Team:Sumbawagen

From 2014.igem.org

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                   <a href="#" class="dropdown-toggle" data-toggle="dropdown">Overviews <b class="caret"></b></a>
                   <a href="#" class="dropdown-toggle" data-toggle="dropdown">Overviews <b class="caret"></b></a>
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                       <li><a href="https://2014.igem.org/Team:Sumbawagen/overviews/Project"> Project </a></li>
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                       <li><a href="https://2014.igem.org/Team:Sumbawagen/overviews/Econey_Project"> Econey Project </a></li>
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                      <!--<li><a href="#">Sub Menu 2 </a></li>-->
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                     </ul>
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                      <li><a href="https://2014.igem.org/Team:Sumbawagen/Modeling">Modelling </a></li>
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                      <li><a href="https://2014.igem.org/Team:Sumbawagen/overviews/Our_Software">Our Software</a></li>
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                     <li><a href="https://2014.igem.org/Team:Sumbawagen/humanpractice_overview">Human Practice </a></li>
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                   <a href="https://2014.igem.org/Team:Sumbawagen/Team" class="dropdown-toggle" data-toggle="dropdown">Project <b class="caret"></b></a>
                   <a href="https://2014.igem.org/Team:Sumbawagen/Team" class="dropdown-toggle" data-toggle="dropdown">Project <b class="caret"></b></a>
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                    <li class="nav-header">Lab</li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/project/econey">Econey</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/project/result">Result</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/project/software">Software</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/parts">Parts</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Safety">Safety</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Attributions">Attribution</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/future_direction">Future Direction</a></li>                             
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<li><a href="https://2014.igem.org/Team:Sumbawagen/project/lab">Biotechnology</a></li>
 
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                    <li class="nav-header">Our Software </li>
 
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                    <li class="nav-header">Software &amp; Hardware</li>
 
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                  <li><a href="#">Glucose Measurement</a></li>
 
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                  <li><a href="#">Android Application</a></li>
 
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                    <li class="nav-header">Attributions </li>
 
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                    <li><a href="https://2014.igem.org/Team:Sumbawagen/Attributions">Attributions </a></li>
 
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                   <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook <b class="caret"></b></a>
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                     <li><a href="#">Our Biobricks</a></li>
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                    <li class="nav-header">Daily Notes</li>
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                    <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook">Policy & Practices</a></li>
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                    <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook2">Lab</a></li>
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                     <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook/Protocol">Protocol</a></li>
                     <!--<li><a href="#">Improvements</a></li>-->
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                 <li><a href="https://2014.igem.org/Team:Sumbawagen/Safety">Safety</a></li>
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                   <ul class="dropdown-menu">
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                     <li><a href="https://2014.igem.org/Team:Sumbawagen/Team">Meet the Team</a></li>
                     <li><a href="https://2014.igem.org/Team:Sumbawagen/Team">Meet the Team</a></li>
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                    <li><a href="https://igem.org/Team.cgi">Team Information</a></li>
 
                     <li><a href="https://2014.igem.org/Team:Sumbawagen/Team/Gallery">Gallery</a></li>
                     <li><a href="https://2014.igem.org/Team:Sumbawagen/Team/Gallery">Gallery</a></li>
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                    <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook">Notebook</a></li>
 
                     <li><a href="https://2014.igem.org/Team:Sumbawagen/Team/Contact">Contact</a></li>
                     <li><a href="https://2014.igem.org/Team:Sumbawagen/Team/Contact">Contact</a></li>
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                   <a href="#" class="dropdown-toggle" data-toggle="dropdown">Policy & Practices<!--<sup>*new</sup>--><b class="caret"></b></a>
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Sub Menu 41
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                     <li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/Policy">
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<strong>Policy & Practices</strong>
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Sub Menu 42
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/survey">Survey</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/Biotech">Biotech Camp</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/meet_the">Meet the farmers</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/meet">Meet US Ambassador</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/econey">@america</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/moyo">Moyo Festival</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/virtual">Virtual Outreach</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/Support">Responses and supports</a></li>
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  <sup>*New</sup></div>
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                  <a href="#" class="dropdown-toggle" data-toggle="dropdown">Interlab Study <b class="caret"></b></a>
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                  <ul class="dropdown-menu">
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                    <li><a href="https://2014.igem.org/Team:Sumbawagen/interlabstudy/results">Results</a></li>
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        <h2 style="margin-top:-10px;"> Project Description </h2>
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        BBa_J04450 has a lac promoter and mRFP gene, thus E. coli harboring this plasmid will give red color when the bacteria were grown with lactose or IPTG. Oppositely, the expression of mRFP will be decreased when glucose was present in medium through a mechanism called catabolite repression. We use this phenomenon to measure the concentration of glucose in honey by calibrating the color of E. coli medium using android-based mobile phone. Creation of novel circuit which consists of constitutive promoter followed by either Adenylate Cyclase or IIAGlc genes, which placed downward lac-mRFP circuit, may affect the sensitivity of catabolite repression. We expect an engineered E. coli, which shows red color expression in different range of glucose concentration. By changing mRFP gene with amilCP, a blue fluorescent protein, we may obtain E. coli expressing either red or blue color when different honey added to the medium due to different glucose concentration.<br><br></p>
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        <a href="https://2014.igem.org/Team:Sumbawagen">
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                          <p><b style="font-size:16px">Home</b><br><br> </p>
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              <span class="caption scale-caption" style="text-align:justify">
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                          <p><b style="font-size:16px;">Policy & Practices </b><br><br> </p>
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                          <p><b style="font-size:16px;">Team</b><br><br>  </p>
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                          <p><b style="font-size:16px;">ECONEY Project </b><br><br></p>
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          <center><h2><b><strong>Acknowledgment<strong></b> </h2></center>
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          <center><h2><b><strong>Sponsors</strong></b> </h2></center>
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Latest revision as of 07:50, 19 February 2015

Team:Dundee/Team - 2013.igem.org

 

Team:Sumbawagen/Team

From 2014.igem.org

iGEM Sumbawagen 2014 · Econey

Sumbawagen

Project Description

BBa_J04450 has a lac promoter and mRFP gene, thus E. coli harboring this plasmid will give red color when the bacteria were grown with lactose or IPTG. Oppositely, the expression of mRFP will be decreased when glucose was present in medium through a mechanism called catabolite repression. We use this phenomenon to measure the concentration of glucose in honey by calibrating the color of E. coli medium using android-based mobile phone. Creation of novel circuit which consists of constitutive promoter followed by either Adenylate Cyclase or IIAGlc genes, which placed downward lac-mRFP circuit, may affect the sensitivity of catabolite repression. We expect an engineered E. coli, which shows red color expression in different range of glucose concentration. By changing mRFP gene with amilCP, a blue fluorescent protein, we may obtain E. coli expressing either red or blue color when different honey added to the medium due to different glucose concentration.


Acknowledgment


Sponsors



Retrieved from "https://2013.igem.org/Team:Dundee"

Retrieved from "https://2014.igem.org/Team:Sumbawagen"

Retrieved from "http://2014.igem.org/Team:Sumbawagen"