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Team:Hannover/Background Arabidopsis
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| - | <h1>Background / The Use of <i>Arabidopsis thaliana</i></h1> | + | <h1><a href="https://2014.igem.org/Team:Hannover/Background_Project">Background</a> / The Use of <i>Arabidopsis thaliana</i></h1> |
| - | <p class="text"> | + | <p class="text">Our aim was to be able to use the protein in terrestrial (<i>Arabidopsis thaliana</i>) as well as in aquatic plants (<i>Wolffia australiana</i>/Duckweed). Since no-one has had much experience with the transformation of <i>Wolffia</i>, we decided to demonstrate the principle initially in the model organisms <i>A. thaliana</i> and <i>Nicotiana tabacum</i>. After we integrated our T4MBP in the pORE_E3 expression vector, which can be used in plants, <i>Rhizobium radiobacter</i> (<i>R. radiobacter</i>) was transformed.<br><br> |
| - | <i>Arabidopsis thaliana</i> (<i>A. thaliana</i>) is the most important model organism in plant science. It is easy to transform via <i>R. radiobacter</i> and has a <span id='a1'>short regeneration time</span>. For the transformation of <i>A. thaliana</i> we chose the floral dip method. Therefore plants with inflorescence were required. Also a liquid culture of the T4MBP-carrying <i>R. radiobacter</i> was obligatory. This form of transformation is very simple, because we just had to dip the plants with the inflorescence into an infiltration medium, which contains <i>R. radiobacter</i>. After three weeks we collected | + | <i>Arabidopsis thaliana</i> (<i>A. thaliana</i>) is the most important model organism in plant science. It is easy to transform via <i>R. radiobacter</i> and has a <span id='a1'>short regeneration time</span>. For the transformation of <i>A. thaliana</i> we chose the floral dip method. Therefore plants with inflorescence were required. Also a liquid culture of the T4MBP-carrying <i>R. radiobacter</i> was obligatory. This form of transformation is very simple, because we just had to dip the plants with the inflorescence into an infiltration medium, which contains <i>R. radiobacter</i>. After three weeks the plants were withered an we collected their seeds. To select the transgenic seeds all seeds were construed on selection medium.<br><br>In the future we want to raise the transgenic seeds and plant <i>A. thaliana</i> on heavy metal containing medium to proof the function of our T4MBP.</p> |
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| - | <table> | + | <tr><td><a href="https://static.igem.org/mediawiki/2014/4/43/Hannover_20141003_Arabidopsis1.jpg |
| - | <tr><td | + | " data-lightbox="galery" data-title="<i>A. thaliana</i>"><img src="https://static.igem.org/mediawiki/2014/4/43/Hannover_20141003_Arabidopsis1.jpg" width="180px" style="center"></a></td><td><a href="https://static.igem.org/mediawiki/2014/0/02/Hannover_20141004_2.jpg |
| - | " data-lightbox="galery" data-title=""><img src="https://static.igem.org/mediawiki/2014/4/43/Hannover_20141003_Arabidopsis1.jpg" width=" | + | " data-lightbox="galery" data-title="<i>A. thaliana</i> 6 weeks old"><img src="https://static.igem.org/mediawiki/2014/0/02/Hannover_20141004_2.jpg |
| - | " data-lightbox="galery" data-title=""><img src="https://static.igem.org/mediawiki/2014/0/02/Hannover_20141004_2.jpg | + | " width="180px" style="center"></a></td><td><a href="https://static.igem.org/mediawiki/2014/7/75/Hannover_20141015_Floral_dip2.jpg |
| - | " width=" | + | " data-lightbox="galery" data-title="Floral dip with <i>A. thaliana</i>"><img src="https://static.igem.org/mediawiki/2014/thumb/7/75/Hannover_20141015_Floral_dip2.jpg/99px-Hannover_20141015_Floral_dip2.jpg |
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| - | <div class='annotation' ref='a1'>Although Arabidopsis thaliana is easy and quick to regenerate | + | <div class='annotation' ref='a1'>Although <i>Arabidopsis thaliana</i> is, for a plant, easy and quick to regenerate, it still takes way more time (about 6 weeks) than the transformation of bacteria. So you will need a good timetable while working with plants!</div> |
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Latest revision as of 13:28, 17 October 2014
Background / The Use of Arabidopsis thaliana
Our aim was to be able to use the protein in terrestrial (Arabidopsis thaliana) as well as in aquatic plants (Wolffia australiana/Duckweed). Since no-one has had much experience with the transformation of Wolffia, we decided to demonstrate the principle initially in the model organisms A. thaliana and Nicotiana tabacum. After we integrated our T4MBP in the pORE_E3 expression vector, which can be used in plants, Rhizobium radiobacter (R. radiobacter) was transformed.
Arabidopsis thaliana (A. thaliana) is the most important model organism in plant science. It is easy to transform via R. radiobacter and has a short regeneration time. For the transformation of A. thaliana we chose the floral dip method. Therefore plants with inflorescence were required. Also a liquid culture of the T4MBP-carrying R. radiobacter was obligatory. This form of transformation is very simple, because we just had to dip the plants with the inflorescence into an infiltration medium, which contains R. radiobacter. After three weeks the plants were withered an we collected their seeds. To select the transgenic seeds all seeds were construed on selection medium.
In the future we want to raise the transgenic seeds and plant A. thaliana on heavy metal containing medium to proof the function of our T4MBP.
 |  |  |
Although Arabidopsis thaliana is, for a plant, easy and quick to regenerate, it still takes way more time (about 6 weeks) than the transformation of bacteria. So you will need a good timetable while working with plants!
