Team:Zamorano/Biosecurity

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      <li><a href="https://2014.igem.org/Team:Zamorano/Overview">Overview</a></li>
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<div id="tab-description"><p> <h1>Risk evaluation of the E. coli bacteria modified with genes of fluorescence</h1></p><br />
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<div id="tab-description"><p> <h1>Risk assessment of the <i>E. coli </i> bacteria modified with genes of fluorescence</h1></p><br />
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<ul>
<ul>
<li class=""><a href="https://2014.igem.org/Team:Zamorano/Riskevaluation">Introduction</a></li>
<li class=""><a href="https://2014.igem.org/Team:Zamorano/Riskevaluation">Introduction</a></li>
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<li class=""><a href="https://2014.igem.org/Team:Zamorano/ERAguide">ERA guide</a></li>
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<li class=""><a href="https://2014.igem.org/Team:Zamorano/RiskMethodology">Methodology</a></li>
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<li class="active"><a href="https://2014.igem.org/Team:Zamorano/Biosecurity">Biosecurity</a></li>
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<li class="active"><a href="https://2014.igem.org/Team:Zamorano/Biosecurity">Biosafety</a></li>
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<li class=""><a href="https://2014.igem.org/Team:Zamorano/Presentation">Presentation to NCBB</a></li>
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<li class=""><a href="https://2014.igem.org/Team:Zamorano/Presentation">Achievements</a></li>
<li class=""><a href="https://2014.igem.org/Team:Zamorano/Bibliography">Bibliography</a></li>
<li class=""><a href="https://2014.igem.org/Team:Zamorano/Bibliography">Bibliography</a></li>
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</ul>
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<br /><p><h1>Biosecurity</h1></p><br />
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<br /><p><h1>Biosafety</h1></p><br />
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<p>Good security practices (GSP) were taken of a publishing of the worldwide health organization (WHO) titled “Biosecurity manual in the lab” in the 2005 and adapted to the practice made. This guaranteed the entire safety of the staff members that worked in the modification and testing of the bacteria.  
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<h2>What is biosafety?</h2>
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It is important to mention that although the organism does not represent a biologic risk, for the development of this Project it was necessary to operate under a biosecurity level 2 with the intention of learning how to apply better the GSP. In order to achieve this objective the practice was based in 4 fundamental axes of biosecurity.</p><br />
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<p>Good Security Practices (GSP) were taken out of a publication made by the Worldwide Health Organization (WHO) titled <i>“Biosecurity manual in the lab”</i> in 2005, and adapted it to the practice performed. This guaranteed the safety of the staff members that worked in the modification and testing of the bacterium. It is important to mention that the organism does not represent a biologic risk because of it been non-pathogenic. None the less, for the development of this project it was necessary to operate under a biosafety level 2 with the intention of learning how to apply better GSPs. In order to achieve this objective, the practice was based in 4 fundamental axes of biosafety:</p><br />
<ul>
<ul>
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<li>Access restricted to people not authorized. </li>
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<li><p>Restricted access for people not authorized</p> </li>
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<li>Personal protection equipment team manages the instruments in the lab.</li>
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<li><p>Personal protection equipment team manages the instruments in the lab.</p></li>
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<li>Biocontention mechanisms.</li>
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<li><p>Biocontention mechanisms.</p></li>
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<li>Final disposal methods.</li>
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<li><p>Final disposal methods.</p></li>
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<p><h2>Why is biosafety so important?</h2></p>
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    <td width="72" nowrap="nowrap" style="width:54.1pt;padding:0cm 3.5pt 0cm 3.5pt;height:8.05pt;"></td>
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<p>The use of biological agents is nothing new. History gives us many examples even before microbiology science was developed. Recently, there were innovative and perverted strategies meant to cause panic. Examples include the dissemination of anthrax spores (<i>Bacillus anthracis</i>) in 2001.</p>
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    <td width="86" nowrap="nowrap" style="width:64.2pt;padding:0cm 3.5pt 0cm 3.5pt;height:8.05pt;"></td>
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    <td width="451" nowrap="nowrap" colspan="4" style="width:338.5pt;background:#EEECE1;padding:0cm 3.5pt 0cm 3.5pt;height:8.05pt;" bgcolor="#EEECE1"><p class="MsoNormal" align="center" style="text-align:center;"><strong><span style="font-family:'Book Antiqua','serif'; font-size:12.0pt; color:black; ">Risk estimation</span></strong></p></td>
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<p><h2>Bioterrorism and its effect</h2></p>
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<p>Bioterrorism is defined as criminal actions using biological agents such as pathogenic microorganisms, toxins or lethal substances in order to spread diseases, cause panic situations and, in some cases, death.</p>
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  <tr style="height:11.0pt;">
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<p>Considering possible attacks and a worldwide terrorist crusade, international organizations, address extraordinary financial resources and military logistics in order to create new defense protocols and to promote legislation against this menace in member countries. In order to be in the position to give an effective and timely wise response to the threat of biological weapons, governments have the duty to safeguard their populations.</p>
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    <td width="72" nowrap="nowrap" rowspan="4" style="width:54.1pt;border:solid white 1.0pt;background:#EEECE1;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#EEECE1"><p class="MsoNormal" align="center" style="text-align:center;"><strong><span style="font-family:'Book Antiqua','serif'; font-size:12.0pt; color:black; ">Probability</span></strong></p></td>
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    <td width="86" nowrap="nowrap" style="width:64.2pt;border:solid white 1.0pt;border-left:none;background:#EEECE1;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#EEECE1"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:black; ">VERY    HIGH</span></p></td>
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<p><h2>What have other done to address this issue?</h2></p>
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    <td width="125" nowrap="nowrap" style="width:93.75pt;border:solid white 1.0pt;border-left:none;background:#C4D79B;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#C4D79B"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:black; ">LOW</span></p></td>
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<p>Organizations such as the UN, WHO and FAO, among others, have written international regulations in order to take care of transportation, management and adequate use of infectious substances. Also, national governments have adopted safety measures and written internal regulations in order to protect its populations.</p>
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    <td width="125" nowrap="nowrap" style="width:93.75pt;border:solid white 1.0pt;border-left:none;background:#FDE9D9;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#FDE9D9"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:black; ">MODERATE</span></p></td>
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    <td width="106" nowrap="nowrap" style="width:79.5pt;border:solid white 1.0pt;border-left:none;background:#FABF8F;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#FABF8F"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:black; ">HIGH</span></p></td>
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<p><h2>How should Synthetic Biology be introduced in Latin American countries?</h2></p>
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    <td width="95" nowrap="nowrap" style="width:71.5pt;border:solid white 1.0pt;border-left:none;background:#FABF8F;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#FABF8F"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:black; ">HIGH</span></p></td>
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<p>Activities related to synthetic biology must be implemented progressively, step by step, in such a manner that risks are evaluated in terms of protection to human and environmental health, and once these have been assessed, it is possible to go to the next level with reasonable security. In addition, any issues must be addressed in an individual fashion, case by case. In addition, there are new kinds of conflicts, and its evaluation should be done with the same criteria applied to ethical and legal analysis of other emerging biotechnologies and not away from them.</p>
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  </tr>
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<p>If you want more information <a target="_blank" href="https://static.igem.org/mediawiki/2014/c/ce/Zamorano_Education_Bioterrorism.pdf" style="text-decoration:underline;">clic here</a></p>
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  <tr style="height:11.0pt;">
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    <td width="86" nowrap="nowrap" style="width:64.2pt;border-top:none;border-left:none;border-bottom:solid white 1.0pt;border-right:solid white 1.0pt;background:#EEECE1;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#EEECE1"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:black; ">HIGH</span></p></td>
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    <td width="125" nowrap="nowrap" style="width:93.75pt;border:none;border-right:solid white 1.0pt;background:#C4D79B;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#C4D79B"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:black; ">LOW</span></p></td>
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-
    <td width="125" nowrap="nowrap" style="width:93.75pt;border-top:none;border-left:none;border-bottom:solid white 1.0pt;border-right:solid white 1.0pt;background:#C4D79B;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#C4D79B"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:black; ">LOW</span></p></td>
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    <td width="106" nowrap="nowrap" style="width:79.5pt;border-top:none;border-left:none;border-bottom:solid white 1.0pt;border-right:solid white 1.0pt;background:#FDE9D9;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#FDE9D9"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:black; ">MODERATE</span></p></td>
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    <td width="95" nowrap="nowrap" style="width:71.5pt;border-top:none;border-left:none;border-bottom:solid white 1.0pt;border-right:solid white 1.0pt;background:#FABF8F;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#FABF8F"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:black; ">HIGH</span></p></td>
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-
  </tr>
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  <tr style="height:11.0pt;">
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    <td width="86" nowrap="nowrap" style="width:64.2pt;border:none;border-bottom:solid white 1.0pt;background:#FFFF99;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#FFFF99"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:black; ">LOW</span></p></td>
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    <td width="125" nowrap="nowrap" style="width:93.75pt;border:solid red 1.5pt;background:#B8CCE4;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#B8CCE4"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:red; ">INSIGNIFICANT</span></p></td>
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    <td width="125" nowrap="nowrap" style="width:93.75pt;border-top:none;border-left:none;border-bottom:solid white 1.0pt;border-right:solid white 1.0pt;background:#C4D79B;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#C4D79B"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:black; ">LOW</span></p></td>
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-
    <td width="106" nowrap="nowrap" style="width:79.5pt;border-top:none;border-left:none;border-bottom:solid white 1.0pt;border-right:solid white 1.0pt;background:#FDE9D9;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#FDE9D9"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:black; ">MODERATE</span></p></td>
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-
    <td width="95" nowrap="nowrap" style="width:71.5pt;border-top:none;border-left:none;border-bottom:solid white 1.0pt;border-right:solid white 1.0pt;background:#FDE9D9;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#FDE9D9"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:black; ">MODERATE</span></p></td>
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-
  </tr>
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-
  <tr style="height:11.0pt;">
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-
    <td width="86" nowrap="nowrap" style="width:64.2pt;border:none;border-bottom:solid white 1.0pt;background:#FFFF99;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#FFFF99"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:black; ">VERY    LOW</span></p></td>
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-
    <td width="125" nowrap="nowrap" style="width:93.75pt;border:solid red 1.5pt;border-top:none;background:#B8CCE4;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#B8CCE4"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:red; ">INSIGNIFICANT</span></p></td>
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    <td width="125" nowrap="nowrap" style="width:93.75pt;border-top:none;border-left:none;border-bottom:solid white 1.0pt;border-right:solid white 1.0pt;background:#B8CCE4;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#B8CCE4"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:black; ">INSIGNIFICANT</span></p></td>
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-
    <td width="106" nowrap="nowrap" style="width:79.5pt;border-top:none;border-left:none;border-bottom:solid white 1.0pt;border-right:solid white 1.0pt;background:#C4D79B;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#C4D79B"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:black; ">LOW</span></p></td>
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    <td width="95" nowrap="nowrap" style="width:71.5pt;border-top:none;border-left:none;border-bottom:solid white 1.0pt;border-right:solid white 1.0pt;background:#FDE9D9;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#FDE9D9"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:black; ">MODERATE</span></p></td>
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-
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  <tr style="height:10.55pt;">
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    <td width="72" nowrap="nowrap" style="width:54.1pt;border:solid white 1.0pt;border-top:none;padding:0cm 3.5pt 0cm 3.5pt;height:10.55pt;"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:black; ">&nbsp;</span></p></td>
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    <td width="86" nowrap="nowrap" style="width:64.2pt;border:none;border-bottom:solid white 1.0pt;padding:0cm 3.5pt 0cm 3.5pt;height:10.55pt;"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:black; ">&nbsp;</span></p></td>
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    <td width="125" nowrap="nowrap" style="width:93.75pt;border:solid white 1.0pt;border-top:none;background:#FFFF99;padding:0cm 3.5pt 0cm 3.5pt;height:10.55pt;" bgcolor="#FFFF99"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:black; ">MARGINAL</span></p></td>
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    <td width="125" nowrap="nowrap" style="width:93.75pt;border-top:none;border-left:none;border-bottom:solid white 1.0pt;border-right:solid white 1.0pt;background:#F2F2F2;padding:0cm 3.5pt 0cm 3.5pt;height:10.55pt;" bgcolor="#F2F2F2"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:black; ">MINOR</span></p></td>
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    <td width="106" nowrap="nowrap" style="width:79.5pt;border-top:none;border-left:none;border-bottom:solid white 1.0pt;border-right:solid white 1.0pt;background:#F2F2F2;padding:0cm 3.5pt 0cm 3.5pt;height:10.55pt;" bgcolor="#F2F2F2"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:black; ">INTERMEDIA</span></p></td>
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    <td width="95" nowrap="nowrap" style="width:71.5pt;border-top:none;border-left:none;border-bottom:solid white 1.0pt;border-right:solid white 1.0pt;background:#F2F2F2;padding:0cm 3.5pt 0cm 3.5pt;height:10.55pt;" bgcolor="#F2F2F2"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:black; ">MAYOR</span></p></td>
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    <td width="86" nowrap="nowrap" style="width:64.2pt;border:none;border-bottom:solid white 1.0pt;padding:0cm 3.5pt 0cm 3.5pt;height:8.05pt;"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:black; ">&nbsp;</span></p></td>
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    <td width="451" nowrap="nowrap" colspan="4" style="width:338.5pt;border:solid white 1.0pt;border-top:none;background:#EEECE1;padding:0cm 3.5pt 0cm 3.5pt;height:8.05pt;" bgcolor="#EEECE1"><p class="MsoNormal" align="center" style="text-align:center;"><strong><span style="font-family:'Book Antiqua','serif'; font-size:12.0pt; color:black; ">CONSEQUENCE</span></strong></p></td>
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<p style="text-align:center;"><b>Thanks to the GSP and other basic measures of biosecurity the happening probability estimation and the consequence of this to happen be meaningless.</b></p>
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Latest revision as of 03:04, 18 October 2014

Risk assessment of the E. coli bacteria modified with genes of fluorescence



Biosafety


What is biosafety?

Good Security Practices (GSP) were taken out of a publication made by the Worldwide Health Organization (WHO) titled “Biosecurity manual in the lab” in 2005, and adapted it to the practice performed. This guaranteed the safety of the staff members that worked in the modification and testing of the bacterium. It is important to mention that the organism does not represent a biologic risk because of it been non-pathogenic. None the less, for the development of this project it was necessary to operate under a biosafety level 2 with the intention of learning how to apply better GSPs. In order to achieve this objective, the practice was based in 4 fundamental axes of biosafety:


  • Restricted access for people not authorized

  • Personal protection equipment team manages the instruments in the lab.

  • Biocontention mechanisms.

  • Final disposal methods.


Why is biosafety so important?

The use of biological agents is nothing new. History gives us many examples even before microbiology science was developed. Recently, there were innovative and perverted strategies meant to cause panic. Examples include the dissemination of anthrax spores (Bacillus anthracis) in 2001.

Bioterrorism and its effect

Bioterrorism is defined as criminal actions using biological agents such as pathogenic microorganisms, toxins or lethal substances in order to spread diseases, cause panic situations and, in some cases, death.

Considering possible attacks and a worldwide terrorist crusade, international organizations, address extraordinary financial resources and military logistics in order to create new defense protocols and to promote legislation against this menace in member countries. In order to be in the position to give an effective and timely wise response to the threat of biological weapons, governments have the duty to safeguard their populations.

What have other done to address this issue?

Organizations such as the UN, WHO and FAO, among others, have written international regulations in order to take care of transportation, management and adequate use of infectious substances. Also, national governments have adopted safety measures and written internal regulations in order to protect its populations.

How should Synthetic Biology be introduced in Latin American countries?

Activities related to synthetic biology must be implemented progressively, step by step, in such a manner that risks are evaluated in terms of protection to human and environmental health, and once these have been assessed, it is possible to go to the next level with reasonable security. In addition, any issues must be addressed in an individual fashion, case by case. In addition, there are new kinds of conflicts, and its evaluation should be done with the same criteria applied to ethical and legal analysis of other emerging biotechnologies and not away from them.

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