Team:OUC-China

From 2014.igem.org

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Line 1: Line 1:
{{CSS/Main}}
{{CSS/Main}}
<html>
<html>
-
    <link href="https://2014.igem.org/Team:OUC-China/css/bootstrap.css?action=raw&amp;ctype=text/css" rel="stylesheet" type="text/css" />
+
<link href="https://2014.igem.org/Team:OUC-China/css/bootstrap.css?action=raw&amp;ctype=text/css" rel="stylesheet" />
-
     <link href="https://2014.igem.org/Team:OUC-China/css/topsection.css?action=raw&ctype=text/css" rel="stylesheet" type="text/css" />
+
     <link href="https://2014.igem.org/Team:OUC-China/css/topsection.css?action=raw&ctype=text/css" rel="stylesheet" />
     <script src="https://2014.igem.org/Team:OUC-China/js/jquery.js?action=raw&ctype=text/javascript"></script>
     <script src="https://2014.igem.org/Team:OUC-China/js/jquery.js?action=raw&ctype=text/javascript"></script>
     <script src="https://2014.igem.org/Team:OUC-China/js/bootstrap.js?action=raw&ctype=text/javascript"></script>
     <script src="https://2014.igem.org/Team:OUC-China/js/bootstrap.js?action=raw&ctype=text/javascript"></script>
-
    <script src="https://2014.igem.org/Team:OUC-China/js/unslider.js?action=raw&ctype=text/javascript"></script>
+
<script type="text/javascript" src="https://2014.igem.org/Team:OUC-China/js/jquery.devrama.slider.js?action=raw&ctype=text/javascript"></script>
-
<style>
+
<script src="https://2014.igem.org/Team:OUC-China/js/unslider.js?action=raw&ctype=text/javascript"></script>
-
         #silder,#silder > ul, #silder > ul > li {
+
<style type="text/css">
 +
a:hover { text-decoration: none !important; }
 +
@charset "utf-8";
 +
/* CSS Document */
 +
body, div, p, ul, li, h1, h2, h3, h4, h5, h6 {
 +
margin: 0px;
 +
padding: 0px;
 +
font-family: "微软雅黑";
 +
}
 +
body{
 +
    min-width:1340px;
 +
}
 +
ul, ol {
 +
list-style: none;
 +
}
 +
img {
 +
display: block;
 +
position: absolute;
 +
}
 +
a {
 +
color: inherit;
 +
text-decoration: none;
 +
}
 +
@charset "utf-8";
 +
/* CSS Document */
 +
@keyframes bubleM {
 +
0% {
 +
margin-top:0px;
 +
margin-left:0px;
 +
}
 +
25% {
 +
margin-top:3px;
 +
margin-left:3px;
 +
}
 +
50% {
 +
margin-top:6px;
 +
margin-left:0px;
 +
}
 +
75% {
 +
margin-top:3px;
 +
margin-left:-3px;
 +
}
 +
100% {
 +
margin-top:0px;
 +
margin-left:0px;
 +
}
 +
}
 +
@-webkit-keyframes bubleM {
 +
0% {
 +
margin-top:0px;
 +
margin-left:0px;
 +
}
 +
25% {
 +
margin-top:3px;
 +
margin-left:3px;
 +
}
 +
50% {
 +
margin-top:6px;
 +
margin-left:0px;
 +
}
 +
75% {
 +
margin-top:3px;
 +
margin-left:-3px;
 +
}
 +
100% {
 +
margin-top:0px;
 +
margin-left:0px;
 +
}
 +
}
 +
.header {
 +
width: 1200px;
 +
height: 50px;
 +
margin: 0 auto;
 +
position: relative;
 +
}
 +
.header .igemLogo {
 +
transition: 0.5s;
 +
-moz-transition: 0.5s;
 +
-o-transition: 0.5s;
 +
-webkit-transition: 0.5s;
 +
}
 +
.header .igemLogo:hover {
 +
transform: rotate(360deg);
 +
-webkit-transform: rotate(360deg);
 +
-moz-transform: rotate(360deg);
 +
-o-transform: rotate(360deg);
 +
-ms-transform: rotate(360deg);
 +
}
 +
 
 +
.header .nav{
 +
top: 10px;
 +
left: 450px;
 +
}
 +
.header .Logo {
 +
width: 70px;
 +
height: 70px;
 +
background: url(https://static.igem.org/mediawiki/2014/f/fa/OUC-China_14Logo.png);
 +
position: absolute;
 +
right: 0;
 +
bottom: -10px;
 +
}
 +
.header .Logo ul {
 +
position: absolute;
 +
width: 250px;
 +
height: 150px;
 +
display: none;
 +
right: 0px;
 +
top: 70px;
 +
}
 +
.header .Logo li {
 +
height: 50px;
 +
width: 215px;
 +
line-height: 50px;
 +
font-size: 14px;
 +
background: #fff;
 +
margin: 10px 0 20px 0;
 +
position: relative;
 +
border-bottom-left-radius: 6px;
 +
border-top-left-radius: 6px;
 +
}
 +
.header .Logo li a {
 +
margin-left: 5px;
 +
}
 +
.header .Logo li img {
 +
position: absolute;
 +
right: -35px;
 +
top: -10px;
 +
}
 +
.neck {
 +
height: 512px;
 +
width: 100%;
 +
background: rgb(76,145,190);
 +
}
 +
.main {
 +
}
 +
.main .part1 {
 +
height: 480px;
 +
width: 100%;
 +
background: rgb(210,233,253);
 +
position: relative;
 +
}
 +
.main .part1 .waveUp {
 +
width: 100%;
 +
height: 130px;
 +
background: url(https://static.igem.org/mediawiki/2014/f/f0/WaveUp.png
 +
);
 +
position: absolute;
 +
bottom: 0;
 +
z-index: 0
 +
}
 +
.main .part1 .waveDown {
 +
width: 100%;
 +
height: 130px;
 +
background: url(https://static.igem.org/mediawiki/2014/6/63/WaveDown.png);
 +
position: absolute;
 +
bottom: 0;
 +
z-index: 3;
 +
}
 +
.main .part1 img {
 +
bottom: 60px;
 +
transition: 0.3s;
 +
-moz-transition: 0.3s;
 +
-o-transition: 0.3s;
 +
-webkit-transition: 0.3s;
 +
}
 +
.main .part1 img:hover {
 +
bottom: 80px;
 +
}
 +
.main .part1 .objLeft {
 +
left: 200px;
 +
}
 +
.main .part1 .objRight {
 +
right: 200px;
 +
}
 +
.main .part2 {
 +
width: 100%;
 +
height: 905px;
 +
background: rgb(96,159,210);
 +
position: relative;
 +
}
 +
.main .part2 ul li {
 +
position: absolute;
 +
display:none;
 +
}
 +
.main .part2 ul li p{
 +
display: block;
 +
position: absolute;
 +
width: 184px;
 +
color: rgb(49,129,194);
 +
text-align: center;
 +
font-size:24px;
 +
}
 +
.main .part2 .buble {
 +
animation-name: bubleM;
 +
animation-duration:1s;
 +
animation-timing-function: linear;
 +
animation-iteration-count: 10;
 +
animation-direction: alternate;
 +
animation-fill-mode: both;
 +
animation-play-state: running;
 +
-webkit-animation-name: bubleM;
 +
-webkit-animation-duration: 0.3s;
 +
-webkit-animation-timing-function: linear;
 +
-webkit-animation-iteration-count: 7;
 +
-webkit-animation-direction: alternate;
 +
-webkit-animation-fill-mode: both;
 +
-webkit-animation-play-state: running;
 +
}
 +
.delay1 {
 +
-moz-animation-delay: 0s;
 +
}
 +
.delay2 {
 +
-moz-animation-delay: 0.4s;
 +
}
 +
.delay3 {
 +
-moz-animation-delay: 0.8s;
 +
}
 +
.delay4 {
 +
-moz-animation-delay: 1.2s;
 +
}
 +
.delay5 {
 +
-moz-animation-delay: 1.6s;
 +
}
 +
.delay6 {
 +
-moz-animation-delay: 2s;
 +
}
 +
.main .part2 .lFish {
 +
left: 45px;
 +
bottom: -95px;
 +
}
 +
.main .part2 .rFish {
 +
right: 45px;
 +
bottom: -100px;
 +
}
 +
.footer {
 +
width: 100%;
 +
height: 220px;
 +
background: rgb(45,111,165);
 +
}
 +
@charset "utf-8";
 +
/* CSS Document */
 +
.nav {
 +
position: absolute;
 +
}
 +
.nav li {
 +
padding: 0 8px;
 +
float: left;
 +
position: relative;
 +
min-width: 80px;
 +
height: 40px;
 +
color: rgb(60,60,60);
 +
font-size: 24px;
 +
line-height: 40px;
 +
text-align: center;
 +
transition: 0.3s;
 +
-moz-transition: 0.3s;
 +
-o-transition: 0.3s;
 +
-webkit-transition: 0.3s;
 +
}
 +
.nav li:hover {
 +
background: rgb(146,205,253);
 +
color: #fff;
 +
}
 +
.nav .pMenu ul {
 +
display: none;
 +
position: absolute;
 +
top: 100%;
 +
left: 0px;
 +
overflow: hidden;
 +
border-bottom-left-radius: 6px;
 +
border-bottom-right-radius: 6px;
 +
}
 +
.nav .pMenu ul li {
 +
min-width: 220px;
 +
background: rgb(146,205,253);
 +
color: #fff;
 +
transition: 0.4s;
 +
-moz-transition: 0.4s;
 +
-o-transition: 0.4s;
 +
-webkit-transition: 0.4s;
 +
}
 +
.nav .pMenu ul li:hover {
 +
background: rgb(98,175,243);
 +
}
 +
.neck{ background-image:url(https://static.igem.org/mediawiki/2014/2/20/OUC-China_Index_Slide1.png)}
 +
.example-animation {
 +
color: #FFF;
 +
font-size: 60px;
 +
}
 +
header {
 +
            position: absolute;
 +
            width: 1020px;
 +
            margin: 0 auto;
 +
            z-index: 999;
 +
        }
 +
        header > ul {
 +
            list-style: none;
 +
            display: inline-block;
 +
            margin-left: 10px;
 +
            margin-right: 10px;float:left;
 +
            -webkit-transition: all ease 0.5s;
 +
        }
 +
        header > ul:hover {
 +
            margin-top: 0 !important;
 +
        }
 +
        header > ul > li {
 +
            width: 150px;
 +
            min-height: 50px;
 +
            padding: 10px;
 +
            background-color: #ffffff;
 +
        }
 +
        header > ul > li > a {
 +
            display: block;
 +
            font-size: 16px;
 +
            text-align: center;
 +
            cursor: pointer;
 +
            color: #1d89f7;
 +
        }
 +
        header > ul > li > a:hover {
 +
            color: #000000;
 +
            text-decoration: none;
 +
        }
 +
        header > ul > li:last-child {
 +
            height: 70px;
 +
            width: 70px;
 +
            border-radius: 50%;
 +
            margin-left: 40px;
 +
            margin-top: -10px;
 +
        }
 +
        header > ul > li:last-child > div {
 +
            width: 50px;
 +
            height: 50px;
 +
            border: 5px solid #1d89f7;
 +
            border-radius: 50%;
 +
        }
 +
        header > ul > li:last-child > div > div {
 +
            width: 0;
 +
            height: 0;
 +
            margin-left: 5px;
 +
            margin-top: 5px;
 +
            border-radius: 50%;
 +
            border: 15px solid #1d89f7;
 +
        }
 +
        .logo {
 +
            position: absolute;
 +
            top: 10px;
 +
            z-index: 10;
 +
        }
 +
#slider {
 +
            background-image: url(https://static.igem.org/mediawiki/2014/2/20/OUC-China_Index_Slide1.png);
 +
            background-repeat: no-repeat;
 +
            background-size: cover;
 +
        }
 +
         #slider,#slider > ul, #slider > ul > li {
             width:100%;
             width:100%;
-
             height:362px;
+
             height:615px;
         }
         }
-
         #silder > ul {
+
         #slider > ul {
             margin: 0;
             margin: 0;
             padding: 0;
             padding: 0;
         }
         }
-
         #silder > ul > li {
+
         #slider > ul > li {
             list-style: none;
             list-style: none;
         }
         }
-
             #silder > .dots {
+
             #slider > .dots {
                 position: relative;
                 position: relative;
                 left: 0;
                 left: 0;
Line 26: Line 379:
                 margin: 0 !important;
                 margin: 0 !important;
             }
             }
-
                 #silder > .dots > li {
+
                 #slider > .dots > li {
                     display: inline-block;
                     display: inline-block;
                     width: 10px;
                     width: 10px;
Line 40: Line 393:
                     transition: background .5s, opacity .5s;
                     transition: background .5s, opacity .5s;
                 }
                 }
-
                 #silder > .dots > li.active {
+
                 #slider > .dots > li.active {
                     background: #5b97fb;
                     background: #5b97fb;
                     opacity: 1;
                     opacity: 1;
                 }
                 }
-
         .team {
+
         @-webkit-keyframes CircleLogo {
-
             height: 481px;
+
             0% {
-
             background-color: #d2e9fd;
+
                transform: rotate(0deg);
 +
             }
 +
            50% {
 +
                transform: rotate(180deg);
 +
            }
 +
            100% {
 +
                transform: rotate(360deg);
 +
            }
         }
         }
-
         .team > div:first-child {
+
         @-moz-keyframes CircleLogo {
-
             width: 1024px;
+
             0% {
-
             margin: 0 auto;
+
                transform: rotate(0deg);
 +
             }
 +
            50% {
 +
                transform: rotate(180deg);
 +
            }
 +
            100% {
 +
                transform: rotate(360deg);
 +
            }
         }
         }
-
         .team .nami {
+
         @-ms-keyframes CircleLogo {
-
             position: relative;
+
             0% {
-
             height: 130px;
+
                transform: rotate(0deg);
-
             background-repeat: repeat-x;
+
             }
 +
            50% {
 +
                transform: rotate(180deg);
 +
             }
 +
            100% {
 +
                transform: rotate(360deg);
 +
            }
         }
         }
-
         .team .plas {
+
         @keyframes CircleLogo {
-
             margin-top: 240px;
+
             0% {
-
             position: relative;
+
                transform: rotate(0deg);
-
             z-index: 5;
+
             }
-
            -webkit-transition: all ease 0.4s;
+
             50% {
-
             -moz-transition: all ease 0.4s;
+
                transform: rotate(180deg);
-
             -ms-transition: all ease 0.4s;
+
             }
-
            -o-transition: all ease 0.4s;
+
             100% {
-
             transition: all ease 0.4s;
+
                transform: rotate(360deg);
-
        }
+
             }
-
        .team .plas:hover {
+
-
            margin-top:230px;
+
         }
         }
-
        .part {
+
</style>
-
            height: 850px;
+
<script>
-
            background-color: #609fd2;
+
-
            margin-top: -1px;
+
-
        }
+
-
        .part > ul {
+
-
          width: 1024px;
+
-
          list-style: none;
+
-
          margin: 0  auto;
+
-
        }
+
-
        .part > ul > li {
+
-
            width: 440px;
+
-
            height: 235px;
+
-
            float:left;
+
-
            margin: 20px;
+
-
            background-color: #ffffff;
+
-
        }
+
-
        .part > ul > li > p:first-child {
+
-
            display: block;
+
-
            width: 100%;
+
-
            padding: 5px;
+
-
            height: 50px;
+
-
        }
+
-
        .part > ul > li > p:first-child > img {
+
-
            display: inline-block;
+
-
            width: 40px;
+
-
            height: 40px;
+
-
            margin-top: -20px;
+
-
        }
+
-
        .part > ul > li > p:first-child > span {
+
-
            font-size: 30px;
+
-
            padding-top: 10px;
+
-
            padding-left: 10px;
+
-
            color: #222222;
+
-
            display: inline-block;
+
-
        }
+
-
        footer {
+
-
            width: 100%;
+
-
            height: 100px;
+
-
            background-color: #2d6fa5;
+
-
        }
+
-
        footer p {
+
-
            color: #ffffff;
+
-
            font-size: 14px;text-align: right;
+
-
            padding-right: 20px;
+
-
        }
+
-
        footer p a {
+
-
            color: #ffffff !important;
+
-
        }
+
-
        footer p a:hover {
+
-
            color: #c7f1ff !important;
+
-
        }
+
-
        .anchor {
+
-
            position: fixed;
+
-
            right: 10px;
+
-
            bottom: 10px;
+
-
            cursor: pointer;
+
-
            width: 60px;
+
-
            height: 60px;
+
-
            z-index: 20;
+
-
            -webkit-transition: all ease 0.4s;
+
-
            -moz-transition: all ease 0.4s;
+
-
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             <img class="plas" src="https://static.igem.org/mediawiki/2014/4/41/OUC-China_Index_Plasmid1.png" style="float:left" />
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            <li><a href="https://2014.igem.org/Team:OUC-China/Project">Overview</a></li>
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            <li><a href="https://2014.igem.org/Team:OUC-China/Judging">Biobrick</a></li>
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            <li><a href="https://2014.igem.org/Team:OUC-China/Judging_Achievment">Achievement</a></li>
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            <li><a href="https://2014.igem.org/Team:OUC-China/Notebook">Lab Note</a></li>
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            <li><a href="https://2014.igem.org/Team:OUC-China/Notebook_Modeling_Note">Modeling Note</a></li>
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            <li><a href="https://2014.igem.org/Team:OUC-China/Notebook_Protocols">Protocols</a></li>
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                    <img src="https://static.igem.org/mediawiki/2014/9/9c/OUC-China_Index_Part1.png" />
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                    <span>The double plasmid system</span>
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                </p>
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             <li>
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<div style="width:5px;height:100%;position:relative;background-color:#ffffff;margin-left:49%;transform: rotate(10deg);-webkit-transform: rotate(10deg);-moz-transform: rotate(10deg);-ms-transform: rotate(10deg);margin-top:-600px;"></div>
-
                    <span>Transfection</span>
+
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                </p>
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             </li>
             <li>
             <li>
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                    <img src="https://static.igem.org/mediawiki/2014/9/98/OUC-China_Index_Part3.png" />
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                    <span>Modeling</span>
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                    <img src="https://static.igem.org/mediawiki/2014/4/44/OUC-China_Index_Part4.png" />
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                    <span>Judging</span>
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                    <span>Safety</span>
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                    <span>Policy&practise</span>
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<div class = "main">
 +
  <div class = "part1">
 +
<h2 class="text-primary" style="width: 960px;margin:0 auto;padding-top:10px;">Introduction</h2>
 +
<p style="width: 960px;margin:0 auto;font-size: 19px;padding-top:12px;">Current researches usually transfer exogenous DNA into prokaryotic cells and eukaryotic cells, while OUC-China iGEM team has designed an innovative method to carry exogenous DNA from prokaryote to eukaryota more efficiently. Eventually we have applied the method to studies of the model organism, zebrafish. <br />
 +
We have constructed the fusion protein that can bind and protect plasmids, with the ability of helping plasmids transfect into eukaryotic cells in high efficiency.</p>
 +
<a href="https://2014.igem.org/Team:OUC-China/Project" class="btn btn-primary btn-lg" style="display:block;margin:40px auto;width:200px;position:relative;color: #ffffff;">Learn More</a>
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     <img class = "objLeft" src="https://static.igem.org/mediawiki/2014/4/41/OUC-China_Index_Plasmid1.png"/> <img class = "objRight" src="https://static.igem.org/mediawiki/2014/e/e6/OUC-China_Index_Plasmid2.png"/> </div>
 +
  <div class = "part2"> <img class = "lFish" src="https://static.igem.org/mediawiki/2014/a/a0/OUC-China_FishLeft.png"/> <img class = "rFish" src="https://static.igem.org/mediawiki/2014/d/de/OUC-China_FishRight.png"/>
 +
    <ul style="width:1350px; margin:auto; position:relative;">
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      <li style = "top:85px;left:145px;-webkit-animation-duration: 0.3s;"> <img src="https://static.igem.org/mediawiki/2014/4/45/OUC-China_1.png"/> <img id = "lGear" src="https://static.igem.org/mediawiki/2014/d/d0/LGear.png" style="top:59px;left:131px;display:none;"/> <img src="https://static.igem.org/mediawiki/2014/f/f5/OUC-China_BGear.png" style="top:75px;left:25px;"/>
 +
        <p style="top: 200px; left: 70px;"><a href="https://2014.igem.org/Team:OUC-China/Project_Design#Double_Plamisd">The Double Plasmid System</a></p>
 +
      </li>
 +
      <li style = "top:30px;left:520px;-webkit-animation-duration: 0.33s;"><img src="https://static.igem.org/mediawiki/2014/6/61/OUC-China2.png"/> <img src="https://static.igem.org/mediawiki/2014/9/91/OUC-China_Circle.png" style="top: 69px; left: 38px;"> <img id = "lGear2" src="https://static.igem.org/mediawiki/2014/d/d0/LGear.png" style="top: 34px; left: 179px;">
 +
        <p style="top: 275px; left: 86px;"><a href="https://2014.igem.org/Team:OUC-China/Project_Design#Transfection">Transfection</a></p>
 +
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  <p>contact us: <a href="mailto:oucigem@163.com">oucigem@163.com</a></p>
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Latest revision as of 03:59, 18 October 2014

Introduction

Current researches usually transfer exogenous DNA into prokaryotic cells and eukaryotic cells, while OUC-China iGEM team has designed an innovative method to carry exogenous DNA from prokaryote to eukaryota more efficiently. Eventually we have applied the method to studies of the model organism, zebrafish.
We have constructed the fusion protein that can bind and protect plasmids, with the ability of helping plasmids transfect into eukaryotic cells in high efficiency.

Learn More

contact us: oucigem@163.com

Retrieved from "http://2014.igem.org/Team:OUC-China"