Team:Imperial/Parts

From 2014.igem.org

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<html>
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<style type="text/css">
 
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#groupparts {text-align: center; margin-left: auto; margin-right: auto;}
 
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            <div class="pure-g">
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<tr>
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<td style="border:1px solid black;" colspan="3" align="center" height="150px" bgColor=#FF404B>
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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<br>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Imperial/Parts&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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                <div class="pure-u-1-1 main">
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                    <h1>Parts</h1>
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                    <div id="navWrap">
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                        <div id="subNav">
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                            <ul>
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                                <li><a data-scroll href="#featured">Featured Parts</a>
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                                </li>
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                                <li><a data-scroll href="#table">Table of Parts</a>
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                                </li>
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                            </ul>
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                        </div>
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                    </div>
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                    <section id="featured">
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                        <h2>Featured Parts</h2>
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                        <div>
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                            <h3><a href="http://parts.igem.org/Part:BBa_K1321340">BBa_K1321340</a></h3>
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                            <div>
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                                <div class="pure-g">
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                                    <div class="pure-u-1-3">
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                                        <p>N-terminal linker + double CBD</p>
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                                    </div>
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                                    <div class="pure-u-2-3">
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                                        <p>Double cellulose binding domain (dCBD) using two cellulose binding domains from <i>Trichoderma reesei</i> cellobiohydrolases, with an N-terminal linker and internal linker sequence between the two domains which are derived from the endogenous cellobiohydrolase linker sequence. This part is in RFC(25) Freiberg fusion format to allow for easy use in protein fusions. dCBD binding affinity for cellulose was <a href="https://2014.igem.org/Team:Imperial/Functionalisation"> characterised </a> using sfGFP fusions.
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</p>
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                                    </div>
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                                </div>
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                            </div>
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                        <h3><a href="http://parts.igem.org/Part:BBa_K1321339">BBa_K1321339</a></h3>
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                            <div>
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                                <div class="pure-g">
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                                    <div class="pure-u-1-3">
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                                        <p>CBDcenA + Linker</p>
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                                    </div>
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                                    <div class="pure-u-2-3">
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                                        <p>Cellulose binding domain (CBD) of Endoglucanase A (cenA) from <i>Cellulomonas fimi</i> with an endogenous C-terminal linker. The part is in Freiburg format (RFC 25) for ease of use in protein fusions.</p>
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                                    </div>
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                                </div>
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<a href="https://2014.igem.org/Team:Imperial"style="color:#000000">Home </a> </td>
 
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<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
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                            </div>
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<a href="https://2014.igem.org/Team:Imperial/Team"style="color:#000000"> Team </a> </td>
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                            <h3><a href="http://parts.igem.org/Part:BBa_K1321014"> BBa_K1321014</a></h3>
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                            <div>
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                                <div class="pure-g">
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                                    <div class="pure-u-1-3">
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                                        <p>CBDcipA with N and C-terminal linker </p>
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                                    </div>
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                                    <div class="pure-u-2-3">
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                                        <p>This CBD is from the Cellulosomal -scaffolding protein A (cipA) of <i>Clostridium thermocellum </i> including the endogenous linker sequences at the N and C-terminus. It is in RFC25 format to allow for easy use in protein fusions. At present the cloning for these constructs is still in progress to correct an illegal EcoRI site which was identified in the parts with this CBD. This can be achieved with a silent mutation via site-directed mutagenesis and we aim to send these parts to the registry once this is complete.
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</p>
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                                    </div>
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                                </div>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=Imperial"style="color:#000000"> Official Team Profile </a></td>
 
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<td style="border:1px solid black" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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                            </div>
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<a href="https://2014.igem.org/Team:Imperial/Project"style="color:#000000"> Project</a></td>
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                            <h3><a href="http://parts.igem.org/Part:BBa_K1321005">BBa_K1321005</a></h3>
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                            <div>
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                                <div class="pure-g">
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                                    <div class="pure-u-1-3">
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                                        <p>Synthetic Phytochelatin (PC) EC20</p>
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                                    </div>
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                                    <div class="pure-u-2-3">
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                                        <p>A general heavy metal-binding peptide consisting of 20 Glu-Cys (EC) repeats in Freiburg format (RFC[25]) to allow for easy use in fusion proteins. We created a library of CBD fusions with this part.
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</p>
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                                    </div>
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                                </div>
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                                </div>
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                                <h3><a href="http://parts.igem.org/Part:BBa_K1321200">BBa_K1321200</a></h3>
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                                <div>
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                                <div class="pure-g">
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                                    <div class="pure-u-1-3">
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                                        <p><i>Vitreoscilla</i> haemoglobin (VHb)</p>
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                                    </div>
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                                    <div class="pure-u-2-3">
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                                        <p>This part is a haemoglobin isolated from <i>Vitreoscilla</i> (VHb) which improves the metabolic function of obligate aerobes and facultative anaerobes in low-oxygen conditions. We used this part in <i> G. xylinus </i> to try and improve growth rate and cellulose production.
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</p>
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                                    </div>
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                                </div>
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                            </div>
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<a href="https://2014.igem.org/Team:Imperial/Parts"style="color:#000000"> Parts</a></td>
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                            <h3><a href="http://parts.igem.org/Part:BBa_K1321300">BBa_K1321300</a>, <a href="http://parts.igem.org/Part:BBa_K1321301">BBa_K1321301</a> </h3>
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                            <div>
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                                <div class="pure-g">
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                                    <div class="pure-u-1-3">
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                                        <p>pSEVA331 and pSEVA321 backbones</p>
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                                    </div>
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                                    <div class="pure-u-2-3">
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                                        <p>These are two broad host range vector backbones for which we have demonstrated use in <i>E. coli</i> and <i>Gluconacetobacter xylinus </i>.
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</p>
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                                    </div>
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                                </div>                           
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                            </div>
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                            <h3><a href="http://parts.igem.org/Part:BBa_K1321334"> BBa_K1321334</a>, <a href="http://parts.igem.org/Part:BBa_K1321335">BBa_K1321335</a></h3>
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                            <div>
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                                <div class="pure-g">
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                                    <div class="pure-u-1-3">
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                                        <p>Cellulose synthase operon AcsAB and AcsCD</p>
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                                    </div>
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                                    <div class="pure-u-2-3">
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                                        <p>Designed and refactored from <i>G. xylinus</i>, this operon was inserted into <i>E.coli </i> for cellulose synthesis.
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</p>
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                                    </div>
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                                </div>  
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</div>
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<a href="https://2014.igem.org/Team:Imperial/Modeling"style="color:#000000"> Modeling</a></td>
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                            <h3><a href="http://parts.igem.org/Part:BBa_K1321305">BBa_K1321305</a>,<a href="http://parts.igem.org/Part:BBa_K1321306"> BBa_K1321306</a></h3>
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                            <div>
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                                <div class="pure-g">
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                                    <div class="pure-u-1-3">
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                                        <p><i>Gluconacetobacter xylinus</i> strains ATCC53582 and Kombucha Isolate</p>
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                                    </div>
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                                    <div class="pure-u-2-3">
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                                        <p>We created and characterised a library of parts for this organism, with the aim to increase the accessibility the chassis for future work in iGEM. By sharing these strains and their genomes on the registry we hope this will contribute to their ease of use and characterisation.
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</p>
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                                    </div>
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                                </div>         
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                        </div>
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                </div>
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                </section>
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<a href="https://2014.igem.org/Team:Imperial/Notebook"style="color:#000000"> Notebook</a></td>
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                <section id="table">
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                    <h2>Table of Parts</h2>
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<a href="https://2014.igem.org/Team:Imperial/Safety"style=" color:#000000"> Safety </a></td>
 
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                </section>
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<a href="https://2014.igem.org/Team:Imperial/Attributions"style="color:#000000"> Attributions </a></td>
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            </div>
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<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
 
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<!--Parts Submitted to the Registry  -->
 
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<tr><td > <h3> Parts Submitted to the Registry </h3></td>
 
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<td ></td >
 
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<td > <h3>What information do I need to start putting my parts on the Registry? </h3></td>
 
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<p>
 
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An important aspect of the iGEM competition is the use and creation of standard  biological parts. Each team will make new parts during iGEM and will submit them to the <a href="http://partsregistry.org"> Registry of Standard Biological Parts</a>. The iGEM software provides an easy way to present the parts your team has created. The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox. 
 
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<p>
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    </div>
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<strong>Note that if you want to document a part you need to document it on the <a href="http://partsregistry.org Registry"> Registry</a>, not on your team wiki.</strong> Future teams and other users and are much more likely to find parts on the Registry than on your team wiki.
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Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without a need to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.
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<groupparts>iGEM014 Imperial</groupparts>
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{{:Team:Imperial/Templates:footer}}
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<h3>When should you put parts into the Registry?</h3>
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<p>
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As soon as possible! We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better recall you will have of all details surrounding your parts. Remember you don't need to send us the DNA to create an entry for a part on the Registry. However, you must send us the sample/DNA before the Jamboree. Only parts for which you have sent us samples/DNA are eligible for awards and medal requirements.
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<td > </td>
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The information needed to initially create a part on the Registry is:
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</p>
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<ol>
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<li>Part Name</li>
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<li>Part type</li>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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</ol>
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<p>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. Check out part <a href="http://parts.igem.org/Part:BBa_K404003">BBa_K404003</a> for an excellent example of a highly characterized part.
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</p>
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<p>
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You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry"> Add a Part to the Registry</a> link.
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<tr><td colspan="3" > <h3> Parts Table</h3></td></tr>
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<tr><td width="45%" colspan="3"  valign="top">
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Any parts your team has created will appear in this table below:</td></tr>
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<groupparts>iGEM013 Imperial</groupparts>
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Latest revision as of 03:59, 18 October 2014

Imperial iGEM 2014

Aqualose

Parts

Table of Parts

<groupparts>iGEM014 Imperial</groupparts>


Retrieved from "http://2014.igem.org/Team:Imperial/Parts"