Team:Cambridge-JIC/Guide/Constructs/CodingSequences

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If possible, codon optimise your sequence for use in plants, or preferably for use with MP. A [codon table] for MP produced from predicted ORFs is available on this wiki.
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== Coding Sequences ==
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If possible, codon optimise your sequence for use in plants, or preferably for use with Marchantia polymorpha. A [[Team:Cambridge-JIC/Marchantia/Codon | codon table]] for Marchantia produced from predicted ORFs is available on this wiki.
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There is evidence of considerable spice sites in the MP genome, so there is no guarantee that a coding sequence that works in other chassis will be correctly translated in MP.
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There is evidence of a considerable number splice sites in the MP genome, so there is no guarantee that a coding sequence that works in other chassis will be correctly translated in Marchantia.  
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Best to try!
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Latest revision as of 15:25, 17 October 2014

Cambridge iGEM 2014


Coding Sequences

If possible, codon optimise your sequence for use in plants, or preferably for use with Marchantia polymorpha. A codon table for Marchantia produced from predicted ORFs is available on this wiki.

There is evidence of a considerable number splice sites in the MP genome, so there is no guarantee that a coding sequence that works in other chassis will be correctly translated in Marchantia.

Best to try!