Team:Wageningen UR/overview/parts

From 2014.igem.org

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<h4><a href="#header">BioBricks</a></h4>
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<h3>Parts</h3><br/><br/>
+
 
 +
<h1>BioBricks</h1><br/>
 +
<p>In the table below, all new BioBricks can be found. Each BioBrick is sent in pSB1C3, unless mentioned otherwise. </p>
 +
<br/>
 +
 
 +
<figure>
 +
<figcaption>Table 1. BioBricks</figcaption>
<ul class="table">
<ul class="table">
<table>
<table>
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<th>Category</th>
<th>Category</th>
<th>BioBricks used</th>
<th>BioBricks used</th>
-
 
</tr>
</tr>
<tr>
<tr>
-
<td>BBa_K1493000</td>
+
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K1493000">BBa_K1493000</a> </td>
<td>FA regulation system</td>
<td>FA regulation system</td>
<td>Promoter only functional in presence of fusaric acid</td>
<td>Promoter only functional in presence of fusaric acid</td>
<td>Regulator</td>
<td>Regulator</td>
-
<td>-</td>
+
<td>-</td>
</tr>
</tr>
<tr>
<tr>
-
<td>BBa_K1493002</td>
+
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K1493002">BBa_K1493002</a> </td>
<td>FA regulation system</td>
<td>FA regulation system</td>
<td>Promoter only functional in presence of fusaric acid</td>
<td>Promoter only functional in presence of fusaric acid</td>
<td>Regulator</td>
<td>Regulator</td>
-
<td>-</td>
+
<td>-</td>
</tr>
</tr>
<tr>
<tr>
-
<td>BBa_K1493003</td>
+
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K1493003">BBa_K1493003</a><br><i>pSB1A2 instead of pSB1C3</i> </td>
<td>FA regulation system</td>
<td>FA regulation system</td>
-
<td>Promoter only functional in presence of fusaric acid, produces GFP when active</td>
+
<td>"Promoter only functional in presence of fusaric acid, produces GFP when active</td>
<td>Regulator</td>
<td>Regulator</td>
-
<td>Bba_I13504</td>
+
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_I13504">BBa_I13504</a> </td>
</tr>
</tr>
<tr>
<tr>
-
<td>BBa_K1493200</td>
+
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K1493200">BBa_K1493200</a> </td>
<td>PfrI</td>
<td>PfrI</td>
<td>Transcription factor involved in pyoverdine synethesis gene transcription</td>
<td>Transcription factor involved in pyoverdine synethesis gene transcription</td>
<td>Transcription factor</td>
<td>Transcription factor</td>
-
<td>Bba_B0034</td>
+
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a> </td>
</tr>
</tr>
<tr>
<tr>
-
<td>BBa_K1493300</td>
+
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K1493300">BBa_K1493300</a> </td>
<td>methionine-lyase</td>
<td>methionine-lyase</td>
<td>Converts L-methionine to methanethiol ammonium and 2-oxobutanoate</td>
<td>Converts L-methionine to methanethiol ammonium and 2-oxobutanoate</td>
-
<td>enzyme</td>
+
<td>Enzyme</td>
-
<td>-</td>
+
<td>-</td>
-
+
</tr>
</tr>
<tr>
<tr>
-
<td>BBa_K1493601</td>
+
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K1493501">BBa_K1493501</a> </td>
-
<td>LacI + Kis</td>
+
-
<td>Kid antitoxin</td>
+
-
<td>Composite</td>
+
-
<td>BBa_R0010</td>
+
-
</tr>
+
-
<tr>
+
-
<td>BBa_K1493602</td>
+
-
<td>LacI + Kid</td>
+
-
<td>Broad range toxin</td>
+
-
<td>Composite</td>
+
-
<td>BBa_J04500</td>
+
-
</tr>
+
-
<tr>
+
-
<td>BBa_K1493501</td>
+
<td>pRha+GFP</td>
<td>pRha+GFP</td>
<td>GFP controlled by pRha</td>
<td>GFP controlled by pRha</td>
<td>Reporter</td>
<td>Reporter</td>
-
<td>BBa_K914003 BBa_I13504</td>
+
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K914003">BBa_K914003</a> <a class="soft_link"  href="http://parts.igem.org/Part:BBa_I13504">BBa_I13504</a> </td>
</tr>
</tr>
<tr>
<tr>
-
<td>BBa_K1493502</td>
+
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K1493502">BBa_K1493502</a> </td>
-
<td>pcI/lac+GFP</td>
+
<td>pCI/lac+GFP</td>
-
<td>GFP controlled by pcI/lac</td>
+
<td>GFP controlled by pCI/lac</td>
<td>Reporter</td>
<td>Reporter</td>
-
<td>BBa_K909012 BBa_I13504</td>
+
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K909012">BBa_K909012</a> <a class="soft_link"  href="http://parts.igem.org/Part:BBa_I13504">BBa_I13504</a> </td>
</tr>
</tr>
<tr>
<tr>
-
<td>BBa_K1493503</td>
+
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K1493503">BBa_K1493503</a> </td>
-
<td>pcI/tet+GFP</td>
+
<td>pCI/tet+GFP</td>
-
<td>GFP controlled by pcI/tet</td>
+
<td>GFP controlled by pCI/tet</td>
<td>Reporter</td>
<td>Reporter</td>
-
<td>BBa_K909013 BBa_I13504</td>
+
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K909013">BBa_K909013</a> <a class="soft_link"  href="http://parts.igem.org/Part:BBa_I13504">BBa_I13504</a> </td>
</tr>
</tr>
<tr>
<tr>
-
<td>BBa_K1493504</td>
+
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K1493504">BBa_K1493504</a> </td>
<td>pTet+GFP</td>
<td>pTet+GFP</td>
<td>GFP controlled by pTet</td>
<td>GFP controlled by pTet</td>
<td>Reporter</td>
<td>Reporter</td>
-
<td>BBa_R0040 BBa_I13504</td>
+
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_R0040">BBa_R0040</a> <a class="soft_link"  href="http://parts.igem.org/Part:BBa_I13504">BBa_I13504</a> </td>
</tr>
</tr>
<tr>
<tr>
-
<td>BBa_K1493510</td>
+
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K1493510">BBa_K1493510</a> </td>
-
<td>pRHA+cI?</td>
+
<td>pRHA+CIλ</td>
-
<td>Repressor protein cI lambda controlled by pRha</td>
+
<td>Repressor protein CIλ controlled by pRha</td>
<td>Composite</td>
<td>Composite</td>
-
<td>BBa_K914003 BBa_P0451</td>
+
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K914003">BBa_K914003</a> <a class="soft_link"  href="http://parts.igem.org/Part:BBa_P0451">BBa_P0451</a> </td>
</tr>
</tr>
<tr>
<tr>
-
<td>BBa_K1493520</td>
+
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K1493520">BBa_K1493520</a> </td>
<td>pRHA+LacI</td>
<td>pRHA+LacI</td>
<td>Repressor protein LacI controlled by pRha</td>
<td>Repressor protein LacI controlled by pRha</td>
<td>Composite</td>
<td>Composite</td>
-
<td>BBa_K914003 BBa_P0412</td>
+
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K914003">BBa_K914003</a> <a class="soft_link"  href="http://parts.igem.org/Part:BBa_P0412">BBa_P0412</a> </td>
</tr>
</tr>
<tr>
<tr>
-
<td>BBa_K1493530</td>
+
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K1493530">BBa_K1493530</a> </td>
<td>pRHA+TetR</td>
<td>pRHA+TetR</td>
<td>Repressor protein TetR controlled by pRha</td>
<td>Repressor protein TetR controlled by pRha</td>
<td>Composite</td>
<td>Composite</td>
-
<td>BBa_K914003 BBa_P0440</td>
+
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K914003">BBa_K914003</a> <a class="soft_link"  href="http://parts.igem.org/Part:BBa_P0440">BBa_P0440</a> </td>
</tr>
</tr>
<tr>
<tr>
-
<td>BBa_K1493540</td>
+
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K1493540">BBa_K1493540</a> </td>
-
<td>pRHA+cI?+LacI</td>
+
<td>pRHA+CIλ +LacI</td>
-
<td>Repressor proteins cI lambda and LacI controlled by pRha</td>
+
<td>Repressor proteins CIλ and LacI controlled by pRha</td>
<td>Composite</td>
<td>Composite</td>
-
<td>BBa_K914003 BBa_S0103 BBa_P0412</td>
+
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K914003">BBa_K914003</a> <a class="soft_link"  href="http://parts.igem.org/Part:BBa_S0103">BBa_S0103</a> <a class="soft_link"  href="http://parts.igem.org/Part:BBa_P0412">BBa_P0412</a> </td>
</tr>
</tr>
<tr>
<tr>
-
<td>BBa_K1493550</td>
+
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K1493550">BBa_K1493550</a> </td>
-
<td>pRHA+cI?+TetR</td>
+
<td>pRHA+CI labda+TetR</td>
-
<td>Repressor proteins cI lambda and TetR controlled by pRha</td>
+
<td>Repressor proteins CIλ and TetR controlled by pRha</td>
<td>Composite</td>
<td>Composite</td>
-
<td>BBa_K914003 BBa_I13504</td>
+
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K914003">BBa_K914003</a> <a class="soft_link"  href="http://parts.igem.org/Part:BBa_I13504">BBa_I13504</a> </td>
</tr>
</tr>
<tr>
<tr>
-
<td>BBa_K1493560</td>
+
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K1493560">BBa_K1493560</a> </td>
-
<td>pRHA+cI?+pcI/tet+GFP</td>
+
<td>pRHA+CIλ+pcI/tet+GFP</td>
<td>Kill Switch Input/output</td>
<td>Kill Switch Input/output</td>
<td>Reporter</td>
<td>Reporter</td>
-
<td>BBa_K914003 BBa_P0451 BBa_K909013 BBa_I13504</td>
+
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K914003">BBa_K914003</a> <a class="soft_link"  href="http://parts.igem.org/Part:BBa_P0451">BBa_P0451</a> <a class="soft_link"  href="http://parts.igem.org/Part:BBa_K909013">BBa_K909013</a> <a class="soft_link"  href="http://parts.igem.org/Part:BBa_I13504">BBa_I13504</a> </td>
 +
</tr>
 +
<tr>
 +
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K1493601">BBa_K1493601</a> </td>
 +
<td>LacI + Kis</td>
 +
<td>Kis antitoxin</td>
 +
<td>Composite</td>
 +
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_R0010">BBa_R0010</a> </td>
 +
</tr>
 +
<tr>
 +
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K1493602">BBa_K1493602</a> </td>
 +
<td>LacI + Kis</td>
 +
<td>LacI promoter + Kis antitoxin</td>
 +
<td>Composite</td>
 +
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_J04500">BBa_J04500</a> </td>
 +
</tr>
 +
<tr>
 +
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K1493603">BBa_K1493603</a> </td>
 +
<td>Epsilon + lacI</td>
 +
<td>Zeta antitoxin</td>
 +
<td>Composite</td>
 +
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_R0010">BBa_R0010</a> </td>
 +
</tr>
 +
<tr>
 +
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K1493701">BBa_K1493701</a> </td>
 +
<td>pCI/lac+TetR+GFP</td>
 +
<td>Part of toggle switch with reporter</td>
 +
<td>Composite</td>
 +
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K909012">BBa_K909012</a> <a class="soft_link"  href="http://parts.igem.org/Part:BBa_S03518">BBa_S03518</a> <a class="soft_link"  href="http://parts.igem.org/Part:BBa_I13504">BBa_I13504</a> </td>
 +
</tr>
 +
<tr>
 +
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K1493702">BBa_K1493702</a> </td>
 +
<td>pCI/lac+TetR</td>
 +
<td>Part of toggle switch</td>
 +
<td>Composite</td>
 +
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K909012">BBa_K909012</a> <a class="soft_link"  href="http://parts.igem.org/Part:BBa_P0440">BBa_P0440</a> </td>
 +
</tr>
 +
<tr>
 +
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K1493703">BBa_K1493703</a> </td>
 +
<td>ptet+LacI+GFP</td>
 +
<td>Part of Toggle switch with reporter</td>
 +
<td>Composite</td>
 +
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_R0040">BBa_R0040</a> <a class="soft_link"  href="http://parts.igem.org/Part:BBa_S0100">BBa_S0100</a> <a class="soft_link"  href="http://parts.igem.org/Part:BBa_I13504">BBa_I13504</a> </td>
 +
</tr>
 +
<tr>
 +
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K1493801">BBa_K1493801</a> </td>
 +
<td>Promoter with Tet operator site</td>
 +
<td>Promoter repressed by TetR</td>
 +
<td>Regulatory</td>
 +
<td>-</td>
 +
</tr>
 +
<tr>
 +
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K1493802">BBa_K1493802</a> </td>
 +
<td>Promoter with Tet operator site</td>
 +
<td>Promoter repressed by TetR</td>
 +
<td>Regulatory</td>
 +
<td>-</td>
 +
</tr>
 +
<tr>
 +
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K1493803">BBa_K1493803</a> </td>
 +
<td>promoter with CIλ and LacI operator sites</td>
 +
<td>Promoter repressed by CIλ and LacI</td>
 +
<td>Regulatory</td>
 +
<td>-</td>
 +
</tr>
 +
<tr>
 +
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K1493804">BBa_K1493804</a> </td>
 +
<td>promoter with CIλ and Tet operator sites</td>
 +
<td>Promoter repressed by CIλ and TetR</td>
 +
<td>Regulatory</td>
 +
<td>-</td>
 +
</tr>
 +
<tr>
 +
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K1493805">BBa_K1493805</a> </td>
 +
<td>promoter with CIλ and Tet operator sites</td>
 +
<td>Promoter repressed by CIλ and TetR</td>
 +
<td>Regulatory</td>
 +
<td>-</td>
 +
</tr>
 +
<tr>
 +
<td><a class="soft_link"  href="http://parts.igem.org/Part:BBa_K1493806">BBa_K1493806</a> </td>
 +
<td>promoter with CIλ and LacI operator sites</td>
 +
<td>Promoter repressed by CIλ and LacI</td>
 +
<td>Regulatory</td>
 +
<td>-</td>
</tr>
</tr>
</table>
</table>
 +
  </ul>
  </ul>
 +
</figure>
 +
<br>
 +
<p style="float:right"><b>Continue to <a href="https://2014.igem.org/Team:Wageningen_UR/project/fungal_sensing"  class="soft_link">Fungal Sensing >></a> </b>
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Latest revision as of 02:40, 18 October 2014

Wageningen UR iGEM 2014

 

 

BioBricks


In the table below, all new BioBricks can be found. Each BioBrick is sent in pSB1C3, unless mentioned otherwise.


Table 1. BioBricks

Continue to Fungal Sensing >>