Team:NJAU China

From 2014.igem.org

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================================================== -->
================================================== -->
<body>
<body>
-
<div id="myheader"></div>
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<div id="myheader">
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<div class="container-fluid">
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<h1 id="my_h1"><a href="https://2014.igem.org">https://2014.igem.org</a></h1>
-
    <!-- Brand and toggle get grouped for better mobile display -->
+
<h2 id="my_h2"><a href="https://igem.org/Team.cgi?id=1555">https://igem.org/Team.cgi?id=1555</a></h2>
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         <ul>
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                data-target="#bs-example-navbar-collapse-1"><span class="sr-only">Toggle navigation</span> <span
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          <li><a href="https://2014.igem.org/Team:NJAU_China">Home</a></li>
-
                class="icon-bar"></span> <span class="icon-bar"></span> <span class="icon-bar"></span></button>
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          <li id="project_li"><a href="#">Project</a>
-
        <a class="navbar-brand" href="#">Brand</a></div>
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                <dl id="project">
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     <div class="navbar-wrapper">
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                     <dd><a href="https://2014.igem.org/Team:NJAU_China/Background">Background</a></dd>
-
         <div class="navbar navbar-fixed-top navbar-inverse" role="navigation">
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                     <dd><a href="https://2014.igem.org/Team:NJAU_China/System Design">System Design</a></dd>
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            <div class="container">
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                     <dd><a href="https://2014.igem.org/Team:NJAU_China/Safety">Safety</a></dd>
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                </dl>
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          </li>
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                            data-target=".navbar-collapse"><span class="sr-only">Toggle navigation</span> <span
+
          <li id="achievement_li"><a href="#">Achievement</a>
-
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                <dl id="achievement">
-
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                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Wet Labs">Wet Lab</a></dd>
-
                    <a href="https://2014.igem.org/Main_Page" class="navbar-brand">
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                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Modeling">Model</a></dd>
-
                        <img src="https://static.igem.org/mediawiki/2014/2/22/Logo2014v2.png" height="30" width="80">
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                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Protocol/Notebooks">Protocol/Notebooks</a></dd>
-
                    </a>
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                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Biobricks">Biobricks</a></dd>
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                     <a class="navbar-brand" href="https://2014.igem.org/Team:NJAU_China/">NJAU_China</a></div>
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                </dl>
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                <div class="collapse navbar-collapse">
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          </li>
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                     <ul class="nav navbar-nav">
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          <li id="prctice_li"><a href="#">Practice</a>
-
 
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                <dl id="prctice">
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                        <li class="active"><a href="https://2014.igem.org/Team:NJAU_China" class="unselect">Home</a></li>
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                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Exhibition and Workshops">Exhibitions and Workshops</a></dd>
-
                        <li class="dropdown"><a href="#" class="dropdown-toggle" data-toggle="dropdown">Project<span
+
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Questionnaire Survey">Questionnaire Survey</a></dd>
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                                class="caret"></span></a>
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                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Collaboration">Collaboration</a></dd>
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                            <ul class="dropdown-menu" role="menu">
+
                </dl>
-
                                <li><a href="https://2014.igem.org/Team:NJAU_China/Background">Background</a></li>
+
          </li>
-
                                <li><a href="https://2014.igem.org/Team:NJAU_China/System Design">System Design</a></li>
+
          <li><a href="https://2014.igem.org/Team:NJAU_China/Team">Team</a></li>
-
                                <li><a href="https://2014.igem.org/Team:NJAU_China/Safety">Safety</a></li>
+
          <li id="ackonwledgement_li"><a href="#">Acknowledgement</a>
-
                            </ul>
+
                <dl id="ackonwledgement">
-
                        </li>
+
                      <dd><a href="https://2014.igem.org/Team:NJAU_China/Inside Campus">Inside Campus&Arrtibutions</a></dd>
-
                        <li class="dropdown"><a href="#" class="dropdown-toggle" data-toggle="dropdown">Achievement<span
+
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Outside Campus">Outside Campus</a></dd>
-
                                class="caret"></span></a>
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                </dl>
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+
          </li>
-
                                <li><a href="https://2014.igem.org/Team:NJAU_China/Modeling">Modeling</a></li>
+
      </ul>
-
                                <li><a href="https://2014.igem.org/Team:NJAU_China/Biobricks">Biobricks</a></li>
+
-
                                <li><a href="https://2014.igem.org/Team:NJAU_China/Judging">Judging</a></li>
+
-
                            </ul>
+
-
                        </li>
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-
                        <li><a href="https://2014.igem.org/Team:NJAU_China/Human Practice" class="unselect">Human Practice</a></li>
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                        <li><a href="#" class="dropdown-toggle" data-toggle="dropdown">Wet Lab<span
+
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                                class="caret"></span></a>
+
-
                            <ul class="dropdown-menu" role="menu">
+
-
                                <li><a href="https://2014.igem.org/Team:NJAU_China/Biobricks">Biobricks</a></li>
+
-
                            </ul>
+
-
                        </li>
+
-
                        <li><a href="https://2014.igem.org/Team:NJAU_China/Team" class="unselect">Team</a></li>
+
-
                        <li><a href="https://2014.igem.org/Team:NJAU_China/Acknowledgement" class="unselect">Acknowledgement</a>
+
-
                        </li>
+
-
                    </ul>
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    <h2>Abstract</h2>
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    <p>As an common pollutant source of industry, low concentration of copper ions can do harm to many creatures. Whereas, <i>Escherichia coli</i> has high tolerance for copper through specific systems. There are <i>cop</i>A promoter,<i>mar</i>O（operator of mar locus） and <i>cue</i>O promoter existing in <i>E.Coli</i>, all of which are directly or indirectly regulated by copper ions. The most sensitive and effective one (<i>cop</i>A)was chosen to trigger the sequential reactions. Glutathione is an intracellular redox-active tripeptide thiol which can effectively detoxify the metal ions by chelation. It can be synthesized by <i>gsh</i>F, an coding sequence derived from <i>Streptococcus thermophilus</i>, which can unify both necessary reactions in one platform.In addition, we also utilize sgRNA-dCas9 system to enhance the efficiency of chelate cupric and cuprous by repressing the expression of <i>cop</i>A. With all these design, an automatically and efficiently copper-preventing guard have been created successfully. </p>
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             <div class="item active text-center"><img src="https://static.igem.org/mediawiki/2014/d/d3/Njau_slide1.jpg"
             <div class="item active text-center"><img src="https://static.igem.org/mediawiki/2014/d/d3/Njau_slide1.jpg"
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                                                       alt="First slide" class="text-center">
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             <div class="item text-center"><img src="https://static.igem.org/mediawiki/2014/3/38/Face.jpg"
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                        <h1>The First Teaching Building</h1>
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                        <p>Photoed by TianYC.</p>
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                        <p><a class="btn btn-lg btn-primary" style="color:#FFF" href="http://english.njau.edu.cn/"
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                              role="button">Learn more</a></p>
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                        <h1>Quadripod Ding</h1>
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                        <p>A landmark of Nanjing Agricultural University, which means NJAU was one branch of National
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                            Central University</p>
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                        <p><a class="btn btn-lg btn-primary" style="color:#FFF"
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                              href="http://english.njau.edu.cn/html/About/History/1.html" role="button">Learn more</a>
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                        <h1>Photo of team members</h1>
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                        <p>Exclude two people who are abroad</p>
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                        <p><a class="btn btn-lg btn-primary" style="color:#FFF" href="http://lfc.njau.edu.cn:8026/"
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    <!-- START THE FEATURETTES -->
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            <li id="one_1"><a href="https://2014.igem.org/Team:NJAU_China/Background" id="one"></a></li>
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                    class="text-muted">It'll blow your mind.</span></h2>
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            <p class="lead">Donec ullamcorper nulla non metus auctor fringilla. Vestibulum id ligula porta felis euismod
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                 commodo.</p>
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            <h2 class="featurette-heading">Oh yeah, it's that good. <span class="text-muted">See for yourself.</span>
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<div id="thanks">
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<img src="https://static.igem.org/mediawiki/2014/2/26/Njau_bg.png" alt="njau"><img src="https://static.igem.org/mediawiki/2014/a/ae/Shengke_bg.png" alt="shengke">
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                commodo.</p>
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            <h2 class="featurette-heading">And lastly, this one. <span class="text-muted">Checkmate.</span></h2>
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                commodo.</p>
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    <footer>
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        <p class="pull-right"><a href="#">Back to top</a></p>
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        <p>&copy;  NJAU@2014 iGEM. &middot;</p>
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Latest revision as of 17:03, 19 November 2014

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Abstract

As an common pollutant source of industry, low concentration of copper ions can do harm to many creatures. Whereas, Escherichia coli has high tolerance for copper through specific systems. There are copA promoter,marO（operator of mar locus） and cueO promoter existing in E.Coli, all of which are directly or indirectly regulated by copper ions. The most sensitive and effective one (copA)was chosen to trigger the sequential reactions. Glutathione is an intracellular redox-active tripeptide thiol which can effectively detoxify the metal ions by chelation. It can be synthesized by gshF, an coding sequence derived from Streptococcus thermophilus, which can unify both necessary reactions in one platform.In addition, we also utilize sgRNA-dCas9 system to enhance the efficiency of chelate cupric and cuprous by repressing the expression of copA. With all these design, an automatically and efficiently copper-preventing guard have been created successfully.

njaushengke

Retrieved from "http://2014.igem.org/Team:NJAU_China"