Team:NEFU China/Recycling

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           <p>In order  to verify the synthesis of the CdS nanocrystal in <em>E.coli</em> (<em>Rosetta  plysS</em>), the bacteria was harvested by centrifuged  in 3000*g for 3 min then suspended in resin and hardened at 60℃ for 16 hours.</p>
           <p>In order  to verify the synthesis of the CdS nanocrystal in <em>E.coli</em> (<em>Rosetta  plysS</em>), the bacteria was harvested by centrifuged  in 3000*g for 3 min then suspended in resin and hardened at 60℃ for 16 hours.</p>
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           <p>Then the hard pellets were cut into 60nm thin slices and the slices were floated on water and deposited on a carbon-coated copper TEM grid. Microscopy was performed with a JEM-1400 microscope at 120-keV electron energy.</p>
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           <p>Then the hard pellets were cut into 60nm thin slices and the slices were floated on water and deposited on a carbon-coated copper TEM grid. Microscopy was performed with a JEM-1400 microscope at 80-keV electron energy.</p>
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           <h2 class="subtitle">Reference.</h2>
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           <li>Ivan Sondi,Branka Salopek-Sondi.Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. Journal of Colloid and Interface Science 275 (2004) 177–182</li>
           <li>Ivan Sondi,Branka Salopek-Sondi.Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. Journal of Colloid and Interface Science 275 (2004) 177–182</li>
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Team: NEFU_China<br>
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Email: yichengzhao@live.cn<br>
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Northeast Forestry University, Harbin, China
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Latest revision as of 23:28, 17 October 2014

Recycling

TEM Protocol.

In order to verify the synthesis of the CdS nanocrystal in E.coli (Rosetta plysS), the bacteria was harvested by centrifuged in 3000*g for 3 min then suspended in resin and hardened at 60℃ for 16 hours.

Then the hard pellets were cut into 60nm thin slices and the slices were floated on water and deposited on a carbon-coated copper TEM grid. Microscopy was performed with a JEM-1400 microscope at 80-keV electron energy.

Reference.

  1. Mareike Hartmann,Marina Berditsch,Jacques Hawecker,Mohammad Fotouhi Ardakani,Dagmar Gerthsen,Anne S. Ulrich1.Damage of the Bacterial Cell Envelope by Antimicrobial Peptides Gramicidin S and PGLa as Revealed by Transmission and Scanning Electron Microscopy Antimicrob Agents Chemother. 2010; 54(8): 3132–3142.
  2. Congcong Mi,Yanyan Wang,Jingpu Zhang,Huaiqing Huang,Linru Xu,Shuo Wang,Xuexun Fang,Jin Fang,Chuanbin Mao,Shukun Xu.Biosynthesis and characterization of CdS quantum dots in genetically engineered Escherichia coli. Journal of biotechnology 153(2011) 125-132
  3. Ivan Sondi,Branka Salopek-Sondi.Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. Journal of Colloid and Interface Science 275 (2004) 177–182
Team: NEFU_China
Email: yichengzhao@live.cn
Northeast Forestry University, Harbin, China

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