Team:UMaryland/project/futureapplications
From 2014.igem.org
(Difference between revisions)
(Created page with "{{UMaryland/CSS}} <html xml:lang="en-gb" lang="en-gb" dir="ltr" class="bootstrap2 itemid-254 com_content view-article"> <head> <base href="https://2014.igem.org/Team:UMaryland/...") |
|||
(7 intermediate revisions not shown) | |||
Line 8: | Line 8: | ||
<meta name="viewport" content="width=device-width, initial-scale=1.0, maximum-scale=1.0, user-scalable=no" /> | <meta name="viewport" content="width=device-width, initial-scale=1.0, maximum-scale=1.0, user-scalable=no" /> | ||
<title>future applications</title> | <title>future applications</title> | ||
- | + | <link href="http://gdriv.es/igemhost/iGemWiki/templates/baseline/favicon.ico" rel="shortcut icon" type="image/vnd.microsoft.icon" /> | |
<link rel="stylesheet" href="http://gdriv.es/igemhost/iGemWiki/plugins/system/yjsg/assets/css/font-awesome.min.css" type="text/css" /> | <link rel="stylesheet" href="http://gdriv.es/igemhost/iGemWiki/plugins/system/yjsg/assets/css/font-awesome.min.css" type="text/css" /> | ||
<link rel="stylesheet" href="http://gdriv.es/igemhost/iGemWiki/templates/baseline/css_compiled/bootstrap-style1.css" type="text/css" /> | <link rel="stylesheet" href="http://gdriv.es/igemhost/iGemWiki/templates/baseline/css_compiled/bootstrap-style1.css" type="text/css" /> | ||
Line 63: | Line 63: | ||
<div id="header"> | <div id="header"> | ||
<div id="logo"> | <div id="logo"> | ||
- | <a href=" | + | <a href="https://2014.igem.org/Main_Page"></a> |
</div> | </div> | ||
<!-- end logo --> | <!-- end logo --> | ||
Line 71: | Line 71: | ||
<div class="top_menu"> | <div class="top_menu"> | ||
<div id="horiznav" class="horiznav"> <ul class="megalegacy menunav"> | <div id="horiznav" class="horiznav"> <ul class="megalegacy menunav"> | ||
- | <li class="item101 level0 first"><span class="mymarg"><a class="yjanchor first" href="/ | + | <li class="item101 level0 first"><span class="mymarg"><a class="yjanchor first" href="https://2014.igem.org/Team:UMaryland"><span class="yjm_has_none"><span class="yjm_title">Homepage</span></span></a></span></li><li class="item104 level0"><span class="mymarg"><a class="yjanchor " href="https://2014.igem.org/Team:UMaryland/photos"><span class="yjm_has_none"><span class="yjm_title">Photos</span></span></a></span></li><li class="haschild item105 level0"><span class="child"><a class="yjanchor " href="https://2014.igem.org/Team:UMaryland/meetourteam"><span class="yjm_has_none"><span class="yjm_title">Team</span></span></a></span><ul class="subul_main level1"><li class="bl"></li><li class="tl"></li><li class="tr"></li><li class="item293 level1 first"><span class="mymarg"><a class="yjanchor first" href="https://2014.igem.org/Team:UMaryland/meetourteam"><span class="yjm_has_none"><span class="yjm_title">Meet Our Team Members</span></span></a></span></li><li class="item116 level1"><span class="mymarg"><a class="yjanchor " href="https://igem.org/Team.cgi?id=1489"><span class="yjm_has_none"><span class="yjm_title">iGEM Page</span></span></a></span></li><li class="item117 level1"><span class="mymarg"><a class="yjanchor " href="https://2014.igem.org/Team:UMaryland/team/attributions"><span class="yjm_has_none"><span class="yjm_title">Attributions</span></span></a></span></li><li class="item300 level1 lilast"><span class="mymarg"><a class="yjanchor last" href="https://2014.igem.org/Team:UMaryland/team/sponsors"><span class="yjm_has_none"><span class="yjm_title">Sponsors</span></span></a></span></li><li class="right"></li><li class="br"></li></ul></li><li class=" active haschild item112 level0"><span class="child"><a class="yjanchor activepath yjscroll" href="https://2014.igem.org/Team:UMaryland/project/overview"><span class="yjm_has_none"><span class="yjm_title">Project</span></span></a></span><ul class="subul_main level1"><li class="bl"></li><li class="tl"></li><li class="tr"></li><li class="item138 level1 first"><span class="mymarg"><a class="yjanchor first" href="https://2014.igem.org/Team:UMaryland/project/overview"><span class="yjm_has_none"><span class="yjm_title">Overview</span></span></a></span></li><li class="item145 level1"><span class="mymarg"><a class="yjanchor " href="https://2014.igem.org/Team:UMaryland/project/background"><span class="yjm_has_none"><span class="yjm_title">Background</span></span></a></span></li><li class="item144 level1"><span class="mymarg"><a class="yjanchor " href="https://2014.igem.org/Team:UMaryland/project/biobricks"><span class="yjm_has_none"><span class="yjm_title">parts (BioBricks)</span></span></a></span></li><li class="item102 level1"><a class="yjanchor " href="https://2014.igem.org/Team:UMaryland/project/results" target="_blank" ><span class="yjm_has_none"><span class="yjm_title">Results</span></span></a></li><li id="current" class=" active item254 level1"><span class="mymarg"><a class="yjanchor activepath " href="https://2014.igem.org/Team:UMaryland/project/futureapplications"><span class="yjm_has_none"><span class="yjm_title">future applications</span></span></a></span></li><li class="item301 level1 lilast"><span class="mymarg"><a class="yjanchor last" href="https://igem.org/Safety/Safety_Form?team_id=1489"><span class="yjm_has_none"><span class="yjm_title">Safety</span></span></a></span></li><li class="right"></li><li class="br"></li></ul></li><li class="haschild item234 level0"><span class="child"><a class="yjanchor " href="https://2014.igem.org/Team:UMaryland/notebook"><span class="yjm_has_none"><span class="yjm_title">Notebook</span></span></a></span><ul class="subul_main level1"><li class="bl"></li><li class="tl"></li><li class="tr"></li><li class="item303 level1 first lilast"><span class="mymarg"><a class="yjanchor firstlast" href="https://2014.igem.org/Team:UMaryland/notebook"><span class="yjm_has_none"><span class="yjm_title">Wetlab Notebook</span></span></a></span></li><li class="right"></li><li class="br"></li></ul></li><li class="haschild item123 level0"><span class="child"><a class="yjanchor " href="https://2014.igem.org/Team:UMaryland/humanprac/overview"><span class="yjm_has_none"><span class="yjm_title">Human Practices</span></span></a></span><ul class="subul_main level1"><li class="bl"></li><li class="tl"></li><li class="tr"></li><li class="item106 level1 first"><span class="mymarg"><a class="yjanchor first" href="https://2014.igem.org/Team:UMaryland/humanprac/overview"><span class="yjm_has_none"><span class="yjm_title">Overview | Mission Statement</span></span></a></span></li><li class="haschild item129 level1"><span class="child subparent"><a class="yjanchor grouptitle" href="https://2014.igem.org/Team:UMaryland/humanprac/publicschool"><span class="yjm_has_none"><span class="yjm_title">Public School Outreach</span></span></a></span><ul class="subul_main level2"><li class="bl"></li><li class="tl"></li><li class="tr"></li><li class="item134 level2 first lilast"><span class="mymarg"><a class="yjanchor firstlast" href="https://2014.igem.org/Team:UMaryland/humanprac/publicschool"><span class="yjm_has_none"><span class="yjm_title">HighSchool</span></span></a></span></li><li class="right"></li><li class="br"></li></ul></li><li class="item131 level1"><span class="mymarg"><a class="yjanchor " href="https://2014.igem.org/Team:UMaryland/humanprac/community"><span class="yjm_has_none"><span class="yjm_title">Community Interaction</span></span></a></span></li><li class="item132 level1 lilast"><span class="mymarg"><a class="yjanchor last" href="https://2014.igem.org/Team:UMaryland/humanprac/publicawareness"><span class="yjm_has_none"><span class="yjm_title">Public Awareness</span></span></a></span></li><li class="right"></li><li class="br"></li></ul></li></ul></div> |
</div> | </div> | ||
</div> | </div> | ||
Line 101: | Line 101: | ||
</a> | </a> | ||
</h1> | </h1> | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
Line 142: | Line 107: | ||
<p>We believe that the initial stages of the Biosensor have been complete and will lead to many new and exciting developments in the future. The general idea is that once the surface protein has been expressed, it will be able to interact with Dermo and produce a signal.</p> | <p>We believe that the initial stages of the Biosensor have been complete and will lead to many new and exciting developments in the future. The general idea is that once the surface protein has been expressed, it will be able to interact with Dermo and produce a signal.</p> | ||
<p>As for this year we our efforts working with a Bovine-Galectin have come the furthest and are ready to move into these next steps. Additionally in future years we hope to incorporate CvGal1 into the constructs, an effort we were not able to accomplish due to lack of time and troubleshooting. </p> | <p>As for this year we our efforts working with a Bovine-Galectin have come the furthest and are ready to move into these next steps. Additionally in future years we hope to incorporate CvGal1 into the constructs, an effort we were not able to accomplish due to lack of time and troubleshooting. </p> | ||
- | <p>Signaling Approaches:</p> | + | <p style="text-align: center;"><span style="text-decoration: underline;"><strong>Signaling Approaches:</strong></span></p> |
+ | <p> </p> | ||
<p>1). Genetic engineering approach -Downstream Signaling Cascade</p> | <p>1). Genetic engineering approach -Downstream Signaling Cascade</p> | ||
+ | <p><img src="https://static.igem.org/mediawiki/parts/9/99/UMSIgnal_Cascade.jpg" border="0" width="671" height="503" style="line-height: 12.0000009536743px; display: block; margin-left: auto; margin-right: auto; cursor: se-resize !important;" /></p> | ||
<p>One of the strategies we hope to pursue is developing or incorporating a downstream signaling cascade into the cell strain. In this effort, once the E. coli came in contact with the Dermo it would then set off a transcription factor, ultimately producing a signal. Multiple approaches have been researched, including a Cpx membrane stress cascade, a split GFP cascade, and others.</p> | <p>One of the strategies we hope to pursue is developing or incorporating a downstream signaling cascade into the cell strain. In this effort, once the E. coli came in contact with the Dermo it would then set off a transcription factor, ultimately producing a signal. Multiple approaches have been researched, including a Cpx membrane stress cascade, a split GFP cascade, and others.</p> | ||
- | <p>The Cpx two component system reacts naturally to membrane stress. When the much larger Dermo is bound to the surface receptor expressed on E. coli, the bacterial cell membrane would become deformed. In turn, this would transmit a signal to the nucleus via the | + | <p>The Cpx two component system reacts naturally to membrane stress. When the much larger Dermo is bound to the surface receptor expressed on E. coli, the bacterial cell membrane would become deformed. In turn, this would transmit a signal to the nucleus via the NlpE system that could be coupled to GFP production. This assay would require florescence microscopy to detect Dermo in solution.</p> |
<p>Another option is for a split GFP construct. The idea is two different constructs containing the ligand are present in the cell, each with a different fraction of the GFP gene. When Dermo comes in close contact with the cell, multiple ligands on the cell would bind to the receptors on Dermo, causing the membrane to pinch or come together. This would trigger a spontaneous chance that two complementary pieces of GFP in close contact would bind, producing a fluorescent signal. This would also need to be observed using microscopy.</p> | <p>Another option is for a split GFP construct. The idea is two different constructs containing the ligand are present in the cell, each with a different fraction of the GFP gene. When Dermo comes in close contact with the cell, multiple ligands on the cell would bind to the receptors on Dermo, causing the membrane to pinch or come together. This would trigger a spontaneous chance that two complementary pieces of GFP in close contact would bind, producing a fluorescent signal. This would also need to be observed using microscopy.</p> | ||
<p>One of the ultimate goals from using the downstream signaling approach is to develop a “Kamikaze Bacteria”. By this we hope that the bacteria would respond to the presence of Dermo by eliminating it, possibly through killing itself. Similar work has previously been done with bees and colony collapse.</p> | <p>One of the ultimate goals from using the downstream signaling approach is to develop a “Kamikaze Bacteria”. By this we hope that the bacteria would respond to the presence of Dermo by eliminating it, possibly through killing itself. Similar work has previously been done with bees and colony collapse.</p> | ||
<p>2). Microfluidics Approach- Size Based Cell sorter</p> | <p>2). Microfluidics Approach- Size Based Cell sorter</p> | ||
+ | <p style="text-align: center;"><img src="https://static.igem.org/mediawiki/parts/0/02/UMMicrofluidics.png" border="0" width="572" height="416" /></p> | ||
<p>Another avenue we plan to pursue is a microfluidics based detection approach. The overall concept would rely on the fact that E.coli cells are much smaller than Dermo cells. By creating a microfluidic device that sorts based on size, E.coli would travel into one output, whereas the Dermo and Dermo+E.coli complexes would travel into another output. Furthermore if the E.coli cells are fluorescently stained, analysis of the larger size fraction would indicate whether any E.coli cells are bound, confirming the presence of Dermo. While further differentiations based on size between bound and unbound Dermo may not be possible due to growth differences, this method will still suffice. </p> | <p>Another avenue we plan to pursue is a microfluidics based detection approach. The overall concept would rely on the fact that E.coli cells are much smaller than Dermo cells. By creating a microfluidic device that sorts based on size, E.coli would travel into one output, whereas the Dermo and Dermo+E.coli complexes would travel into another output. Furthermore if the E.coli cells are fluorescently stained, analysis of the larger size fraction would indicate whether any E.coli cells are bound, confirming the presence of Dermo. While further differentiations based on size between bound and unbound Dermo may not be possible due to growth differences, this method will still suffice. </p> | ||
<p>A microfluidic device would also contain the E.coli preventing them from entering the surrounding environment. Containment would be an obvious concern if a device like this were to ever hit the market. In this solution theoretically one could have pre-labeled E.coli in the machine stored and ready. The end user would simply intake a water sample right from the source, allow it to incubate, and process (sort) it. Collecting the larger fraction and viewing under a microscope would give a quick and easy real time method for detecting Dermo.</p> | <p>A microfluidic device would also contain the E.coli preventing them from entering the surrounding environment. Containment would be an obvious concern if a device like this were to ever hit the market. In this solution theoretically one could have pre-labeled E.coli in the machine stored and ready. The end user would simply intake a water sample right from the source, allow it to incubate, and process (sort) it. Collecting the larger fraction and viewing under a microscope would give a quick and easy real time method for detecting Dermo.</p> | ||
<p>We believe that with the groundwork we have laid this year these future endeavors will be made possible as UMaryland continues to grow and gain expertise.</p> | <p>We believe that with the groundwork we have laid this year these future endeavors will be made possible as UMaryland continues to grow and gain expertise.</p> | ||
- | <p> </p> | + | <p> </p> |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
</div> | </div> | ||
Line 172: | Line 131: | ||
<!-- end mid block div --> | <!-- end mid block div --> | ||
<!-- right block --> | <!-- right block --> | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
</div> | </div> | ||
<!-- end right block --> | <!-- end right block --> | ||
Line 184: | Line 140: | ||
<div class="yjsgspathway"> | <div class="yjsgspathway"> | ||
<ul class="breadcrumb "> | <ul class="breadcrumb "> | ||
- | <li class="active"><span class="divider"><span class="icon-yjsg-marker addtips" title="You are here: "></span></span></li><li itemscope itemtype="http://data-vocabulary.org/Breadcrumb"><a href="/ | + | <li class="active"><span class="divider"><span class="icon-yjsg-marker addtips" title="You are here: "></span></span></li><li itemscope itemtype="http://data-vocabulary.org/Breadcrumb"><a href="https://2014.igem.org/Team:UMaryland" class="pathway" itemprop="url"><span itemprop="title">Home</span></a><span class="icon-yjsg-pathway"></span></li><li itemscope itemtype="http://data-vocabulary.org/Breadcrumb"><a href="https://2014.igem.org/Team:UMaryland/project/overview" class="pathway" itemprop="url"><span itemprop="title">Project</span></a></li><li itemscope itemtype="http://data-vocabulary.org/Breadcrumb"><span class="icon-yjsg-pathway"></span><span itemprop="title">future applications</span></li></ul> |
</div> | </div> | ||
</div> | </div> | ||
Line 190: | Line 146: | ||
</div> | </div> | ||
<!-- end centerbottom--> | <!-- end centerbottom--> | ||
- | <div class="yjsg7_before"><div id="yjsg7" class="yjsg_grid yjsgsitew"><div id="user23" class="yjsgxhtml only_mod"><div class="yjsquare modid117"><div class="h2_holder"><h2 class="module_title"><span class="title_split titlesplit0">About</span> <span class="title_split titlesplit1">Umaryland</span></h2></div><div class="yjsquare_in"><p> | + | <div class="yjsg7_before"><div id="yjsg7" class="yjsg_grid yjsgsitew"><div id="user23" class="yjsgxhtml only_mod"><div class="yjsquare modid117"><div class="h2_holder"><h2 class="module_title"><span class="title_split titlesplit0">About</span> <span class="title_split titlesplit1">Umaryland</span></h2></div><div class="yjsquare_in"><p>UMaryland 2014 is the inaugural iGEM team of the University of Maryland, College Park. We are a combined effort of several departments and numerous faculty mentors. Although it is only our first year, we believe our hard work and dedication has paid off. We can't wait for this year's competition! GO TERPS!</p></div></div></div></div></div> <div class="footer_holders footer"><!-- footer --> |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
<!-- end footer --> | <!-- end footer --> | ||
- | <script type="text/javascript"> var logo_w = '240'; var site_w = '1200'; var site_f = '13px'; var sp=' | + | <script type="text/javascript"> var logo_w = '240'; var site_w = '1200'; var site_f = '13px'; var sp='https://2014.igem.org/Team:UMaryland'; var tp ='baseline'; var compileme =0; var fontc ='baseline_73331408039633'; var bootstrapv='bootstrap2'; var yver='2'; var yjsglegacy='1'; var yjsgrtl='2'; var menuanimation='fade';var menuanimationspeed=300; var YJSG_topmenu_font = '13px'; (function($){ $(window).load(function(){ $('.horiznav').SmoothDropJQ({ contpoz:0, horizLeftOffset: 20, horizRightOffset: -20, horizTopOffset: 20, verticalTopOffset:30, verticalLeftOffset: 10, maxOutside: 50 }); }) })(jQuery); </script> |
</div> <div id="mmenu_holder"> | </div> <div id="mmenu_holder"> | ||
<span class="yjmm_select" id="yjmm_selectid">future applications</span> | <span class="yjmm_select" id="yjmm_selectid">future applications</span> | ||
<select id="mmenu" class="yjstyled"> | <select id="mmenu" class="yjstyled"> | ||
- | <option value="/ | + | <option value="https://2014.igem.org/Team:UMaryland"> Homepage</option> |
<option value="https://2014.igem.org/Team:UMaryland/photos"> Photos</option> | <option value="https://2014.igem.org/Team:UMaryland/photos"> Photos</option> | ||
<option value="https://2014.igem.org/Team:UMaryland/meetourteam"> Team</option> | <option value="https://2014.igem.org/Team:UMaryland/meetourteam"> Team</option> |
Latest revision as of 19:43, 17 October 2014
About Umaryland
UMaryland 2014 is the inaugural iGEM team of the University of Maryland, College Park. We are a combined effort of several departments and numerous faculty mentors. Although it is only our first year, we believe our hard work and dedication has paid off. We can't wait for this year's competition! GO TERPS!