Template:Team:USyd-Australia/Calendar/Events List

From 2014.igem.org

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start: new Date(2014,7,28),
start: new Date(2014,7,28),
description: "Details: Ligation using T4 ligase and products. Incubated for 1 hr at room temperature and them left overnight in 4C. Enzymes heat-killed at 65C.<br><br>-  Tom"},
description: "Details: Ligation using T4 ligase and products. Incubated for 1 hr at room temperature and them left overnight in 4C. Enzymes heat-killed at 65C.<br><br>-  Tom"},
 +
{ title: "Construction of pUS204, Transformaiton of ligated products into Top10 cells",
 +
start: new Date(2014,8,01),
 +
description: "Details: Transformation and then incubated overnight at 37 C<br><br>-  Abi and Tom"},
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{ title: "Checking pUS201 construct, Re-checking the pUS201 by restriction digestion",
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start: new Date(2014,8,04),
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description: "Details: Restriction digestion (EcoR1, NdeI, NdeI/BamHI, NdeI/HindIII)<br><br>"},
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{ title: "construction of PUS204, Junction PCR to check whether pUS204 has been ligated successfully",
 +
start: new Date(2014,8,09),
 +
description: "Details: Junction PCR with iGEM1407/iGEM1408 primers and Taq<br><br>Result: Products plated for…why?<br><br>-  Tom"},
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{ title: "Construction of pUS203, Checking: heat-killing and running a gel of products",
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start: new Date(2014,8,10),
 +
description: "Details: Heat killed construct at 80 C for 20 min. Ran a gel electrophoresis of products wfor 100V for 1hr.<br><br>Result: Gel failed.<br><br>Conclusion: Gibson Assembly attempted anyway, gel fail was not indication of no construct<br><br>-  Tom"},
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{ title: "Construction of pUS201, Plating out Pus201 Gibson Assembly",
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start: new Date(2014,8,10),
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description: "Details: Plated out GA products onto Amp plates.<br><br>-  Tom"},
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{ title: "Construction of pUS204, Junction PCR gel",
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start: new Date(2014,8,10),
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description: "Details: Gel electrophoresis with PCR products (overnight pSB1C3/aeBlue cultures, 1hr growth cultures, no plasmid colony, no template control)<br><br>Result: ??<br><br>Conclusion: ??<br><br>"},
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{ title: "Checkin pUS203, Patch plate Int1I-2 and SamR construct",
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start: new Date(2014,8,11),
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description: "Details: GA product cultures transferred to patch plates using 25x \"small\" colonies and 25x\"large\" colonies.<br><br>Result: Colonies to be screened with junction PCR. Why patch plate?<br><br>Conclusion: ?<br><br>-  ?"},
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{ title: "Checking Pus203, Preperation of colony PCR of PUS203: of the \"small\" and \"large\" colonies on patch plate",
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start: new Date(2014,8,12),
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description: "Details: PCR with Hs463a and Hs464 primers and Taq.<br><br>"},
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{ title: "Checking Pus203, colony PCR of PUS203: of the \"small\" and \"large\" colonies on patch plate",
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start: new Date(2014,8,15),
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description: "Details: PCR with Hs463a and Hs464 primers and Taq.<br><br>Result: Expected fragment size was 473bp. No data received. ????<br><br>Conclusion: ?? Re-do?<br><br>-  rokiah"},
]       
]       
});
});

Latest revision as of 02:33, 15 October 2014

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