Team:Cooper Union/OpenGel

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<ul class="menu">
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<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/TdT_project">De Novo Synthesis </a></li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/TdT_project">De Novo Synthesis </a></li>
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<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Telomere_project">Programmable Lifespan</a> </li>
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<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Telomere_project">Programmable Lifespan Timer</a> </li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Biohack_project">Biohacker Kit </a></li>
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<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Hardware">OpenSource Hardware </a> </li>
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<li class="menu-notebook">  Notebook  
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<li>Notch two evenly spaced slots into the acrylic square rods, this will be where the combs sit. The notch depth depends on your gel thickness but should be around 9mm</li>
<li>Notch two evenly spaced slots into the acrylic square rods, this will be where the combs sit. The notch depth depends on your gel thickness but should be around 9mm</li>
<li>Glue the square rods to the “gel_base” using the combs as alignment references</li>
<li>Glue the square rods to the “gel_base” using the combs as alignment references</li>
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<li>Glue lid pieces together and attach female banana plug</li>
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        <a href="http://www.cooper.edu" target="_blank" span="The Cooper Union"><img src="https://static.igem.org/mediawiki/2014/9/90/CU_2014_Cooper_union_logo.png" height="150" /></a>
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Latest revision as of 00:51, 18 October 2014

Cooper Union 2014 iGEM




Hardware



OpenGelBox

OpenRoller

OpenFuge

Microfluidics


OpenGelBox





The OpenGelBox is an easy-to-build midi gel box that can run up to 30 samples simultaneously. It is built using 6mm and 2mm acrylic sheets with a laser cut time of under a half hour. Previously, our lab would need to run several mini gels in series in order to analyze a large number of samples. With the OpenGelBox, we can more quickly analyze many samples while minimizing the use of consumable materials involved in gel electrophoresis.

Design

Plans




CAD Files

Parts

  • 6mm and 2mm acrylic sheet
  • 20x20mm acrylic square rod
  • 2 Male Banana plugs (or the your standardized plug)
  • Platinum wire (AWG 30)
  • Four M3x15 screws and nuts
  • Acrylic glue


Cut List


6mm sheet
  • Two “Sides”
  • One “base_plate”
  • Two “electrode_plate”
  • Two “front_back”
  • One “lid_top”
  • One “lid_electrode”
  • One “gel_base”
2mm sheet
  • Two “Comb”
  • Two 20x20x190mm acrylic square rods


Assembly


  1. Glue all plates, except the “electrode_plate”, together using acrylic glue. Clamps are recommended to ensure a watertight seal and better alignment.
  2. Attach male banana plugs to holes in the “sides” plate
  3. Attach the electrode wire to the power plugs and thread through the small hole in the “electrode_plate” so that the small hole is on the opposite side of the power plugs and the wire is behind the grate section
  4. Screw both “electrode_plate” pieces to their respective sides using M3 screws
  5. Notch two evenly spaced slots into the acrylic square rods, this will be where the combs sit. The notch depth depends on your gel thickness but should be around 9mm
  6. Glue the square rods to the “gel_base” using the combs as alignment references
  7. Glue lid pieces together and attach female banana plug



Open Source Midi Gel box

Gel caster

Gel caster and Comb


Projects Social Notebook Team

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