Team:Wageningen UR/notebook/protocols
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<h1>Protocols</h1> | <h1>Protocols</h1> | ||
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- | <p> | + | <p>All protocols used by the team are listed on this page and explained in PDF files. We have used standard iGEM protocols and protocols that we derived from literature or designed ourselves. </p> |
- | + | <br> | |
<section id="standard"> | <section id="standard"> | ||
<h2>Custom Protocols</h2> | <h2>Custom Protocols</h2> | ||
<br> | <br> | ||
- | <h3><a href="https://static.igem.org/mediawiki/2014/8/87/Wageningen_UR_protocols_ammoniumanalysis.pdf">Ammonium Analysis</a></h3> | + | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/8/87/Wageningen_UR_protocols_ammoniumanalysis.pdf" target="_blank">Ammonium Analysis</a></h3> |
- | <h3><a href="https://static.igem.org/mediawiki/2014/0/07/Wageningen_UR_protocols_DAPGprotocols.pdf">DAPG Protocols</a></h3> | + | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/0/07/Wageningen_UR_protocols_DAPGprotocols.pdf" target="_blank">DAPG Protocols</a></h3> |
- | <h3><a href="https://static.igem.org/mediawiki/2014/1/14/Wageningen_UR_protocols_FAgrowth.pdf">Fusarium Inhibition</a></h3> | + | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/1/14/Wageningen_UR_protocols_FAgrowth.pdf" target="_blank">Fusarium Inhibition</a></h3> |
- | <h3><a href="https://static.igem.org/mediawiki/2014/4/4b/Wageningen_UR_protocols_MgLgrowth.pdf">MgL Growth Protocol</a></h3> | + | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/4/4b/Wageningen_UR_protocols_MgLgrowth.pdf" target="_blank">MgL Growth Protocol</a></h3> |
- | <h3><a href="https://static.igem.org/mediawiki/2014/7/7a/Wageningen_UR_protocols_Pyoverdinegrowth.pdf">Pyoverdine | + | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/7/7a/Wageningen_UR_protocols_Pyoverdinegrowth.pdf" target="_blank">Pyoverdine Measurement Protocol</a></h3> |
- | <h3><a href="https://static.igem.org/mediawiki/2014/2/23/Wageningen_UR_protocols_Fusariuminhibition.pdf">Fusaric Acid inducible promoter growth experiment protocol</a></h3> | + | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/2/23/Wageningen_UR_protocols_Fusariuminhibition.pdf"target="_blank">Fusaric Acid inducible promoter growth experiment protocol</a></h3> |
- | <h3><a href="https://static.igem.org/mediawiki/2014/8/8e/Wageningen_UR_protocols_Promotercharacterisation.pdf">Promoter Characterisation Protocol</a></h3> | + | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/7/7d/Wageningen_UR_protocols_Metabolicmodelingpathways.pdf" target="_blank">Metabolic Modeling Pathways</a></h3> |
+ | |||
+ | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/8/8e/Wageningen_UR_protocols_Promotercharacterisation.pdf" target="_blank">Promoter Characterisation Protocol</a></h3> | ||
+ | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/3/32/Wageningen_UR_protocols_Promoterassembly.pdf" target="_blank">Promoter Assembly</a></h3> | ||
+ | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/1/14/Wageningen_UR_protocols_congoredassay.pdf" target="_blank">Congo Red Assay</a></h3><p style="padding-left:40px;">Collaboration protocol from iGEM Delft-Leiden 2014</p> | ||
</section> | </section> | ||
- | + | <br> | |
</section id="original"> | </section id="original"> | ||
<h2>Standard Protocols</h2> | <h2>Standard Protocols</h2> | ||
<br> | <br> | ||
- | <h3><a href="https://static.igem.org/mediawiki/2014/d/d0/Wageningen_UR_protocols_Mediabuffers.pdf">Media & Buffers</a></h3> | + | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/d/d0/Wageningen_UR_protocols_Mediabuffers.pdf" target="_blank">Media & Buffers</a></h3> |
- | <h3><a href="https://static.igem.org/mediawiki/2014/8/8f/Wageningen_UR_protocols_Transformations.pdf">Transformations</a></h3> | + | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/8/8f/Wageningen_UR_protocols_Transformations.pdf" target="_blank">Transformations</a></h3> |
- | <h3><a href="https://static.igem.org/mediawiki/2014/1/12/Wageningen_UR_protocols_Usingkits.pdf"> | + | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/1/12/Wageningen_UR_protocols_Usingkits.pdf" target="_blank">Used Kits</a></h3> |
- | <h3><a href="https://static.igem.org/mediawiki/2014/e/eb/Wageningen_UR_protocols_Pcr.pdf">PCR</a></h3> | + | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/e/eb/Wageningen_UR_protocols_Pcr.pdf" target="_blank">PCR</a></h3> |
- | <h3><a href="https://static.igem.org/mediawiki/2014/1/15/Wageningen_UR_protocols_Restrictionligation.pdf">Restrictions & Ligations</a></h3> | + | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/1/15/Wageningen_UR_protocols_Restrictionligation.pdf" target="_blank">Restrictions & Ligations</a></h3> |
- | <h3><a href="https://static.igem.org/mediawiki/2014/b/b5/Wageningen_UR_protocols_Site_Directed_Mutagenesis.pdf">Mutagenesis</a></h3> | + | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/b/b5/Wageningen_UR_protocols_Site_Directed_Mutagenesis.pdf" target="_blank">Mutagenesis</a></h3> |
</section> | </section> | ||
- | + | <br> | |
- | + | <p style="float:right"><b>Continue to <a href="https://2014.igem.org/Team:Wageningen_UR/notebook/links" class="soft_link">Interesting links >></a> </b> | |
</html> | </html> | ||
{{:Team:Wageningen_UR/templates/footer}} | {{:Team:Wageningen_UR/templates/footer}} |
Latest revision as of 03:51, 18 October 2014
Protocols
All protocols used by the team are listed on this page and explained in PDF files. We have used standard iGEM protocols and protocols that we derived from literature or designed ourselves.
Custom Protocols
Ammonium Analysis
DAPG Protocols
Fusarium Inhibition
MgL Growth Protocol
Pyoverdine Measurement Protocol
Fusaric Acid inducible promoter growth experiment protocol
Metabolic Modeling Pathways
Promoter Characterisation Protocol
Promoter Assembly
Congo Red Assay
Collaboration protocol from iGEM Delft-Leiden 2014
Standard Protocols
Media & Buffers
Transformations
Used Kits
PCR
Restrictions & Ligations
Mutagenesis
Continue to Interesting links >>