <td> |
+ | <ol> | ||
+ | <li>in pSB1C3(submitted)</li> | ||
+ | </ol> | ||
+ | </td> | ||
</tr> | </tr> | ||
- | + | <tr> | |
- | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314005" target="_blank">BBa_K1314005</a></td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314005" target="_blank">BBa_K1314005</a> |
+ | </td> | ||
<td>Ammonia- repressible T7 RNA polymerase generator(strong)</td> | <td>Ammonia- repressible T7 RNA polymerase generator(strong)</td> | ||
- | <td><ol><li>in pSB1C3(submitted)</li></ol></td> | + | <td> |
+ | <ol> | ||
+ | <li>in pSB1C3(submitted)</li> | ||
+ | </ol> | ||
+ | </td> | ||
</tr> | </tr> | ||
- | + | <tr> | |
- | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314006" target="_blank">BBa_K1314006</a></td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314006" target="_blank">BBa_K1314006</a> |
+ | </td> | ||
<td>Ammonia- repressible T7 RNA polymerase generator(weak)</td> | <td>Ammonia- repressible T7 RNA polymerase generator(weak)</td> | ||
- | <td><ol><li>in pSB1C3(submitted)</li></ol></td> | + | <td> |
+ | <ol> | ||
+ | <li>in pSB1C3(submitted)</li> | ||
+ | </ol> | ||
+ | </td> | ||
</tr> | </tr> | ||
- | + | <tr> | |
- | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314007" target="_blank">BBa_K1314007</a></td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314007" target="_blank">BBa_K1314007</a> |
+ | </td> | ||
<td>Ammonia- repressible T7 RNA polymerase generator(strong) with ptet mRFP reporter</td> | <td>Ammonia- repressible T7 RNA polymerase generator(strong) with ptet mRFP reporter</td> | ||
- | <td><ol><li>in pSB1C3(submitted)</li></ol></td> | + | <td> |
+ | <ol> | ||
+ | <li>in pSB1C3(submitted)</li> | ||
+ | </ol> | ||
+ | </td> | ||
</tr> | </tr> | ||
- | + | <tr> | |
- | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314008" target="_blank">BBa_K1314008</a></td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314008" target="_blank">BBa_K1314008</a> |
+ | </td> | ||
<td>Ammonia- repressible T7 RNA polymerase generator(weak) with ptet mRFP reporter</td> | <td>Ammonia- repressible T7 RNA polymerase generator(weak) with ptet mRFP reporter</td> | ||
- | <td><ol><li>in pSB1C3(submitted)</li></ol></td> | + | <td> |
+ | <ol> | ||
+ | <li>in pSB1C3(submitted)</li> | ||
+ | </ol> | ||
+ | </td> | ||
</tr> | </tr> | ||
- | + | <tr> | |
- | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314009" target="_blank">BBa_K1314009</a></td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314009" target="_blank">BBa_K1314009</a> |
+ | </td> | ||
<td>Amilcp reporter with part of rfp</td> | <td>Amilcp reporter with part of rfp</td> | ||
- | <td><ol><li>in pSB1C3(submitted)</li></ol></td> | + | <td> |
+ | <ol> | ||
+ | <li>in pSB1C3(submitted)</li> | ||
+ | </ol> | ||
+ | </td> | ||
</tr> | </tr> | ||
- | + | <tr> | |
- | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314010" target="_blank">BBa_K1314010</a></td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314010" target="_blank">BBa_K1314010</a> |
+ | </td> | ||
<td>T7 promoter with random sequence</td> | <td>T7 promoter with random sequence</td> | ||
- | <td><ol> | + | <td> |
- | + | <ol> | |
- | + | <li>in pSB1C3(submitted)</li> | |
- | + | <li>in pSB1AK8(stocked)</li> | |
+ | </ol> | ||
+ | </td> | ||
</tr> | </tr> | ||
- | + | <tr> | |
- | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314011" target="_blank">BBa_K1314011</a></td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314011" target="_blank">BBa_K1314011</a> |
+ | </td> | ||
<td>T7 promoter amilcp reporter with homologous sequence</td> | <td>T7 promoter amilcp reporter with homologous sequence</td> | ||
- | <td><ol><li>in pSB1C3(submitted)</li></ol></td> | + | <td> |
+ | <ol> | ||
+ | <li>in pSB1C3(submitted)</li> | ||
+ | </ol> | ||
+ | </td> | ||
</tr> | </tr> | ||
- | + | <tr> | |
- | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314013" target="_blank">BBa_K1314013</a></td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314013" target="_blank">BBa_K1314013</a> |
+ | </td> | ||
<td>Ammonia- repressible T7 RNA polymerase generator(strong) with ptet mRFP reporter (site-specific integration version)</td> | <td>Ammonia- repressible T7 RNA polymerase generator(strong) with ptet mRFP reporter (site-specific integration version)</td> | ||
- | <td><ol> | + | <td> |
- | + | <ol> | |
- | + | <li>in pSB1C3(submitted)</li> | |
- | + | <li>in pSB1A3(stocked)</li> | |
+ | </ol> | ||
+ | </td> | ||
</tr> | </tr> | ||
- | + | <tr> | |
- | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314014" target="_blank">BBa_K1314014</a></td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314014" target="_blank">BBa_K1314014</a> |
+ | </td> | ||
<td>Ammonia- repressible T7 RNA polymerase generator(weak) with ptet mRFP reporter (site-specific integration version)</td> | <td>Ammonia- repressible T7 RNA polymerase generator(weak) with ptet mRFP reporter (site-specific integration version)</td> | ||
- | <td><ol> | + | <td> |
- | + | <ol> | |
- | + | <li>in pSB1C3(submitted)</li> | |
- | + | <li>in pSB1A3(stocked)</li> | |
+ | </ol> | ||
+ | </td> | ||
</tr> | </tr> | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
</div> | </div> | ||
- | + | <h3>Plasmid Backbone</h3> | |
<div id="subgroup-basic" class="subgroup well"> | <div id="subgroup-basic" class="subgroup well"> | ||
<table class="table table-hover"> | <table class="table table-hover"> | ||
Line 221: | Line 278: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
- | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314000" target="_blank">BBa_K1314000</a></td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314000" target="_blank">BBa_K1314000</a> |
+ | </td> | ||
<td>Homology integration toolkit</td> | <td>Homology integration toolkit</td> | ||
- | <td><ol> | + | <td> |
- | + | <ol> | |
- | + | <li>With Kanamycin (submitted)</li> | |
+ | </ol> | ||
+ | </td> | ||
</tr> | </tr> | ||
</tbody> | </tbody> | ||
Line 234: | Line 294: | ||
<h2>ScLCD- Saccharomyces cerevisiae Liquid Chip Display</h2> | <h2>ScLCD- Saccharomyces cerevisiae Liquid Chip Display</h2> | ||
<div class="subgroup well"> | <div class="subgroup well"> | ||
- | + | <table class="table table-hover"> | |
<thead> | <thead> | ||
<tr> | <tr> | ||
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<tbody> | <tbody> | ||
<tr> | <tr> | ||
- | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314017" target="_blank">BBa_K1314017</a></td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314017" target="_blank">BBa_K1314017</a> |
+ | </td> | ||
<td>Mid1 (S. cerevisae Codon Opt)</td> | <td>Mid1 (S. cerevisae Codon Opt)</td> | ||
- | <td><ol> | + | <td> |
- | + | <ol> | |
- | + | <li>in pSB1C3(submitted)</li> | |
+ | </ol> | ||
+ | </td> | ||
</tr> | </tr> | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
- | + | </div> | |
</section> | </section> | ||
</div> | </div> | ||
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target: '.bs-docs-sidebar', | target: '.bs-docs-sidebar', | ||
offset: 100 | offset: 100 | ||
+ | }); | ||
+ | $(window).scroll(function(e) { | ||
+ | var scrollTop = $(window).scrollTop(); | ||
+ | if (scrollTop > 250) { | ||
+ | $('#sidebar').addClass('fixed').offset({ | ||
+ | top: scrollTop | ||
+ | }); | ||
+ | } else { | ||
+ | $('#sidebar').removeClass('fixed'); | ||
+ | } | ||
}); | }); | ||
}); | }); | ||
</script> | </script> |
Latest revision as of 00:19, 18 October 2014
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<section id="T7expression" class="group">
Contents |
Nitrogen-repressible T7 expression system
Basic Parts
Part | Description | Copies |
---|---|---|
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314000" target="_blank">BBa_K1314000</a> | Ammonia repressible nifH promoter |
|
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314002" target="_blank">BBa_K1314002</a> | 150bp random sequence (sequence for homology) |
|
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314003" target="_blank">BBa_K1314003</a> | part of mRFP(sequence for homology) | Not submitted |
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314012" target="_blank">BBa_K1314012</a> | nif K homologous sequence | Not submitted |
Composite Parts
Part | Description | Copies |
---|---|---|
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314001" target="_blank">BBa_K1314001</a> | nifH promoter with mRFP reporter |
|
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314004" target="_blank">BBa_K1314004</a> | Ptet mRFP with random sequence |
|
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314005" target="_blank">BBa_K1314005</a> | Ammonia- repressible T7 RNA polymerase generator(strong) |
|
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314006" target="_blank">BBa_K1314006</a> | Ammonia- repressible T7 RNA polymerase generator(weak) |
|
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314007" target="_blank">BBa_K1314007</a> | Ammonia- repressible T7 RNA polymerase generator(strong) with ptet mRFP reporter |
|
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314008" target="_blank">BBa_K1314008</a> | Ammonia- repressible T7 RNA polymerase generator(weak) with ptet mRFP reporter |
|
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314009" target="_blank">BBa_K1314009</a> | Amilcp reporter with part of rfp |
|
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314010" target="_blank">BBa_K1314010</a> | T7 promoter with random sequence |
|
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314011" target="_blank">BBa_K1314011</a> | T7 promoter amilcp reporter with homologous sequence |
|
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314013" target="_blank">BBa_K1314013</a> | Ammonia- repressible T7 RNA polymerase generator(strong) with ptet mRFP reporter (site-specific integration version) |
|
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314014" target="_blank">BBa_K1314014</a> | Ammonia- repressible T7 RNA polymerase generator(weak) with ptet mRFP reporter (site-specific integration version) |
|
Plasmid Backbone
Part | Description | Copies |
---|---|---|
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314000" target="_blank">BBa_K1314000</a> | Homology integration toolkit |
|
</section> <section id="sclcd" class="group">
ScLCD- Saccharomyces cerevisiae Liquid Chip Display
Part | Description | Copies |
---|---|---|
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1314017" target="_blank">BBa_K1314017</a> | Mid1 (S. cerevisae Codon Opt) |
|
</section>
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