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Team:Goettingen/proLP
From 2014.igem.org
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<h3>Overview</h3> | <h3>Overview</h3> | ||
<h4><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR">PCR Methods</a></h4> | <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR">PCR Methods</a></h4> | ||
<ul> | <ul> | ||
| - | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#Pfus_PCR"> | + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#Pfus_PCR"><i>PfuS</i> PCR</a></li> |
| - | + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#Fusi_PCR">Fusion PCR</a></li> | |
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#E_col"><i>E.coli</i> colony PCR</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#Y_col">Yeast colony PCR</a></li> | ||
</ul> | </ul> | ||
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<h4><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con">Plasmid Construction</a></h4> | <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con">Plasmid Construction</a></h4> | ||
<ul> | <ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#rest_DNA">Restriction of DNA</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#liga_DNA">Ligation of DNA fragments</a></li> | ||
<li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#BP">BP recombination reaction</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#BP">BP recombination reaction</a></li> | ||
<li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#LR">LR recombination reaction</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#LR">LR recombination reaction</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#seam_clone">SEAMLESS Cloning</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#LB">Peptide Library construction</a></li> | ||
</ul> | </ul> | ||
<h4><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran">Plasmid Transformation</a></h4> | <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran">Plasmid Transformation</a></h4> | ||
<ul> | <ul> | ||
| - | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#"><i>E.coli</i> | + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#E_comp"><i>E.coli</i> competent cells</a></li> |
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#E_plas">Plasmid isolation (<i>E.coli</i>)</a></li> | ||
<li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#E_tran"><i>E.coil</i> transformation</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#E_tran"><i>E.coil</i> transformation</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#Y_iso">Plasmid isolation (Yeast)</a></li> | ||
<li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#Y_tran">Yeast transformation</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#Y_tran">Yeast transformation</a></li> | ||
</ul> | </ul> | ||
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<h4><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony">Colony Scanning</a></h4> | <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony">Colony Scanning</a></h4> | ||
<ul> | <ul> | ||
<li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#E_cra"><i>E.coli</i> cracking</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#E_cra"><i>E.coli</i> cracking</a></li> | ||
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<li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Auto_act">Auto-activity Test</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Auto_act">Auto-activity Test</a></li> | ||
| - | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony# | + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Y2H_robot">Yeast-Two-Hybrid Assay with Robot</a></li> |
| - | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony# | + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Y2H_screen">Yeast-Two-Hybrid Screening</a></li> |
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</ul> | </ul> | ||
| + | <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_protein">Protein Assessment</a></li></h4> | ||
| + | <ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#SDS">SDS PAGE</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#Coo">Coomassie Staining</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#P_Exp">Protein Expression</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#Puri">Protein Purification</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#Dialysis">Protein Dialysis </a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#Bradford">Bradford Assay</a></li> | ||
| + | </ul> | ||
| + | <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_inVivo"><i>In vivo</i> tests</a></li></h4> | ||
| + | <ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_inVivo#Prod">Production of spore suspension</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_inVivo#Culti_A">Cell cultivation</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_inVivo#immu">Immunofluorescence Staining </a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_inVivo#Calc">Calcofluor staining</a></li> | ||
| + | </ul> | ||
</div> | </div> | ||
</html> | </html> | ||
Latest revision as of 21:17, 17 October 2014
Overview
PCR Methods
Plasmid Construction
- Restriction of DNA
- Ligation of DNA fragments
- BP recombination reaction
- LR recombination reaction
- SEAMLESS Cloning
- Peptide Library construction
Plasmid Transformation
- E.coli competent cells
- Plasmid isolation (E.coli)
- E.coil transformation
- Plasmid isolation (Yeast)
- Yeast transformation
