Team:uOttawa

From 2014.igem.org

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             <a href="#" class="title"><span class="igem">iGEM</span>uOttawa</a>
             <a href="#" class="title"><span class="igem">iGEM</span>uOttawa</a>
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                 <a href="./project">project</a>
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                 <a href="/Team:uOttawa/project">project</a>
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                 <a href="/Team:uOttawa/drylab">dry lab</a>
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                 <a href="/Team:uOttawa/team">team</a>
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                         <h2>Take the tour!</h2>
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                         <h2>The project!</h2>
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                         <p>How we built the tri-stable switch in <em>S. cerevisae</em>.</p>
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                         <p>uOttawa iGEM has a project of epic proportions in store for this year.</p>
                         <p class="hover" hidden>Let us take you through our creative and scientific process of building one of the most interesting proposed genetic networks &mdash; one that may shed light on how stem cells specialize and allow us to create similar behaviour in a lab.</p>
                         <p class="hover" hidden>Let us take you through our creative and scientific process of building one of the most interesting proposed genetic networks &mdash; one that may shed light on how stem cells specialize and allow us to create similar behaviour in a lab.</p>
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                         <h2>The project</h2>
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                         <h2>Promoters</h2>
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                         <p>Modelling stem cell differentiation using a tri-stable switch.</p>
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                         <p>Eukaryotic, dual-input promoters.</p>
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                         <p class="hover" hidden>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Accusamus reprehenderit, minima consequuntur enim corporis quod quis unde iusto ratione consectetur! Quibusdam quas voluptas necessitatibus alias aspernatur quasi a maxime impedit.</p>
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                         <p class="hover" hidden>See our yeast-optimized inducible and repressible promoters that we used in our network.</p>
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                         <h3>Results</h3>
                         <h3>Results</h3>
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                         <h3>Methods</h3>
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                         <h3>Measurement</h3>
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                         <h3>Biobricks</h3>
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                         <h2>The tri-stable switch</h2>
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                         <h2>Modelling</h2>
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                         <p>Modelling stem cell differentiation in <em>S. cerevisae</em>.</p>
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                         <p>How to explain tri-stability with math</p>
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                         <p class="hover" hidden>Collaborating with Waterloo, we created different systems of differential equations to see if we can quantify how the tri-stable network behaves.</p>
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                         <h2>The tri-stable switch</h2>
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                         <h2>Equations</h2>
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                         <p>Modelling stem cell differentiation in <em>S. cerevisae</em>.</p>
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                         <p>and parameters</p>
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                        <p class="hover" hidden>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Accusamus reprehenderit, minima consequuntur enim corporis quod quis unde iusto ratione consectetur! Quibusdam quas voluptas necessitatibus alias aspernatur quasi a maxime impedit.</p>
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                         <h3>Results</h3>
                         <h3>Results</h3>
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                         <h3>Methods</h3>
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                         <h3>References</h3>
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                        <h3>Brick Builder</h3>
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                         <p>Modelling stem cell differentiation in <em>S. cerevisae</em>.</p>
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                         <p>Talking synbio to Ottawa's youth.</p>
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                         <h2>The tri-stable switch</h2>
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                         <h2>The Synthetic Biology course</h2>
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                         <p>Modelling stem cell differentiation in <em>S. cerevisae</em>.</p>
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                         <p>How to build a new university-level program for synthetic biology at uOttawa.</p>
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                         <h3>Results</h3>
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                         <h3>iGEM in the classroom</h3>
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                         <h3>Fairs and Festivals</h3>
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                         <h3>oGEM and SynBio Ontario</h3>
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                         <h3>Genetics and patent law</h3>
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                         <h3>Safety</h3>
                         <h3>Safety</h3>
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                     <img src="https://static.igem.org/mediawiki/2014/2/2c/Uo2014-tile-notebook.jpg" alt="">
                     <div class="text">
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                         <h3>Notebook</h3>
                         <h3>Notebook</h3>
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<center> Throughout the life cycle of any given cell, many pre-programmed genetic decisions are made by cells in order for the cells to develop normally or to respond appropriately to external stimuli. This is especially true in complex multi-cellular organisms, where various cell types have to work in tandem in a multitude of cellular tasks. For instance, the apoptotic pathway allows for controlled cell death, which is essential for the continued function of many multicellular organisms. Stem cells must make vital cellular decisions during early development of multicellular organisms about when to differentiate and into what cell type. Thus, it is through understanding the complexities of these ‘decisions’ that scientists may be able to design independent cells that will change its own cellular fate in order to better accomplish whatever its given task. With that in mind, the uOttawa iGEM team is attempting to recreate synthetically one particular cellular decision mechanism within a simply eukaryote (Saccharomyces cerevisiae), taking a step towards controlling entire decision pathways and possibly creating unique cell fates within existing cells.
 
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The mechanism behind pluripotency -- stem cells’ potential to differentiate into all different cell types-- has been a significant focus of modern research. In 2009, Sui Hang et al. theorized that pluripotency is controlled by a cascade of tri-stable switches, a type of genetic network motif that maintains three unique expression states simultaneously.  This year, the uOttawa iGEM team is constructing iGEM’s first functional tri-stable switch based upon this theoretical premise in order to ascertain the potential for tri-stable switches to function as a differentiation mechanism. By doing so, we hope to create genetically engineered cells that are capable of making autonomous cellular decision in reaction to a multitude of internal and external stimuli, allowing us to study this dynamic cellular decision mechanism.</center>
 
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<a href="https://2014.igem.org/Team:uOttawa"style="color:#000000">Home </a> </td>
 
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<a href="https://2014.igem.org/Team:uOttawa/Project"style="color:#000000"> Project</a></td>
 
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<p> Please be sure to keep these links, your audience will want to find your: </p>
 
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<li><a href="https://2014.igem.org/Team:uOttawa">Home</a> </li>
 
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<li><a href="https://2014.igem.org/Team:uOttawa/Team">Team</a> </li>
 
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<li><a href="https://igem.org/Team.cgi?year=2013&team_name=uOttawa">Official Team Profile</a> </li>
 
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<li><a href="https://2014.igem.org/Team:uOttawa/Project">Project</a> </li>
 
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<li><a href="https://2014.igem.org/Team:uOttawa/Parts">Parts</a> </li>
 
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<li><a href="https://2014.igem.org/Team:uOttawa/Modeling">Modeling</a> </li>
 
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<li><a href="https://2014.igem.org/Team:uOttawa/Notebook">Notebook</a> </li>
 
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<li><a href="https://2014.igem.org/Team:uOttawa/Safety">Safety</a> </li>
 
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<li><a href="https://2014.igem.org/Team:uOttawa/Attributions">Attributions</a> </li>
 
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<li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload">  2014.igem.org server</a>. </li>
 
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<li>All pages must be created under the team’s name space.</li>
 
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<li>As part of your documentation, keep the links from the menu to the left. </li>
 
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<li>Do not use flash in wiki code. </li>
 
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<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
 
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<p>We are currently working on providing teams with some easy to use design templates.
 
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<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
 
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<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
 
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<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
 
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<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
 
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<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
 
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<li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
 
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</ul>
 
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<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
 
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
 
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
 
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<li>Be clear about what you are doing and what you plan to do.</li>
 
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
 
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
 
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
 
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
 
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<li>Have lots of fun! </li>
 
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Latest revision as of 23:44, 17 October 2014

iGEMuOttawa

The project!

uOttawa iGEM has a project of epic proportions in store for this year.

Promoters

Eukaryotic, dual-input promoters.

Results

Measurement

Interlab study

Biobricks

Modelling

How to explain tri-stability with math

Equations

and parameters

Results

Brick Builder

Let's Talk Science

Talking synbio to Ottawa's youth.

The Synthetic Biology course

How to build a new university-level program for synthetic biology at uOttawa.

iGEM in the classroom

Fairs and Festivals

iGEM Mini-Enrichment

oGEM and SynBio Ontario

Genetics and patent law

The hardest-working team in iGEM.

Working hard Hardly working 24/7 since 2008.

Safety

Notebook

Collaborations

Retrieved from "http://2014.igem.org/Team:uOttawa"