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Team:Paris Saclay/Notebook/September/16
From 2014.igem.org
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{{Team:Paris_Saclay/notebook_header}} | {{Team:Paris_Saclay/notebook_header}} | ||
| + | =Tuesday 16th September= | ||
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==Lab Work== | ==Lab Work== | ||
''By Mélanie'' | ''By Mélanie'' | ||
| - | === | + | ===Lemon scent=== |
====Plasmids extraction==== | ====Plasmids extraction==== | ||
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by PacI and PSTI | by PacI and PSTI | ||
| - | [[File:1609 digestion ps et gs par pacI.jpg|400px]] | + | [[File:1609 digestion ps et gs par pacI.jpg|400px|center]] |
(well 2 and 3 : PacI digestion) | (well 2 and 3 : PacI digestion) | ||
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Test of the transformation made [https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook/September/15#Transformation_with_the_ligation_.28futur_pPS2.2F4.29 yesterday] to know if we had obtained a plasmid] | Test of the transformation made [https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook/September/15#Transformation_with_the_ligation_.28futur_pPS2.2F4.29 yesterday] to know if we had obtained a plasmid] | ||
| - | [[File:1609 PCR sur clones.jpg|400px]] | + | [[File:1609 PCR sur clones.jpg|400px|center]] |
primer to ckeck the direction of the insert | primer to ckeck the direction of the insert | ||
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| - | === | + | ===Construction of the fusion protein (color)=== |
====PCR==== | ====PCR==== | ||
PCR to check if we have the insert in our topo plasmid | PCR to check if we have the insert in our topo plasmid | ||
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[https://2014.igem.org/Team:Paris_Saclay/Notebook/September/15#PCR see here] | [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/15#PCR see here] | ||
| - | [[File:1609 chromo topo 2-9 jaune 10-17 bleu.jpg|400px]] | + | [[File:1609 chromo topo 2-9 jaune 10-17 bleu.jpg|400px|center]] |
well 2-9 = chromo yellow in topo plasmid | well 2-9 = chromo yellow in topo plasmid | ||
Latest revision as of 12:35, 14 October 2014
Contents |
Tuesday 16th September
Lab Work
By Mélanie
Lemon scent
Plasmids extraction
Extraction of the plasmid from the culture made yesterday(PS)
Digestion of PS topo plasmids
by PacI and PSTI
(well 2 and 3 : PacI digestion)
Ligation
After digestion, ligation overnight in PPS1
PCR Colony
Test of the transformation made yesterday to know if we had obtained a plasmid]
primer to ckeck the direction of the insert PPS2 : iPS72-96
PPS4 : ips72-97
= no insert (just well 1-9)
liquide culture
liquide culture from yesterday's colony to make stock tomorrow
PCR cad to cloning in pSBIC3
PCR and digestion PSTI/ecoRI and ligation
PCR GS
PCR to check if we have the insert in our topo plasmid
Construction of the fusion protein (color)
PCR
PCR to check if we have the insert in our topo plasmid
well 2-9 = chromo yellow in topo plasmid well 10-17 = chromo blue in topo plasmid
Photo of the Day
Work of [http://ateliers-artistes-belleville.fr/artiste/therese-bichon/ Thérèse Bichon] , one of those few we have interviewed during this month of september.
To learn more about the ethic aspect of our project, just click here
