Template:Team:USyd-Australia/Calendar/Events List

{ title: 'Plasmid Prep psB6A1, Finish',

start: new Date(2014,8,7),

description: 'Result: Plasmid prep yielded 4x100uL tubes<br><br>-  Rokiah'},

{ title: 'Plasmid prep of pUS44, Checking: Setup restriction digest and incubate',

start: new Date(2014,8,7),

description: 'Details: restriction digest to check plasmids (EcoR1, Ssp1, null). Incubated at 37C overnight.<br><br>-  Abi'},

{ title: 'Plasmid prep of pUS44, Checking: finish digests, gel electrophoresis of digests',

start: new Date(2014,9,7),

description: 'Details: Gel electrophoresis (100V, 400mA, 30min)<br><br>Result: Gel broke. <br><br>-  Abi'},

{ title: 'Plasmid prep of psB6A1, Checking: finish digests, gel electrophoresis of digests',

start: new Date(2014,9,7),

description: 'Details: Gel electrophoresis (100V, 400mA, 30min)<br><br>Result: Gel broke. pSB6A1 yielded 5104.1ng/uL DNA (A260/280) 1.62.<br><br>Conclusion: Possible protein contamination to samples<br><br>-  Abi'},

{ title: 'Plasmid prep of pSB6A1, Re-doing digest',

start: new Date(2014,10,7),

description: 'Details: Restriction digest to check plasmids (EcoR1, EcoR1+Pst1, EcoR1+Nde1). <br><br>-  Jeanne'},

{ title: 'Plasmid prep of pUS44, Re-doing digest',

start: new Date(2014,14,7),

description: 'Details: Restriction digest to check plasmids (EcoR1, EcoR1+Pst1, EcoR1+Nde1). <br><br>-  Abi'},

{ title: 'Plasmid prep of pUS44, Re-doing digest',

start: new Date(2014,16,7),

description: 'Details: Restriction digest. Did the protocol change?<br><br>Conclusion: Nanospec: 395.5ng/mL DNA yield<br><br>-  Abi Tom'},

{ title: 'Construction of pUS201, PCR of pSB6A1 using iGEM401/402 primers ',

start: new Date(2014,24,7),

description: 'Details: PCR Master mix with 1401/1402 primers. Used iGEM pSB6A1. Ran on regular program PCR ~2HRS<br><br>Result: Yield 1018.6ng/uL and low 260/280<br><br>Conclusion: ?<br><br>-  Callum'},

{ title: 'Construction of pUS201, Checking: finish pcr, gel electrophoresis of digests',

start: new Date(2014,24,7),

description: 'Details: Gel electrophoresis (100V, 400mA, 1hr). Stained in gel red for 30min<br><br>Result: Expected fragment size of 2.6kB from pSB6A1<br><br>Conclusion: Primers amplified expected product from pSB6A1<br><br>-  Callum'},

{ title: 'Construction of pUS204, Restriction digest of pSB1C3',

start: new Date(2014,29,7),

description: 'Details: Restriction digest (EcoR1+Pst1)<br><br>Result: Yield 25ng/mL<br><br>Conclusion: Enough to use for gibson assembly<br><br>-  Callum'},

{ title: 'Construction of pUS201, PCR of pSB1C3 using iGEM403/1404 primers ',

start: new Date(2014,30,7),

description: 'Details: PCR Master mix with 1403/1404 primers. Used iGEM psB1C3. Ran on regular program PCR ~2HRS<br><br>Result: Yield 329.4ng/uL, low 260/280<br><br>-  Callum'},

{ title: 'Construction of pUS204, Transformation: pUS204 into top10 cells',

start: new Date(2014,30,7),

description: 'Details: Transformation (competent top10, pUS204). Incubated on AmpR plates for 2hrs<br><br>Result: Some AmpR colonies present, no blue colonies detected<br><br>Conclusion: pUS024 did not successfully transform, "far unlikely positive control worked" <br><br>-  ?'},

{ title: 'construction of pUS201, see notebook: second GA and transformation successful???',

start: new Date(2014,0,8),

description: ''},

{ title: 'construction of pUS201, re-doing pSB1C3 PCR with iGEM1403/1404',

start: new Date(2014,3,8),

description: 'Details: PCR Master mix with 1403/1404 primers. Used iGEM psB1C3. Ran on regular program PCR ~2HRS<br><br>Result: Products stored in the fridge<br><br>-  Jeanne Callum'},

{ title: 'construction of pus201, Gel purification: gel electrophoresis of pSB1C3 PCR ',

start: new Date(2014,4,8),

description: 'Details: Gel purification electrophoresis (60V, 400mA, 1hr). Stained in gel red??? for 30min<br><br>Result: missing gel??<br><br>-  Jeanne Callum'},

{ title: 'Checking pUS201 construct, Plasmid mini-Prep: to confirm pUS201 in top10 colonies',

start: new Date(2014,4,8),

description: 'Details: Incubated overnight to finish later<br><br>Result: Yield 5.7ng/ul, 1.61 260/280<br><br>Conclusion: Yield too low to use<br><br>-  Jeanne Callum'},

{ title: 'Checking IntI1 from SamR's construct, Restriction digest: SamR's pSB1C3+ARApBAD',

start: new Date(2014,7,8),

description: 'Details: Restriction digest (EcoR1+Pst1), incubated at 37 C for 1hr<br><br>Result: Yield: 4.46 ug/uL. Stored in -20 C fridge<br><br>Conclusion: Yield much higher than estimate provided by SamR (50ng/uL). Useable<br><br>-  Rokiah'},

{ title: 'Checking pUS201 construct, Checking pUS201 with digests',

start: new Date(2014,11,8),

description: 'Details: Restriction Digest (uncut, EcoR1, HindIII, HindIII+BamHI)<br><br>Result: gel????? Results???<br><br>-  ?'},

{ title: 'Checking IntI1 from SamR's construct, Checking: SamR's digests on gel electrohoresis',

start: new Date(2014,11,8),

description: 'Details: Gel electrophoresis (Spe1, uncut) at 120V for 1hr. Stained in gel red for ~30min.<br><br>Result: [12.08 SamR gel]. Cut lanes travelled further than uncut, as expected.<br><br>Conclusion: NOTE:: notes at the end cannot be read<br><br>-  Rokiah'},

{ title: 'Making IntI1 for pUS203, Restriction Digest: extracting SamR's AraC-pBAD from pSB1C3',

start: new Date(2014,12,8),

description: 'Details: pSB1C3 with AraC-pBAD was incubated with *specific enzyme* at 80 C for 20 min. The enzyme was heat-killed<br><br>-  andy'},

{ title: 'Construction of pUS204, Restrction digestion of aeBlue gBlock',

start: new Date(2014,13,8),

description: 'Details: Restriction Digestion (EcoR1+Pst1)<br><br>Result: Yield: 1.6ng/uL<br><br>Conclusion: Enough yield to continue with ligation<br><br>-  abi and tom'},

{ title: 'Checking pUS201 construct, Plasmid mini-Prep: to confirm pUS201 in top10 colonies',

start: new Date(2014,20,8),

description: 'Details: Plasmid prep<br><br>Result: Yield: 3680.1ng/uL. 2.03 A260/280<br><br>Conclusion: A260/280 high ratio, may have contamination<br><br>-  Callum'},

{ title: 'Construction of pUS204, Ligation of pSB1C3 and aeBlue gBlock digestion products',

start: new Date(2014,27,8),

description: 'Details: Ligation using T4 ligase and products. Incubated for 1 hr at room temperature and them left overnight in 4C. Enzymes heat-killed at 65C.<br><br>-  Tom'},

{ title: 'Construction of pUS204, Transformaiton of ligated products into Top10 cells',

start: new Date(2014,0,9),

description: 'Details: Transformation and then incubated overnight at 37 C<br><br>-  Abi and Tom'},

{ title: 'Checking pUS201 construct, Re-checking the pUS201 by restriction digestion',

start: new Date(2014,3,9),

description: 'Details: Restriction digestion (EcoR1, NdeI, NdeI/BamHI, NdeI/HindIII)<br><br>'},

{ title: 'construction of PUS204, Junction PCR to check whether pUS204 has been ligated successfully',

start: new Date(2014,8,9),

description: 'Details: Junction PCR with iGEM1407/iGEM1408 primers and Taq<br><br>Result: Products plated for... why?<br><br>-  Tom'},

{ title: 'Construction of pUS203, Checking: heat-killing and running a gel of products',

start: new Date(2014,9,9),

description: 'Details: Heat killed construct at 80 C for 20 min. Ran a gel electrophoresis of products wfor 100V for 1hr.<br><br>Result: Gel failed.<br><br>Conclusion: Gibson Assembly attempted anyway, gel fail was not indication of no construct<br><br>-  Tom'},

{ title: 'Construction of pUS201, Plating out Pus201 Gibson Assembly',

start: new Date(2014,9,9),

description: 'Details: Plated out GA products onto Amp plates.<br><br>-  Tom'},

{ title: 'Construction of pUS204, Junction PCR gel',

start: new Date(2014,9,9),

description: 'Details: Gel electrophoresis with PCR products (overnight pSB1C3/aeBlue cultures, 1hr growth cultures, no plasmid colony, no template control)<br><br>Result: ??<br><br>Conclusion: ??<br><br>'},

{ title: 'Checkin pUS203, Patch plate Int1I-2 and SamR construct',

start: new Date(2014,10,9),

description: 'Details: GA product cultures transferred to patch plates using 25x "small" colonies and 25x"large" colonies.<br><br>Result: Colonies to be screened with junction PCR. Why patch plate?<br><br>Conclusion: ?<br><br>-  ?'},

{ title: 'Checking Pus203, Preperation of colony PCR of PUS203: of the "small" and "large" colonies on patch plate',

start: new Date(2014,11,9),

description: 'Details: PCR with Hs463a and Hs464 primers and Taq.<br><br>'},

{ title: 'Checking Pus203, colony PCR of PUS203: of the "small" and "large" colonies on patch plate',

start: new Date(2014,14,9),

description: 'Details: PCR with Hs463a and Hs464 primers and Taq.<br><br>Result: Expected fragment size was 473bp. No data received. ????<br><br>Conclusion: ?? Re-do?<br><br>-  rokiah'},