Team:Tsinghua-A

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            <h1>Welcome to Tsinghua-A Team Wiki</h1>
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            <h2> To TALE a new world.  <a href="https://2014.igem.org/Team:Tsinghua-A/Project"><i class="icon-chevron-right"></i> See Project</a></h2>
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            <h1>Welcome to Tsinghua-A Team Wiki</h1>
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            <h2> Coding to reveal the code.  <a href="https://2014.igem.org/Team:Tsinghua-A/Modeling"><i class="icon-chevron-right"></i> See Modeling</a> </h2>
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            <h1>Welcome to Tsinghua-A Team Wiki</h1>
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            <h2> Practice humanity with technology. <a href="https://2014.igem.org/Team:Tsinghua-A/HumanPractice"><i class="icon-chevron-right"></i> See Human Practice</a> </h2>
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        <h2> Introduction </h2>
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          <h3>Transactivator-Like Effectors (TALEs) are a technology that once revolutionized the way researchers manipulate DNA with exceptional site specificity.Though it works well in eukaryotic organisms, it is not available in prokaryotic organisms. We come up with the idea that the long repeating sequence of the TALEs coding sequence leads to it. In our project, we attempt to construct a marvelous TALEs system which can efficiently work in prokaryotic organisms. By optimizing the TALEs coding sequence with modeling methods and testing it by Golden Gate Assembly and Report System, we hope we can have a handle tool for gene editing in prokaryotic organisms.
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          <a href="https://2014.igem.org/Team:Tsinghua-A/Project">
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            <h2>Project</h2>
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            <p>Background, hypothesis and workflow of designer TALE. </p>
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            <h2>Modeling</h2>
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            <p>Optimization of TALE sequence and simulation of the gene circuit. </p>
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            <h2>Human Practice</h2>
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            <p>Lectures, seminar, questionnaires and raising ethic problems.  </p>
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            <h2>Team</h2>
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            <p>Department of Automatics, Life Science and Medical School. </p>
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          <h2> Acknowledgements </h2>
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                    <img src="https://static.igem.org/mediawiki/2014/4/45/Tsinghua-A_acc.png" alt="Title">
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-
</div>
+
-
 
+
-
<div id="containerPlaceholder" class="containerPlaceholder" ></div>
+
-
+
-
</div>
+
-
 
+
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<div id="containerMiddleCenterBottom" class="containerMiddleCenterBottom">
+
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</div>
+
-
 
+
-
</td>
+
-
 
+
-
<td id="containerMiddleRight" class="containerMiddleRight"></td>
+
-
</tr>
+
-
</table>
+
-
+
-
<table class="containerBottom" cellpadding='0' cellspacing='0'>
+
-
<tr valign='top'>
+
-
<td class="left"></td>
+
-
<td class="center"></td>
+
-
<td class="right"></td>
+
-
</tr>
+
-
</table>
+
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</div>
+
-
 
+
-
</div>
+
-
</td>
+
-
</tr>
+
-
</table>
+
-
 
+
-
<table class="absBottomTable" cellpadding="0" cellspacing="0">
+
-
<tr>
+
-
<td align="center">
+
-
<div id="absBottomForms" class="forms sideForms absForms">
+
-
<div style="position:absolute;"></div><!-- for ie6 -->
+
-
+
-
</div>
+
-
</td>
+
-
</tr>
+
-
</table>
+
-
 
+
-
<table id="webFooterTable" class="webFooterTable" cellpadding="0" cellspacing="0">
+
-
<tr>
+
-
<td align="center" valign="top">
+
-
<div id="webFooter" class="webFooter">
+
-
+
-
 
+
-
 
+
-
 
+
-
<div id="footer" class="footer" >
+
-
<table class="footerTop" cellpadding="0" cellspacing="0">
+
-
<tr valign="top">
+
-
<td class="topLeft"></td>
+
-
<td class="topCenter"></td>
+
-
<td class="topRight"></td>
+
-
</tr>
+
-
</table>
+
-
<table class="footerMiddle" cellpadding="0" cellspacing="0">
+
-
<tr valign="top">
+
-
<td class="middleLeft"></td>
+
-
<td class="middleCenter" align="center">
+
-
<div class="footerContent">
+
-
 
+
-
<div id="footerNav" class="footerNav">
+
-
<span class='footerNavItem' id='footer2'><a hidefocus='true' href='/'>首页</a></span>
+
-
<span class='footerSep'>|</span>
+
-
<span class='footerNavItem' id='footer101'><a hidefocus='true' href='/col.jsp?id=101'>关于我们</a></span>
+
-
<span class='footerSep'>|</span>
+
-
<span class='footerNavItem' id='footer102'><a hidefocus='true' href='/col.jsp?id=102'>联系我们</a></span>
+
-
 
+
-
</div>
+
-
 
+
-
<div class="footerInfo">
+
-
<font face='Arial'>©</font>2014&nbsp;suneamon&nbsp;版权所有
+
-
</div>
+
-
 
+
-
<div class="footerSupport">
+
-
 
+
-
<span class='footerFaisco'>技术支持:<a hidefocus='true' href='http://www.faisco.com/?_ta=4' onclick='Site.logDog(100065, 0);' target='_blank'>凡科网</a></span>
+
-
<span class='footerSep'>|</span>
+
-
<span class='footerMobile'><a hidefocus='true' href='http://m.suneamon.icoc.cc' target='_blank'>手机版</a></span>
+
-
<span class='footerSep'>|</span>
+
-
<span id='footerLogin' class='footerLogin'><a name='popupLogin' hidefocus='true' href='javascript:;' onclick='Site.popupLogin("http://www.faisco.cn",4438089);Fai.closeTip("#footerLogin");return false;'>管理登录</a></span>
+
-
 
+
-
<span class="bgplayerButton" id="bgplayerButton" style="display:none;"></span>
+
-
</div>
+
-
+
-
+
-
+
-
</div>
+
-
</td>
+
-
<td class="middleRight"></td>
+
-
</tr>
+
-
</table>
+
-
<table class="footerBottom" cellpadding='0' cellspacing='0'>
+
-
<tr valign='top'>
+
-
<td class="bottomLeft"></td>
+
-
<td class="bottomCenter"></td>
+
-
<td class="bottomRight"></td>
+
-
</tr>
+
-
</table>
+
-
 
+
-
</div>
+
-
 
+
-
+
-
</div>
+
-
</td>
+
-
</tr>
+
-
</table>
+
-
 
+
-
<div class="clearfloat"></div>
+
-
</div>
+
-
</div>
+
-
 
+
-
<!-- 左下角广告-->
+
-
+
-
<div class='siteAdvertisement_box' style="display:none;">
+
-
<div class='siteAdvertisement_Inner'>
+
-
<div class='siteAdvertisement_title'>
+
-
<a class='reportUrl' href=http://www.faisco.com/ts.html?t=3&a=suneamon  target='_blank' >举报</a>
+
-
<a class='closeImg' href='javascript:void(0)'></a>
+
-
</div>
+
-
+
-
 
+
-
<a class="siteAdvertisement_adImg" target='_blank' href=http://www.faisco.com/?_ta=4 ><img src="http://0.ss.faidns.com/image/site/faiscoAd/faisco_ad.jpg" width="125" height="125" alt="轻松建网站" /></a>
+
-
</div>
+
-
<a  class='freeJZ'  href=http://www.faisco.com/?_ta=4  target='_blank'><span>免费建站</span></a>
+
-
 
+
-
</div>
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
<div class="floatLeftTop">
+
-
<div id="floatLeftTopForms" class="forms sideForms floatForms">
+
-
+
-
</div>
+
-
</div>
+
-
<div class="floatRightTop">
+
-
<div id="floatRightTopForms" class="forms sideForms floatForms">
+
-
<div id='module37' _indexClass='formIndex1' class='form  formIndex1 formStyle67' title='' style='position:absolute;top:180px;left:0px;width:180px;' _side='1' _inTab='0' _inMulMCol='0' _autoHeight='1' _global='true' _independent='false' >
+
-
<table class='formTop formTop37' cellpadding='0' cellspacing='0'><tr><td class='left'></td><td class='center'></td><td class='right'></td></tr></table>
+
-
<table class='formBanner formBanner37' cellpadding='0' cellspacing='0'><tr>
+
-
<td class='left left37'></td>
+
-
<td class='center center37' valign='top'>
+
-
<table cellpadding='0' cellspacing='0' class='formBannerTitle formBannerTitle37'><tr>
+
-
<td class='titleLeft titleLeft37' valign='top'>
+
-
</td>
+
-
<td class='titleCenter titleCenter37' valign='top'>
+
-
<div class='titleText titleText37'><span>
+
-
分享网站</span></div>
+
-
</td>
+
-
<td class='titleRight titleRight37' valign='top'>
+
-
</td>
+
-
</tr></table>
+
-
<div class='formBannerOther formBannerOther37'>
+
-
<div class='formBannerBtn formBannerBtn37'>
+
-
<a hidefocus='true' href='javascript:;' onclick='Site.closeAd("module37");return false;' class='g_close'><span style='_display:none;' >&nbsp;</span></a>
+
-
</div>
+
-
</div>
+
-
</td>
+
-
<td class='right right37'></td>
+
-
</tr></table>
+
-
<table class='formMiddle formMiddle37' cellpadding='0' cellspacing='0'><tr>
+
-
<td class='formMiddleLeft formMiddleLeft37'></td>
+
-
<td class='formMiddleCenter formMiddleCenter37' valign='top'>
+
-
<div class='formMiddleContent formMiddleContent37  '>
+
-
<div class='shareInfo'><div class='shareCtrl shareNotTitlePanel shareMt'><a hidefocus='true' title='分享到腾讯微博'  href='javascript:;' onclick='window.open("http://v.t.qq.com/share/share.php?title=%E3%80%90suneamon%E3%80%91http%3A%2F%2Fwww.suneamon.icoc.cc%2F%3F_sc%3D1&url=http://www.suneamon.icoc.cc/?_sc=1&pic=")'><div class='shareIcon qq_weibo'></div><div class='shareCtrl'>腾讯微博</div></a><a hidefocus='true' title='分享到复制网址'  href='javascript:;'  onclick="Site.copyWebSite('http://www.suneamon.icoc.cc')"><div class='shareIcon copy'></div><div class='shareCtrl'>复制网址</div></a><a hidefocus='true' title='分享到新浪微博'  href='javascript:;' onclick='window.open("http://service.weibo.com/share/share.php?title=%E3%80%90suneamon%E3%80%91http%3A%2F%2Fwww.suneamon.icoc.cc%2F%3F_sc%3D1&url=http://www.suneamon.icoc.cc/?_sc=1&pic=")'><div class='shareIcon sina_weibo'></div><div class='shareCtrl'>新浪微博</div></a><a hidefocus='true' title='分享到QQ空间'  href='javascript:;' onclick='window.open("http://sns.qzone.qq.com/cgi-bin/qzshare/cgi_qzshare_onekey?summary=http%3A%2F%2Fwww.suneamon.icoc.cc%2F%3F_sc%3D1&url=http://www.suneamon.icoc.cc/?_sc=1&pics=&title=%E3%80%90suneamon%E3%80%91")'><div class='shareIcon qq_zone'></div><div class='shareCtrl'>QQ空间</div></a><a hidefocus='true' title='分享到开心网'  href='javascript:;' onclick='window.open("http://www.kaixin001.com/rest/records.php?content=%E3%80%90suneamon%E3%80%91http%3A%2F%2Fwww.suneamon.icoc.cc%2F%3F_sc%3D1&style=11")'><div class='shareIcon kaixin001'></div><div class='shareCtrl'>开心网</div></a><a hidefocus='true' title='分享到人人网'  href='javascript:;' onclick='window.open("http://widget.renren.com/dialog/share?resourceUrl=http://www.suneamon.icoc.cc/?_sc=1&description=http%3A%2F%2Fwww.suneamon.icoc.cc%2F%3F_sc%3D1&title=%E3%80%90suneamon%E3%80%91&pic=")'><div class='shareIcon renren'></div><div class='shareCtrl'>人人网</div></a><a hidefocus='true' title='分享到豆瓣网'  href='javascript:;' onclick='window.open("http://shuo.douban.com/!service/share?name=%E3%80%90suneamon%E3%80%91http%3A%2F%2Fwww.suneamon.icoc.cc%2F%3F_sc%3D1&text=http://www.suneamon.icoc.cc/?_sc=1&image=")'><div class='shareIcon douban'></div><div class='shareCtrl'>豆瓣网</div></a><a hidefocus='true' title='分享到淘江湖'  href='javascript:;' onclick='window.open("http://share.jianghu.taobao.com/share/addShare.htm?url=http://www.suneamon.icoc.cc/?_sc=1&title=%E3%80%90suneamon%E3%80%91")'><div class='shareIcon taojianghu'></div><div class='shareCtrl'>淘江湖</div></a><a hidefocus='true' title='分享到搜狐微博'  href='javascript:;' onclick='window.open("http://t.sohu.com/third/post.jsp?url=http%3A%2F%2Fwww.suneamon.icoc.cc%2F%3F_sc%3D1&title=%A1%BEsuneamon%A1%BF")'><div class='shareIcon sohu_weibo'></div><div class='shareCtrl'>搜狐微博</div></a><a hidefocus='true' title='分享到网易微博'  href='javascript:;' onclick='window.open("http://t.163.com/article/user/checkLogin.do?info=%E3%80%90suneamon%E3%80%91http%3A%2F%2Fwww.suneamon.icoc.cc%2F%3F_sc%3D1&images=&togImg=true")'><div class='shareIcon netease_weibo'></div><div class='shareCtrl'>网易微博</div></a><a hidefocus='true' title='分享到百度空间'  href='javascript:;' onclick='window.open("http://apps.hi.baidu.com/share/?content=http%3A%2F%2Fwww.suneamon.icoc.cc%2F%3F_sc%3D1&url=http://www.suneamon.icoc.cc/?_sc=1&title=%E3%80%90suneamon%E3%80%91")'><div class='shareIcon baidu_hi'></div><div class='shareCtrl'>百度空间</div></a><a hidefocus='true' title='分享到百度贴吧'  href='javascript:;' onclick='window.open("http://tieba.baidu.com/i/app/open_share_api?comment=&pic=&url=http://www.suneamon.icoc.cc/?_sc=1&title=%E3%80%90suneamon%E3%80%91http%3A%2F%2Fwww.suneamon.icoc.cc%2F%3F_sc%3D1")'><div class='shareIcon baidu_tieba'></div><div class='shareCtrl'>百度贴吧</div></a></div><div class='clearfloat'></div></div></div>
+
-
</td>
+
-
<td class='formMiddleRight formMiddleRight37'></td>
+
-
</tr></table>
+
-
<table class='formBottom formBottom37' cellpadding='0' cellspacing='0'><tr><td class='left left37'></td><td class='center center37'></td><td class='right right37'></td></tr></table>
+
-
<div class='clearfloat clearfloat37'></div>
+
-
</div>
+
-
 
+
-
 
+
-
</div>
+
-
</div>
+
-
<div class="floatLeftBottom">
+
-
<div id="floatLeftBottomForms" class="forms sideForms floatForms">
+
-
+
-
</div>
+
-
</div>
+
-
<div class="floatRightBottom">
+
-
<div id="floatRightBottomForms" class="forms sideForms floatForms">
+
-
+
-
</div>
+
-
</div>
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
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-
 
+
-
<div id="bgMusic"  class="bgMusic">
+
-
+
-
</div>
+
-
+
-
 
+
-
 
+
-
<script type="text/javascript" src="http://0.ss.faidns.com/js/comm/jquery/jquery-core.min.js?v=201404111745"></script>
+
-
<script type="text/javascript" src="http://0.ss.faidns.com/js/comm/jquery/jquery-mousewheel.min.js?v=201408111734"></script>
+
-
<script type="text/javascript" src="http://0.ss.faidns.com/js/comm/fai.min.js?v=201409221731"></script>
+
-
<script type="text/javascript" src="http://0.ss.faidns.com/js/comm/jquery/jquery-ui-core.min.js?v=201409151747"></script>
+
-
<script type="text/javascript" src="http://0.ss.faidns.com/js/site.min.js?v=201410141438"></script>
+
-
<script type="text/javascript" src="http://0.ss.faidns.com/js/locale/2052.min.js?v=201407141810"></script>
+
-
<script type="text/javascript" src="http://0.ss.faidns.com/js/comm/ZeroClipboard/ZeroClipboard.min.js?v=201212061014"></script>
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
<script type="text/javascript">
+
-
Fai.top = window;
+
-
var bgmCloseToOpen = false;
+
-
window.onerror = function() {
+
-
if (typeof Site == 'undefined') {
+
-
alert('您的网页未加载完成,请尝试按“CTRL+功能键F5”重新加载。');
+
-
}
+
-
};
+
-
var _colOtherStyleData = {"independentList":[],"y":0,"h":0,"layout4Width":0,"layout5Width":0}; // 当前页面的数据   
+
-
var _templateOtherStyleData = {"h":584,"independentList":[],"y":0,"layout4Width":0,"layout5Width":0}; // 全局的数据 
+
-
$(function() {
+
-
if(false){
+
-
Fai.ing("",true);
+
-
}
+
-
//topBarMember
+
-
+
-
+
-
// 管理态下, QQ/微博登陆 禁止登陆
+
-
if( _manageMode ) {
+
-
$('#memberBar .l_Btn').click(function(){
+
-
Fai.ing('您目前处于网站管理状态,请先点击网站右上方的“退出”后再登录会员。', true);
+
-
});
+
-
//绑定放大镜遮罩效果事件
+
-
Site.bindEventToOverLayer();
+
-
}
+
-
+
-
+
-
+
-
+
-
var faiscoAd = $.cookie('faiscoAd',{path:'/'});
+
-
+
-
if( true && faiscoAd !== "false" ){
+
-
$(".siteAdvertisement_box").show();
+
-
}
+
-
+
-
+
-
 
+
-
+
-
+
-
+
-
+
-
// 绑定退出事件
+
-
$(window).bind("beforeunload", function(e) {
+
-
 
+
-
+
-
if(bgmCloseToOpen){
+
-
Site.bgmFlushContinue();
+
-
}
+
-
+
-
+
-
+
-
+
-
+
-
});
+
-
+
-
 
+
-
Site.initTemplateLayout(1, true);
+
-
 
+
-
// spider统计
+
-
+
-
 
+
-
+
-
// ajax统计
+
-
Site.total({colId:2, pdId:-1, ndId:-1, sc:0, rf:"http://suneamon.faisco.cn/index.jsp"});
+
-
//保留旧用户的初始化版式区域4 和区域5 中,区域4的padding-right空间
+
-
Site.colLayout45Width();
+
-
 
+
-
Site.initBanner({"_open":true,"data":[{"title":"","desc":"","imgWidth":1440,"imgHeight":380,"src":"http://0.ss.faidns.com/image/template/banners/1831.jpg"}],"width":1440,"height":380,"playTime":4000,"animateTime":1500,"from":"banner","btnType":1}, {"_open":true,"type":64,"position":0,"positionTop":0,"positionLeft":0,"text1":"自定义文字1","text2":"自定义文字2","style1":"宋体","style2":"宋体","color1":"#000","color2":"#FFFFFF","size1":"50","size2":"30"}, 4);
+
-
Site.loadProductTile(306, false, true, -1);
+
-
 
+
-
Site.loadNewsList(316);
+
-
 
+
-
 
+
-
 
+
-
 
+
-
+
-
 
+
-
 
+
-
Site.initPage(); // 这个要放在最后,因为模块组初始化时会把一些模块隐藏,导致没有高度,所以要放最后执行
+
-
 
+
-
+
-
+
-
+
-
setTimeout("afterModuleLoaded()", 0);
+
-
 
+
-
if( _manageMode ) {
+
-
+
-
}
+
-
+
-
if (!_oem && _manageMode) {
+
-
Site.siteGuideInit();
+
-
if( false ){
+
-
$.cookie('_loadedRegStatIframe',true,{ expires: 2 });
+
-
}
+
-
}
+
-
+
-
});
+
-
 
+
-
function afterModuleLoaded() {
+
-
Site.initPage2();
+
-
 
+
-
 
+
-
} // afterModuleLoaded end
+
-
 
+
-
var _portalHost = 'www.faisco.cn';
+
-
 
+
-
var _lcid = 2052;
+
-
var _userHostName = 'www.suneamon.icoc.cc';
+
-
var _siteDomain = 'http://www.suneamon.icoc.cc';
+
-
var _signupDays = 0;
+
-
var _cid = 4438089;
+
-
var _resRoot = 'http://0.ss.faidns.com';
+
-
var _colId = 2;
+
-
var _extId = 0;
+
-
var _fromColId = -1;
+
-
var _designAuth = false;
+
-
var _manageMode = false;
+
-
var _oem = false;
+
-
var _siteAuth = 0;
+
-
var _adm = false;
+
-
var _siteVer = 10;
+
-
var _manageStatus = false;
+
-
 
+
-
var nav2SubMenu=[];
+
-
var nav12SubMenu=[];
+
-
var nav21SubMenu=[];
+
-
var nav9SubMenu=[];
+
-
var nav101SubMenu=[];
+
-
var nav102SubMenu=[];
+
-
 
+
-
var _customBackgroundData = {"styleDefault":true,"s":true,"h":false,"r":3,"o":"","sw":-1,"e":0,"wbh":-1,"wbw":-1,"clw":-1,"crw":-1,"id":"","p":"","bBg":{"y":0,"r":3,"f":"","p":"","c":"#000"},"cBg":{"y":0,"r":3,"f":"","p":"","c":"#000"},"cmBg":{"y":0,"r":3,"f":"","p":"","c":"#000"}};          //自定义的数据
+
-
var _templateBackgroundData = {"styleDefault":true,"s":true,"h":false,"r":3,"o":"","sw":-1,"e":0,"wbh":-1,"wbw":-1,"clw":-1,"crw":-1,"id":"","p":"","bBg":{"y":0,"r":3,"f":"","p":"","c":"#000"},"cBg":{"y":0,"r":3,"f":"","p":"","c":"#000"},"cmBg":{"y":0,"r":3,"f":"","p":"","c":"#000"}};// 模版的数据
+
-
var _useTemplateBanner = true; // 是否使用全局模版
+
-
var _templateBannerData = {"ce":{"c1":"#000","c2":"#FFFFFF"},"pl":0,"n":[{"t":0,"i":"1831","e":0,"u":"","p":"http://0.ss.faidns.com/image/template/banners/1831.jpg","w":1440,"h":380}],"s":4,"o":true,"t":64,"i":4000,"a":1500,"h":false,"p":0,"pt":0,"bt":1,"l":[],"f":{},"c":3}; // 模版的数据
+
-
var _pageBannerData = {"s":0,"i":4000,"a":1500,"h":false,"o":false,"t":1,"p":0,"pt":0,"pl":0,"bt":1,"l":[],"f":{},"ce":{},"n":[],"c":3}; // 当前页面的自定义数据(页面独立样式设置)
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Latest revision as of 03:01, 18 October 2014

Introduction

Transactivator-Like Effectors (TALEs) are a technology that once revolutionized the way researchers manipulate DNA with exceptional site specificity.Though it works well in eukaryotic organisms, it is not available in prokaryotic organisms. We come up with the idea that the long repeating sequence of the TALEs coding sequence leads to it. In our project, we attempt to construct a marvelous TALEs system which can efficiently work in prokaryotic organisms. By optimizing the TALEs coding sequence with modeling methods and testing it by Golden Gate Assembly and Report System, we hope we can have a handle tool for gene editing in prokaryotic organisms.

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Acknowledgements

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