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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Macquarie_Australia&action=edit"style="color:#FFFFFF"> Click here to edit this page!</a> </p>
<p>There are a few wiki requirements teams must follow:</p>
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<li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload"> 2014.igem.org server</a>. </li>
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<li>All pages must be created under the team’s name space.</li>
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<li>As part of your documentation, keep the links from the menu to the left. </li>
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<li>Do not use flash in wiki code. </li>
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<li>The <a href="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"> iGEM logo </a> should be placed on the upper part of every page and should link to <a href="https://2014.igem.org/Main_Page">2014.igem.org</a>.</li>
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<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
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<h1>Our project</h1>
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<tr><td colspan="3" > <h3> Tips </h3></td></tr>
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<h2 style="text-align: center;">Watch this cool video below!</h2>
<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a> lists. </p>
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<h3>Overview</h3>
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<p>This project has demonstrated functionality of our designed operons that represent the first step of the chlorophyll a biosynthesis. This was performed through the initial assembly of three operons containing the essential biosynthetic genes that were confirmed through gel electrophoresis and DNA sequencing (Fig. 1). The functionality of the first operon (Mg-chelatase) was demonstrated through the spectral analysis of its enzymatic product, Mg-Protophoryin IX (which can be seen on the <a href="https://2014.igem.org/Team:Macquarie_Australia/Project/Results">Results</a> page). </p><br/>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
<p><b>Figure 1.</b> Flow chart of the chlorophyll a synthesis pathway. Operons containing the essential genes from <i>Chlamydomonas reinhardtii</i> are represented for their respective steps within the pathway. The spectral change of the compounds are represented in their respective colours of each step.</p>
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<p>We have also modelled this step of the biosynthetic pathway (which can be seen on the <a href="https://2014.igem.org/Team:Macquarie_Australia/Project/Model">Modelling</a> page). The project was successful in building the foundations for future teams to complete the synthesis of photosystem II in <i>E. coli.</i> This provides a significant leap into the development of a hydrogen-generating bacterial system and a renewable biological energy source. Our policy and practice initiatives were also successful for increasing public awareness of the global energy crisis and the potential synthetic biology has to offer in providing a solution (which can be seen on the <a href="https://2014.igem.org/Team:Macquarie_Australia/Outreach">Policy & Practice</a> page). </p>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and what you plan to do.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
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<h2 style="text-align: center;">A huge thank you to our Gold sponsors!</h2>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
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<h2 style="text-align: center;">Also many thanks to our Bronze sponsors!</h2>
This project has demonstrated functionality of our designed operons that represent the first step of the chlorophyll a biosynthesis. This was performed through the initial assembly of three operons containing the essential biosynthetic genes that were confirmed through gel electrophoresis and DNA sequencing (Fig. 1). The functionality of the first operon (Mg-chelatase) was demonstrated through the spectral analysis of its enzymatic product, Mg-Protophoryin IX (which can be seen on the Results page).
Figure 1. Flow chart of the chlorophyll a synthesis pathway. Operons containing the essential genes from Chlamydomonas reinhardtii are represented for their respective steps within the pathway. The spectral change of the compounds are represented in their respective colours of each step.
We have also modelled this step of the biosynthetic pathway (which can be seen on the Modelling page). The project was successful in building the foundations for future teams to complete the synthesis of photosystem II in E. coli. This provides a significant leap into the development of a hydrogen-generating bacterial system and a renewable biological energy source. Our policy and practice initiatives were also successful for increasing public awareness of the global energy crisis and the potential synthetic biology has to offer in providing a solution (which can be seen on the Policy & Practice page).
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