Team:Tuebingen/Notebook/Protocols/TBE buffer

From 2014.igem.org

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<h1>Protocols</h1>
<h3>TBE buffer</h3>
<h3>TBE buffer</h3>
<h4>Reagents</h4>
<h4>Reagents</h4>
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     <td style="text-align: center">0.85 M</td>
     <td style="text-align: center">0.85 M</td>
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     <td>B(OH)3</td>
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     <td>B(OH)<sub>3</sub></td>
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<p>&nbsp;</p>
<h4>Procedure</h4>
<h4>Procedure</h4>
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<p>Add the reagents to 1000 ml H20. Adjust to pH 8.3 with B(OH)3.</p>
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<p>Add the reagents to 1000 ml H<sub>2</sub>0. Adjust to pH 8.3 with B(OH)<sub>3</sub>.</p>

Latest revision as of 15:28, 17 October 2014


Protocols

TBE buffer

Reagents

1 M Tris
0.85 M B(OH)3
1 M EDTA 0.5 M pH 8.0

 

Procedure

Add the reagents to 1000 ml H20. Adjust to pH 8.3 with B(OH)3.