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Team:NCTU Formosa/Notebook/test5
From 2014.igem.org
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<div class="calendar"> | <div class="calendar"> | ||
<ul class="calendar-week"> | <ul class="calendar-week"> | ||
| - | <li> | + | <li>Sun.</li> |
| - | <li> | + | <li>Mon.</li> |
| - | <li> | + | <li>Tue.</li> |
| - | <li> | + | <li>Wed.</li> |
| - | <li> | + | <li>Thu.</li> |
| - | <li> | + | <li>Fri.</li> |
| - | <li> | + | <li>Sat.</li> |
</ul> | </ul> | ||
<ul class="calendar-day"> | <ul class="calendar-day"> | ||
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</ul> | </ul> | ||
</div> | </div> | ||
| - | <div class="calendar-note"> | + | <div class="calendar-note" style="height:251px;"> |
| - | <div class="single note"> | + | <div class="single note"> |
| + | <font color="#FF0000"><span style="font-size:1cm;">None</span></font><br> | ||
<br></div> | <br></div> | ||
<div class="single note"> | <div class="single note"> | ||
| - | + | <font color="#FF0000"><span style="font-size:1cm;">None</span></font><br> | |
<br></div> | <br></div> | ||
<div class="single note"> | <div class="single note"> | ||
| Line 76: | Line 77: | ||
<font color="#FF0000"><span style="font-size:1cm;">8/11</span></font><br></p> | <font color="#FF0000"><span style="font-size:1cm;">8/11</span></font><br></p> | ||
<p> | <p> | ||
| - | Transformation of NCTU1 (G1)</p> | + | <font color="yellow">Transformation of</font> NCTU1 (G1)</p> |
<p> | <p> | ||
| - | Transformation of PBAN (G2)</p> | + | <font color="yellow">Transformation of</font> PBAN (G2)</p> |
<p> | <p> | ||
<font color="#FF0000"><span style="font-size:1cm;">8/12</span></font><br></p> | <font color="#FF0000"><span style="font-size:1cm;">8/12</span></font><br></p> | ||
<p> | <p> | ||
| - | Cultivation of [PBAN-4]Kr,[PBAN-5]Krand [PBAN-6]Kr E.coli in LB broth (G1)</p> | + | <font color="yellow">Cultivation of</font> [PBAN-4]Kr,[PBAN-5]Krand [PBAN-6]Kr E.coli in LB broth (G1)</p> |
<p> | <p> | ||
| - | Cultivation of [RBS+BFP+J61048]Cr ,[T7+RBS+BFP+J61048]Ar Kr</p> | + | <font color="yellow">Cultivation of </font> [RBS+BFP+J61048]Cr ,[T7+RBS+BFP+J61048]Ar Kr</p> |
<p> | <p> | ||
| - | Mini-prep of [ | + | <font color="yellow">Mini-prep of</font> [P<sub>cons</sub>]Ar</p> |
<p> | <p> | ||
| - | Digestion of [ | + | <font color="yellow">Digestion of</font> [P<sub>cons</sub>]Ar (ES) (G2)</p> |
<p> | <p> | ||
<font color="#FF0000"><span style="font-size:1cm;">8/13</span></font><br></p> | <font color="#FF0000"><span style="font-size:1cm;">8/13</span></font><br></p> | ||
<p> | <p> | ||
| - | Mini-prep of [PBAN-4] Kr,[PBAN-5] Krand [PBAN-6] Kr (G1)</p> | + | <font color="yellow">Mini-prep of</font> [PBAN-4] Kr,[PBAN-5] Krand [PBAN-6] Kr (G1)</p> |
<p> | <p> | ||
| - | Digestion of [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1)</p> | + | <font color="yellow">Digestion of</font> [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1)</p> |
<p> | <p> | ||
| - | Electrophoresis of [ | + | <font color="yellow">Electrophoresis of</font> [P<sub>cons</sub>](mini)&(dig ES),digestion of [P<sub>cons</sub>](XP) |
| + | </p><p> | ||
| + | <font color="yellow">Ligation of</font> [P<sub>cons</sub>]+[RBS+BFP+J61048] (G2)</p> | ||
<p> | <p> | ||
<font color="#FF0000"><span style="font-size:1cm;">8/14</span></font><br></p> | <font color="#FF0000"><span style="font-size:1cm;">8/14</span></font><br></p> | ||
<p> | <p> | ||
| - | Electrophoresis of plasmid and digestion products of [PBAN-4] Kr(EP),[PBAN-5] Kr(EP)and [PBAN-6] Kr(EP) (G1)</p> | + | <font color="yellow">Electrophoresis of</font> plasmid and digestion products of [PBAN-4] Kr(EP),[PBAN-5] Kr(EP)and [PBAN-6] Kr(EP) (G1)</p> |
<p> | <p> | ||
| - | Purification from electrophoresis of[PBAN]No.4&5&6(G1)</p> | + | <font color="yellow">Purification from electrophoresis of</font> [PBAN]No.4&5&6(G1)</p> |
<p> | <p> | ||
| - | Transformation of the ligation product made yesterday</p> | + | <font color="yellow">Transformation of</font> the ligation product made yesterday</p> |
<p> | <p> | ||
| - | Electrophoresis of plasmid and digestion products of [PBAN]No.4&5&6,[ | + | <font color="yellow">Electrophoresis of</font> plasmid and digestion products of [PBAN]No.4&5&6,[P<sub>cons</sub>],[P<sub>cons</sub>+RBS+BFP+J61048] (G2)</p> |
<p> | <p> | ||
<font color="#FF0000"><span style="font-size:1cm;">8/15</span></font><br></p> | <font color="#FF0000"><span style="font-size:1cm;">8/15</span></font><br></p> | ||
<p> | <p> | ||
| - | Digestion of purification products yesterday:[PBAN]No.4&5&6 (EP) (G1)</p> | + | <font color="yellow">Digestion of</font> purification products yesterday:[PBAN]No.4&5&6 (EP) (G1)</p> |
<p> | <p> | ||
| - | Purification from electrophoresis of [PBAN]No.4&5&6</p> | + | <font color="yellow">Purification from electrophoresis of</font> [PBAN]No.4&5&6</p> |
<p> | <p> | ||
| - | Transformation of [B0034]Ar (G2)</p> | + | <font color="yellow">Transformation of</font> [B0034]Ar (G2)</p> |
<p> | <p> | ||
<font color="#FF0000"><span style="font-size:1cm;">8/16</span></font><br></p> | <font color="#FF0000"><span style="font-size:1cm;">8/16</span></font><br></p> | ||
<p> | <p> | ||
| - | Electrophoresis of the purification products from electrophoresis | + | <font color="yellow">Electrophoresis of</font> the purification products from electrophoresis,digestion products of[PBAN]No.4&5&6 (EP) (G1)</p> |
<p> | <p> | ||
| - | PCR of [B0034]</p> | + | <font color="yellow">PCR of</font> [B0034]</p> |
<p> | <p> | ||
| - | Electrophoresis of[PSB1K3](mini)&(dig E),[ | + | <font color="yellow">Electrophoresis of</font> [PSB1K3](mini)&(dig E),[P<sub>cons</sub>](dig SP),PCR products of B0034</p> |
<p> | <p> | ||
| - | Mini-prep of [B0034]Ar E.coli</p> | + | <font color="yellow">Mini-prep of</font> [B0034]Ar E.coli</p> |
<p> | <p> | ||
| - | Ligation of [ | + | <font color="yellow">Ligation of</font> [P<sub>cons</sub>]+[B0034] +PSB1K3</p> |
<p> | <p> | ||
| - | Digestion of [BFP](ES)</p> | + | <font color="yellow">Digestion of</font> [BFP](ES)</p> |
<p> | <p> | ||
| - | Electrophoresis of plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[ | + | <font color="yellow">Electrophoresis of </font> plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[P<sub>cons</sub>])</p> |
<br></div> | <br></div> | ||
<div class="single note"> | <div class="single note"> | ||
| Line 136: | Line 139: | ||
<font color="#FF0000"><span style="font-size:1cm;">8/17</span></font><br></p> | <font color="#FF0000"><span style="font-size:1cm;">8/17</span></font><br></p> | ||
<p> | <p> | ||
| - | Electrophoresis of the digestion products of[PBAN]No.4&5&6 (EP) (G1)</p> | + | <font color="yellow">Electrophoresis of</font> the digestion products of[PBAN]No.4&5&6 (EP) (G1)</p> |
<br></div> | <br></div> | ||
<div class="single note"> | <div class="single note"> | ||
Latest revision as of 14:24, 17 October 2014
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None
None
8/11
Transformation of NCTU1 (G1)
Transformation of PBAN (G2)
8/12
Cultivation of [PBAN-4]Kr,[PBAN-5]Krand [PBAN-6]Kr E.coli in LB broth (G1)
Cultivation of [RBS+BFP+J61048]Cr ,[T7+RBS+BFP+J61048]Ar Kr
Mini-prep of [Pcons]Ar
Digestion of [Pcons]Ar (ES) (G2)
8/13
Mini-prep of [PBAN-4] Kr,[PBAN-5] Krand [PBAN-6] Kr (G1)
Digestion of [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1)
Electrophoresis of [Pcons](mini)&(dig ES),digestion of [Pcons](XP)
Ligation of [Pcons]+[RBS+BFP+J61048] (G2)
8/14
Electrophoresis of plasmid and digestion products of [PBAN-4] Kr(EP),[PBAN-5] Kr(EP)and [PBAN-6] Kr(EP) (G1)
Purification from electrophoresis of [PBAN]No.4&5&6(G1)
Transformation of the ligation product made yesterday
Electrophoresis of plasmid and digestion products of [PBAN]No.4&5&6,[Pcons],[Pcons+RBS+BFP+J61048] (G2)
8/15
Digestion of purification products yesterday:[PBAN]No.4&5&6 (EP) (G1)
Purification from electrophoresis of [PBAN]No.4&5&6
Transformation of [B0034]Ar (G2)
8/16
Electrophoresis of the purification products from electrophoresis,digestion products of[PBAN]No.4&5&6 (EP) (G1)
PCR of [B0034]
Electrophoresis of [PSB1K3](mini)&(dig E),[Pcons](dig SP),PCR products of B0034
Mini-prep of [B0034]Ar E.coli
Ligation of [Pcons]+[B0034] +PSB1K3
Digestion of [BFP](ES)
Electrophoresis of plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[Pcons])
8/17
Electrophoresis of the digestion products of[PBAN]No.4&5&6 (EP) (G1)
None
None
