Team:Tuebingen/Notebook/Protocols/LB agar

From 2014.igem.org

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<div id="TueContent">
<div id="TueContent">
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<h1>Protocols</h1>
<h3>LB agar</h3>
<h3>LB agar</h3>
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<h4>Reagents</h4>
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<table border="0">
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  <colgroup>
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    <col width="100">
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    <col width="300">
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  </colgroup>
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  <tr>
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    <td style="text-align: center">10 g</td>
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    <td>Bacto-tryptone</td>
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  </tr>
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  <tr>
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    <td style="text-align: center">5 g</td>
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    <td>yeast extract</td>
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  </tr>
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  <tr>
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    <td style="text-align: center">10 g</td>
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    <td>NaCl</td>
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  </tr>
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</table>
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<p>&nbsp;</p>
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<h4>Procedure</h4>
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<ol>
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  <li> Add the reagents to 800 ml H<sub>2</sub>O</li>
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  <li> Adjust pH to 7.5 with NaOH</li>
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  <li> Add 15g agar</li>
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  <li> Melt agar into solution in the microwave</li>
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  <li> Adjust volume to 1 L with dH<sub>2</sub>O</li>
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  <li> Sterilize by autoclaving</li>
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</ol>
</div>
</div>

Latest revision as of 19:40, 17 October 2014


Protocols

LB agar

Reagents

10 g Bacto-tryptone
5 g yeast extract
10 g NaCl

 

Procedure

  1. Add the reagents to 800 ml H2O
  2. Adjust pH to 7.5 with NaOH
  3. Add 15g agar
  4. Melt agar into solution in the microwave
  5. Adjust volume to 1 L with dH2O
  6. Sterilize by autoclaving

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