Team:Tuebingen/Notebook/Protocols/miniprep

From 2014.igem.org

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<h1>Protocols</h1>
<h3>Preparation of plasmids on a small scale</h3>
<h3>Preparation of plasmids on a small scale</h3>
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<h4>Procedure</h4>
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<ol>
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  <li> Pick a single colony from a plate, give it in a a 15 ml Falcon-tube with 10 ml LB-medium and incubate at 37 °C and overnight.</li>
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  <li> Pelletize 3 ml of the overnight-culture and discard the supernatant.</li>
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  <li> Continue according to the Quiagen QIAprep ® Spin Miniprep Kit Manual or the Biozyme EasyPrep ® Pro Plasmid Miniprep Kit Manual.</li>
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  <li> Store at – 20 °C.</li>
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</ol>
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<p>&nbsp;</p>
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<h4>Kits</h4>
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<ul>
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  <li><a href="http://www.qiagen.com/resources/resourcedetail?id=331740ca-077f-4ddd-9e5a-2083f98eebd5&lang=en">Quiagen QIAprep ® Spin Miniprep Kit</a></li>
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  <li><a href="http://www.biozym.com/desktopmodules/webshop/downloads/390000_EasyPrepProManual0608.pdf">Biozyme EasyPrep ® Pro Plasmid Miniprep Kit</a></li>
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</ul>
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</div>
</div>

Latest revision as of 15:26, 17 October 2014


Protocols

Preparation of plasmids on a small scale

Procedure

  1. Pick a single colony from a plate, give it in a a 15 ml Falcon-tube with 10 ml LB-medium and incubate at 37 °C and overnight.
  2. Pelletize 3 ml of the overnight-culture and discard the supernatant.
  3. Continue according to the Quiagen QIAprep ® Spin Miniprep Kit Manual or the Biozyme EasyPrep ® Pro Plasmid Miniprep Kit Manual.
  4. Store at – 20 °C.

 

Kits

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