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Team:Tuebingen/Notebook/Protocols/gelelectrophoresis
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Fill the wells with desired amount of DNA mixed with the 6x loading dye (to end up with a 1x | Fill the wells with desired amount of DNA mixed with the 6x loading dye (to end up with a 1x | ||
loading dye).</p> | loading dye).</p> | ||
| - | <p>Start the gel electrophoresis by applying 100-120 V and stop | + | <p>Start the gel electrophoresis by applying 100-120 V and stop when marker reaches the bottom of |
the gel.</p> | the gel.</p> | ||
Latest revision as of 19:38, 17 October 2014
Protocols
Agarose gel electrophoresis
Reagents
| 1.0 g | Agarose |
| 100 mL | 1x TAE buffer |
| 4 µL | Midori Green solution |
| x µL | 6x Loading dye |
| 5 µL | DNA-Ladder Mix |
| 5-15 µL | DNA sample |
Procedure
Add agarose to TAE buffer and heat in microwave until the agarose is completely dissolved. Cool down the solution until it is about 40 °C and pour it in the gel electrophoresis apparatus. Add 4 μl Midori Green to the poured gel, mix the suspension and add the comb. Place the solid gel in the gel electrophoresis apparatus (with the wells near the cathode) and fill with 1x TAE buffer. Fill the wells with desired amount of DNA mixed with the 6x loading dye (to end up with a 1x loading dye).
Start the gel electrophoresis by applying 100-120 V and stop when marker reaches the bottom of the gel.
