Team:Peking

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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Peking&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<a href="https://2014.igem.org/Team:Peking"style="color:#000000">Home </a> </td>
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<a href="https://2014.igem.org/Team:Peking/Team"style="color:#000000"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=Peking"style="color:#000000"> Official Team Profile </a></td>
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<a href="https://2014.igem.org/Team:Peking/Project"style="color:#000000"> Project</a></td>
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<a href="https://2014.igem.org/Team:Peking/Parts"style="color:#000000"> Parts</a></td>
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<a href="https://2014.igem.org/Team:Peking/Notebook"style="color:#000000"> Notebook</a></td>
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<a href="https://2014.igem.org/Team:Peking/Safety"style=" color:#000000"> Safety </a></td>
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<a href="https://2014.igem.org/Team:Peking/Attributions"style="color:#000000"> Attributions </a></td>
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<tr><td colspan="3"> <h3> Requirements </h3></td></tr>
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<p> Please be sure to keep these links, your audience will want to find your: </p>
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<!-- Links to other team pages -->
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<ul>
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<li><a href="https://2014.igem.org/Team:Peking">Home</a> </li>
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<li><a href="https://2014.igem.org/Team:Peking/Team">Team</a> </li>
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<li><a href="https://igem.org/Team.cgi?year=2013&team_name=Peking">Official Team Profile</a> </li>
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<li><a href="https://2014.igem.org/Team:Peking/Project">Project</a> </li>
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<li><a href="https://2014.igem.org/Team:Peking/Parts">Parts</a> </li>
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<li><a href="https://2014.igem.org/Team:Peking/Modeling">Modeling</a> </li>
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<li><a href="https://2014.igem.org/Team:Peking/Notebook">Notebook</a> </li>
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<li><a href="https://2014.igem.org/Team:Peking/Safety">Safety</a> </li>
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<li><a href="https://2014.igem.org/Team:Peking/Attributions">Attributions</a> </li>
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<p>There are a few wiki requirements teams must follow:</p>
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<li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload">  2014.igem.org server</a>. </li>
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<li>All pages must be created under the team’s name space.</li>
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<li>As part of your documentation, keep the links from the menu to the left. </li>
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<li>Do not use flash in wiki code. </li>
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<li>The <a href="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"> iGEM logo </a> should be placed on the upper part of every page and should link to <a href="https://2014.igem.org/Main_Page">2014.igem.org</a>.</li>
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<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
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<p>We are currently working on providing teams with some easy to use design templates.
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<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
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<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
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    <script type="text/javascript" src="https://2014.igem.org/Team:Peking/js/sliderman.1.3.8.js?action=raw&ctype=text/javascript"></script>
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<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
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    <link rel="stylesheet" type="text/css" href="https://2014.igem.org/Team:Peking/css/sliderman.css?action=raw&ctype=text/css" />
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<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
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<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
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<li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
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<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
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<img src="https://static.igem.org/mediawiki/2014/7/7f/Peking2014wcg_title.png" alt=""/>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<div id="mid1">
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      <p class="habstract" style="text-align:center">Abstract</p>
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      <div id="pabstract"><p>Widespread algal blooms cause extensive damage in ecosystems. Compared to physical or chemical methods, biological treatment for algal bloom is less expensive and more environmentally friendly. Peking iGEM is dedicated to constructing engineered microorganisms for the elimination of cyanobacteria and recovery of ecosystems.</p>
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<p>An antimicrobial lysozyme was introduced to kill cyanobacteria since lysozyme could disrupt the outer membrane of cyanobacteria. In addition, we equipped our transgenic cells with features that allow for buoyancy and attachment, making our project more efficient. During this process, an enzyme is also secreted to degrade a deleterious product of cyanobacteria. After eradicating the cyanobacteria, our engineered bacteria will commit suicide, and the ecosystem is finally restored. </p>
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<p>This project is an innovative treatment for water bloom, and has potential applications in the field of ecosystem remediation.
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</p></div>
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        <img src="https://static.igem.org/mediawiki/2014/0/07/Peking2014wcg_slideshow1.png"  alt="Demo2 first" />
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        <div class="SliderName_2Description">Peking iGEM: <strong>Lab</strong></div>
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        <img src="https://static.igem.org/mediawiki/2014/b/bd/Peking2014ylq_algae_photo3.jpg"  alt="Demo2 first" />
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        <div class="SliderName_2Description">Peking iGEM: <strong>The Killing effects</strong></div>
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        <img src="https://static.igem.org/mediawiki/2014/6/66/Peking2014wcg_slideshow11.png"  />
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        <div class="SliderName_2Description">Peking iGEM: <strong>Peking iGEM Club</strong></div>
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        <img src="https://static.igem.org/mediawiki/2014/5/5d/Peking2014wcg_slideshow5.png"  />
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        <div class="SliderName_2Description">Peking iGEM: <strong>Fluorescence Picture</strong></div>
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effectsDemo2 = 'rain,stairs,fade';
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var demoSlider_2 = Sliderman.slider({container: 'SliderName_2', width: w, height: h, effects: effectsDemo2,
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display: {
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autoplay: 3000,
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loading: {background: '#000000', opacity: 0.5, image: 'img/loading.gif'},
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      <p class="subgroupl"> <strong><a href="https://2014.igem.org/Team:Peking/Killing" style="text-decoration: none;">Killing:</a></strong><br />
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        Killing the cyanobacteria is the directly efficient way to reduce an algal bloom. Lysozyme was introduced to annihilate cyanobacteria. The lysozyme could deconstruct the outer membrane of cyanobacteria and further lyse the cyanobacteria. Lysozyme immune system was implemented to protect our genetically engineered <i>E. coli</i>. Our goal is secreting lysozyme to kill cyanobacteria without harming our <i>E. coli</i>.  </p>
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    <div class="subgroupl">
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      <p > <strong><a href="https://2014.igem.org/Team:Peking/KillingImprovements" style="text-decoration: none;" >Killing Improvements:</a></strong><br />
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A lectin of the cyanobacteria was expressed on the surface of our <i>E. coli</i> cells so that <i>E. coli</i> can bind to the cyanobacteria. Our experimental results show that <i>E. coli</i> cells do bind to the cyanobacteria, indicating that the killing efficiency of our project is greatly enhanced according our computational models. We also equipped our <i>E. coli</i> with gas vesicles so that our project can be feasible in the real ecosystems.
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  <div id="mid2center" > <a href="https://2014.igem.org/Team:Peking/Overview"><img src="https://static.igem.org/mediawiki/2014/9/94/Peking2014wcg_homeprocess.png"  alt=""/> </a></div>
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      <p> <strong>This year, Peking iGEM aimed at treating the problem of algal bloom with synthetic biological tools. Genetically engineered <i>E. coli</i> was implemented to both reduce the algal biomass and degrade the toxin. </strong> </p>
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    </div>
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    <div class="subgroupr">
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      <p> <strong><a href="https://2014.igem.org/Team:Peking/Degradation" style="text-decoration: none;">Degradation :</a></strong><br />
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<i>Microcystis aeruginosa</i> is the most common cyanobacteria during an algal bloom, which can secrete a deadly toxin microcystin. To decrease the negative effects of the toxin on water ecosystems, we introduced an engineered bacteria that can secrete a microcystinase-MlrA protein to hydrolyze the toxin.
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      <p> <strong><a href="https://2014.igem.org/Team:Peking/Suicide" style="text-decoration: none;">Suicide :</a></strong><br />
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After killing cyanobacteria and degrading toxin, the remaining bacteria should be eliminated or they would potentially cause negative effects to the environment. We constructed the suicide system using lysing system from phage, since <i>E. coli</i> should be controlled to commit suicide after accomplishing their mission.
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      <p> <strong><a style="text-decoration: none;" href="https://2014.igem.org/Team:Peking/BindingEvaluation">Modeling :</a></strong><br />
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Our modeling focused on supporting the whole project. We used Monte Carlo simulation to analyze the enhancement of binding of <i>E. coli</i> to cyanobacteria to the efficiency of killing. Another model about cellular burden was built to find optimal expressing efficiency. Furthermore, a macroscopic simulation that predicts the real situation would provide insight for the whole project.
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  <div id="mid3center"> <img src="https://static.igem.org/mediawiki/2014/6/60/Peking2014wcg_process2.png" href="https://2014.igem.org/Team:Peking/Overview" alt=""/> </div>
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      <p> <strong><a style="text-decoration: none;" href="https://2014.igem.org/Team:Peking/TeamCom">Human Practice :</a></strong><br />
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Investigation lies much beyond lab bench. Great insight would be discovered by <i>in field</i> study. We visited Taihu Lake, one of the biggest lake in China suffering algal bloom. We interviewed the researchers and acquired knowledge about cyanobacteria in lake ecosystem. Besides, we were dedicated to communicating with other teams. We also established iGEM Club in Peking University to popularize iGEM competition.
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and what you plan to do.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
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<li>Have lots of fun! </li>
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Latest revision as of 03:47, 18 October 2014

Abstract

Widespread algal blooms cause extensive damage in ecosystems. Compared to physical or chemical methods, biological treatment for algal bloom is less expensive and more environmentally friendly. Peking iGEM is dedicated to constructing engineered microorganisms for the elimination of cyanobacteria and recovery of ecosystems.

An antimicrobial lysozyme was introduced to kill cyanobacteria since lysozyme could disrupt the outer membrane of cyanobacteria. In addition, we equipped our transgenic cells with features that allow for buoyancy and attachment, making our project more efficient. During this process, an enzyme is also secreted to degrade a deleterious product of cyanobacteria. After eradicating the cyanobacteria, our engineered bacteria will commit suicide, and the ecosystem is finally restored.

This project is an innovative treatment for water bloom, and has potential applications in the field of ecosystem remediation.

Demo2 first
Peking iGEM: Lab
Demo2 first
Peking iGEM: The Killing effects
Peking iGEM: Peking iGEM Club
Peking iGEM: Fluorescence Picture

Killing:
Killing the cyanobacteria is the directly efficient way to reduce an algal bloom. Lysozyme was introduced to annihilate cyanobacteria. The lysozyme could deconstruct the outer membrane of cyanobacteria and further lyse the cyanobacteria. Lysozyme immune system was implemented to protect our genetically engineered E. coli. Our goal is secreting lysozyme to kill cyanobacteria without harming our E. coli.

Killing Improvements:
A lectin of the cyanobacteria was expressed on the surface of our E. coli cells so that E. coli can bind to the cyanobacteria. Our experimental results show that E. coli cells do bind to the cyanobacteria, indicating that the killing efficiency of our project is greatly enhanced according our computational models. We also equipped our E. coli with gas vesicles so that our project can be feasible in the real ecosystems.

This year, Peking iGEM aimed at treating the problem of algal bloom with synthetic biological tools. Genetically engineered E. coli was implemented to both reduce the algal biomass and degrade the toxin.

Degradation :
Microcystis aeruginosa is the most common cyanobacteria during an algal bloom, which can secrete a deadly toxin microcystin. To decrease the negative effects of the toxin on water ecosystems, we introduced an engineered bacteria that can secrete a microcystinase-MlrA protein to hydrolyze the toxin.

Suicide :
After killing cyanobacteria and degrading toxin, the remaining bacteria should be eliminated or they would potentially cause negative effects to the environment. We constructed the suicide system using lysing system from phage, since E. coli should be controlled to commit suicide after accomplishing their mission.

Modeling :
Our modeling focused on supporting the whole project. We used Monte Carlo simulation to analyze the enhancement of binding of E. coli to cyanobacteria to the efficiency of killing. Another model about cellular burden was built to find optimal expressing efficiency. Furthermore, a macroscopic simulation that predicts the real situation would provide insight for the whole project.

Human Practice :
Investigation lies much beyond lab bench. Great insight would be discovered by in field study. We visited Taihu Lake, one of the biggest lake in China suffering algal bloom. We interviewed the researchers and acquired knowledge about cyanobacteria in lake ecosystem. Besides, we were dedicated to communicating with other teams. We also established iGEM Club in Peking University to popularize iGEM competition.

Retrieved from "http://2014.igem.org/Team:Peking"