Team:NJAU China

From 2014.igem.org

(Difference between revisions)
 
(87 intermediate revisions not shown)
Line 28: Line 28:
             background:#a2dcf3;
             background:#a2dcf3;
             color: #5a5a5a;
             color: #5a5a5a;
-
             font-size: 12px;
+
             font-size: 14px;
-
        }
+
             font-family:Andalus;
-
        li{
+
-
             list-style-type:none;
+
         }
         }
         #myheader{
         #myheader{
Line 51: Line 49:
             padding-top: 100px;
             padding-top: 100px;
             margin-bottom: 20px;
             margin-bottom: 20px;
-
             font-family: Georgia, Times, "Times New Roman", serif;
+
             font-family: Georgia, Times, "Andalus", serif;
-
        }
+
-
 
+
-
        h1, h2, h3, h4, h5 {
+
-
            font-family: Georgia, Times, "Times New Roman", serif;
+
         }
         }
 +
        h1, h2, h3, h4, h5 {font-family: Georgia, Times, "Andalus", serif;}
         #top-section {
         #top-section {
-
             /*-- styling for default menu bar (edit, page, history, etc.) --*/
+
             /*-- styling for default menu bar (edit, page, history, etc.) --*/ background-color: #383838;border: 0 none;height: 14px;z-index: 100;top: 0;position: fixed; width: 975px;left: 50%;margin-left: -487px;}
-
            background-color: #383838;
+
#top-section-bar {
-
            border: 0 none;
+
-
            height: 14px;
+
-
            z-index: 100;
+
-
            top: 0;
+
-
            position: fixed;
+
-
            width: 975px;
+
-
            left: 50%;
+
-
            margin-left: -487px;
+
-
        }
+
-
 
+
-
        #top-section-bar {
+
             /*-- styling full width bar which hides behind default menu bar (edit, page, history, etc.) --*/
             /*-- styling full width bar which hides behind default menu bar (edit, page, history, etc.) --*/
-
            background-color: #383838;
+
  background-color: #383838;
-
            height: 14px;
+
  height: 14px;
-
            display: block;
+
  display: block;
-
            z-index: 10;
+
  z-index: 10;position: fixed; width: 100%; top: 0;}
-
            position: fixed;
+
  body { background-color: #fff;
-
            width: 100%;
+
  }
-
            top: 0;
+
#globalWrapper, #content {
-
        }
+
  /*-- changes default wiki settings --*/
 +
  width: 100%;
 +
  height: 100%;
 +
  border: 0px;
 +
  background-color: transparent;
 +
  margin: 0px;
 +
  padding: 0px;
 +
  }
-
        body {
+
  html, body, .wrapper {
-
            background-color: #fff;
+
  /*-- changes default wiki settings --*/
-
        }
+
  width: 100%;
-
 
+
  height: 100%;
-
        #globalWrapper, #content {
+
  background-color: transparent;
-
            /*-- changes default wiki settings --*/
+
  } #container {
-
            width: 100%;
+
  /*-- creates container for all content on page --*/
-
            height: 100%;
+
  font-family: Arial, Helvetica, sans-serif;
-
            border: 0px;
+
-
            background-color: transparent;
+
-
            margin: 0px;
+
-
            padding: 0px;
+
-
        }
+
-
 
+
-
        html, body, .wrapper {
+
-
            /*-- changes default wiki settings --*/
+
-
            width: 100%;
+
-
            height: 100%;
+
-
            background-color: transparent;
+
-
        }
+
-
 
+
-
        #container {
+
-
            /*-- creates container for all content on page --*/
+
-
            font-family: Arial, Helvetica, sans-serif;
+
             font-weight: normal;
             font-weight: normal;
             font-size: 14px;
             font-size: 14px;
Line 113: Line 88:
             margin-right: auto;
             margin-right: auto;
             background-color: #transparent;
             background-color: #transparent;
-
             margin-top: 0px;
+
             margin-top: 0px;}
-
         }
+
.container {
 +
            font-size: 14px;
 +
            width:700px;
 +
            float:right;}
 +
.buttontext { color: #FFF}
 +
#my_h1{position:absolute;top:70px;left:170px;border:none;}
 +
#my_h1 a{display:block;width:190px;height:130px;text-indent:-9999px;}
 +
#my_h2{position:absolute;top:120px;left:370px;border:none;}
 +
#my_h2 a{display:block;width:390px;height:230px;text-indent:-9999px;}
 +
#my_nav{position:absolute;top:402px;left:200px;width:990px;height:50px;}
 +
#my_nav ul{width:100%;margin:0;padding:0;list-style:none;font-size:24px;}
 +
#my_nav ul li{display::inline-block;padding:9px 30px;float:left;list-style:none;position:relative;}
 +
#my_nav ul li:hover{background:#e3f7ff;}#my_nav ul li a{color:#00586b;text-decoration:none;}#project{display:none;position:absolute;z-index:100;top:47px;left:0px;background:#CF6;width:100px;}#project dd{width:135px;font-size:16px;
 +
background:#c9efff;height:40px;margin:0;}#project dd a{width:100%;height:40px;display:block;text-decoration:none;text-align:center;line-height:40px;}#project dd a:hover{background:#a1dbf0;}#achievement{display:none;position:absolute;top:47px;z-index:100;left:0px;background:#CF6;width:100px;}#achievement dd{width:198px;font-size:16px;background:#c9efff;height:40px;margin:0;}#achievement dd a{width:100%;height:40px;display:block;text-decoration:none;text-align:center;line-height:40px;}#achievement dd a:hover{background:#a1dbf0;}#prctice{display:none;position:absolute;top:47px;z-index:100;left:0px;background:#CF6;width:100px;}#prctice dd{width:300px;font-size:16px;background:#c9efff;height:40px;margin:0;}#prctice dd a{width:100%;height:40px;display:block;text-decoration:none;text-align:center;line-height:40px;}#prctice dd a:hover{background:#a1dbf0;}#ackonwledgement{display:none;position:absolute;top:47px;z-index:100;left:0px;background:#CF6;width:100px;}#ackonwledgement dd{width:257px;font-size:16px;background:#c9efff;height:40px;margin:0;}#ackonwledgement dd a{width:100%;height:40px;display:block;text-decoration:none;text-align:center;line-height:40px;}#ackonwledgement dd a:hover{background:#a1dbf0;}
 +
#my_content{
 +
width:1030px;
 +
margin:0px auto;}
 +
#my_abstract{
 +
width:300px;
 +
margin:0 0 0 25px;
 +
         float:left;
 +
}
 +
#my_abstract h2{
 +
height:50px;
 +
line-height:50px;
 +
text-indent:50px;
 +
border:none;
 +
background:#999;
 +
margin:0 0 20px 0;
 +
padding:0;
 +
}
 +
#my_abstract p{
 +
text-indent:30px;
 +
height:240px;
 +
overflow-y:scroll;
 +
}
 +
 
-
        .container {
 
-
            font-size: 14px
 
-
        }
 
-
        .buttontext {
+
 
-
            color: #FFF
+
#my_contents{
-
        }
+
width:1030px;
-
#my_nav{
+
margin:30px auto;
-
position:absolute;
+
height:800px;
-
top:402px;
+
-
left:200px;
+
-
width:990px;
+
-
height:50px;
+
}
}
-
#my_nav ul{
+
#my_con{
-
width:100%;
+
width:1000px;
-
margin:0;
+
height:796px;
-
padding:0;
+
float:right;
-
list-style-type:none;
+
-
font-size:24px;
+
-
}
+
-
#my_nav ul li{
+
-
display::inline-block;
+
-
padding:9px 30px;
+
-
float:left;
+
position:relative;
position:relative;
 +
border:2px solid #999;
 +
        margin:20px 10px 0 0;
}
}
-
#my_nav ul li:hover{
+
#stage_1{
-
background:#e3f7ff;
+
width:20px;
 +
height:20px;
 +
position:absolute;
 +
top:40px;
 +
left:40px;
 +
border-left:2px solid #333;
 +
border-top:2px solid #333;
}
}
-
#my_nav ul li a{
+
#stage_2{
-
color:#00586b;
+
width:20px;
-
text-decoration:none;
+
height:20px;
 +
position:absolute;
 +
top:40px;
 +
left:940px;
 +
border-right:2px solid #333;
 +
border-top:2px solid #333;
}
}
-
#project{
+
#stage_3{
-
display:none;
+
width:20px;
 +
height:20px;
position:absolute;
position:absolute;
-
        index:10;
+
top:740px;
-
top:47px;
+
left:40px;
-
left:0px;
+
border-bottom:2px solid #333;
-
background:#CF6;
+
border-left:2px solid #333;
-
width:100px;
+
}
}
-
#project dd{
+
#stage_4{
-
width:135px;
+
width:20px;
-
font-size:16px;
+
height:20px;
-
background:#c9efff;
+
position:absolute;
-
height:40px;
+
top:740px;
-
margin:0;
+
left:940px;
 +
border-right:2px solid #333;
 +
border-bottom:2px solid #333;
}
}
-
#project dd a{
+
#real_con{
-
width:100%;
+
-
height:40px;
+
-
display:block;
+
-
text-decoration:none;
+
-
text-align:center;
+
-
line-height:40px;
+
-
}
+
-
#project dd a:hover{
+
-
background:#a1dbf0;
+
-
}
+
-
#achievement{
+
-
display:none;
+
position:absolute;
position:absolute;
-
top:47px;
+
top:60px;
-
        index:10;
+
left:60px;
-
left:0px;
+
width:880px;
-
background:#CF6;
+
height:680px;
-
width:100px;
+
}
}
-
#achievement dd{
+
#real_con ul{
-
width:198px;
+
list-style:none;
-
font-size:16px;
+
width:100%;
-
background:#c9efff;
+
height:100%;
-
height:40px;
+
margin:0;
margin:0;
 +
padding:0;
}
}
-
#achievement dd a{
+
#real_con ul li{
-
width:100%;
+
width:400px;
-
height:40px;
+
height:300px;
-
display:block;
+
float:left;
 +
background:#0F0;
 +
margin:26px 0 0 26px;}
 +
#real_con ul li#one_1{
 +
background:url("https://static.igem.org/mediawiki/2014/b/b4/Project_li_bg.png") no-repeat;}
 +
#real_con ul li#two_2{
 +
background:url("https://static.igem.org/mediawiki/2014/1/17/Model_li_bg.png") no-repeat;}
 +
#real_con ul li#three_3{
 +
background:url("https://static.igem.org/mediawiki/2014/6/6e/Weblab_li_bg.png") no-repeat;}
 +
#real_con ul li#four_4{
 +
background:url("https://static.igem.org/mediawiki/2014/f/f0/Practice_li_bg.png") no-repeat;}
 +
#real_con ul li a{
text-decoration:none;
text-decoration:none;
-
text-align:center;
 
-
line-height:40px;
 
-
}
 
-
#achievement dd a:hover{
 
-
background:#a1dbf0;
 
-
}
 
-
#prctice{
 
-
display:none;
 
-
position:absolute;
 
-
top:47px;
 
-
        index:10;
 
-
left:0px;
 
-
background:#CF6;
 
-
width:100px;
 
-
}
 
-
#prctice dd{
 
-
width:300px;
 
-
font-size:16px;
 
-
background:#c9efff;
 
-
height:40px;
 
-
margin:0;
 
-
}
 
-
#prctice dd a{
 
-
width:100%;
 
-
height:40px;
 
display:block;
display:block;
-
text-decoration:none;
 
-
text-align:center;
 
-
line-height:40px;
 
-
}
 
-
#prctice dd a:hover{
 
-
background:#a1dbf0;
 
-
}
 
-
#ackonwledgement{
 
-
display:none;
 
-
position:absolute;
 
-
top:47px;
 
-
        index:10;
 
-
left:0px;
 
-
background:#CF6;
 
-
width:100px;
 
-
}
 
-
#ackonwledgement dd{
 
-
width:257px;
 
-
font-size:16px;
 
-
background:#c9efff;
 
-
height:40px;
 
-
margin:0;
 
-
}
 
-
#ackonwledgement dd a{
 
width:100%;
width:100%;
-
height:40px;
+
height:100%;
-
display:block;
+
-
text-decoration:none;
+
-
text-align:center;
+
-
line-height:40px;
+
-
}
+
-
#ackonwledgement dd a:hover{
+
-
background:#a1dbf0;
+
}
}
 +
#real_con ul li a#one:hover{
 +
background:url("https://static.igem.org/mediawiki/2014/6/68/Project_a_bg.png") no-repeat;}
 +
 +
#real_con ul li a#two:hover{
 +
background:url("https://static.igem.org/mediawiki/2014/d/dc/Model_a_bg.png") no-repeat;}
 +
#real_con ul li a#three:hover{
 +
background:url("https://static.igem.org/mediawiki/2014/6/60/Weblab_a_bg.png") no-repeat;}
 +
#real_con ul li a#four:hover{
 +
background:url("https://static.igem.org/mediawiki/2014/7/7f/Practice_a_bg.png") no-repeat;}
 +
 +
 +
#thanks{
 +
height:177px;
 +
width:1030px;
 +
padding:30px 0 0 0;
 +
        clear:both;
 +
margin:30px auto 0;}
 +
 +
     </style>
     </style>
     <style>
     <style>
Line 299: Line 272:
<body>
<body>
<div id="myheader">
<div id="myheader">
-
<h1><a href="#"></a></h1>
+
<h1 id="my_h1"><a href="https://2014.igem.org">https://2014.igem.org</a></h1>
 +
<h2 id="my_h2"><a href="https://igem.org/Team.cgi?id=1555">https://igem.org/Team.cgi?id=1555</a></h2>
     <div id="my_nav">
     <div id="my_nav">
         <ul>
         <ul>
-
           <li><a href="#">Home</a></li>
+
           <li><a href="https://2014.igem.org/Team:NJAU_China">Home</a></li>
           <li id="project_li"><a href="#">Project</a>
           <li id="project_li"><a href="#">Project</a>
                 <dl id="project">
                 <dl id="project">
-
                     <dd><a href="#">Background</a></dd>
+
                     <dd><a href="https://2014.igem.org/Team:NJAU_China/Background">Background</a></dd>
-
                     <dd><a href="#">System Design</a></dd>
+
                     <dd><a href="https://2014.igem.org/Team:NJAU_China/System Design">System Design</a></dd>
-
                     <dd><a href="#">Safety</a></dd>
+
                     <dd><a href="https://2014.igem.org/Team:NJAU_China/Safety">Safety</a></dd>
                 </dl>
                 </dl>
           </li>
           </li>
           <li id="achievement_li"><a href="#">Achievement</a>
           <li id="achievement_li"><a href="#">Achievement</a>
                 <dl id="achievement">
                 <dl id="achievement">
-
                     <dd><a href="#">Wet Labs</a></dd>
+
                     <dd><a href="https://2014.igem.org/Team:NJAU_China/Wet Labs">Wet Lab</a></dd>
-
                     <dd><a href="#">Modeling</a></dd>
+
                     <dd><a href="https://2014.igem.org/Team:NJAU_China/Modeling">Model</a></dd>
-
                     <dd><a href="#">Protocol/Notebooks</a></dd>
+
                     <dd><a href="https://2014.igem.org/Team:NJAU_China/Protocol/Notebooks">Protocol/Notebooks</a></dd>
-
                     <dd><a href="#">Biobricks</a></dd>
+
                     <dd><a href="https://2014.igem.org/Team:NJAU_China/Biobricks">Biobricks</a></dd>
                 </dl>
                 </dl>
           </li>
           </li>
-
           <li id="prctice_li"><a href="#">Prctice</a>
+
           <li id="prctice_li"><a href="#">Practice</a>
                 <dl id="prctice">
                 <dl id="prctice">
-
                     <dd><a href="#">iGEM and Synthetic Biology Exhibition</a></dd>
+
                     <dd><a href="https://2014.igem.org/Team:NJAU_China/Exhibition and Workshops">Exhibitions and Workshops</a></dd>
-
                     <dd><a href="#">Questionnaire Survey</a></dd>
+
                     <dd><a href="https://2014.igem.org/Team:NJAU_China/Questionnaire Survey">Questionnaire Survey</a></dd>
-
                     <dd><a href="#">Collaboration</a></dd>
+
                     <dd><a href="https://2014.igem.org/Team:NJAU_China/Collaboration">Collaboration</a></dd>
                 </dl>
                 </dl>
           </li>
           </li>
-
           <li><a href="#">Team</a></li>
+
           <li><a href="https://2014.igem.org/Team:NJAU_China/Team">Team</a></li>
-
           <li id="ackonwledgement_li"><a href="#">Ackonwledgement</a>
+
           <li id="ackonwledgement_li"><a href="#">Acknowledgement</a>
                 <dl id="ackonwledgement">
                 <dl id="ackonwledgement">
-
                    <dd><a href="#">Genewiz Corporation</a></dd>
+
                      <dd><a href="https://2014.igem.org/Team:NJAU_China/Inside Campus">Inside Campus&Arrtibutions</a></dd>
-
                     <dd><a href="#">Chinese Academy of Sciences</a></dd>
+
                     <dd><a href="https://2014.igem.org/Team:NJAU_China/Outside Campus">Outside Campus</a></dd>
                 </dl>
                 </dl>
           </li>
           </li>
       </ul>
       </ul>
-
</div>
+
    </div>
</div>
</div>
-
<div class="container-fluid">
 
-
    <!-- Brand and toggle get grouped for better mobile display -->
 
-
    <div class="navbar-header">
 
-
        <button type="button" class="navbar-toggle collapsed" data-toggle="collapse"
 
-
                data-target="#bs-example-navbar-collapse-1"><span class="sr-only">Toggle navigation</span> <span
 
-
                class="icon-bar"></span> <span class="icon-bar"></span> <span class="icon-bar"></span></button>
 
-
        <a class="navbar-brand" href="#">Brand</a></div>
 
-
    <div class="navbar-wrapper">
 
-
        <div class="navbar navbar-fixed-top navbar-inverse" role="navigation">
 
-
            <div class="container">
 
-
                <div class="navbar-header">
 
-
                    <button type="button" class="navbar-toggle collapsed" data-toggle="collapse"
 
-
                            data-target=".navbar-collapse"><span class="sr-only">Toggle navigation</span> <span
 
-
                            class="icon-bar"></span> <span class="icon-bar"></span> <span class="icon-bar"></span>
 
-
                    </button>
 
-
                    <a href="https://2014.igem.org/Main_Page" class="navbar-brand">
 
-
                        <img src="https://static.igem.org/mediawiki/2014/2/22/Logo2014v2.png" height="30" width="80">
 
-
                    </a>
 
-
                    <a class="navbar-brand" href="https://2014.igem.org/Team:NJAU_China/">NJAU_China</a></div>
 
-
                <div class="collapse navbar-collapse">
 
-
                    <ul class="nav navbar-nav">
 
-
                        <li class="active"><a href="https://2014.igem.org/Team:NJAU_China" class="unselect">Home</a></li>
 
-
                        <li class="dropdown"><a href="#" class="dropdown-toggle" data-toggle="dropdown">Project<span
 
-
                                class="caret"></span></a>
 
-
                            <ul class="dropdown-menu" role="menu">
 
-
                                <li><a href="https://2014.igem.org/Team:NJAU_China/Background">Background</a></li>
 
-
                                <li><a href="https://2014.igem.org/Team:NJAU_China/System Design">System Design</a></li>
 
-
                                <li><a href="https://2014.igem.org/Team:NJAU_China/Safety">Safety</a></li>
 
-
                            </ul>
 
-
                        </li>
 
-
                        <li class="dropdown"><a href="#" class="dropdown-toggle" data-toggle="dropdown">Achievement<span
 
-
                                class="caret"></span></a>
 
-
                            <ul class="dropdown-menu" role="menu">
 
-
                                <li><a href="https://2014.igem.org/Team:NJAU_China/Modeling">Modeling</a></li>
 
-
                                <li><a href="https://2014.igem.org/Team:NJAU_China/Biobricks">Biobricks</a></li>
 
-
                                <li><a href="https://2014.igem.org/Team:NJAU_China/Judging">Judging</a></li>
 
-
                            </ul>
 
-
                        </li>
 
-
                        <li><a href="https://2014.igem.org/Team:NJAU_China/Human Practice" class="unselect">Human Practice</a></li>
 
-
                        <li><a href="#" class="dropdown-toggle" data-toggle="dropdown">Wet Lab<span
 
-
                                class="caret"></span></a>
 
-
                            <ul class="dropdown-menu" role="menu">
 
-
                                <li><a href="https://2014.igem.org/Team:NJAU_China/Biobricks">Biobricks</a></li>
 
-
                            </ul>
 
-
                        </li>
 
-
                        <li><a href="https://2014.igem.org/Team:NJAU_China/Team" class="unselect">Team</a></li>
 
-
                        <li><a href="https://2014.igem.org/Team:NJAU_China/Acknowledgement" class="unselect">Acknowledgement</a>
 
-
                        </li>
 
-
                    </ul>
 
-
                </div>
 
-
                <!-- /.nav-collapse -->
 
-
            </div>
 
-
            <!-- /.container -->
 
-
        </div>
 
-
        <!-- /.navbar -->
 
-
    </div>
+
<!---------导航结束------------>
-
</div>
+
 
<!-- Carousel
<!-- Carousel
     ================================================== -->
     ================================================== -->
-
 
+
<div id="my_content">
 +
<div id="my_abstract">
 +
    <h2>Abstract</h2>
 +
    <p>As an common pollutant source of industry, low concentration of copper ions can do harm to many creatures. Whereas, <i>Escherichia coli</i> has high tolerance for copper through specific systems. There are <i>cop</i>A promoter,<i>mar</i>O(operator of mar locus) and <i>cue</i>O promoter existing in <i>E.Coli</i>, all of which are directly or indirectly regulated by copper ions. The most sensitive and effective one (<i>cop</i>A)was chosen to trigger the sequential reactions. Glutathione is an intracellular redox-active tripeptide thiol which can effectively detoxify the metal ions by chelation. It can be synthesized by <i>gsh</i>F, an coding sequence derived from <i>Streptococcus thermophilus</i>, which can unify both necessary reactions in one platform.In addition, we also utilize sgRNA-dCas9 system to enhance the efficiency of chelate cupric and cuprous by repressing the expression of <i>cop</i>A. With all these design, an automatically and efficiently copper-preventing guard have been created successfully. </p>
 +
</div>
<div class="container">
<div class="container">
-
 
     <div id="myCarousel" class="carousel slide" data-ride="carousel">
     <div id="myCarousel" class="carousel slide" data-ride="carousel">
         <!-- Indicators -->
         <!-- Indicators -->
Line 410: Line 332:
         <div class="carousel-inner" role="listbox">
         <div class="carousel-inner" role="listbox">
             <div class="item active text-center"><img src="https://static.igem.org/mediawiki/2014/d/d3/Njau_slide1.jpg"
             <div class="item active text-center"><img src="https://static.igem.org/mediawiki/2014/d/d3/Njau_slide1.jpg"
-
                                                       alt="First slide" class="text-center">
+
                                                       alt="First slide" class="text-center"></div>
-
 
+
             <div class="item text-center"><img src="https://static.igem.org/mediawiki/2014/3/38/Face.jpg"
-
                <div class="container">
+
                                               alt="Second slide"></div>
-
                    <div class="carousel-caption">
+
             <div class="item text-center"><img src="https://static.igem.org/mediawiki/2014/9/94/Face2.jpg"
-
                        <h1>The First Teaching Building</h1>
+
                                               alt="Third slide"></div>
-
 
+
-
                        <p>Photoed by TianYC.</p>
+
-
 
+
-
                        <p><a class="btn btn-lg btn-primary" style="color:#FFF" href="http://english.njau.edu.cn/"
+
-
                              role="button">Learn more</a></p>
+
-
                    </div>
+
-
                </div>
+
-
            </div>
+
-
             <div class="item text-center"><img src="https://static.igem.org/mediawiki/2014/2/24/Njau_slide2.jpg"
+
-
                                               alt="Second slide">
+
-
 
+
-
                <div class="container">
+
-
                    <div class="carousel-caption">
+
-
                        <h1>Quadripod Ding</h1>
+
-
 
+
-
                        <p>A landmark of Nanjing Agricultural University, which means NJAU was one branch of National
+
-
                            Central University</p>
+
-
 
+
-
                        <p><a class="btn btn-lg btn-primary" style="color:#FFF"
+
-
                              href="http://english.njau.edu.cn/html/About/History/1.html" role="button">Learn more</a>
+
-
                        </p>
+
-
                    </div>
+
-
                </div>
+
-
            </div>
+
-
             <div class="item text-center"><img src="https://static.igem.org/mediawiki/2014/6/62/Njau_slide3.jpg"
+
-
                                               alt="Third slide">
+
-
 
+
-
                <div class="container">
+
-
                    <div class="carousel-caption">
+
-
                        <h1>Photo of team members</h1>
+
-
 
+
-
                        <p>Exclude two people who are abroad</p>
+
-
 
+
-
                        <p><a class="btn btn-lg btn-primary" style="color:#FFF" href="http://lfc.njau.edu.cn:8026/"
+
-
                              role="button">Learn more</a></p>
+
-
                    </div>
+
-
                </div>
+
-
            </div>
+
         </div>
         </div>
         <a class="left carousel-control" href="#myCarousel" role="button" data-slide="prev"> <span class="sr-only">Previous</span>
         <a class="left carousel-control" href="#myCarousel" role="button" data-slide="prev"> <span class="sr-only">Previous</span>
Line 459: Line 343:
     <!-- /.carousel -->
     <!-- /.carousel -->
</div>
</div>
-
<!-- Marketing messaging and featurettes
+
</div>
-
    ================================================== -->
+
<div id="my_contents">
-
<!-- Wrap the rest of the page in another container to center all the content. -->
+
<div id="my_con">
-
 
+
    <div id="stage_1"></div>
-
<div class="container marketing">
+
        <div id="stage_2"></div>
-
 
+
        <div id="stage_3"></div>
-
    <!-- START THE FEATURETTES -->
+
         <div id="stage_4"></div>
-
 
+
        <div id="real_con">
-
    <hr class="featurette-divider">
+
        <ul>
-
    <div class="row featurette">
+
            <li id="one_1"><a href="https://2014.igem.org/Team:NJAU_China/Background" id="one"></a></li>
-
         <div class="col-md-7">
+
                <li id="two_2"><a href="https://2014.igem.org/Team:NJAU_China/Modeling" id="two"></a></li>
-
            <h2 class="featurette-heading">First featurette heading. <span
+
                 <li id="three_3"><a href="https://2014.igem.org/Team:NJAU_China/Wet_Labs" id="three"></a></li>
-
                    class="text-muted">It'll blow your mind.</span></h2>
+
                 <li id="four_4"><a href="https://2014.igem.org/Team:NJAU_China/Questionnaire_Survey" id="four"></a></li>
-
 
+
            </ul>
-
            <p class="lead">Donec ullamcorper nulla non metus auctor fringilla. Vestibulum id ligula porta felis euismod
+
-
                 semper. Praesent commodo cursus magna, vel scelerisque nisl consectetur. Fusce dapibus, tellus ac cursus
+
-
                 commodo.</p>
+
         </div>
         </div>
-
        <div class="col-md-5"><img src="https://static.igem.org/mediawiki/2014/1/10/Njau_home_1.jpg"
 
-
                                  alt="Generic placeholder image" class="featurette-image img-responsive"></div>
 
     </div>
     </div>
-
    <hr class="featurette-divider">
+
</div>
-
    <div class="row featurette">
+
-
        <div class="col-md-5"><img src="https://static.igem.org/mediawiki/2014/8/8a/Njau_home_2.jpg"
+
-
                                  alt="Generic placeholder image" class="featurette-image img-responsive"></div>
+
-
        <div class="col-md-7">
+
-
            <h2 class="featurette-heading">Oh yeah, it's that good. <span class="text-muted">See for yourself.</span>
+
-
            </h2>
+
-
            <p class="lead">Donec ullamcorper nulla non metus auctor fringilla. Vestibulum id ligula porta felis euismod
+
<div id="thanks">
-
                semper. Praesent commodo cursus magna, vel scelerisque nisl consectetur. Fusce dapibus, tellus ac cursus
+
<img src="https://static.igem.org/mediawiki/2014/2/26/Njau_bg.png" alt="njau"><img src="https://static.igem.org/mediawiki/2014/a/ae/Shengke_bg.png" alt="shengke">
-
                commodo.</p>
+
-
        </div>
+
-
    </div>
+
-
    <hr class="featurette-divider">
+
-
    <div class="row featurette">
+
-
        <div class="col-md-7">
+
-
            <h2 class="featurette-heading">And lastly, this one. <span class="text-muted">Checkmate.</span></h2>
+
-
 
+
-
            <p class="lead">Donec ullamcorper nulla non metus auctor fringilla. Vestibulum id ligula porta felis euismod
+
-
                semper. Praesent commodo cursus magna, vel scelerisque nisl consectetur. Fusce dapibus, tellus ac cursus
+
-
                commodo.</p>
+
-
        </div>
+
-
        <div class="col-md-5"><img src="https://static.igem.org/mediawiki/2014/1/1e/Njau_home_3.jpg"
+
-
                                  alt="Generic placeholder image" class="featurette-image img-responsive"></div>
+
-
    </div>
+
-
    <hr class="featurette-divider">
+
-
 
+
-
    <!-- /END THE FEATURETTES -->
+
-
 
+
-
    <!-- FOOTER -->
+
-
    <footer>
+
-
        <p class="pull-right"><a href="#">Back to top</a></p>
+
-
 
+
-
        <p>&copy;  NJAU@2014 iGEM. &middot;</p>
+
-
    </footer>
+
</div>
</div>
-
<!-- /.container -->
 
-
 
-
<!-- Bootstrap core JavaScript
 
-
    ================================================== -->
 
-
<!-- Placed at the end of the document so the pages load faster -->
 
-
 
</body>
</body>
</html>
</html>

Latest revision as of 17:03, 19 November 2014

Home

Abstract

As an common pollutant source of industry, low concentration of copper ions can do harm to many creatures. Whereas, Escherichia coli has high tolerance for copper through specific systems. There are copA promoter,marO(operator of mar locus) and cueO promoter existing in E.Coli, all of which are directly or indirectly regulated by copper ions. The most sensitive and effective one (copA)was chosen to trigger the sequential reactions. Glutathione is an intracellular redox-active tripeptide thiol which can effectively detoxify the metal ions by chelation. It can be synthesized by gshF, an coding sequence derived from Streptococcus thermophilus, which can unify both necessary reactions in one platform.In addition, we also utilize sgRNA-dCas9 system to enhance the efficiency of chelate cupric and cuprous by repressing the expression of copA. With all these design, an automatically and efficiently copper-preventing guard have been created successfully.

njaushengke