Team:WashU StLouis/Attributions
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- | < | + | <div style="text-align: center;"> |
- | + | <h3>iGEM team members</h3> | |
- | < | + | </div> |
- | + | <br> | |
- | </ | + | <div style="margin-left: 40px;">The Brauer Group: Jeffrey worked with |
- | + | Benjamin Huang as part of the Brauer Group. They were in charge of the | |
- | + | light regulation aspect of the WashU iGEM project. Ben and Jeffrey | |
- | + | spent most of the summer cloning and testing the light regulated | |
- | < | + | transcription elements. <br> |
- | < | + | <br> |
- | < | + | <div style="margin-left: 40px;">Ben also spent a significant amount of |
- | < | + | time coding and making the team wiki page look pretty awesome. He did |
- | + | most of the coding for the site through self-learning of html and often | |
- | + | sought Google for help involving web design elements. He did not use | |
- | < | + | any outside resources or companies to code the site, but borrowed |
- | + | inspiration and snippets of code from previous iGEM teams: in | |
- | < | + | particular 2013 WLC-Milwaukee, 2013 TMU-Tokyo, and 2012 WashU. He also |
- | + | began work on the kinetic model system of the light regulation system. | |
- | < | + | He continued cloning work during the school year, along with taking over organization of the team and keeping up with the many deadlines when Jeffrey went |
- | + | abroad.<br> | |
- | < | + | <br> |
- | + | Jeffrey worked on managing the team, setting up meetings, and having | |
- | < | + | conversations with the school administration and other iGEM teams. He |
- | + | also worked to create our team apparel.<br> | |
- | < | + | </div> |
- | + | <br> | |
- | < | + | The Rebstock Group: Caroline Focht worked with Richard Li as part of |
- | + | the Rebstock Group. They were in charge of the plasmid design and Nif | |
- | < | + | activity testing aspect of the WashU iGEM project. Caroline and Richard |
- | + | spent most of their time in summer in protocol development and nitrogen | |
- | + | fixation activity testing. They continued running experiments and | |
- | < | + | biobricked during the school year.<br> |
- | < | + | <br> |
- | < | + | <div style="margin-left: 40px;">Caroline also concentrated on WashU |
- | + | iGEM Outreach program, which produced numerous educational videos, and | |
- | < | + | sought out other iGEM teams for collaborations. She also produced most |
- | + | of our logos and graphics for the website and presentations.<br> | |
- | < | + | <br> |
- | < | + | Richard was lab manger of iGEM at Washington University in St. Louis, |
- | + | thus in addition to the wet lab work he was also in charge of | |
- | < | + | fundraising and securing for the project. <br> |
- | + | </div> | |
- | < | + | </div> |
- | + | <br> | |
- | < | + | <div style="text-align: center;"> |
- | + | <h3>Attributions:</h3> | |
- | < | + | </div> |
- | + | <span style="text-decoration: underline; font-style: italic;">Project | |
- | < | + | Support:</span><br> |
- | + | <br> | |
- | < | + | <div style="margin-left: 40px;">Dr. Himadri Pakrasi and Dr. Tae Seok |
- | + | Moon served as mentors during our iGEM project. We had a few meetings | |
- | < | + | with them in order to present our project proposals and check on our |
- | + | progress updates, and helped to give us feedback and troubleshoot when | |
- | < | + | necessary. They provided the funding for lab supplies.<br> |
- | + | <br> | |
- | < | + | <div style="margin-left: 40px;">Dr. Pakrasi: Dr. Pakrasi is Myron and |
- | + | Sonya Glassberg/Albert and Blanche Greensfelder Distinguished | |
- | </ | + | University Professor, also Director of International Center for |
+ | Advanced Renewable Energy and Sustainability (I-CARES). His research | ||
+ | interests are: Photosynthesis, Nitrogen Fixation, Systems and Synthetic | ||
+ | Biology. Pakrasi Lab’s current focus is on Bioenergy Production in | ||
+ | Cyanobacteria.<br> | ||
+ | <br> | ||
+ | Dr. Tae Seok Moon: Dr. Moon is a Principal Investigator and Assistant | ||
+ | Professor in the Department of Energy, Environmental & Chemical | ||
+ | Engineering (EECE) at Washington University in St. Louis and heads the | ||
+ | Moon Research Group (MRG). He generously allowed Ben and Jeffrey | ||
+ | unfettered access to his lab and material equipment at all hours of the | ||
+ | day (and night). Dr. Moon also financed ordering of primers and DNA | ||
+ | sequence verification.<br> | ||
+ | </div> | ||
+ | <br> | ||
+ | Our team would not be complete without our list of direct advisors: | ||
+ | Andrew Ng, Bert Berla, Cheryl Immethun, and Dr. Deng Liu.<br> | ||
+ | <br> | ||
+ | <div style="margin-left: 40px;">Andrew Ng: Andrew is an | ||
+ | undergraduate biomedical engineer interested in the applications of | ||
+ | systems and synthetic biology to cyanobacteria. He was also a past | ||
+ | participant in iGEM at WashU. As the major peer advisor for iGEM | ||
+ | project this year, Andrew provided invaluable advice and guidance on | ||
+ | all aspects of both Rebstock and Brauer Group’s work, ranging from | ||
+ | protocol design and lab technique training to providing material | ||
+ | support for the GC activity testing. <br> | ||
+ | <br> | ||
+ | Bertram Berla: Bert is a PhD candidate in Energy, Environmental, | ||
+ | and Chemical Engineering. He is working to apply techniques developed | ||
+ | to optimize the operation of chemical reactors to get more useful stuff | ||
+ | out of our favorite micro-reactors: cyanobacteria. Bert assisted | ||
+ | Richard and Caroline on experimental design. <br> | ||
+ | <br> | ||
+ | Cheryl Immethun: Ms. Immethun is currently a PhD candidate in the MRG | ||
+ | with Dr. Moon in the EECE department at Washington University in St. | ||
+ | Louis. She serves as a senior iGEM advisor and over the course of the | ||
+ | summer, provided invaluable advice and guidance on all aspects of the | ||
+ | Brauer Group’s work, ranging from primer design and DNA cloning issues | ||
+ | to providing material support for the light experiment. She also aided | ||
+ | with cloning during the school year closer to the deadline as work | ||
+ | conflicts slowed down progress.<br> | ||
+ | <br> | ||
+ | Deng Liu: Deng is interested in the research on cyanobacteria, | ||
+ | especially on the synthetic biology and transcriptional regulation in | ||
+ | cyanobacteria. Deng helped iGEM members on details of experimental | ||
+ | design, plasmid design, and cell culturing.<br> | ||
+ | </div> | ||
+ | <br> | ||
+ | <br> | ||
+ | Our Non-iGEM affiliated advisors worked in their respective labs and | ||
+ | would help us on a daily basis, whether it giving valuable input on | ||
+ | experimental or cloning protocols or feedback during our project | ||
+ | presentations. The list includes:<br> | ||
+ | <br> | ||
+ | <div style="margin-left: 40px;">Allie Hoynes-O’Connor, Ray Henson, Ryan | ||
+ | Lee , and Tatenda Shopera: The graduate students of the MRG helped the | ||
+ | Brauer Group by aiding in answering every day questions and making sure | ||
+ | we contributed to the well-being and material readiness of the lab as | ||
+ | well as following all safety and labeling protocols. Ms. | ||
+ | Hoynes-O’Connor served as our lab safety officer and gave us guidance | ||
+ | and training on lab safety procedures. Mr. Henson also gave guidance | ||
+ | and plasmid material in the Brauer Group’s early attempts at | ||
+ | constructing a sequence verifiable chromophore. <br> | ||
+ | <br> | ||
+ | Kenneth Ng and Soo Ji Kim: Kenneth and Soo Ji are laboratory technician | ||
+ | staff in the MRG and helped resupply the lab’s vital operating stock | ||
+ | materials and equipment in addition to giving advice and answering | ||
+ | questions the iGEM students had. Kenneth provided his advanced | ||
+ | expertise in adjusting and optimizing protocols drawn from his vast | ||
+ | knowledge due to a highly inquisitive nature. <br> | ||
+ | <br> | ||
+ | Nancy Duan: Nancy is the technician in the Rebstock lab. She takes | ||
+ | responsibilities of maintaining cyanobacteria culture and assisting | ||
+ | iGEM in doing experiments such as PCR, sub-cloning, plasmid preparation | ||
+ | etc. <br> | ||
+ | <br> | ||
+ | Jingjie Yu: Jingjie works on developing a new cyanobacteria model | ||
+ | system for synthetic biology purpose, especially, for biofuel | ||
+ | production. During iGEM project, she provided detailed advice on nearly | ||
+ | all aspect of experiment for Rebstock group.<br> | ||
+ | </div> | ||
+ | <br> | ||
+ | At Monsanto, a group of scientists specializing in nitrogen fixation | ||
+ | sat in on our team’s presentation about the outlook of our nitrogen | ||
+ | fixation project and offered plenty of suggestions through the advisory | ||
+ | panel meeting. The diagnostic panel meeting with scientists of Monsanto | ||
+ | later proved to be productive for the design and procedure of the lab | ||
+ | work.<br> | ||
+ | </div> | ||
+ | <br> | ||
+ | <span style="font-style: italic; text-decoration: underline;">Modeling | ||
+ | Support:</span><br> | ||
+ | <br> | ||
+ | <div style="margin-left: 40px;">Andrew Ng: Andrew also helped to create | ||
+ | the list of kinetic equations for our model. <br> | ||
+ | <br> | ||
+ | Brandon Nicklas: Brandon Nicklas is an undergraduate at Penn State; he | ||
+ | helped us to design a kinetic model for our light regulation system. Unfortunately we did not continue with the model as we were missing some experimental values that we ran out of time to find. <br> | ||
+ | <br> | ||
+ | Rajib Saha: Rajib is a PhD candidate at Penn State; he worked as a | ||
+ | liaison between Penn State and WashU and guided Brandon and gave us | ||
+ | feedback on the modeling.<br> | ||
+ | </div> | ||
+ | <br> | ||
+ | <span style="text-decoration: underline; font-style: italic;">Human | ||
+ | Practices Support:</span><br> | ||
+ | <br> | ||
+ | <div style="margin-left: 40px;">Beyond the support of lab work design, | ||
+ | Monsanto, and in particular Jose Prado, project lead of a paper | ||
+ | published about how projects go from concept to market, provided human | ||
+ | resources and materials including data sets, Power-point slides and | ||
+ | presentations to enhance WashU’s iGEM video series.<br> | ||
+ | </div> | ||
+ | <br> | ||
+ | <span style="text-decoration: underline; font-style: italic;">Financial | ||
+ | & Lab Facilities:</span><br> | ||
+ | <br> | ||
+ | <div style="margin-left: 40px;">Pakrasi Group: Dr. Pakrasi and his | ||
+ | research group at Washington University in St. Louis provided material | ||
+ | and working space support for both Rebstock and Brauer Group. <br> | ||
+ | <br> | ||
+ | Moon Group: Dr. Moon and his research group provided material and | ||
+ | working space support for the Brauer group.<br> | ||
+ | <br> | ||
+ | Zhang Group: Dr. Zhang and his research group at Washington University | ||
+ | in St. Louis provided material support when we were lacking certain | ||
+ | chemicals and let us use their temperature controlled growth chamber in | ||
+ | which we conducted our light regulation experiments. <br> | ||
+ | <br> | ||
+ | Sigma-Aldrich has supported WashU iGEM program since 2012. This year, | ||
+ | Tonya Jackson, the Product Manager of Genomics and Research, provided | ||
+ | help by not only offering funding for our project, gave us feedback on | ||
+ | our presentation, and also provided us a sample of a new prototype | ||
+ | media “EnPresso B” to test and enhance growth of our colonies.<br> | ||
+ | <br> | ||
+ | Monsanto’s sponsorship continued in 2014 through material and | ||
+ | advisorial means. On Wednesday, June 4, our team was invited to visit | ||
+ | Monsanto Headquarters and its affiliated facilities. The tour gave team | ||
+ | members a terrific impression about industrial-sized biotechnology | ||
+ | development procedure and growth capabilities.<br> | ||
+ | <br> | ||
+ | WashU iGEM would like to thank <a href="http://artsci.wustl.edu">Washington | ||
+ | University School of Arts & Sciences</a>, <a | ||
+ | href="http://engineering.wustl.edu">Washington University School of | ||
+ | Engineering</a>, <a href="http://undergradresearch.wustl.edu">Washington | ||
+ | University Office of Undergraduate Research</a>, <a | ||
+ | href="http://icares.wustl.edu/Pages/Home.aspx">Washington University | ||
+ | International Center for Advanced Renewable Energy and Sustainability</a>, | ||
+ | <a href="http://www.sigmaaldrich.com/united-states.html">Sigma-Aldrich</a> | ||
+ | and <a href="http://www.monsanto.com/pages/default.aspx">Monsanto</a> | ||
+ | for providing funding for stipends and materials. Lastly, we would like | ||
+ | to thank <a href="http://www.snapgene.com">SnapGene</a> for their | ||
+ | software to aid our plasmid designs. <br> | ||
+ | </div> | ||
+ | <br> | ||
+ | </body> | ||
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Latest revision as of 03:17, 18 October 2014
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