Latest revision as of 22:45, 17 October 2014

Team:WPI-Worcester - 2014.igem.org

"

Team:WPI-Worcester

From 2014.igem.org

Future Applications

While CAEV was a good starting point, our project can be applied to virtually any other disease. The only requirements for using this construct as a diagnostic tool are that the disease to be diagnosed is caused by a pathogen, the pathogen produces a known antigen, and the host organism produces antibodies to the antigen. Once the target disease is chosen, the sequence for the antigen can easily be placed into the construct in CAEV’s place to create E. coli that produce the antigen of interest on the surface of the membrane. This can be used to diagnose the disease in the same agglutination test designed for the original project by combining the bacteria with plasma from the host organism in a 96-well plate and letting it sit overnight. Currently, our team has identified two diseases that could benefit greatly from our diagnostic tool: Ebola, and dengue fever.

Ebola is difficult to diagnose because it can take an infected individual up to 21 days to start displaying symptoms^[1], and once shown, initial symptoms are ubiquitous, resembling the flu^[2]. Being able to diagnose Ebola early and accurately would allow doctors to isolate the individual and provide therapeutic treatments sooner, both decreasing the likeliness of the virus spreading and increasing the chances that someone infected will survive.

The symptoms of dengue fever also resemble those of other illnesses, and it is easily confused with malaria and typhoid fever^[3]. There is no known cure for dengue fever, and the only effective treatment currently known is fluid replacement therapy, which only works if the disease is detected very early^[4]. While it is possible to diagnose dengue fever, the test results often come back when it is already too late to help the patient^[3]. Our diagnostic tool could fix this problem, as the only things required would be the E. coli expressing dengue fever antigens and a blood sample from a potentially infected individual, allowing a diagnostic result to be determined overnight.

References

[1] http://www.cdc.gov/vhf/ebola/symptoms/index.html

[2] http://www.cdc.gov/vhf/ebola/diagnosis/index.html

[3] http://www.mayoclinic.org/diseases-conditions/dengue-fever/basics/tests-diagnosis/con-20032868

[4] http://www.cdc.gov/dengue/symptoms/index.html

@@ Line 4: / Line 4: @@
 		<meta name="generator" content="MediaWiki 1.16.5" />
 <link rel="shortcut icon" href="/favicon.ico" />
-<link rel="search" type="application/opensearchdescription+xml" href="/wiki/opensearch_desc.php" title="2013.igem.org (en)" />
+<link rel="search" type="application/opensearchdescription+xml" href="/wiki/opensearch_desc.php" title="2014.igem.org (en)" />
 <link title="Creative Commons" type="application/rdf+xml" href="/wiki/index.php?title=Team:WPI-Worcester&amp;action=creativecommons" rel="meta" />
 <link rel="copyright" href="http://creativecommons.org/licenses/by/3.0/" />
-<link rel="alternate" type="application/atom+xml" title="2013.igem.org Atom feed" href="/wiki/index.php?title=Special:RecentChanges&amp;feed=atom" />		<title>Team:WPI-Worcester - 2014.igem.org</title>
+<link rel="alternate" type="application/atom+xml" title="2014.igem.org Atom feed" href="/wiki/index.php?title=Special:RecentChanges&amp;feed=atom" />		<title>Team:WPI-Worcester - 2014.igem.org</title>
 		<style type="text/css" media="screen, projection">/*<![CDATA[*/
 			@import "/wiki/skins/common/shared.css?270";
 			@import "/wiki/skins/igem/main.css?270";
-		/*]]>*/</style>
+		/*]]>*/
+		#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading,.visualClear {display: none;}
+</style>
 		<link rel="stylesheet" type="text/css" media="print" href="/wiki/skins/common/commonPrint.css?270" />
 		<!--[if lt IE 5.5000]><style type="text/css">@import "/wiki/skins/igem/IE50Fixes.css?270";</style><![endif]-->
@@ Line 30: / Line 32: @@
 wgVariantArticlePath=false,
 wgActionPaths={},
-wgServer="https://2013.igem.org",
+wgServer="https://2014.igem.org",
 wgCanonicalNamespace="",
 wgCanonicalSpecialPageName=false,
@@ Line 51: / Line 53: @@
 wgDigitTransformTable=["", ""],
 wgMainPageTitle="Main Page",
-wgFormattedNamespaces={"-2": "Media", "-1": "Special", "0": "", "1": "Talk", "2": "User", "3": "User talk", "4": "2013.igem.org", "5": "2013.igem.org talk", "6": "File", "7": "File talk", "8": "MediaWiki", "9": "MediaWiki talk", "10": "Template", "11": "Template talk", "12": "Help", "13": "Help talk", "14": "Category", "15": "Category talk"},
+wgFormattedNamespaces={"-2": "Media", "-1": "Special", "0": "", "1": "Talk", "2": "User", "3": "User talk", "4": "2014.igem.org", "5": "2014.igem.org talk", "6": "File", "7": "File talk", "8": "MediaWiki", "9": "MediaWiki talk", "10": "Template", "11": "Template talk", "12": "Help", "13": "Help talk", "14": "Category", "15": "Category talk"},
-wgNamespaceIds={"media": -2, "special": -1, "": 0, "talk": 1, "user": 2, "user_talk": 3, "2013.igem.org": 4, "2013.igem.org_talk": 5, "file": 6, "file_talk": 7, "mediawiki": 8, "mediawiki_talk": 9, "template": 10, "template_talk": 11, "help": 12, "help_talk": 13, "category": 14, "category_talk": 15, "image": 6, "image_talk": 7},
+wgNamespaceIds={"media": -2, "special": -1, "": 0, "talk": 1, "user": 2, "user_talk": 3, "2014.igem.org": 4, "2014.igem.org_talk": 5, "file": 6, "file_talk": 7, "mediawiki": 8, "mediawiki_talk": 9, "template": 10, "template_talk": 11, "help": 12, "help_talk": 13, "category": 14, "category_talk": 15, "image": 6, "image_talk": 7},
-wgSiteName="2013.igem.org",
+wgSiteName="2014.igem.org",
 wgCategories=[],
-wgMWSuggestTemplate="https://2013.igem.org/wiki/api.php?action=opensearch\x26search={searchTerms}\x26namespace={namespaces}\x26suggest",
+wgMWSuggestTemplate="https://2014.igem.org/wiki/api.php?action=opensearch\x26search={searchTerms}\x26namespace={namespaces}\x26suggest",
-wgDBname="2013_igem_org",
+wgDBname="2014_igem_org",
 wgSearchNamespaces=[0],
 wgMWSuggestMessages=["with suggestions", "no suggestions"],
@@ Line 91: / Line 93: @@
 	<div id="search-controls" class="noprint">
 		<form action="/Special:Search" id="searchform">
-		<input id="searchInput" name="search" type="text" title="Search 2013.igem.org [f]" accesskey="f" value="" />
+		<input id="searchInput" name="search" type="text" title="Search 2014.igem.org [f]" accesskey="f" value="" />
 			 <input type='submit' name="go" class="searchButton" id="searchGoButton"	value="Go" title="Go to a page with this exact name if exists" />&nbsp;
 		       <input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" />
@@ Line 101: / Line 103: @@
 				<h1 class="firstHeading">Team:WPI-Worcester</h1>
 		<div id="bodyContent">
-			<h3 id="siteSub" class='noprint'>From 2013.igem.org</h3>
+			<h3 id="siteSub" class='noprint'>From 2014.igem.org</h3>
 			<div id="contentSub"></div>
 						<!--
@@ Line 153: / Line 155: @@
 H8 {
-     	  font-family: Georgia;
+     	  font-family: Arial;
      	  text-transform: none;
      	  text-decoration: none;
      	  text-align: left;
-     	  color: #AC2B37;
+     	  color: #000000;
-     	  font-size: 12pt;
+     	  font-size: 10pt;
+}
 H9 {
      	  font-family: Georgia;
@@ Line 165: / Line 167: @@
      	  text-decoration: none;
      	  text-align: left;
-     	  color: #AC2B37;
+     	  color: #A9b0b7;
-     	  font-size: 14pt;
+     	  font-size: 16pt;
      }
@@ Line 564: / Line 566: @@
 			<li><a href="https://2014.igem.org/Team:WPI-Worcester/Team">Bios</a></li>
 			<li><a href="https://2014.igem.org/Team:WPI-Worcester/Team-Gallery">Team Gallery</a></li>
+			<li><a href="https://igem.org/Team.cgi?id=1423">Official Team Page</a></li>
 		</ul>
 	</li>
@@ Line 569: / Line 572: @@
 	<li><a href="#"><center><img src="https://static.igem.org/mediawiki/2014/4/41/WPI_Project_Link.png"/></center><p>Project</p></a>
 		<ul>
-	    <li><a href="https://2014.igem.org/Team:WPI-Worcester/Overview">Project Overview and Abstract</a></li>
+ <li><a href="https://2014.igem.org/Team:WPI-Worcester/Motivation">Motivation</a></li>
              <li><a href="https://2014.igem.org/Team:WPI-Worcester/Background">Background</a></li>
-            <li><a href="https://2014.igem.org/Team:WPI-Worcester/Motivation">Motivation</a></li>
+	    <li><a href="https://2014.igem.org/Team:WPI-Worcester/Overview">Project Overview</a></li>
 	    <li><a href="https://2014.igem.org/Team:WPI-Worcester/Future-Applications">Future Applications</a></li>
@@ Line 581: / Line 584: @@
 			<li><a href="https://2014.igem.org/Team:WPI-Worcester/Our-Construct">Our Construct</a></li>
 			<li><a href="https://2014.igem.org/Team:WPI-Worcester/Proof-of-Principle">Proof of Principle</a></li>
-			<li><a href="https://2014.igem.org/Team:WPI-Worcester/ATF1">Biobrick Characterization</a></li>
+			<li><a href="https://2014.igem.org/Team:WPI-Worcester/ATF1">Better BioBrick Characterization</a></li>
-			<li><a href="https://2014.igem.org/Team:WPI-Worcester/Biobricks">Biobricks</a></li>
+			<li><a href="https://2014.igem.org/Team:WPI-Worcester/Biobricks">BioBricks</a></li>
 			<li><a href="https://2014.igem.org/Team:WPI-Worcester/Medal-Fulfillment">Medal Fulfillment</a></li>
@@ Line 588: / Line 591: @@
 	</li>
-	<li><a href="#"><center><img src="https://static.igem.org/mediawiki/2014/7/7e/WPI_Notes_Link.png"/></center></<p></p><p>Notebook</p></a>
+	<li><a href="#"><center><img src="https://static.igem.org/mediawiki/2014/7/7e/WPI_Notes_Link.png"/></center><p>Notebook</p></a>
 		<ul>
 			<li><a href="https://2014.igem.org/Team:WPI-Worcester/Notebook">Weekly Log</a></li>
@@ Line 599: / Line 602: @@
 	<li><a href="#"><center><img src="https://static.igem.org/mediawiki/2014/d/d4/WPI_Safety_Link.png"/></center><p>Practices</p></a>
 		<ul>
+                <li><a href="https://2014.igem.org/Team:WPI-Worcester/Outreach">Outreach</a></li>
              <li><a href="https://2014.igem.org/Team:WPI-Worcester/Survey">Survey</a></li>
-            <li><a href="https://2013.igem.org/Team:WPI-Worcester/Outreach">Outreach</a></li>
   		<li><a href="https://2014.igem.org/Team:WPI-Worcester/Collaborations">Collaborations</a></li>
 		<li><a href="https://2014.igem.org/Team:WPI-Worcester/Interlab">Interlab Study</a></li>
@@ Line 621: / Line 624: @@
 </body>
-<p></p>
+</br>
-<p></p>
+<h4>Future Applications</h4><p>While CAEV was a good starting point, our project can be applied to virtually any other disease. The only requirements for using this construct as a diagnostic tool are that the disease to be diagnosed is caused by a pathogen, the pathogen produces a known antigen, and the host organism produces antibodies to the antigen. Once the target disease is chosen, the sequence for the antigen can easily be placed into the construct in CAEV’s place to create <i>E. coli</i> that produce the antigen of interest on the surface of the membrane. This can be used to diagnose the disease in the same agglutination test designed for the original project by combining the bacteria with plasma from the host organism in a 96-well plate and letting it sit overnight. Currently, our team has identified two diseases that could benefit greatly from our diagnostic tool: Ebola, and dengue fever.</p>
-<p></p>
-<p></p>
-<p></p>
-<p></p>
-<h4>Future Applications</h4><p>While CAEV was a good starting point, our project can be applied to virtually any other disease. The only requirements for using this construct as a diagnostic tool are that the disease to be diagnosed is caused by a pathogen, the pathogen produces a known antigen, and the host organism produces antibodies to the antigen. Once the target disease is chosen, the sequence for the antigen can easily be placed into the construct in CAEV’s place to create E. coli that produce the antigen of interest on the surface of the membrane. This can be used to diagnose the disease in the same agglutination test designed for the original project by combining the bacteria with plasma from the host organism in a 96-well plate and letting it sit overnight. Currently, our team has identified two diseases that could benefit greatly from our diagnostic tool: Ebola, dengue fever.</p>
 <p><center><img src="https://static.igem.org/mediawiki/2014/thumb/6/66/WPI_GeneralAntigen.png/800px-WPI_GeneralAntigen.png"/></p></center>
-<p>Ebola is difficult to diagnose because it can take an infected individual up to 21 days to start displaying symptoms1, and once shown, initial symptoms are ubiquitous, resembling the flu2. Being able to diagnose Ebola early and accurately would allow doctors to isolate the individual and provide therapeutic treatments sooner, both decreasing the likeliness of the virus spreading and increasing the chances that someone infected will survive.</p>
+<p>Ebola is difficult to diagnose because it can take an infected individual up to 21 days to start displaying symptoms<sup>[1]</sup>, and once shown, initial symptoms are ubiquitous, resembling the flu<sup>[2]</sup>. Being able to diagnose Ebola early and accurately would allow doctors to isolate the individual and provide therapeutic treatments sooner, both decreasing the likeliness of the virus spreading and increasing the chances that someone infected will survive.</p>
-<p>The symptoms of dengue fever also resemble those of other illnesses, and it is easily confused with malaria and typhoid fever3. There is no known cure for dengue fever, and the only effective treatment currently known is fluid replacement therapy, which only works if the disease is detected very early4.  While it is possible to diagnose dengue fever, the test results often come back when it is already too late to help the patient3. Our diagnostic tool could fix this problem, as the only things required would be the  E. coli expressing dengue fever antigens and a blood sample from a potentially infected individual, allowing a diagnostic result to be determined overnight.
+<p>The symptoms of dengue fever also resemble those of other illnesses, and it is easily confused with malaria and typhoid fever<sup>[3]</sup>. There is no known cure for dengue fever, and the only effective treatment currently known is fluid replacement therapy, which only works if the disease is detected very early<sup>[4]</sup>.  While it is possible to diagnose dengue fever, the test results often come back when it is already too late to help the patient<sup>[3]</sup>. Our diagnostic tool could fix this problem, as the only things required would be the  <i>E. coli</i> expressing dengue fever antigens and a blood sample from a potentially infected individual, allowing a diagnostic result to be determined overnight.
 </p>
+</br>
+<p><h9>References</h9></p>
+<h8><p>[1] http://www.cdc.gov/vhf/ebola/symptoms/index.html</p>
+<p>[2] http://www.cdc.gov/vhf/ebola/diagnosis/index.html</p>
+<p>[3] http://www.mayoclinic.org/diseases-conditions/dengue-fever/basics/tests-diagnosis/con-20032868</p>
+<p>[4] http://www.cdc.gov/dengue/symptoms/index.html</p></h8>
+</br>
+</br>
+</br>
 </html>